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The map label for this gene is menF [H]

Identifier: 82750654

GI number: 82750654

Start: 984577

End: 985938

Strand: Direct

Name: menF [H]

Synonym: SAB0909

Alternate gene names: 82750654

Gene position: 984577-985938 (Clockwise)

Preceding gene: 82750652

Following gene: 82750655

Centisome position: 35.9

GC content: 31.57

Gene sequence:

>1362_bases
ATGGCTACGGGCGTATTAGAGGACGATATTGTCAAAGAGATATATGACAGCTCAAAGGAATGGGTTTCAGTTGAAGTGAA
ATTATCACAGTCACTAGACCCGAGCACATTATTTCATCTCACTGACAATGAGGCAGGAGATCGCTTTTATATGCGTTTGA
ATGATAATCGAACGTCATATTTTGGCTACAAAGCAATTCAATTATTCAAAAATAATTCTAAAAATAAAAAATCTATTTTT
AAAGACTGGGAAAAATTAAAACATAACATCACATTTATACATCCGCAATCTGAGAAACATCATCTTCGAGTTGTTGGAGG
ATTCCAATTTTCAAGTCATAAATCAGATGATGAATGGCGAGAGTTCGGACTAAATCATTTTGTATTACCTGAAGTTTTAA
TTTCAACTGATAATAATGGGACATTTTTAACTTATACAGTTAAAAGGGAAAGTTTTACTGTTGAGGAATTGAACGATTTA
ATGGATTTGTTCAACAATATATCGGATATAGATGTGGATGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAA
AGATGACTGGCGTCAACTTGTAGTAGAAGCAATAGAATCTATTAATAATGAAGAAAAAATTGTACTAGCACGTAGACGGT
TAATAAAGTTCGATAAAGATATCAGTATTCCATATATTCTAAAGCAAGCATATTCTAAAGAAAAAAACAGTTATATATTC
TTGTTAGAATCACAAGATTCTATATTCTTTTCACAAACACCTGAACAATTAATAAAGGTCAATAATAAAATACTATCGAC
TAAAGCTGTAGCAGGTACAATTAAACGTTCACAAGATGAGGACGAAGATACAAAAAATGTTGAAGCATTTTTAAAAGATA
ATAAAAACTTAATCGAACATCGATTTGTTGTTGACAGTATTTTACATGATATTAAACCTTATATCACTGAATTACATTAT
GATAAGATGCCTAAAATTCTAAAAAATGATCATTTATATCACTTGTACACTGAAATAAAGGCGCCACTGAAGGATGATTC
GTATATTAGTTTAATTGATAATTTACATCCAACACCTGCTTTAGGTGGCTATCCAAAAGAATTTGCGATGGATTTTATTG
AACAGAAAGAATTTGGTACACGAGGATTATATGGTGCGCCGGTTGGCTATATAGATATATATGATGATTGTGAATTTATT
GTTGCAATTCGTTCGATGCTTATTAAGAAAGCACAAGCAACTTTATTTGCAGGGTGTGGCATTGTTAAAGATTCTGATCC
AGATAGTGAATTGGCAGAAACGAACCTTAAGTTCACACCTATGATGAATGCATTAGGAGTCGATATGAATGGGAAATCAT
AA

Upstream 100 bases:

>100_bases
AAAATTGCAAGATAAGTTTAAGCAATTCTTGTAACACAATTATCCAAAAATGATACAATATAATAATGAGTGAAAAAAGA
CGAAGAAAGAAGTGAAATGG

Downstream 100 bases:

>100_bases
AGCAGCTTTAACGAAGCAAGTTTTTACATTTGCATCTGAGTTATATGCGTACGGCGTAAGGGAAGTAGTTATCAGTCCGG
GATCACGCTCAACGCCACTT

Product: menaquinone-specific isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MATGVLEDDIVKEIYDSSKEWVSVEVKLSQSLDPSTLFHLTDNEAGDRFYMRLNDNRTSYFGYKAIQLFKNNSKNKKSIF
KDWEKLKHNITFIHPQSEKHHLRVVGGFQFSSHKSDDEWREFGLNHFVLPEVLISTDNNGTFLTYTVKRESFTVEELNDL
MDLFNNISDIDVDEQIGEITRNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFDKDISIPYILKQAYSKEKNSYIF
LLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKRSQDEDEDTKNVEAFLKDNKNLIEHRFVVDSILHDIKPYITELHY
DKMPKILKNDHLYHLYTEIKAPLKDDSYISLIDNLHPTPALGGYPKEFAMDFIEQKEFGTRGLYGAPVGYIDIYDDCEFI
VAIRSMLIKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMNGKS

Sequences:

>Translated_453_residues
MATGVLEDDIVKEIYDSSKEWVSVEVKLSQSLDPSTLFHLTDNEAGDRFYMRLNDNRTSYFGYKAIQLFKNNSKNKKSIF
KDWEKLKHNITFIHPQSEKHHLRVVGGFQFSSHKSDDEWREFGLNHFVLPEVLISTDNNGTFLTYTVKRESFTVEELNDL
MDLFNNISDIDVDEQIGEITRNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFDKDISIPYILKQAYSKEKNSYIF
LLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKRSQDEDEDTKNVEAFLKDNKNLIEHRFVVDSILHDIKPYITELHY
DKMPKILKNDHLYHLYTEIKAPLKDDSYISLIDNLHPTPALGGYPKEFAMDFIEQKEFGTRGLYGAPVGYIDIYDDCEFI
VAIRSMLIKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMNGKS
>Mature_452_residues
ATGVLEDDIVKEIYDSSKEWVSVEVKLSQSLDPSTLFHLTDNEAGDRFYMRLNDNRTSYFGYKAIQLFKNNSKNKKSIFK
DWEKLKHNITFIHPQSEKHHLRVVGGFQFSSHKSDDEWREFGLNHFVLPEVLISTDNNGTFLTYTVKRESFTVEELNDLM
DLFNNISDIDVDEQIGEITRNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFDKDISIPYILKQAYSKEKNSYIFL
LESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKRSQDEDEDTKNVEAFLKDNKNLIEHRFVVDSILHDIKPYITELHYD
KMPKILKNDHLYHLYTEIKAPLKDDSYISLIDNLHPTPALGGYPKEFAMDFIEQKEFGTRGLYGAPVGYIDIYDDCEFIV
AIRSMLIKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMNGKS

Specific function: Siderophore biosynthesis; enterobactin biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786809, Length=303, Percent_Identity=29.042904290429, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI87082077, Length=351, Percent_Identity=26.2108262108262, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1787518, Length=258, Percent_Identity=28.6821705426357, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788114, Length=199, Percent_Identity=28.1407035175879, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320935, Length=219, Percent_Identity=30.1369863013699, Blast_Score=85, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 52369; Mature: 52238

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATGVLEDDIVKEIYDSSKEWVSVEVKLSQSLDPSTLFHLTDNEAGDRFYMRLNDNRTSY
CCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEEEECCCCCCEEEEEECCCCCEE
FGYKAIQLFKNNSKNKKSIFKDWEKLKHNITFIHPQSEKHHLRVVGGFQFSSHKSDDEWR
HHHHHHHEECCCCCCHHHHHHHHHHHHCCEEEECCCCCCCEEEEEEEEEECCCCCCHHHH
EFGLNHFVLPEVLISTDNNGTFLTYTVKRESFTVEELNDLMDLFNNISDIDVDEQIGEIT
HHCCCCEECCEEEEEECCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHH
RNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFDKDISIPYILKQAYSKEKNSYIF
CCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEE
LLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKRSQDEDEDTKNVEAFLKDNKNLIEH
EEECCCCEEECCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHH
RFVVDSILHDIKPYITELHYDKMPKILKNDHLYHLYTEIKAPLKDDSYISLIDNLHPTPA
HHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCEEEEEEHHCCCCCCCCHHHHHHCCCCCCC
LGGYPKEFAMDFIEQKEFGTRGLYGAPVGYIDIYDDCEFIVAIRSMLIKKAQATLFAGCG
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCC
IVKDSDPDSELAETNLKFTPMMNALGVDMNGKS
CCCCCCCCHHHHHCCCEEEECHHHHCCCCCCCC
>Mature Secondary Structure 
ATGVLEDDIVKEIYDSSKEWVSVEVKLSQSLDPSTLFHLTDNEAGDRFYMRLNDNRTSY
CCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEEEECCCCCCEEEEEECCCCCEE
FGYKAIQLFKNNSKNKKSIFKDWEKLKHNITFIHPQSEKHHLRVVGGFQFSSHKSDDEWR
HHHHHHHEECCCCCCHHHHHHHHHHHHCCEEEECCCCCCCEEEEEEEEEECCCCCCHHHH
EFGLNHFVLPEVLISTDNNGTFLTYTVKRESFTVEELNDLMDLFNNISDIDVDEQIGEIT
HHCCCCEECCEEEEEECCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHH
RNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFDKDISIPYILKQAYSKEKNSYIF
CCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEE
LLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKRSQDEDEDTKNVEAFLKDNKNLIEH
EEECCCCEEECCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHH
RFVVDSILHDIKPYITELHYDKMPKILKNDHLYHLYTEIKAPLKDDSYISLIDNLHPTPA
HHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCEEEEEEHHCCCCCCCCHHHHHHCCCCCCC
LGGYPKEFAMDFIEQKEFGTRGLYGAPVGYIDIYDDCEFIVAIRSMLIKKAQATLFAGCG
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCC
IVKDSDPDSELAETNLKFTPMMNALGVDMNGKS
CCCCCCCCHHHHHCCCEEEECHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629163; 8566759; 9387221; 9384377; 3131310 [H]