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The map label for this gene is mraW

Identifier: 82750786

GI number: 82750786

Start: 1123722

End: 1124657

Strand: Direct

Name: mraW

Synonym: SAB1043

Alternate gene names: 82750786

Gene position: 1123722-1124657 (Clockwise)

Preceding gene: 82750785

Following gene: 82750787

Centisome position: 40.97

GC content: 35.9

Gene sequence:

>936_bases
GTGTTTCATCATATCAGCGTTATGTTAAACGAAACCATTGATTATTTAAATGTAAAAGAAAATGGTGTGTACATTGACTG
TACGCTAGGTGGAGCGGGACATGCCCTTTATTTACTAAATCAATTAAATGACGACGGAAGATTAATAGCAATCGATCAAG
ACCAAACTGCAATTGATAATGCTAAAAAGGTATTAAAGGAACATTTGCATAAGGTGACTTTTGTTCATAGCAACTTCCGT
GAATTAACTCAAATATTAAAAGACTTAAACATTGAAAAAGTAGATGGAATTTATTACGACTTGGGTGTTTCAAGCCCACA
ACTCGACATTCCAGAACGAGGATTCAGTTATCACCATGACGCAACATTAGACATGCGTATGGACCAAACACAAGAACTAA
CAGCATATGAAATTGTTAACAATTGGTCATATGAAGCGTTAGTGAAGATTTTTTATCGCTATGGCGAGGAGAAATTTTCA
AAACAGATAGCTCGAAGAATCGAAGCACATCGCGAACAACAACCAATAACAACAACATTAGAATTAGTTGACATTATAAA
AGAAGGTATTCCTGCAAAAGCAAGAAGAAAAGGCGGACATCCTGCAAAACGAGTATTTCAAGCACTACGAATTGCAGTAA
ACGATGAATTGTCAGCTTTTGAAGATTCAATAGAACAAGCGATTGAATTAGTGAAAGTAGACGGCAGGATTTCGGTAATC
ACTTTCCATTCTTTAGAAGATCGTTTATGTAAACAGGTGTTCCAAGAATATGAAAAAGGTCCAGAGGTACCAAGAGGATT
ACCAGTTATACCAGAAGCATATACACCTAAGTTAAAGCGTATTAATCGTAAACCGATTACCGCTACAGAAGAAGATTTAG
ATGACAATAACAGAGCACGAAGCGCGAAATTACGTGTAGCTGAAATACTTAAATAA

Upstream 100 bases:

>100_bases
TAGAGAAACTTGGAATGATTTCTATGAAGAATCTGAAGAAAGTTTCGAAGATATTGCTGAAGATTTAATAGATTTTGATT
TTTAAAATGGAGGAATTGAA

Downstream 100 bases:

>100_bases
GGAGCAATTTATAATGGCTGTAGAAAAAGTGTACCAACCATATGACGAACAAGTTTATAATAGTATACCGAAGCAACAAC
CACAAACTAAGCCCGAAAAG

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLNDDGRLIAIDQDQTAIDNAKKVLKEHLHKVTFVHSNFR
ELTQILKDLNIEKVDGIYYDLGVSSPQLDIPERGFSYHHDATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFS
KQIARRIEAHREQQPITTTLELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELSAFEDSIEQAIELVKVDGRISVI
TFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTPKLKRINRKPITATEEDLDDNNRARSAKLRVAEILK

Sequences:

>Translated_311_residues
MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLNDDGRLIAIDQDQTAIDNAKKVLKEHLHKVTFVHSNFR
ELTQILKDLNIEKVDGIYYDLGVSSPQLDIPERGFSYHHDATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFS
KQIARRIEAHREQQPITTTLELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELSAFEDSIEQAIELVKVDGRISVI
TFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTPKLKRINRKPITATEEDLDDNNRARSAKLRVAEILK
>Mature_311_residues
MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLNDDGRLIAIDQDQTAIDNAKKVLKEHLHKVTFVHSNFR
ELTQILKDLNIEKVDGIYYDLGVSSPQLDIPERGFSYHHDATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFS
KQIARRIEAHREQQPITTTLELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELSAFEDSIEQAIELVKVDGRISVI
TFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTPKLKRINRKPITATEEDLDDNNRARSAKLRVAEILK

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA [H]

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family [H]

Homologues:

Organism=Homo sapiens, GI165377209, Length=341, Percent_Identity=33.4310850439883, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI165377202, Length=190, Percent_Identity=36.8421052631579, Blast_Score=112, Evalue=3e-25,
Organism=Escherichia coli, GI1786270, Length=314, Percent_Identity=41.0828025477707, Blast_Score=245, Evalue=3e-66,
Organism=Drosophila melanogaster, GI62472493, Length=327, Percent_Identity=35.1681957186544, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI28571637, Length=327, Percent_Identity=35.1681957186544, Blast_Score=156, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002903 [H]

Pfam domain/function: PF01795 Methyltransf_5 [H]

EC number: NA

Molecular weight: Translated: 35709; Mature: 35709

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLNDDGRLIAIDQDQTAIDN
CCEEHHHHHHHHHHHEEEECCCEEEEEEECCCCHHHHHHHHCCCCCCEEEECCCCHHHHH
AKKVLKEHLHKVTFVHSNFRELTQILKDLNIEKVDGIYYDLGVSSPQLDIPERGFSYHHD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCEEEECCCCCCCCCCCCCCCCCCCC
ATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFSKQIARRIEAHREQQPITTTL
CEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHH
ELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELSAFEDSIEQAIELVKVDGRISVI
HHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEE
TFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTPKLKRINRKPITATEEDLDDNNRAR
EEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHCCCCCCCHH
SAKLRVAEILK
HHHHHHHHHCC
>Mature Secondary Structure
MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLNDDGRLIAIDQDQTAIDN
CCEEHHHHHHHHHHHEEEECCCEEEEEEECCCCHHHHHHHHCCCCCCEEEECCCCHHHHH
AKKVLKEHLHKVTFVHSNFRELTQILKDLNIEKVDGIYYDLGVSSPQLDIPERGFSYHHD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCEEEECCCCCCCCCCCCCCCCCCCC
ATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFSKQIARRIEAHREQQPITTTL
CEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHH
ELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELSAFEDSIEQAIELVKVDGRISVI
HHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEE
TFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTPKLKRINRKPITATEEDLDDNNRAR
EEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHCCCCCCCHH
SAKLRVAEILK
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA