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The map label for this gene is ptsI

Identifier: 82750694

GI number: 82750694

Start: 1029931

End: 1031649

Strand: Direct

Name: ptsI

Synonym: SAB0950

Alternate gene names: 82750694

Gene position: 1029931-1031649 (Clockwise)

Preceding gene: 82750693

Following gene: 82750696

Centisome position: 37.55

GC content: 35.14

Gene sequence:

>1719_bases
ATGTCTAAATTAATTAAAGGTATTGCCGCATCTGATGGTGTCGCAATTGCTAAAGCTTATTTATTAGTTGAGCCAGACTT
AACATTCGACAAAAATGAAAAAGTCACTGATGTTGAAGGAGAAGTTGCAAAGTTCAATAGCGCTATCGAAGCTTCTAAAG
TTGAGTTAACTAAAATTAGAAATAATGCAGAGGTTCAACTAGGTGCTGATAAAGCTGCTATCTTTGATGCACATTTATTA
GTTTTAGATGACCCTGAATTAATTCAACCAATCCAAGATAAGATTAAAAATGAAAACGCTAATGCTGCTACAGCATTAAC
GGATGTAACAACACAATTTGTTACAATTTTTGAATCTATGGATAACGAATACATGAAAGAACGTGCGGCTGATATTCGCG
ATGTTTCTAAACGTGTGTTATCACATATTTTAGGTGTAGAATTACCGAATCCGAGTATGATCGATGAAAGCGTTGTTATT
GTAGGGAATGACTTAACGCCATCTGATACTGCTCAATTAAATAAAGAATTCGTACAAGGTTTTGCTACAAACATTGGCGG
AAGAACAAGTCACTCTGCGATTATGAGTCGTTCTTTAGAAATTCCAGCAATTGTTGGTACAAAATCAATTACTCAAGAAG
TTAAACAAGGCGACATGATTATCGTAGATGGATTAAATGGTGATGTAATCGTTAATCCAACTGAAGATGAGTTAATCGCT
TATCAAGATAAACGTGAGCGTTATTTTGCTGACAAGAAAGAATTACAAAAACTACGTGATGCTGATACTGTTACAGTTGA
TGGTGTTCACGCAGAGCTTGCTGCAAATATTGGTACACCTAATGATTTGCCAGGTGTTATTGAAAATGGTGCACAAGGTA
TCGGCTTATATAGAACTGAGTTTTTATATATGGGTCGTGACCAAATGCCTACAGAAGAAGAGCAATTTGAAGCTTATAAA
GAAGTATTAGAAGCAATGAACGGTAAACGTGTTGTTGTACGTACTTTAGATATAGGTGGAGATAAAGAATTATCATACTT
AAACTTGCCTGAAGAAATGAATCCATTCTTAGGTTATCGTGCGATTCGCTTATGCCTTGCGCAACAAGATATTTTCAGAC
CACAACTACGTGCATTATTACGTGCATCGGTTTATGGTAAGTTAAATATCATGTTCCCAATGGTTGCAACAATTAACGAA
TTTAGAGAAGCTAAAGCTATATTATTAGAAGAAAAAGAAAACCTTAAAAATGAAGGTCATGACATTTCAGATGATATAGA
ATTAGGAATCATGGTAGAGATACCTGCAACAGCAGCATTAGCTGATGTCTTTGCTAAAGAAGTAGATTTCTTCAGTATCG
GTACAAATGATTTAATTCAATACACATTAGCTGCTGACCGTATGTCAGAGCGTGTATCATATCTATACCAACCATATAAC
CCTTCAATCTTACGTTTAGTTAAAAAAGTTATTGAAGCGTCACATAAAGAAGGTAAATGGACAGGTATGTGTGGTGAAAT
GGCTGGAGATGAAACAGCTATTCCATTATTACTTGGTTTAGGTTTAGATGAGTTCTCTATGAGTGCAACGTCTATTCTGA
AAGCAAGAAGACAAATTAATGGTTTAAGTAAAAATGAAATGACTGAACTTGCTAACCGTGCAGTCGACTGTGCAACGCAA
GAAGAAGTTATTGAATTAGTTAACAACTACGTAAAATAA

Upstream 100 bases:

>100_bases
AAGATGCTGAAATTACAATTTATGCTGACGGTAGTGATGAATCTGACGCCATTCAAGCAATCAGTGACGTCTTATCAAAA
GAAGGATTGACTAAATAATC

Downstream 100 bases:

>100_bases
TTAATTCTTTCGAAAGACAGAATTATAAATAAACCCGAAGTATTCATTGTGAGTAATTATGAATACTTCGGGTTCTTTTT
TATGATTTATGTTAATTTCA

Product: phosphoenolpyruvate-protein phosphatase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 572; Mature: 571

Protein sequence:

>572_residues
MSKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAKFNSAIEASKVELTKIRNNAEVQLGADKAAIFDAHLL
VLDDPELIQPIQDKIKNENANAATALTDVTTQFVTIFESMDNEYMKERAADIRDVSKRVLSHILGVELPNPSMIDESVVI
VGNDLTPSDTAQLNKEFVQGFATNIGGRTSHSAIMSRSLEIPAIVGTKSITQEVKQGDMIIVDGLNGDVIVNPTEDELIA
YQDKRERYFADKKELQKLRDADTVTVDGVHAELAANIGTPNDLPGVIENGAQGIGLYRTEFLYMGRDQMPTEEEQFEAYK
EVLEAMNGKRVVVRTLDIGGDKELSYLNLPEEMNPFLGYRAIRLCLAQQDIFRPQLRALLRASVYGKLNIMFPMVATINE
FREAKAILLEEKENLKNEGHDISDDIELGIMVEIPATAALADVFAKEVDFFSIGTNDLIQYTLAADRMSERVSYLYQPYN
PSILRLVKKVIEASHKEGKWTGMCGEMAGDETAIPLLLGLGLDEFSMSATSILKARRQINGLSKNEMTELANRAVDCATQ
EEVIELVNNYVK

Sequences:

>Translated_572_residues
MSKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAKFNSAIEASKVELTKIRNNAEVQLGADKAAIFDAHLL
VLDDPELIQPIQDKIKNENANAATALTDVTTQFVTIFESMDNEYMKERAADIRDVSKRVLSHILGVELPNPSMIDESVVI
VGNDLTPSDTAQLNKEFVQGFATNIGGRTSHSAIMSRSLEIPAIVGTKSITQEVKQGDMIIVDGLNGDVIVNPTEDELIA
YQDKRERYFADKKELQKLRDADTVTVDGVHAELAANIGTPNDLPGVIENGAQGIGLYRTEFLYMGRDQMPTEEEQFEAYK
EVLEAMNGKRVVVRTLDIGGDKELSYLNLPEEMNPFLGYRAIRLCLAQQDIFRPQLRALLRASVYGKLNIMFPMVATINE
FREAKAILLEEKENLKNEGHDISDDIELGIMVEIPATAALADVFAKEVDFFSIGTNDLIQYTLAADRMSERVSYLYQPYN
PSILRLVKKVIEASHKEGKWTGMCGEMAGDETAIPLLLGLGLDEFSMSATSILKARRQINGLSKNEMTELANRAVDCATQ
EEVIELVNNYVK
>Mature_571_residues
SKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAKFNSAIEASKVELTKIRNNAEVQLGADKAAIFDAHLLV
LDDPELIQPIQDKIKNENANAATALTDVTTQFVTIFESMDNEYMKERAADIRDVSKRVLSHILGVELPNPSMIDESVVIV
GNDLTPSDTAQLNKEFVQGFATNIGGRTSHSAIMSRSLEIPAIVGTKSITQEVKQGDMIIVDGLNGDVIVNPTEDELIAY
QDKRERYFADKKELQKLRDADTVTVDGVHAELAANIGTPNDLPGVIENGAQGIGLYRTEFLYMGRDQMPTEEEQFEAYKE
VLEAMNGKRVVVRTLDIGGDKELSYLNLPEEMNPFLGYRAIRLCLAQQDIFRPQLRALLRASVYGKLNIMFPMVATINEF
REAKAILLEEKENLKNEGHDISDDIELGIMVEIPATAALADVFAKEVDFFSIGTNDLIQYTLAADRMSERVSYLYQPYNP
SILRLVKKVIEASHKEGKWTGMCGEMAGDETAIPLLLGLGLDEFSMSATSILKARRQINGLSKNEMTELANRAVDCATQE
EVIELVNNYVK

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=570, Percent_Identity=50.1754385964912, Blast_Score=557, Evalue=1e-160,
Organism=Escherichia coli, GI48994992, Length=520, Percent_Identity=38.0769230769231, Blast_Score=351, Evalue=6e-98,
Organism=Escherichia coli, GI1788726, Length=533, Percent_Identity=37.5234521575985, Blast_Score=344, Evalue=9e-96,
Organism=Escherichia coli, GI1789193, Length=581, Percent_Identity=32.5301204819277, Blast_Score=282, Evalue=4e-77,
Organism=Escherichia coli, GI1787994, Length=426, Percent_Identity=27.6995305164319, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI226510935, Length=184, Percent_Identity=28.804347826087, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 63277; Mature: 63146

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAKFNSAIEASKVELTKIR
CCHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEEECC
NNAEVQLGADKAAIFDAHLLVLDDPELIQPIQDKIKNENANAATALTDVTTQFVTIFESM
CCCEEEECCCCEEEEEEEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
DNEYMKERAADIRDVSKRVLSHILGVELPNPSMIDESVVIVGNDLTPSDTAQLNKEFVQG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
FATNIGGRTSHSAIMSRSLEIPAIVGTKSITQEVKQGDMIIVDGLNGDVIVNPTEDELIA
HHHHCCCCCHHHHHHHCCCCCCEEECCHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEE
YQDKRERYFADKKELQKLRDADTVTVDGVHAELAANIGTPNDLPGVIENGAQGIGLYRTE
EHHHHHHHCCCHHHHHHHHCCCCEEECCHHHHHHHCCCCCCCCCHHHHCCCCCCCCEEHH
FLYMGRDQMPTEEEQFEAYKEVLEAMNGKRVVVRTLDIGGDKELSYLNLPEEMNPFLGYR
EEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEECCCHHCCHHHHHH
AIRLCLAQQDIFRPQLRALLRASVYGKLNIMFPMVATINEFREAKAILLEEKENLKNEGH
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DISDDIELGIMVEIPATAALADVFAKEVDFFSIGTNDLIQYTLAADRMSERVSYLYQPYN
CCCCCCEEEEEEECCCHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCC
PSILRLVKKVIEASHKEGKWTGMCGEMAGDETAIPLLLGLGLDEFSMSATSILKARRQIN
CHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHC
GLSKNEMTELANRAVDCATQEEVIELVNNYVK
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAKFNSAIEASKVELTKIR
CHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEEECC
NNAEVQLGADKAAIFDAHLLVLDDPELIQPIQDKIKNENANAATALTDVTTQFVTIFESM
CCCEEEECCCCEEEEEEEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
DNEYMKERAADIRDVSKRVLSHILGVELPNPSMIDESVVIVGNDLTPSDTAQLNKEFVQG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
FATNIGGRTSHSAIMSRSLEIPAIVGTKSITQEVKQGDMIIVDGLNGDVIVNPTEDELIA
HHHHCCCCCHHHHHHHCCCCCCEEECCHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEE
YQDKRERYFADKKELQKLRDADTVTVDGVHAELAANIGTPNDLPGVIENGAQGIGLYRTE
EHHHHHHHCCCHHHHHHHHCCCCEEECCHHHHHHHCCCCCCCCCHHHHCCCCCCCCEEHH
FLYMGRDQMPTEEEQFEAYKEVLEAMNGKRVVVRTLDIGGDKELSYLNLPEEMNPFLGYR
EEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEECCCHHCCHHHHHH
AIRLCLAQQDIFRPQLRALLRASVYGKLNIMFPMVATINEFREAKAILLEEKENLKNEGH
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DISDDIELGIMVEIPATAALADVFAKEVDFFSIGTNDLIQYTLAADRMSERVSYLYQPYN
CCCCCCEEEEEEECCCHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCC
PSILRLVKKVIEASHKEGKWTGMCGEMAGDETAIPLLLGLGLDEFSMSATSILKARRQIN
CHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHC
GLSKNEMTELANRAVDCATQEEVIELVNNYVK
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA