Click here to switch to the map view.

The map label for this gene is ansA [H]

Identifier: 82751075

GI number: 82751075

Start: 1469107

End: 1470075

Strand: Direct

Name: ansA [H]

Synonym: SAB1341

Alternate gene names: 82751075

Gene position: 1469107-1470075 (Clockwise)

Preceding gene: 82751049

Following gene: 82751080

Centisome position: 53.57

GC content: 34.67

Gene sequence:

>969_bases
ATGAAACATCTACTTGTTATTCATACTGGTGGCACCATTAGTATGTCACAAGACCAATCTAATAAAGTAGTAACAAATGA
TATCAACCCTATTTCAATGCATCAAGATGTCATAAATCAATATGCACAAATAGATGAATTAAATCCTTTTAATGTACCAT
CACCTCATATGACAATCCAACATGTTAAACAATTAAAGGATATTATTTTAGAAGTAGTAACAAATAAATATTATGATGGT
TTCGTTATCACGCATGGTACCGATACGTTAGAAGAAACTGCCTTTTTACTTGATTTAATATTAGGTATCGAGCAACCTGT
TGTTATTACTGGCGCAATGCGCTCGTCTAATGAAATTGGTTCTGACGGATTATATAATTATATTTCCGCTATTCGCGTTG
CCTCTGATGAAAAGGCCCGTCATAAAGGCGTGATAGTTGTATTTAATGATGAAATACATACGGCCCGTAATGTTACCAAA
ACACATACGTCTAATACAAACACATTTCAAAGTCCAAATCATGGTCCGCTAGGTGTATTGACAAAGGATCGTGTGCAATT
CCATCATATGCCATATCGCCAACAAGCATTGGAAACTGTCAATGAGAAACTAAATGTACCATTAGTAAAAGCATATATGG
GTATGCCAGGTGACATTTTTAGTTTTTATAGTCGTGAAGGTATCGATGGTATGGTTATTGAAGCGTTAGGACAAGGCAAC
ATACCTCCAAGCGCATTAGAAGGCATTCAACAATTAGTATCTTTAAATATACCTATTGTGCTAGTTTCACGTTCCTTTAA
TGGTATTGTGAGCCCAACTTACGCATACGATGGTGGTGGTTACCAACTCGCACAACAAGGTTTTATTTTTTCTAACGGTT
TGAATGGTCCAAAAGCAAGATTAAAATTATTAGTCGCGTTAAGCAACAATTTAGATAAAACTGAAATCAAATCATATTTT
GAATTATAA

Upstream 100 bases:

>100_bases
TAATGCAATATTAATGGCTTTCATGCGTCATTTCCCTTCCTTGTCGTTTTATGAATAATATTTTATCATAATTAGGTAAA
TGAAATATAGGAGGAAATAT

Downstream 100 bases:

>100_bases
TGATTACGTAACATAAAAAGGTCTATGTACAATAATTAGTCATCAACCTCATTTGACATATCATGAATGTCAGTCGTTGT
AAAACTAAGTTATTTAGTAC

Product: L-asparaginase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MKHLLVIHTGGTISMSQDQSNKVVTNDINPISMHQDVINQYAQIDELNPFNVPSPHMTIQHVKQLKDIILEVVTNKYYDG
FVITHGTDTLEETAFLLDLILGIEQPVVITGAMRSSNEIGSDGLYNYISAIRVASDEKARHKGVIVVFNDEIHTARNVTK
THTSNTNTFQSPNHGPLGVLTKDRVQFHHMPYRQQALETVNEKLNVPLVKAYMGMPGDIFSFYSREGIDGMVIEALGQGN
IPPSALEGIQQLVSLNIPIVLVSRSFNGIVSPTYAYDGGGYQLAQQGFIFSNGLNGPKARLKLLVALSNNLDKTEIKSYF
EL

Sequences:

>Translated_322_residues
MKHLLVIHTGGTISMSQDQSNKVVTNDINPISMHQDVINQYAQIDELNPFNVPSPHMTIQHVKQLKDIILEVVTNKYYDG
FVITHGTDTLEETAFLLDLILGIEQPVVITGAMRSSNEIGSDGLYNYISAIRVASDEKARHKGVIVVFNDEIHTARNVTK
THTSNTNTFQSPNHGPLGVLTKDRVQFHHMPYRQQALETVNEKLNVPLVKAYMGMPGDIFSFYSREGIDGMVIEALGQGN
IPPSALEGIQQLVSLNIPIVLVSRSFNGIVSPTYAYDGGGYQLAQQGFIFSNGLNGPKARLKLLVALSNNLDKTEIKSYF
EL
>Mature_322_residues
MKHLLVIHTGGTISMSQDQSNKVVTNDINPISMHQDVINQYAQIDELNPFNVPSPHMTIQHVKQLKDIILEVVTNKYYDG
FVITHGTDTLEETAFLLDLILGIEQPVVITGAMRSSNEIGSDGLYNYISAIRVASDEKARHKGVIVVFNDEIHTARNVTK
THTSNTNTFQSPNHGPLGVLTKDRVQFHHMPYRQQALETVNEKLNVPLVKAYMGMPGDIFSFYSREGIDGMVIEALGQGN
IPPSALEGIQQLVSLNIPIVLVSRSFNGIVSPTYAYDGGGYQLAQQGFIFSNGLNGPKARLKLLVALSNNLDKTEIKSYF
EL

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=304, Percent_Identity=23.6842105263158, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1789327, Length=248, Percent_Identity=35.4838709677419, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1788065, Length=341, Percent_Identity=28.7390029325513, Blast_Score=93, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17558278, Length=344, Percent_Identity=24.7093023255814, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI193207372, Length=344, Percent_Identity=24.7093023255814, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI193207374, Length=344, Percent_Identity=24.7093023255814, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17558280, Length=344, Percent_Identity=24.7093023255814, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323189, Length=338, Percent_Identity=29.8816568047337, Blast_Score=128, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6323187, Length=338, Percent_Identity=29.8816568047337, Blast_Score=128, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6323186, Length=338, Percent_Identity=29.8816568047337, Blast_Score=128, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6323184, Length=338, Percent_Identity=29.8816568047337, Blast_Score=128, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6320527, Length=252, Percent_Identity=30.5555555555556, Blast_Score=117, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24645530, Length=325, Percent_Identity=27.6923076923077, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24639609, Length=240, Percent_Identity=27.0833333333333, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 35620; Mature: 35620

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHLLVIHTGGTISMSQDQSNKVVTNDINPISMHQDVINQYAQIDELNPFNVPSPHMTIQ
CCEEEEEECCCEEEECCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
HVKQLKDIILEVVTNKYYDGFVITHGTDTLEETAFLLDLILGIEQPVVITGAMRSSNEIG
HHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCC
SDGLYNYISAIRVASDEKARHKGVIVVFNDEIHTARNVTKTHTSNTNTFQSPNHGPLGVL
CCHHHHHHHHHHHCCCCHHHHCCEEEEECCCCHHHHCCHHCCCCCCCCCCCCCCCCCCCE
TKDRVQFHHMPYRQQALETVNEKLNVPLVKAYMGMPGDIFSFYSREGIDGMVIEALGQGN
ECCCHHHHCCCHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHCCCCEEEEEECCCCC
IPPSALEGIQQLVSLNIPIVLVSRSFNGIVSPTYAYDGGGYQLAQQGFIFSNGLNGPKAR
CCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCHHHHHCCEEEECCCCCHHHH
LKLLVALSNNLDKTEIKSYFEL
HEEEEECCCCCCHHHHHHHHCC
>Mature Secondary Structure
MKHLLVIHTGGTISMSQDQSNKVVTNDINPISMHQDVINQYAQIDELNPFNVPSPHMTIQ
CCEEEEEECCCEEEECCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
HVKQLKDIILEVVTNKYYDGFVITHGTDTLEETAFLLDLILGIEQPVVITGAMRSSNEIG
HHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCC
SDGLYNYISAIRVASDEKARHKGVIVVFNDEIHTARNVTKTHTSNTNTFQSPNHGPLGVL
CCHHHHHHHHHHHCCCCHHHHCCEEEEECCCCHHHHCCHHCCCCCCCCCCCCCCCCCCCE
TKDRVQFHHMPYRQQALETVNEKLNVPLVKAYMGMPGDIFSFYSREGIDGMVIEALGQGN
ECCCHHHHCCCHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHCCCCEEEEEECCCCC
IPPSALEGIQQLVSLNIPIVLVSRSFNGIVSPTYAYDGGGYQLAQQGFIFSNGLNGPKAR
CCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCHHHHHCCEEEECCCCCHHHH
LKLLVALSNNLDKTEIKSYFEL
HEEEEECCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1916233 [H]