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The map label for this gene is pyrAA

Identifier: 82750807

GI number: 82750807

Start: 1149441

End: 1150541

Strand: Direct

Name: pyrAA

Synonym: SAB1066

Alternate gene names: 82750807

Gene position: 1149441-1150541 (Clockwise)

Preceding gene: 82750806

Following gene: 82750808

Centisome position: 41.91

GC content: 35.24

Gene sequence:

>1101_bases
ATGCAAAGCAAACGTTATCTAGTGTTAGAAGACGGTTCTTTTTACGAAGGCTACCGTTTAGGATCTGATAACTTAACTGT
AGGAGAAATTGTATTTAATACAGCGATGACAGGTTATCAAGAAACTATTTCAGATCTATCATATACAGGTCAGATCATTA
CTTTTACGTATCCATTAATCGGTAATTATGGTATCAATAGAGACGATTTTGAATCATTAGTACCTACATTAAACGGTATT
GTAGTGAAAGAAGCGAGTGCGCATCCAAGTAATTTTAGACAACAAAAGACACTTCATGACGTTTTAGAATTGCATCAAAT
TCCAGGGATTTCAGGTGTTGATACAAGAAGTATTACGCGTAAAATTCGACAACACGGTGTGTTAAAAGCTGGTTTTACTG
ATCGAAAAGAAGATATTGATCAACTTGTCAAACATTTACAACAAGTAGAATTACCTAAAAACGAAGTAGAAATCGTTTCG
ACTAAAACACCGTATGTTTCGACAGGTAAGGATCTAAGTGTAGTACTTGTAGACTTTGGTAAGAAGCAAAATATTGTTCG
AGAATTAAACGTCAGAGGTTGTAACGTCACAGTTGTACCATATACAACTACTGCCGAAGAAATTTTAGCAATGGCTCCAG
ATGGCGTTATGCTATCAAACGGACCAGGTAATCCTGAAGTTGTAGAATGTGCGATTCCAATGATTCAAGGAATTTTAGGG
AAAATTCCGTTCTTTGGTATCTGTCTAGGACATCAACTTTTTGCATTATCTCAAGGAGCACGCTCATTTAAAATGAAGTT
TGGTCATCGAGGTGCGAACCATCCAGTTAAAAATTTAGAGACTGGAAAAGTTGATATTACGAGTCAAAACCATGGATATG
CAATAGATATAGATTCGTTAAAAAGAACTGATTTAGAAATTACTCATCTTGCATTAAATGATGGTACTGTAGAAGGTTTA
AAACATAAAACATTACCAGCATTTTCTGTTCAATACCATCCTGAAGCAAATCCAGGACCGTCAGATTCAAACTATCTATT
TGATGACTTTGTAGCAATGATGACTAATTTTAAGGAAAAGGAGCGTCATATCAATGCCTAA

Upstream 100 bases:

>100_bases
TCAAAAGCAGATAATACACCATTTATCGGCTATAAAGTTTATGGAAATCCAATCTTAACAATGGTTGAAGGCGAAGTTAA
ATTTGAGGGGGATAAATAAT

Downstream 100 bases:

>100_bases
ACGTAATGATATCAAAACAATTTTAGTAATAGGGTCTGGGCCAATTATCATAGGTCAAGCAGCTGAATTTGATTATGCTG
GAACACAAGCATGTCTAGCT

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MQSKRYLVLEDGSFYEGYRLGSDNLTVGEIVFNTAMTGYQETISDLSYTGQIITFTYPLIGNYGINRDDFESLVPTLNGI
VVKEASAHPSNFRQQKTLHDVLELHQIPGISGVDTRSITRKIRQHGVLKAGFTDRKEDIDQLVKHLQQVELPKNEVEIVS
TKTPYVSTGKDLSVVLVDFGKKQNIVRELNVRGCNVTVVPYTTTAEEILAMAPDGVMLSNGPGNPEVVECAIPMIQGILG
KIPFFGICLGHQLFALSQGARSFKMKFGHRGANHPVKNLETGKVDITSQNHGYAIDIDSLKRTDLEITHLALNDGTVEGL
KHKTLPAFSVQYHPEANPGPSDSNYLFDDFVAMMTNFKEKERHINA

Sequences:

>Translated_366_residues
MQSKRYLVLEDGSFYEGYRLGSDNLTVGEIVFNTAMTGYQETISDLSYTGQIITFTYPLIGNYGINRDDFESLVPTLNGI
VVKEASAHPSNFRQQKTLHDVLELHQIPGISGVDTRSITRKIRQHGVLKAGFTDRKEDIDQLVKHLQQVELPKNEVEIVS
TKTPYVSTGKDLSVVLVDFGKKQNIVRELNVRGCNVTVVPYTTTAEEILAMAPDGVMLSNGPGNPEVVECAIPMIQGILG
KIPFFGICLGHQLFALSQGARSFKMKFGHRGANHPVKNLETGKVDITSQNHGYAIDIDSLKRTDLEITHLALNDGTVEGL
KHKTLPAFSVQYHPEANPGPSDSNYLFDDFVAMMTNFKEKERHINA
>Mature_366_residues
MQSKRYLVLEDGSFYEGYRLGSDNLTVGEIVFNTAMTGYQETISDLSYTGQIITFTYPLIGNYGINRDDFESLVPTLNGI
VVKEASAHPSNFRQQKTLHDVLELHQIPGISGVDTRSITRKIRQHGVLKAGFTDRKEDIDQLVKHLQQVELPKNEVEIVS
TKTPYVSTGKDLSVVLVDFGKKQNIVRELNVRGCNVTVVPYTTTAEEILAMAPDGVMLSNGPGNPEVVECAIPMIQGILG
KIPFFGICLGHQLFALSQGARSFKMKFGHRGANHPVKNLETGKVDITSQNHGYAIDIDSLKRTDLEITHLALNDGTVEGL
KHKTLPAFSVQYHPEANPGPSDSNYLFDDFVAMMTNFKEKERHINA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=358, Percent_Identity=42.1787709497207, Blast_Score=261, Evalue=1e-69,
Organism=Homo sapiens, GI21361331, Length=367, Percent_Identity=36.5122615803815, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI169790915, Length=367, Percent_Identity=36.5122615803815, Blast_Score=216, Evalue=3e-56,
Organism=Escherichia coli, GI1786215, Length=385, Percent_Identity=44.1558441558442, Blast_Score=297, Evalue=8e-82,
Organism=Caenorhabditis elegans, GI193204318, Length=362, Percent_Identity=38.6740331491713, Blast_Score=242, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6324878, Length=367, Percent_Identity=40.3269754768392, Blast_Score=249, Evalue=7e-67,
Organism=Saccharomyces cerevisiae, GI6322331, Length=394, Percent_Identity=36.8020304568528, Blast_Score=240, Evalue=2e-64,
Organism=Drosophila melanogaster, GI45555749, Length=382, Percent_Identity=39.0052356020942, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24642586, Length=382, Percent_Identity=39.0052356020942, Blast_Score=253, Evalue=2e-67,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 40579; Mature: 40579

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSKRYLVLEDGSFYEGYRLGSDNLTVGEIVFNTAMTGYQETISDLSYTGQIITFTYPLI
CCCCCEEEEECCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEHHH
GNYGINRDDFESLVPTLNGIVVKEASAHPSNFRQQKTLHDVLELHQIPGISGVDTRSITR
CCCCCCHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
KIRQHGVLKAGFTDRKEDIDQLVKHLQQVELPKNEVEIVSTKTPYVSTGKDLSVVLVDFG
HHHHCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEECCCCCEEEEEECC
KKQNIVRELNVRGCNVTVVPYTTTAEEILAMAPDGVMLSNGPGNPEVVECAIPMIQGILG
CCHHHHHHHCCCCCEEEEEECCCCHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHH
KIPFFGICLGHQLFALSQGARSFKMKFGHRGANHPVKNLETGKVDITSQNHGYAIDIDSL
CCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCC
KRTDLEITHLALNDGTVEGLKHKTLPAFSVQYHPEANPGPSDSNYLFDDFVAMMTNFKEK
CCCCEEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCHHHH
ERHINA
HHCCCC
>Mature Secondary Structure
MQSKRYLVLEDGSFYEGYRLGSDNLTVGEIVFNTAMTGYQETISDLSYTGQIITFTYPLI
CCCCCEEEEECCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEHHH
GNYGINRDDFESLVPTLNGIVVKEASAHPSNFRQQKTLHDVLELHQIPGISGVDTRSITR
CCCCCCHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
KIRQHGVLKAGFTDRKEDIDQLVKHLQQVELPKNEVEIVSTKTPYVSTGKDLSVVLVDFG
HHHHCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEECCCCCEEEEEECC
KKQNIVRELNVRGCNVTVVPYTTTAEEILAMAPDGVMLSNGPGNPEVVECAIPMIQGILG
CCHHHHHHHCCCCCEEEEEECCCCHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHH
KIPFFGICLGHQLFALSQGARSFKMKFGHRGANHPVKNLETGKVDITSQNHGYAIDIDSL
CCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCC
KRTDLEITHLALNDGTVEGLKHKTLPAFSVQYHPEANPGPSDSNYLFDDFVAMMTNFKEK
CCCCEEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCHHHH
ERHINA
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA