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The map label for this gene is miaA

Identifier: 82750901

GI number: 82750901

Start: 1267309

End: 1268256

Strand: Direct

Name: miaA

Synonym: SAB1164

Alternate gene names: 82750901

Gene position: 1267309-1268256 (Clockwise)

Preceding gene: 82750900

Following gene: 82750902

Centisome position: 46.21

GC content: 32.07

Gene sequence:

>948_bases
GTGGAATTAAATATGAATAAAAATAAGCCTTTTATTGTAGTAATTGTGGGGCCAACTGCTTCAGGTAAAACAGAGCTTAG
CATCGAACTCGCGAAGCGTATCAATGGTGAAATCATAAGCGGTGACTCTATGCAAGTCTACAAACATATGAATATTGGAA
CTGCAAAAGTAACACCTGAAGAAATGGATGGTATTCCACATCATTTAATTGATATCTTGAATCCTGATGATACATTTTCA
GCATATGAATTCAAGCGATTAGCAGAAGATTTAATTACTGATATAACGAATAGAGGTAAAGTTCCAATCATAGCAGGTGG
AACAGGCTTATATATTCAATCATTAATATATAATTATGAATTAGAAGATGAAACAGTTACATCTGCACAATTATCCGTAG
TTAAACAAAAGTTATCTGCATTAGAACATTTAGATAATCAGCAACTACACGATTATTTAGCTCAATTTGACGAGGCTTCT
GCCAAAAATATTCATCCAAACAATCGACAGAGAGTATTGCGCGCTATCGAATACTATTTTAAAACAAAAAAACTTTTAAG
TAATCGTAAGAAAGTGCAACAATTTACTGAAAATTATGATACATTATTAATAGGGATTGAAATGTCGCGCAAAACATTAT
ATTCAAGAATAAATAAACGTGTTGATATTATGTTGGATCACGGATTATTTAGAGAAGTGCAACAACTTGTTGAACAAGGC
TATGAATCTTGCCGAAGTATGCAAGCTATTGGATATAAAGAATTAATACCTGTGATTAACGGACAAATGATTTATGAAGA
TGCTGTCAATGATTTAAAGCAACATTCACGCCAATATGCAAAACGACAAATGACATGGTTCAAGAATAAAATGAGTGTTC
ATTGGTTAGATAAAGAAAATATGTCACTTCAAATGATGTTAGATGAGATTACAACCCAGATTAAGTAA

Upstream 100 bases:

>100_bases
ATCATCATAAACGTCATGAAGTGTTATTTGAAAAAGATCATGACAAAATTTGGGAAGACTTGTTTAAATGGTTGAATCAA
TTTTATAAAAAATAAAGAAA

Downstream 100 bases:

>100_bases
AAGGAGTCCGACAGATGATTGCAAACGAAAACATCCAAGACAAAGCACTAGAGAATTTTAAAGCAAACCAAACTGAAGTA
ACTGTATTCTTTCTAAACGG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MELNMNKNKPFIVVIVGPTASGKTELSIELAKRINGEIISGDSMQVYKHMNIGTAKVTPEEMDGIPHHLIDILNPDDTFS
AYEFKRLAEDLITDITNRGKVPIIAGGTGLYIQSLIYNYELEDETVTSAQLSVVKQKLSALEHLDNQQLHDYLAQFDEAS
AKNIHPNNRQRVLRAIEYYFKTKKLLSNRKKVQQFTENYDTLLIGIEMSRKTLYSRINKRVDIMLDHGLFREVQQLVEQG
YESCRSMQAIGYKELIPVINGQMIYEDAVNDLKQHSRQYAKRQMTWFKNKMSVHWLDKENMSLQMMLDEITTQIK

Sequences:

>Translated_315_residues
MELNMNKNKPFIVVIVGPTASGKTELSIELAKRINGEIISGDSMQVYKHMNIGTAKVTPEEMDGIPHHLIDILNPDDTFS
AYEFKRLAEDLITDITNRGKVPIIAGGTGLYIQSLIYNYELEDETVTSAQLSVVKQKLSALEHLDNQQLHDYLAQFDEAS
AKNIHPNNRQRVLRAIEYYFKTKKLLSNRKKVQQFTENYDTLLIGIEMSRKTLYSRINKRVDIMLDHGLFREVQQLVEQG
YESCRSMQAIGYKELIPVINGQMIYEDAVNDLKQHSRQYAKRQMTWFKNKMSVHWLDKENMSLQMMLDEITTQIK
>Mature_315_residues
MELNMNKNKPFIVVIVGPTASGKTELSIELAKRINGEIISGDSMQVYKHMNIGTAKVTPEEMDGIPHHLIDILNPDDTFS
AYEFKRLAEDLITDITNRGKVPIIAGGTGLYIQSLIYNYELEDETVTSAQLSVVKQKLSALEHLDNQQLHDYLAQFDEAS
AKNIHPNNRQRVLRAIEYYFKTKKLLSNRKKVQQFTENYDTLLIGIEMSRKTLYSRINKRVDIMLDHGLFREVQQLVEQG
YESCRSMQAIGYKELIPVINGQMIYEDAVNDLKQHSRQYAKRQMTWFKNKMSVHWLDKENMSLQMMLDEITTQIK

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=310, Percent_Identity=29.6774193548387, Blast_Score=138, Evalue=8e-33,
Organism=Escherichia coli, GI1790613, Length=294, Percent_Identity=36.0544217687075, Blast_Score=184, Evalue=9e-48,
Organism=Caenorhabditis elegans, GI25144712, Length=300, Percent_Identity=33.6666666666667, Blast_Score=164, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6324848, Length=319, Percent_Identity=35.423197492163, Blast_Score=191, Evalue=1e-49,
Organism=Drosophila melanogaster, GI24649830, Length=339, Percent_Identity=32.7433628318584, Blast_Score=172, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 36423; Mature: 36423

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELNMNKNKPFIVVIVGPTASGKTELSIELAKRINGEIISGDSMQVYKHMNIGTAKVTPE
CCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCEEECCCCCEEHEECCCCCCCCCHH
EMDGIPHHLIDILNPDDTFSAYEFKRLAEDLITDITNRGKVPIIAGGTGLYIQSLIYNYE
HHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCC
LEDETVTSAQLSVVKQKLSALEHLDNQQLHDYLAQFDEASAKNIHPNNRQRVLRAIEYYF
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
KTKKLLSNRKKVQQFTENYDTLLIGIEMSRKTLYSRINKRVDIMLDHGLFREVQQLVEQG
HHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHH
YESCRSMQAIGYKELIPVINGQMIYEDAVNDLKQHSRQYAKRQMTWFKNKMSVHWLDKEN
HHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
MSLQMMLDEITTQIK
CHHHHHHHHHHHHCC
>Mature Secondary Structure
MELNMNKNKPFIVVIVGPTASGKTELSIELAKRINGEIISGDSMQVYKHMNIGTAKVTPE
CCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCEEECCCCCEEHEECCCCCCCCCHH
EMDGIPHHLIDILNPDDTFSAYEFKRLAEDLITDITNRGKVPIIAGGTGLYIQSLIYNYE
HHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCC
LEDETVTSAQLSVVKQKLSALEHLDNQQLHDYLAQFDEASAKNIHPNNRQRVLRAIEYYF
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
KTKKLLSNRKKVQQFTENYDTLLIGIEMSRKTLYSRINKRVDIMLDHGLFREVQQLVEQG
HHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHH
YESCRSMQAIGYKELIPVINGQMIYEDAVNDLKQHSRQYAKRQMTWFKNKMSVHWLDKEN
HHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
MSLQMMLDEITTQIK
CHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA