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The map label for this gene is alaS

Identifier: 82751218

GI number: 82751218

Start: 1601320

End: 1603950

Strand: Reverse

Name: alaS

Synonym: SAB1489c

Alternate gene names: 82751218

Gene position: 1603950-1601320 (Counterclockwise)

Preceding gene: 82751219

Following gene: 82751217

Centisome position: 58.48

GC content: 35.23

Gene sequence:

>2631_bases
ATGAAAAAGTTAAAAGCGAGTGAAATTAGACAAAAATATCTAGATTTCTTTGTTGAAAAAGGACATATGGTTGAACCTTC
TGCACCATTAGTGCCAATTGATGATGATACATTATTATGGATTAATTCAGGTGTAGCAACATTAAAGAAATATTTTGATG
GACGTGAAACACCTAAAAAGCCAAGAATTGTAAACTCTCAAAAAGCTATTCGTACAAATGATATTGAAAATGTTGGTTTC
ACAGCGCGTCACCATACATTCTTTGAAATGTTAGGTAACTTCTCAATTGGTGATTACTTTAAACAAGAAGCGATTGAATT
TGCTTGGGAATTTTTAACGAGTGATAAATGGATGGGTATGGAGCCAGATAAATTGTACGTTACGATTCATCCGGAAGATA
TGGAAGCATACAACATTTGGCATAAAGATATTGGGCTTGAAGAAAGTCGCATTATTCGCATTGAAGGTAACTTCTGGGAT
ATTGGTGAAGGGCCTTCAGGACCGAACACTGAGATTTTCTATGATCGCGGAGAAGCATATGGACAAGACGATCCGGCAGA
AGAAATGTATCCAGGTGGAGAAAATGAACGCTATCTTGAAGTATGGAACTTAGTATTTAGTGAATTCAATCATAATAAAG
ATCATAGTTACACACCATTACCTAATAAAAACATTGATACCGGCATGGGGCTTGAGCGTATGGCCTCAGTTTCTCAAAAT
GTACGTACTAACTATGAAACAGATTTATTTATGCCTATAATGAATGAAATCGAAAAAGTATCAGGTAAACAATATTTAGT
AAACAACGAACAAGATGTGGCATTTAAAGTAATTGCTGACCACATTCGTACGATTGCATTTGCAATTTCTGATGGTGCAT
TACCTGCCAATGAAGGTAGAGGATATGTATTACGTCGATTGTTACGTCGTGCCGTTCGTTTTAGTCAAACGTTAGGAATC
AATGAGCCATTTATGTACAAACTCGTTGATATTGTTGCAGACATTATGGAACCATATTATCCAAATGTTAAGGAAAAAGC
AGATTTCATTAAGCGTGTTATAAAGTCTGAAGAAGAACGATTCCATGAAACATTAGAAGATGGTTTAGCGATTTTAAATG
AATTAATTAAAAAAGCTAAAGCAACAACAAATGAAATTAATGGGAAAGATGCATTTAAATTGTATGATACGTACGGGTTC
CCAATTGAATTAACTGAAGAAATAGCAGTGCAAGCAGGATTGAAAGTTGATATGACAACATTCGAGTCAGAAATGCAACA
ACAACGTGATCGTGCACGTCAAGCACGTCAAAATTCTCAATCAATGCAAGTTCAAAGTGAAGTATTGAAAAATATTACAT
CTGCAAGTACTTTTGTTGGTTATGATACTGCGACAGCTCAAACAACACTAACACACTTGATATATAATGGTGAAGAAGTT
TCACAAGTTGAAGCGGGTGAAACAGTATACTTCATGTTAACGGAAACACCATTTTATGCAGTCAGTGGTGGACAAGTTGC
GGATACAGGTATTGTTTATAATGACAATTTTGAAATTGCTGTTAGTGAAGTAACCAAAGCACCAAATGGTCAAAACTTGC
ATAAAGGAGCAGTACAATTTGGCCAAGTAAATGTTGGCGCTACAGTGTCTGCTGAAGTGAACCAAAATGATCGACGTGAC
ATTCAAAAGAACCATAGTGCAACACATTTATTACATGCAGCGTTGAAATCAGTACTGGGTGATCATGTTAACCAAGCTGG
TTCACTAGTAGAAGCAGATCGTTTACGTTTTGATTTCTCTCATTTTGGTCCAATGACTAATGATGAAATTGATCAAGTTG
AACGCTTAGTAAATGAAGAAATTTGGAAAGGTATTGACGTTAACATTCAAGAAATGGATATTGCTTCAGCTAAAGAAATG
GGCGCAATGGCATTATTCGGTGAAAAATATGGTGATGTTGTGCGTGTAGTAAATATGGCACCATTTTCAATTGAATTATG
TGGTGGTATTCATGTCCGCAATACTTCTGAAATTGGCTTATTCAAAATAGTAAGTGAGTCAGGTACAGGAGCTGGTGTGC
GTCGTATTGAAGCATTAACAGGTAAAGCAGCTTTCTTATATTTAGAAGATATTCAAGAGAAATTTAATACGATGAAATCA
CAGATGAAAGTGAAATCTGATGATCAAGTAATCGATAAGTTAACACAATTGCAAGATGAAGAAAAAGCATTATTAAAACA
ATTAGAGCAACGTGACAAAGAAATCACATCACTTAAAATGGGTAATATTGAAGATCAAGTTGAAGAAATCAATGGCTATA
AAGTATTGGTTACTGAAGTGGATGTACCAAATGCGAAAGCAATTCGCTCGACAATGGACGATTTTAAATCTAAACTACAA
GATACAATTATCATTCTTGCAAGTAATGTTGATGATAAAGTATCGATGGTTGCAACTGTCCCTAAATCTTTAACAAATAA
TGTTAAAGCCGGTGATCTTATCAAACAAATGGCACCAATCGTTAGTGGTAAAGGTGGCGGTCGTCCAGATATGGCTCAAG
GTGGCGGTACACAACCTGAAAATATCTCAAAATCATTAAGCTTTATTAAAGATTACATTAAAAATCTATAA

Upstream 100 bases:

>100_bases
AAAAGTGATATTGATTTATACTAGTTTTTCAAATCGACTATCTGTGTCATCAATTTTTATGGAATGGTAATTTAAAAACA
GTAAATAGGATGTGTAAATG

Downstream 100 bases:

>100_bases
TTTAAAGCCATAATATTGTATGATGATAGTGAGTTATATTTGTAATTAAAGGAGTGTCGCAAATGGAAAACTTTGATAAA
ACAATGAAATTCGACTATGA

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 876; Mature: 876

Protein sequence:

>876_residues
MKKLKASEIRQKYLDFFVEKGHMVEPSAPLVPIDDDTLLWINSGVATLKKYFDGRETPKKPRIVNSQKAIRTNDIENVGF
TARHHTFFEMLGNFSIGDYFKQEAIEFAWEFLTSDKWMGMEPDKLYVTIHPEDMEAYNIWHKDIGLEESRIIRIEGNFWD
IGEGPSGPNTEIFYDRGEAYGQDDPAEEMYPGGENERYLEVWNLVFSEFNHNKDHSYTPLPNKNIDTGMGLERMASVSQN
VRTNYETDLFMPIMNEIEKVSGKQYLVNNEQDVAFKVIADHIRTIAFAISDGALPANEGRGYVLRRLLRRAVRFSQTLGI
NEPFMYKLVDIVADIMEPYYPNVKEKADFIKRVIKSEEERFHETLEDGLAILNELIKKAKATTNEINGKDAFKLYDTYGF
PIELTEEIAVQAGLKVDMTTFESEMQQQRDRARQARQNSQSMQVQSEVLKNITSASTFVGYDTATAQTTLTHLIYNGEEV
SQVEAGETVYFMLTETPFYAVSGGQVADTGIVYNDNFEIAVSEVTKAPNGQNLHKGAVQFGQVNVGATVSAEVNQNDRRD
IQKNHSATHLLHAALKSVLGDHVNQAGSLVEADRLRFDFSHFGPMTNDEIDQVERLVNEEIWKGIDVNIQEMDIASAKEM
GAMALFGEKYGDVVRVVNMAPFSIELCGGIHVRNTSEIGLFKIVSESGTGAGVRRIEALTGKAAFLYLEDIQEKFNTMKS
QMKVKSDDQVIDKLTQLQDEEKALLKQLEQRDKEITSLKMGNIEDQVEEINGYKVLVTEVDVPNAKAIRSTMDDFKSKLQ
DTIIILASNVDDKVSMVATVPKSLTNNVKAGDLIKQMAPIVSGKGGGRPDMAQGGGTQPENISKSLSFIKDYIKNL

Sequences:

>Translated_876_residues
MKKLKASEIRQKYLDFFVEKGHMVEPSAPLVPIDDDTLLWINSGVATLKKYFDGRETPKKPRIVNSQKAIRTNDIENVGF
TARHHTFFEMLGNFSIGDYFKQEAIEFAWEFLTSDKWMGMEPDKLYVTIHPEDMEAYNIWHKDIGLEESRIIRIEGNFWD
IGEGPSGPNTEIFYDRGEAYGQDDPAEEMYPGGENERYLEVWNLVFSEFNHNKDHSYTPLPNKNIDTGMGLERMASVSQN
VRTNYETDLFMPIMNEIEKVSGKQYLVNNEQDVAFKVIADHIRTIAFAISDGALPANEGRGYVLRRLLRRAVRFSQTLGI
NEPFMYKLVDIVADIMEPYYPNVKEKADFIKRVIKSEEERFHETLEDGLAILNELIKKAKATTNEINGKDAFKLYDTYGF
PIELTEEIAVQAGLKVDMTTFESEMQQQRDRARQARQNSQSMQVQSEVLKNITSASTFVGYDTATAQTTLTHLIYNGEEV
SQVEAGETVYFMLTETPFYAVSGGQVADTGIVYNDNFEIAVSEVTKAPNGQNLHKGAVQFGQVNVGATVSAEVNQNDRRD
IQKNHSATHLLHAALKSVLGDHVNQAGSLVEADRLRFDFSHFGPMTNDEIDQVERLVNEEIWKGIDVNIQEMDIASAKEM
GAMALFGEKYGDVVRVVNMAPFSIELCGGIHVRNTSEIGLFKIVSESGTGAGVRRIEALTGKAAFLYLEDIQEKFNTMKS
QMKVKSDDQVIDKLTQLQDEEKALLKQLEQRDKEITSLKMGNIEDQVEEINGYKVLVTEVDVPNAKAIRSTMDDFKSKLQ
DTIIILASNVDDKVSMVATVPKSLTNNVKAGDLIKQMAPIVSGKGGGRPDMAQGGGTQPENISKSLSFIKDYIKNL
>Mature_876_residues
MKKLKASEIRQKYLDFFVEKGHMVEPSAPLVPIDDDTLLWINSGVATLKKYFDGRETPKKPRIVNSQKAIRTNDIENVGF
TARHHTFFEMLGNFSIGDYFKQEAIEFAWEFLTSDKWMGMEPDKLYVTIHPEDMEAYNIWHKDIGLEESRIIRIEGNFWD
IGEGPSGPNTEIFYDRGEAYGQDDPAEEMYPGGENERYLEVWNLVFSEFNHNKDHSYTPLPNKNIDTGMGLERMASVSQN
VRTNYETDLFMPIMNEIEKVSGKQYLVNNEQDVAFKVIADHIRTIAFAISDGALPANEGRGYVLRRLLRRAVRFSQTLGI
NEPFMYKLVDIVADIMEPYYPNVKEKADFIKRVIKSEEERFHETLEDGLAILNELIKKAKATTNEINGKDAFKLYDTYGF
PIELTEEIAVQAGLKVDMTTFESEMQQQRDRARQARQNSQSMQVQSEVLKNITSASTFVGYDTATAQTTLTHLIYNGEEV
SQVEAGETVYFMLTETPFYAVSGGQVADTGIVYNDNFEIAVSEVTKAPNGQNLHKGAVQFGQVNVGATVSAEVNQNDRRD
IQKNHSATHLLHAALKSVLGDHVNQAGSLVEADRLRFDFSHFGPMTNDEIDQVERLVNEEIWKGIDVNIQEMDIASAKEM
GAMALFGEKYGDVVRVVNMAPFSIELCGGIHVRNTSEIGLFKIVSESGTGAGVRRIEALTGKAAFLYLEDIQEKFNTMKS
QMKVKSDDQVIDKLTQLQDEEKALLKQLEQRDKEITSLKMGNIEDQVEEINGYKVLVTEVDVPNAKAIRSTMDDFKSKLQ
DTIIILASNVDDKVSMVATVPKSLTNNVKAGDLIKQMAPIVSGKGGGRPDMAQGGGTQPENISKSLSFIKDYIKNL

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=975, Percent_Identity=34.0512820512821, Blast_Score=484, Evalue=1e-136,
Organism=Homo sapiens, GI38569417, Length=959, Percent_Identity=28.4671532846715, Blast_Score=348, Evalue=2e-95,
Organism=Escherichia coli, GI1789048, Length=882, Percent_Identity=45.2380952380952, Blast_Score=762, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17506981, Length=969, Percent_Identity=33.6429308565531, Blast_Score=448, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI17536681, Length=791, Percent_Identity=32.2376738305942, Blast_Score=351, Evalue=9e-97,
Organism=Saccharomyces cerevisiae, GI6324911, Length=964, Percent_Identity=35.1659751037344, Blast_Score=492, Evalue=1e-140,
Organism=Drosophila melanogaster, GI24582809, Length=967, Percent_Identity=34.8500517063082, Blast_Score=485, Evalue=1e-137,
Organism=Drosophila melanogaster, GI45552267, Length=967, Percent_Identity=34.8500517063082, Blast_Score=485, Evalue=1e-137,
Organism=Drosophila melanogaster, GI24658214, Length=836, Percent_Identity=27.9904306220096, Blast_Score=306, Evalue=4e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 98542; Mature: 98542

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLKASEIRQKYLDFFVEKGHMVEPSAPLVPIDDDTLLWINSGVATLKKYFDGRETPKK
CCCCCHHHHHHHHHHHHHHCCCEECCCCCEEECCCCEEEEECCCHHHHHHHHCCCCCCCC
PRIVNSQKAIRTNDIENVGFTARHHTFFEMLGNFSIGDYFKQEAIEFAWEFLTSDKWMGM
CCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
EPDKLYVTIHPEDMEAYNIWHKDIGLEESRIIRIEGNFWDIGEGPSGPNTEIFYDRGEAY
CCCEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCEEECCCCCCCCCCCEEEECCCCC
GQDDPAEEMYPGGENERYLEVWNLVFSEFNHNKDHSYTPLPNKNIDTGMGLERMASVSQN
CCCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
VRTNYETDLFMPIMNEIEKVSGKQYLVNNEQDVAFKVIADHIRTIAFAISDGALPANEGR
HCCCCCCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCC
GYVLRRLLRRAVRFSQTLGINEPFMYKLVDIVADIMEPYYPNVKEKADFIKRVIKSEEER
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
FHETLEDGLAILNELIKKAKATTNEINGKDAFKLYDTYGFPIELTEEIAVQAGLKVDMTT
HHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEEECCCCCEEEHHHHHHHCCCEEEHHH
FESEMQQQRDRARQARQNSQSMQVQSEVLKNITSASTFVGYDTATAQTTLTHLIYNGEEV
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHCCCCHH
SQVEAGETVYFMLTETPFYAVSGGQVADTGIVYNDNFEIAVSEVTKAPNGQNLHKGAVQF
HHCCCCCEEEEEEECCCEEEECCCCEECCCEEECCCCEEEEHHHHCCCCCCCHHHHHHEE
GQVNVGATVSAEVNQNDRRDIQKNHSATHLLHAALKSVLGDHVNQAGSLVEADRLRFDFS
CEEECCEEEEECCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHEECHH
HFGPMTNDEIDQVERLVNEEIWKGIDVNIQEMDIASAKEMGAMALFGEKYGDVVRVVNMA
HCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHCHHHHHHHCHHHHHHHHHHHHHHHHCCC
PFSIELCGGIHVRNTSEIGLFKIVSESGTGAGVRRIEALTGKAAFLYLEDIQEKFNTMKS
CCEEEEECCEEECCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHH
QMKVKSDDQVIDKLTQLQDEEKALLKQLEQRDKEITSLKMGNIEDQVEEINGYKVLVTEV
HHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEE
DVPNAKAIRSTMDDFKSKLQDTIIILASNVDDKVSMVATVPKSLTNNVKAGDLIKQMAPI
CCCCHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
VSGKGGGRPDMAQGGGTQPENISKSLSFIKDYIKNL
HCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKLKASEIRQKYLDFFVEKGHMVEPSAPLVPIDDDTLLWINSGVATLKKYFDGRETPKK
CCCCCHHHHHHHHHHHHHHCCCEECCCCCEEECCCCEEEEECCCHHHHHHHHCCCCCCCC
PRIVNSQKAIRTNDIENVGFTARHHTFFEMLGNFSIGDYFKQEAIEFAWEFLTSDKWMGM
CCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
EPDKLYVTIHPEDMEAYNIWHKDIGLEESRIIRIEGNFWDIGEGPSGPNTEIFYDRGEAY
CCCEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCEEECCCCCCCCCCCEEEECCCCC
GQDDPAEEMYPGGENERYLEVWNLVFSEFNHNKDHSYTPLPNKNIDTGMGLERMASVSQN
CCCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
VRTNYETDLFMPIMNEIEKVSGKQYLVNNEQDVAFKVIADHIRTIAFAISDGALPANEGR
HCCCCCCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCC
GYVLRRLLRRAVRFSQTLGINEPFMYKLVDIVADIMEPYYPNVKEKADFIKRVIKSEEER
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
FHETLEDGLAILNELIKKAKATTNEINGKDAFKLYDTYGFPIELTEEIAVQAGLKVDMTT
HHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEEECCCCCEEEHHHHHHHCCCEEEHHH
FESEMQQQRDRARQARQNSQSMQVQSEVLKNITSASTFVGYDTATAQTTLTHLIYNGEEV
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHCCCCHH
SQVEAGETVYFMLTETPFYAVSGGQVADTGIVYNDNFEIAVSEVTKAPNGQNLHKGAVQF
HHCCCCCEEEEEEECCCEEEECCCCEECCCEEECCCCEEEEHHHHCCCCCCCHHHHHHEE
GQVNVGATVSAEVNQNDRRDIQKNHSATHLLHAALKSVLGDHVNQAGSLVEADRLRFDFS
CEEECCEEEEECCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHEECHH
HFGPMTNDEIDQVERLVNEEIWKGIDVNIQEMDIASAKEMGAMALFGEKYGDVVRVVNMA
HCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHCHHHHHHHCHHHHHHHHHHHHHHHHCCC
PFSIELCGGIHVRNTSEIGLFKIVSESGTGAGVRRIEALTGKAAFLYLEDIQEKFNTMKS
CCEEEEECCEEECCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHH
QMKVKSDDQVIDKLTQLQDEEKALLKQLEQRDKEITSLKMGNIEDQVEEINGYKVLVTEV
HHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEE
DVPNAKAIRSTMDDFKSKLQDTIIILASNVDDKVSMVATVPKSLTNNVKAGDLIKQMAPI
CCCCHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
VSGKGGGRPDMAQGGGTQPENISKSLSFIKDYIKNL
HCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA