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The map label for this gene is yclM [H]

Identifier: 82750922

GI number: 82750922

Start: 1289878

End: 1291260

Strand: Reverse

Name: yclM [H]

Synonym: SAB1185c

Alternate gene names: 82750922

Gene position: 1291260-1289878 (Counterclockwise)

Preceding gene: 82750927

Following gene: 82750920

Centisome position: 47.08

GC content: 31.31

Gene sequence:

>1383_bases
ATGAAAGTTTCCAAATTCGGTGGTAGCTCCGTCTCCAATGCTACTCAAATTAAAAAGGTTTTAAATATTGTTAATTCTGA
TCCAGAGCGAAAGATTGTTATCGTTTCTGCTCCAGGTAAAAGACATGATAATGATATTAAAACAACTGATTTATTAATCA
GATTATATGAAAAGGTCATTAATCATCTTGATTATCATGATAAAAAAAGAGAAATTATTCAGCGTTATGATGATATTGTA
AAAGAATTGCAAATGGATGAAAGTATTTTACGGACGATAGATGTGACTTTGGAACATTATATTAATCAATTAAAAAATGA
ACCGAAGAGACTATTAGATGCATTACTTTCTTGTGGTGAAGATTTTAATGCGCAATTGATAGCTTTATATAATAATAGTC
AAGGTATACCAACAAAATATATATCACCGAAGGAAGCGGGTATTTTTGTTACTGACGTCCCACAAGAAGCACAAATATTA
GATAGTGCTTACTCTGAAATAAAAAAATTAAGAGATTATGATGAGAAACTAATTATCCCAGGATTTTTTGGCGTATCTCA
TGAAGGATATATAGTTACATTTCCACGTGGCGGGTCAGATATAACTGGCGCCATCATATCAAGTGGTGTTAGAGCCACAA
TTTATGAAAATTTCACCGATGTGTCTGGTATTTATAAAGCTAATCCAAATATCATCAAAAATCCTGAATTAATCGAAGAA
ATCACTTATCGAGAAATGCGTGAATTATCTTATGCTGGTTTTAGTGTTTTCCATGATGAAGCCTTACAACCACTTTATAA
AGATCGCATCCCTGTGGTGATTAAAAATACGAACCGCCCTCAAGATAAAGGTACATTTATAGTGCATGATAGAGAAATAA
ATGCGAAAAATGTGATTAGCGGTATAAGTTGTGATAAAAATTTTACCGTTATTAATATCAAAAAGTATTTAATGAATAGA
CAAGTCGGCTTTACTAGAAAGATATTAGGTGTCTTAGAGGATAATAATATTTCATTTGATCATATGCCTTCAGGTATTGA
TACTATCAGTATCGTTATGCGTTCAAAACAAATTCAAAATAAAGAAACAAAAGTATTAAATGAAATTCGTCAAAAATGTG
ATGTTGATGAGCTAAGTATTGAGCATGATTTAGCAATTTTAATGATTGTCGGCGAAGGTATGAATAAAGTCATTGGTACT
GCAAATAAAATTACACATGCCTTAGCTGAATCAAAAATTAACTTAAAAATGATTAACCAAGGTGCTTCGGAAATCTCGAT
GATGTTTGGAATTCATGTAGATGATGCTGAAAAAGCTGTATTATCTACATATGAGTTTTGCTATCACGGGGTTGCATTAA
AAAACTTGTGTGATAAAAACTAA

Upstream 100 bases:

>100_bases
TTGTAAAGTTCGTAATATTAAAAATATAAATACATAATATCATTCTTACTTACAAAACTGAATACAATTTTCTGATTTTT
TAGAAAGGTTGGCATGCACT

Downstream 100 bases:

>100_bases
AATAAAAGCAATTCGTAACACATGTATTCAACTGCGTTATGAATTGCTTTTTCTTAATTATTTATTGTTCTCTGGATTGC
ATCCTTAATACGTTGCTCAC

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDNDIKTTDLLIRLYEKVINHLDYHDKKREIIQRYDDIV
KELQMDESILRTIDVTLEHYINQLKNEPKRLLDALLSCGEDFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQIL
DSAYSEIKKLRDYDEKLIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENFTDVSGIYKANPNIIKNPELIEE
ITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKNTNRPQDKGTFIVHDREINAKNVISGISCDKNFTVINIKKYLMNR
QVGFTRKILGVLEDNNISFDHMPSGIDTISIVMRSKQIQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGEGMNKVIGT
ANKITHALAESKINLKMINQGASEISMMFGIHVDDAEKAVLSTYEFCYHGVALKNLCDKN

Sequences:

>Translated_460_residues
MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDNDIKTTDLLIRLYEKVINHLDYHDKKREIIQRYDDIV
KELQMDESILRTIDVTLEHYINQLKNEPKRLLDALLSCGEDFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQIL
DSAYSEIKKLRDYDEKLIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENFTDVSGIYKANPNIIKNPELIEE
ITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKNTNRPQDKGTFIVHDREINAKNVISGISCDKNFTVINIKKYLMNR
QVGFTRKILGVLEDNNISFDHMPSGIDTISIVMRSKQIQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGEGMNKVIGT
ANKITHALAESKINLKMINQGASEISMMFGIHVDDAEKAVLSTYEFCYHGVALKNLCDKN
>Mature_460_residues
MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDNDIKTTDLLIRLYEKVINHLDYHDKKREIIQRYDDIV
KELQMDESILRTIDVTLEHYINQLKNEPKRLLDALLSCGEDFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQIL
DSAYSEIKKLRDYDEKLIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENFTDVSGIYKANPNIIKNPELIEE
ITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKNTNRPQDKGTFIVHDREINAKNVISGISCDKNFTVINIKKYLMNR
QVGFTRKILGVLEDNNISFDHMPSGIDTISIVMRSKQIQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGEGMNKVIGT
ANKITHALAESKINLKMINQGASEISMMFGIHVDDAEKAVLSTYEFCYHGVALKNLCDKN

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=472, Percent_Identity=26.9067796610169, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1790455, Length=458, Percent_Identity=25.3275109170306, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1790376, Length=302, Percent_Identity=25.1655629139073, Blast_Score=75, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6320893, Length=488, Percent_Identity=24.7950819672131, Blast_Score=117, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 52002; Mature: 52002

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDNDIKTTDLLIRLYEKVI
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
NHLDYHDKKREIIQRYDDIVKELQMDESILRTIDVTLEHYINQLKNEPKRLLDALLSCGE
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
DFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQILDSAYSEIKKLRDYDEKLIIP
CCCCEEEEEEECCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEC
GFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENFTDVSGIYKANPNIIKNPELIEE
CEECCCCCCEEEEECCCCCCHHHHHHHCCCEEEEHHCCCCCCCEEECCCCCCCCHHHHHH
ITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKNTNRPQDKGTFIVHDREINAKNVIS
HHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEECCCCHHHHHC
GISCDKNFTVINIKKYLMNRQVGFTRKILGVLEDNNISFDHMPSGIDTISIVMRSKQIQN
CCCCCCCEEEEEHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCC
KETKVLNEIRQKCDVDELSIEHDLAILMIVGEGMNKVIGTANKITHALAESKINLKMINQ
HHHHHHHHHHHHCCCHHHCCCCCEEEEEEECCCHHHHHCCHHHHHHHHHHCCCCEEEECC
GASEISMMFGIHVDDAEKAVLSTYEFCYHGVALKNLCDKN
CHHHEEHHHEEEECCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDNDIKTTDLLIRLYEKVI
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
NHLDYHDKKREIIQRYDDIVKELQMDESILRTIDVTLEHYINQLKNEPKRLLDALLSCGE
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
DFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQILDSAYSEIKKLRDYDEKLIIP
CCCCEEEEEEECCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEC
GFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENFTDVSGIYKANPNIIKNPELIEE
CEECCCCCCEEEEECCCCCCHHHHHHHCCCEEEEHHCCCCCCCEEECCCCCCCCHHHHHH
ITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKNTNRPQDKGTFIVHDREINAKNVIS
HHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEECCCCHHHHHC
GISCDKNFTVINIKKYLMNRQVGFTRKILGVLEDNNISFDHMPSGIDTISIVMRSKQIQN
CCCCCCCEEEEEHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCC
KETKVLNEIRQKCDVDELSIEHDLAILMIVGEGMNKVIGTANKITHALAESKINLKMINQ
HHHHHHHHHHHHCCCHHHCCCCCEEEEEEECCCHHHHHCCHHHHHHHHHHCCCCEEEECC
GASEISMMFGIHVDDAEKAVLSTYEFCYHGVALKNLCDKN
CHHHEEHHHEEEECCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]