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The map label for this gene is yfkN [H]

Identifier: 82750636

GI number: 82750636

Start: 967724

End: 969238

Strand: Direct

Name: yfkN [H]

Synonym: SAB0890

Alternate gene names: 82750636

Gene position: 967724-969238 (Clockwise)

Preceding gene: 82750635

Following gene: 82750638

Centisome position: 35.29

GC content: 32.21

Gene sequence:

>1515_bases
ATGATGGAAAAAAATGAAAACATTAATGTAGAGATTTTAACTACGTCAGATATGCATAGTCATTTCTTAAATGGTGATTA
TGGTTCAAATATTTATAGAGCTGGTACTTATGTTAACCAAGTAAGAGCACAAAATCATCGCGTCATTTTATTAGATAGTG
GCGGAAGTTTAGCTGGCTCGTTAGCGGCCTATTATTATGCTATTGTTGCACCTTATAAACGACATCCAATGATAAAGTTA
ATGAACAGAATGCATTATGATGCTAGCGGTGTGAGTCCAAGTGAATTCAAGTTTGGTTTATCATTTTTAACTCGTTCAAT
TGCTTTGGCACGTTTTCCATGGTTATCAGCAAATATTGAATACAATGTTACTAAGGAGCCATATTTTTCAACTCCATATT
GTATTAAACATTTTGGTGACTTAAAAATTGCTATCGTAGGCGTCACAGCAGATGGTTTAATGGAAAATGAGTATTCTGAA
ATGGAGCAAGATGTATCTATTGAAAAGACATTAGTGGCCTCAAAACGATGGATTAGATATATCCATGAAGTTGAAGAGCC
AGATTCTTTGATTGTAATTTATCATGGTGGATTGAATAAAATTAGTAATAGCACGAAAAATAAAAAGGCAAGTTCGAATG
AAGCTGAAAAATTAATGGAAGAACTCGGTGTTATAGATTTAATGATTACAGCTCATCAGCATCAAACAATAGTAGGTCAA
GATCATGAAACGTATTATGTTCAGGCTGGTCAAGATGCAAAAGAGCTTGTACATCTTTCGATTAATTTTAAAAAGAGAAC
AACAACTTATGATGTTGAAAGTATTGATTCTAAAGTGATTGACTTAAATGAGTATGAAGAGGATCAAGAATTATTAGATT
TAACATTCTATGATAGAAAAGCAGTGGCTTATTGGTCACAGGAAATCATAAGTGATAAAGGTTTGATGTTATCAGTAAAT
GGGTTACAAGATTTAGTCTGTCAAACACATCCATTTTCGCAATTATTACATGATGCAATTCACCTTGCATTTGATAATGA
TATAACATGTGTCCACGTGCCTATGAACGGAGAGAAGGGGTTGAGTGGACAGATTCGAAATGAAGATTTGTATCATGCAT
ACCCATATCCAGATAAGCCAATGGATATGACAATTAGTGGTCAAAATATCAAAGATATATTGGAGTATAGTTATTCACAT
TTAGATTTTGTTAACGAGCAATTAAGCTTAACAATTATTGATGAAACATTATGTACAATGTGGCAAGGATTCAATTATGA
AATTGATATGAATCAAGAACCTGGGCAACGAGTAATGTTAGATCAAATTGATTTGACTAAGAGTTATAGAGTTACAATGA
CTGACTATTGTTATCGTAACTACAAGAATTATTTGAAAAATGCTATTATACATGAATCATACGATGAAACAATGAGTACA
TTAATTGCAGAGAAGTTAAGAGATCCGAATTATCGTATTTCATGTAGTGATAATTTTGTAGTTAAAAACAGGTAA

Upstream 100 bases:

>100_bases
TAACTGGAACTTAGATTGCTTTTTTAGTTTGTATTTTTAACTTATTTTATAAGACGATTGGTTTTGAAAATGGTAAACTA
GTAACAATGAGAGGTGTAAC

Downstream 100 bases:

>100_bases
ATATTACTATGTAATAAATTAATTATAAGTTTAACAAGTATAAAAACCTTTCTTAGAGAAGATTATCTAGGAAAGGTTTT
TAGAATTTTAAAATTGATAT

Product: 5' nucleotidase

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MMEKNENINVEILTTSDMHSHFLNGDYGSNIYRAGTYVNQVRAQNHRVILLDSGGSLAGSLAAYYYAIVAPYKRHPMIKL
MNRMHYDASGVSPSEFKFGLSFLTRSIALARFPWLSANIEYNVTKEPYFSTPYCIKHFGDLKIAIVGVTADGLMENEYSE
MEQDVSIEKTLVASKRWIRYIHEVEEPDSLIVIYHGGLNKISNSTKNKKASSNEAEKLMEELGVIDLMITAHQHQTIVGQ
DHETYYVQAGQDAKELVHLSINFKKRTTTYDVESIDSKVIDLNEYEEDQELLDLTFYDRKAVAYWSQEIISDKGLMLSVN
GLQDLVCQTHPFSQLLHDAIHLAFDNDITCVHVPMNGEKGLSGQIRNEDLYHAYPYPDKPMDMTISGQNIKDILEYSYSH
LDFVNEQLSLTIIDETLCTMWQGFNYEIDMNQEPGQRVMLDQIDLTKSYRVTMTDYCYRNYKNYLKNAIIHESYDETMST
LIAEKLRDPNYRISCSDNFVVKNR

Sequences:

>Translated_504_residues
MMEKNENINVEILTTSDMHSHFLNGDYGSNIYRAGTYVNQVRAQNHRVILLDSGGSLAGSLAAYYYAIVAPYKRHPMIKL
MNRMHYDASGVSPSEFKFGLSFLTRSIALARFPWLSANIEYNVTKEPYFSTPYCIKHFGDLKIAIVGVTADGLMENEYSE
MEQDVSIEKTLVASKRWIRYIHEVEEPDSLIVIYHGGLNKISNSTKNKKASSNEAEKLMEELGVIDLMITAHQHQTIVGQ
DHETYYVQAGQDAKELVHLSINFKKRTTTYDVESIDSKVIDLNEYEEDQELLDLTFYDRKAVAYWSQEIISDKGLMLSVN
GLQDLVCQTHPFSQLLHDAIHLAFDNDITCVHVPMNGEKGLSGQIRNEDLYHAYPYPDKPMDMTISGQNIKDILEYSYSH
LDFVNEQLSLTIIDETLCTMWQGFNYEIDMNQEPGQRVMLDQIDLTKSYRVTMTDYCYRNYKNYLKNAIIHESYDETMST
LIAEKLRDPNYRISCSDNFVVKNR
>Mature_504_residues
MMEKNENINVEILTTSDMHSHFLNGDYGSNIYRAGTYVNQVRAQNHRVILLDSGGSLAGSLAAYYYAIVAPYKRHPMIKL
MNRMHYDASGVSPSEFKFGLSFLTRSIALARFPWLSANIEYNVTKEPYFSTPYCIKHFGDLKIAIVGVTADGLMENEYSE
MEQDVSIEKTLVASKRWIRYIHEVEEPDSLIVIYHGGLNKISNSTKNKKASSNEAEKLMEELGVIDLMITAHQHQTIVGQ
DHETYYVQAGQDAKELVHLSINFKKRTTTYDVESIDSKVIDLNEYEEDQELLDLTFYDRKAVAYWSQEIISDKGLMLSVN
GLQDLVCQTHPFSQLLHDAIHLAFDNDITCVHVPMNGEKGLSGQIRNEDLYHAYPYPDKPMDMTISGQNIKDILEYSYSH
LDFVNEQLSLTIIDETLCTMWQGFNYEIDMNQEPGQRVMLDQIDLTKSYRVTMTDYCYRNYKNYLKNAIIHESYDETMST
LIAEKLRDPNYRISCSDNFVVKNR

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Escherichia coli, GI1790658, Length=252, Percent_Identity=24.6031746031746, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1786687, Length=200, Percent_Identity=30.5, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 57985; Mature: 57985

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMEKNENINVEILTTSDMHSHFLNGDYGSNIYRAGTYVNQVRAQNHRVILLDSGGSLAGS
CCCCCCCCEEEEEEECCHHHHHCCCCCCCCEEECCHHHHHHHCCCCEEEEEECCCCCHHH
LAAYYYAIVAPYKRHPMIKLMNRMHYDASGVSPSEFKFGLSFLTRSIALARFPWLSANIE
HHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEECCEE
YNVTKEPYFSTPYCIKHFGDLKIAIVGVTADGLMENEYSEMEQDVSIEKTLVASKRWIRY
EEECCCCCCCCCHHHHHCCCEEEEEEEEECCCHHCCHHHHHHHCCCHHHHHHHHHHHHHH
IHEVEEPDSLIVIYHGGLNKISNSTKNKKASSNEAEKLMEELGVIDLMITAHQHQTIVGQ
HHHCCCCCCEEEEEECCCHHHCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCEEECC
DHETYYVQAGQDAKELVHLSINFKKRTTTYDVESIDSKVIDLNEYEEDQELLDLTFYDRK
CCCEEEEECCCCHHHEEEEEEEEEECCEEEEHHHCCCEEEECCCCCCCHHHEEEEEECCH
AVAYWSQEIISDKGLMLSVNGLQDLVCQTHPFSQLLHDAIHLAFDNDITCVHVPMNGEKG
HHHHHHHHHHCCCCEEEEECCHHHHHHCCCCHHHHHHHHHEEEECCCEEEEEECCCCCCC
LSGQIRNEDLYHAYPYPDKPMDMTISGQNIKDILEYSYSHLDFVNEQLSLTIIDETLCTM
CCCCCCCCCCEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHH
WQGFNYEIDMNQEPGQRVMLDQIDLTKSYRVTMTDYCYRNYKNYLKNAIIHESYDETMST
HCCCCEEEECCCCCCCEEEEEHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LIAEKLRDPNYRISCSDNFVVKNR
HHHHHHCCCCEEEEECCCEEEECC
>Mature Secondary Structure
MMEKNENINVEILTTSDMHSHFLNGDYGSNIYRAGTYVNQVRAQNHRVILLDSGGSLAGS
CCCCCCCCEEEEEEECCHHHHHCCCCCCCCEEECCHHHHHHHCCCCEEEEEECCCCCHHH
LAAYYYAIVAPYKRHPMIKLMNRMHYDASGVSPSEFKFGLSFLTRSIALARFPWLSANIE
HHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEECCEE
YNVTKEPYFSTPYCIKHFGDLKIAIVGVTADGLMENEYSEMEQDVSIEKTLVASKRWIRY
EEECCCCCCCCCHHHHHCCCEEEEEEEEECCCHHCCHHHHHHHCCCHHHHHHHHHHHHHH
IHEVEEPDSLIVIYHGGLNKISNSTKNKKASSNEAEKLMEELGVIDLMITAHQHQTIVGQ
HHHCCCCCCEEEEEECCCHHHCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCEEECC
DHETYYVQAGQDAKELVHLSINFKKRTTTYDVESIDSKVIDLNEYEEDQELLDLTFYDRK
CCCEEEEECCCCHHHEEEEEEEEEECCEEEEHHHCCCEEEECCCCCCCHHHEEEEEECCH
AVAYWSQEIISDKGLMLSVNGLQDLVCQTHPFSQLLHDAIHLAFDNDITCVHVPMNGEKG
HHHHHHHHHHCCCCEEEEECCHHHHHHCCCCHHHHHHHHHEEEECCCEEEEEECCCCCCC
LSGQIRNEDLYHAYPYPDKPMDMTISGQNIKDILEYSYSHLDFVNEQLSLTIIDETLCTM
CCCCCCCCCCEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHH
WQGFNYEIDMNQEPGQRVMLDQIDLTKSYRVTMTDYCYRNYKNYLKNAIIHESYDETMST
HCCCCEEEECCCCCCCEEEEEHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LIAEKLRDPNYRISCSDNFVVKNR
HHHHHHCCCCEEEEECCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]