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The map label for this gene is grlB

Identifier: 82750947

GI number: 82750947

Start: 1319966

End: 1321963

Strand: Direct

Name: grlB

Synonym: SAB1211

Alternate gene names: 82750947

Gene position: 1319966-1321963 (Clockwise)

Preceding gene: 82750944

Following gene: 82750948

Centisome position: 48.13

GC content: 34.78

Gene sequence:

>1998_bases
TTGGCAATGAATAAACAAAATAATTATTCAGATGATTCAATACAGGTTTTAGAGGGGTTAGAAGCAGTTCGTAAAAGACC
TGGTATGTATATTGGATCAACTGATAAACGGGGATTACATCATCTAGTATATGAAATTGTCGATAACTCCGTCGATGAAG
TATTGAATGGTTACGGTAACGAAATAGATGTAACAATTAATAAAGATGGTAGTATTTCTATAGAAGATAATGGACGTGGT
ATGCCAACAGGTATACATAAATCAGGTAAACCGACAGTCGAAGTTATCTTTACTGTTTTACATGCAGGAGGTAAATTTGG
ACAAAGCGGCTATAAAACTTCAGGTGGTCTTCACGGTGTTGGTGCTTCAGTTGTAAATGCATTGAGTGAATGGCTTGAAG
TTGAAATCCATCGAGATGGTAATATATATCATCAAAGTTTTAAAAACGGTGGTTCGCCATCTTCTGGTTTAGTAAAAAAA
GGTAAAACTAAGAAAACAGGTACCAAAGTAACATTTAAACCTGATGACACAATTTTTAAAGCATCTACATCATTTAATTT
TGATGTTTTAAGTGAACGACTACAAGAGTCTGCGTTCTTATTGAAAAATTTAAAAATAACGCTTAATGATTTACGCAGTG
GTAAAGAGCGTCAAGAGCATTACCATTATGAAGAAGGAATCAAAGAATTTGTTGGTTATGTCAATGAAGGAAAAGAAGTT
TTGCATGACGTGGCTACATTTTCAGGTGAAGCAAATGGTATAGAGGTAGATGTAGCTTTCCAATATAATGATCAATATTC
AGAAAGTATTTTAAGTTTCGTAAATAATGTACGTACTAAAGATGGTGGTACACATGAAGTTGGTTTTAAAACAGCAATGA
CACGTGTATTTAATGATTATGCACGTCGTATTAATGAACTTAAAACAAAAGATAAAAACTTAGACGGTAATGATATTCGT
GAAGGTTTAACAGCTGTTGTGTCTATACGTATTCCAGAAGAATTACTACAATTTGAAGGACAAACGAAATCTAAATTGGG
TACTTCTGAAGCAAGAAGTGCTGTTGATTCAGTTGTTGCAGACAAATTGCCATTCTATTTAGAAGAAAAAGGACAATTGT
CTAAATCACTTGTGAAAAAAGCGATTAAAGCACAACAAGCAAGGGAAGCTGCACGTAAAGCTCGTGAAGATGCTCGTTCA
GGTAAGAAAAACAAGCGTAAAGACACTTTGCTATCTGGTAAATTAACACCTGCACAAAGTAAAAATACAGATAAAAATGA
ATTGTATTTAGTCGAAGGTGATTCTGCGGGAGGTTCAGCAAAACTTGGACGAGACCGCAAATTCCAAGCGATATTACCAT
TACGTGGTAAGGTAATTAATACAGAGAAAGCACGTCTGGAAGATATTTTTAAAAATGAAGAAATTAATACAATTATCCAC
ACAATCGGGGCAGGCGTTGGTACTGACTTTAAAATTGAAGATAGTAACTATAATCGTGTAATTATTATGACTGATGCTGA
TACTGATGGTGCGCATATTCAAGTGCTATTGTTAACATTCTTCTTCAAATATATGAAACCGCTTGTTCAAGCAGGTCGTG
TATTTATTGCTTTACCTCCACTTTATAAATTGGAAAAAGGTAAAGGCAAAACAAAGCGAGTTGAATACGCTTGGACAGAC
GAAGAGCTTAATAAATTACAAAAAGAACTTGGTAAAGGCTTCACGTTACAACGTTACAAAGGTTTGGGTGAAATGAACCC
TGAACAATTATGGGAAACGACGATGAACCCAGAAACACGAACTTTAATTCGTGTACAAGTTGAAGATGAATTGCGTTCAT
CTAAACGTGTAACAACATTAATGGGTGACAAAGTACAACCTAGACGTGAATGGATTGAAAAGCATGTTGAGTTTGGTATG
CAAGAGGACCAAAGTATTTTAGATAATTCTGAAGTACAAGTGCTTGAAAATGATCAATTTGATGAGGAGGAAATCTAG

Upstream 100 bases:

>100_bases
CTATGAAAGTTAATTGATACTTGCATTTTACGCTGATTTATATAAGAATAACTATTGTATAGTTTTAAAAACGAACGTAC
GTTTGCAGGAGGCGAAATCA

Downstream 100 bases:

>100_bases
TGAGTGAAATAATTCAAGATTTATCACTTGAAGATGTTTTAGGTGATCGCTTTGGAAGATATAGTAAATATATTATTCAA
GAGCGTGCATTGCCAGATGT

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase IV subunit B [H]

Number of amino acids: Translated: 665; Mature: 664

Protein sequence:

>665_residues
MAMNKQNNYSDDSIQVLEGLEAVRKRPGMYIGSTDKRGLHHLVYEIVDNSVDEVLNGYGNEIDVTINKDGSISIEDNGRG
MPTGIHKSGKPTVEVIFTVLHAGGKFGQSGYKTSGGLHGVGASVVNALSEWLEVEIHRDGNIYHQSFKNGGSPSSGLVKK
GKTKKTGTKVTFKPDDTIFKASTSFNFDVLSERLQESAFLLKNLKITLNDLRSGKERQEHYHYEEGIKEFVGYVNEGKEV
LHDVATFSGEANGIEVDVAFQYNDQYSESILSFVNNVRTKDGGTHEVGFKTAMTRVFNDYARRINELKTKDKNLDGNDIR
EGLTAVVSIRIPEELLQFEGQTKSKLGTSEARSAVDSVVADKLPFYLEEKGQLSKSLVKKAIKAQQAREAARKAREDARS
GKKNKRKDTLLSGKLTPAQSKNTDKNELYLVEGDSAGGSAKLGRDRKFQAILPLRGKVINTEKARLEDIFKNEEINTIIH
TIGAGVGTDFKIEDSNYNRVIIMTDADTDGAHIQVLLLTFFFKYMKPLVQAGRVFIALPPLYKLEKGKGKTKRVEYAWTD
EELNKLQKELGKGFTLQRYKGLGEMNPEQLWETTMNPETRTLIRVQVEDELRSSKRVTTLMGDKVQPRREWIEKHVEFGM
QEDQSILDNSEVQVLENDQFDEEEI

Sequences:

>Translated_665_residues
MAMNKQNNYSDDSIQVLEGLEAVRKRPGMYIGSTDKRGLHHLVYEIVDNSVDEVLNGYGNEIDVTINKDGSISIEDNGRG
MPTGIHKSGKPTVEVIFTVLHAGGKFGQSGYKTSGGLHGVGASVVNALSEWLEVEIHRDGNIYHQSFKNGGSPSSGLVKK
GKTKKTGTKVTFKPDDTIFKASTSFNFDVLSERLQESAFLLKNLKITLNDLRSGKERQEHYHYEEGIKEFVGYVNEGKEV
LHDVATFSGEANGIEVDVAFQYNDQYSESILSFVNNVRTKDGGTHEVGFKTAMTRVFNDYARRINELKTKDKNLDGNDIR
EGLTAVVSIRIPEELLQFEGQTKSKLGTSEARSAVDSVVADKLPFYLEEKGQLSKSLVKKAIKAQQAREAARKAREDARS
GKKNKRKDTLLSGKLTPAQSKNTDKNELYLVEGDSAGGSAKLGRDRKFQAILPLRGKVINTEKARLEDIFKNEEINTIIH
TIGAGVGTDFKIEDSNYNRVIIMTDADTDGAHIQVLLLTFFFKYMKPLVQAGRVFIALPPLYKLEKGKGKTKRVEYAWTD
EELNKLQKELGKGFTLQRYKGLGEMNPEQLWETTMNPETRTLIRVQVEDELRSSKRVTTLMGDKVQPRREWIEKHVEFGM
QEDQSILDNSEVQVLENDQFDEEEI
>Mature_664_residues
AMNKQNNYSDDSIQVLEGLEAVRKRPGMYIGSTDKRGLHHLVYEIVDNSVDEVLNGYGNEIDVTINKDGSISIEDNGRGM
PTGIHKSGKPTVEVIFTVLHAGGKFGQSGYKTSGGLHGVGASVVNALSEWLEVEIHRDGNIYHQSFKNGGSPSSGLVKKG
KTKKTGTKVTFKPDDTIFKASTSFNFDVLSERLQESAFLLKNLKITLNDLRSGKERQEHYHYEEGIKEFVGYVNEGKEVL
HDVATFSGEANGIEVDVAFQYNDQYSESILSFVNNVRTKDGGTHEVGFKTAMTRVFNDYARRINELKTKDKNLDGNDIRE
GLTAVVSIRIPEELLQFEGQTKSKLGTSEARSAVDSVVADKLPFYLEEKGQLSKSLVKKAIKAQQAREAARKAREDARSG
KKNKRKDTLLSGKLTPAQSKNTDKNELYLVEGDSAGGSAKLGRDRKFQAILPLRGKVINTEKARLEDIFKNEEINTIIHT
IGAGVGTDFKIEDSNYNRVIIMTDADTDGAHIQVLLLTFFFKYMKPLVQAGRVFIALPPLYKLEKGKGKTKRVEYAWTDE
ELNKLQKELGKGFTLQRYKGLGEMNPEQLWETTMNPETRTLIRVQVEDELRSSKRVTTLMGDKVQPRREWIEKHVEFGMQ
EDQSILDNSEVQVLENDQFDEEEI

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=613, Percent_Identity=25.6117455138662, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI19913408, Length=681, Percent_Identity=25.1101321585903, Blast_Score=112, Evalue=9e-25,
Organism=Escherichia coli, GI48994957, Length=565, Percent_Identity=49.7345132743363, Blast_Score=502, Evalue=1e-143,
Organism=Escherichia coli, GI1789408, Length=642, Percent_Identity=37.6947040498442, Blast_Score=405, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17535065, Length=675, Percent_Identity=25.7777777777778, Blast_Score=133, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI212645845, Length=545, Percent_Identity=26.605504587156, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI212645657, Length=207, Percent_Identity=28.5024154589372, Blast_Score=75, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=630, Percent_Identity=28.0952380952381, Blast_Score=114, Evalue=4e-26,
Organism=Drosophila melanogaster, GI17136538, Length=673, Percent_Identity=25.111441307578, Blast_Score=126, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005740 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: 5.99.1.-

Molecular weight: Translated: 74581; Mature: 74450

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMNKQNNYSDDSIQVLEGLEAVRKRPGMYIGSTDKRGLHHLVYEIVDNSVDEVLNGYGN
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHCCHHHHHHCCCC
EIDVTINKDGSISIEDNGRGMPTGIHKSGKPTVEVIFTVLHAGGKFGQSGYKTSGGLHGV
EEEEEECCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCH
GASVVNALSEWLEVEIHRDGNIYHQSFKNGGSPSSGLVKKGKTKKTGTKVTFKPDDTIFK
HHHHHHHHHHHHEEEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCCEEE
ASTSFNFDVLSERLQESAFLLKNLKITLNDLRSGKERQEHYHYEEGIKEFVGYVNEGKEV
ECCCCCHHHHHHHHHHHHHHHHHHHEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LHDVATFSGEANGIEVDVAFQYNDQYSESILSFVNNVRTKDGGTHEVGFKTAMTRVFNDY
HHHHHHHCCCCCCEEEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
ARRINELKTKDKNLDGNDIREGLTAVVSIRIPEELLQFEGQTKSKLGTSEARSAVDSVVA
HHHHHHHHHHCCCCCHHHHHHHHHHEEEEECCHHHHHCCCCCHHHCCHHHHHHHHHHHHH
DKLPFYLEEKGQLSKSLVKKAIKAQQAREAARKAREDARSGKKNKRKDTLLSGKLTPAQS
HHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCC
KNTDKNELYLVEGDSAGGSAKLGRDRKFQAILPLRGKVINTEKARLEDIFKNEEINTIIH
CCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCEEECCHHHHHHHHHCCCHHHHHHH
TIGAGVGTDFKIEDSNYNRVIIMTDADTDGAHIQVLLLTFFFKYMKPLVQAGRVFIALPP
HHCCCCCCCEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCC
LYKLEKGKGKTKRVEYAWTDEELNKLQKELGKGFTLQRYKGLGEMNPEQLWETTMNPETR
CHHHCCCCCCCEEEEEEECHHHHHHHHHHHCCCCEEHHHCCCCCCCHHHHHHHCCCCCCE
TLIRVQVEDELRSSKRVTTLMGDKVQPRREWIEKHVEFGMQEDQSILDNSEVQVLENDQF
EEEEEEEHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHCCCCEEEEECCCC
DEEEI
CCCCC
>Mature Secondary Structure 
AMNKQNNYSDDSIQVLEGLEAVRKRPGMYIGSTDKRGLHHLVYEIVDNSVDEVLNGYGN
CCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHCCHHHHHHCCCC
EIDVTINKDGSISIEDNGRGMPTGIHKSGKPTVEVIFTVLHAGGKFGQSGYKTSGGLHGV
EEEEEECCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCH
GASVVNALSEWLEVEIHRDGNIYHQSFKNGGSPSSGLVKKGKTKKTGTKVTFKPDDTIFK
HHHHHHHHHHHHEEEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCCEEE
ASTSFNFDVLSERLQESAFLLKNLKITLNDLRSGKERQEHYHYEEGIKEFVGYVNEGKEV
ECCCCCHHHHHHHHHHHHHHHHHHHEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LHDVATFSGEANGIEVDVAFQYNDQYSESILSFVNNVRTKDGGTHEVGFKTAMTRVFNDY
HHHHHHHCCCCCCEEEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
ARRINELKTKDKNLDGNDIREGLTAVVSIRIPEELLQFEGQTKSKLGTSEARSAVDSVVA
HHHHHHHHHHCCCCCHHHHHHHHHHEEEEECCHHHHHCCCCCHHHCCHHHHHHHHHHHHH
DKLPFYLEEKGQLSKSLVKKAIKAQQAREAARKAREDARSGKKNKRKDTLLSGKLTPAQS
HHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCC
KNTDKNELYLVEGDSAGGSAKLGRDRKFQAILPLRGKVINTEKARLEDIFKNEEINTIIH
CCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCEEECCHHHHHHHHHCCCHHHHHHH
TIGAGVGTDFKIEDSNYNRVIIMTDADTDGAHIQVLLLTFFFKYMKPLVQAGRVFIALPP
HHCCCCCCCEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCC
LYKLEKGKGKTKRVEYAWTDEELNKLQKELGKGFTLQRYKGLGEMNPEQLWETTMNPETR
CHHHCCCCCCCEEEEEEECHHHHHHHHHHHCCCCEEHHHCCCCCCCHHHHHHHCCCCCCE
TLIRVQVEDELRSSKRVTTLMGDKVQPRREWIEKHVEFGMQEDQSILDNSEVQVLENDQF
EEEEEEEHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHCCCCEEEEECCCC
DEEEI
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA