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The map label for this gene is lipA [H]

Identifier: 82750540

GI number: 82750540

Start: 859697

End: 860614

Strand: Direct

Name: lipA [H]

Synonym: SAB0791

Alternate gene names: 82750540

Gene position: 859697-860614 (Clockwise)

Preceding gene: 82750539

Following gene: 82750541

Centisome position: 31.35

GC content: 36.06

Gene sequence:

>918_bases
ATGGCGACAAAAAACGAGGAAATATTACGTAAACCGGATTGGTTGAAAATAAAATTAAATACCAACGAAAACTATACAGG
ACTTAAGAAGATGATGAGGAAAAAAAATCTTAATACTGTATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTTGGG
GTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGAGCTTGTCGTTTTTGTGCGGTTAAGACAGGT
TTACCTAATGAACTTGATTTAAATGAGCCTGAACGTGTAGCTGAATCAGTTGAATTAATGAACTTGAAACACGTTGTTAT
CACTGCTGTTGCGCGTGATGATTTAAGAGATGCTGGTTCAAATGTTTATGCTGAGACAGTACGTAAAGTTAGAGAAAGAA
ATCCATTTACAACGATTGAAATTTTACCATCAGATATGGGCGGGGACTATGATGCGTTAGAAACATTAATGGCTTCAAGA
CCTGACATTTTAAACCATAATATTGAAACTGTTCGTCGATTAACACCGAGAGTTCGTGCGCGTGCGACTTACGACAGAAC
ATTAGAGTTTTTACGTCGTTCAAAAGAATTACAACCGGATATCCCAACTAAATCAAGTATTATGGTTGGATTAGGTGAAA
CTATAGAAGAAATTTATGAAACGATGGATGATTTACGTGCGAATGATGTAGATATTTTAACGATTGGTCAATATTTACAA
CCTTCACGTAAACATTTAAAGGTTCAAAAATATTACACGCCTTTAGAGTTTGGTAAATTAAGAAAAGTGGCAATGGATAA
AGGGTTTAAACATTGCCAAGCTGGACCTTTAGTACGTAGTTCTTATCATGCGGATGAGCAAGTAAATGAAGCTGCTAAAG
AAAAGCAACGCCAAGGTGAGGCACAGTTAAATAGTTAA

Upstream 100 bases:

>100_bases
AATTATTGGAATATTAAAAAGTAAGATTATTGGATTTTCATTTGTCATGAATTTCGATATAATGTTTAAAGATACACTTA
ACAGGAGGGTATGTGTTGTT

Downstream 100 bases:

>100_bases
TATTTAACCATTAATAAGGCATAAAGGCTTAGTTTGTACAAAACGAACGTGTCATAGAAGTAATCATTATTTTTATGAAA
CACTAAAAGTATAAACTGAG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MATKNEEILRKPDWLKIKLNTNENYTGLKKMMRKKNLNTVCEEAKCPNIHECWGARRTATFMILGAVCTRACRFCAVKTG
LPNELDLNEPERVAESVELMNLKHVVITAVARDDLRDAGSNVYAETVRKVRERNPFTTIEILPSDMGGDYDALETLMASR
PDILNHNIETVRRLTPRVRARATYDRTLEFLRRSKELQPDIPTKSSIMVGLGETIEEIYETMDDLRANDVDILTIGQYLQ
PSRKHLKVQKYYTPLEFGKLRKVAMDKGFKHCQAGPLVRSSYHADEQVNEAAKEKQRQGEAQLNS

Sequences:

>Translated_305_residues
MATKNEEILRKPDWLKIKLNTNENYTGLKKMMRKKNLNTVCEEAKCPNIHECWGARRTATFMILGAVCTRACRFCAVKTG
LPNELDLNEPERVAESVELMNLKHVVITAVARDDLRDAGSNVYAETVRKVRERNPFTTIEILPSDMGGDYDALETLMASR
PDILNHNIETVRRLTPRVRARATYDRTLEFLRRSKELQPDIPTKSSIMVGLGETIEEIYETMDDLRANDVDILTIGQYLQ
PSRKHLKVQKYYTPLEFGKLRKVAMDKGFKHCQAGPLVRSSYHADEQVNEAAKEKQRQGEAQLNS
>Mature_304_residues
ATKNEEILRKPDWLKIKLNTNENYTGLKKMMRKKNLNTVCEEAKCPNIHECWGARRTATFMILGAVCTRACRFCAVKTGL
PNELDLNEPERVAESVELMNLKHVVITAVARDDLRDAGSNVYAETVRKVRERNPFTTIEILPSDMGGDYDALETLMASRP
DILNHNIETVRRLTPRVRARATYDRTLEFLRRSKELQPDIPTKSSIMVGLGETIEEIYETMDDLRANDVDILTIGQYLQP
SRKHLKVQKYYTPLEFGKLRKVAMDKGFKHCQAGPLVRSSYHADEQVNEAAKEKQRQGEAQLNS

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=299, Percent_Identity=48.1605351170569, Blast_Score=277, Evalue=8e-75,
Organism=Homo sapiens, GI37577164, Length=248, Percent_Identity=49.1935483870968, Blast_Score=242, Evalue=3e-64,
Organism=Escherichia coli, GI1786846, Length=289, Percent_Identity=52.2491349480969, Blast_Score=301, Evalue=3e-83,
Organism=Caenorhabditis elegans, GI32564533, Length=292, Percent_Identity=44.8630136986301, Blast_Score=242, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6324770, Length=297, Percent_Identity=46.1279461279461, Blast_Score=270, Evalue=2e-73,
Organism=Drosophila melanogaster, GI221513272, Length=306, Percent_Identity=47.0588235294118, Blast_Score=270, Evalue=1e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 34885; Mature: 34753

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATKNEEILRKPDWLKIKLNTNENYTGLKKMMRKKNLNTVCEEAKCPNIHECWGARRTAT
CCCCCHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHCCCCCHHHHHCCCHHHH
FMILGAVCTRACRFCAVKTGLPNELDLNEPERVAESVELMNLKHVVITAVARDDLRDAGS
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NVYAETVRKVRERNPFTTIEILPSDMGGDYDALETLMASRPDILNHNIETVRRLTPRVRA
HHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHH
RATYDRTLEFLRRSKELQPDIPTKSSIMVGLGETIEEIYETMDDLRANDVDILTIGQYLQ
HHHHHHHHHHHHHHHCCCCCCCCCCCEEECHHHHHHHHHHHHHHHCCCCCEEEECCHHHC
PSRKHLKVQKYYTPLEFGKLRKVAMDKGFKHCQAGPLVRSSYHADEQVNEAAKEKQRQGE
CHHHHEEHHHHCCCHHHHHHHHHHHHHCHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHCC
AQLNS
CCCCC
>Mature Secondary Structure 
ATKNEEILRKPDWLKIKLNTNENYTGLKKMMRKKNLNTVCEEAKCPNIHECWGARRTAT
CCCCHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHCCCCCHHHHHCCCHHHH
FMILGAVCTRACRFCAVKTGLPNELDLNEPERVAESVELMNLKHVVITAVARDDLRDAGS
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NVYAETVRKVRERNPFTTIEILPSDMGGDYDALETLMASRPDILNHNIETVRRLTPRVRA
HHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHH
RATYDRTLEFLRRSKELQPDIPTKSSIMVGLGETIEEIYETMDDLRANDVDILTIGQYLQ
HHHHHHHHHHHHHHHCCCCCCCCCCCEEECHHHHHHHHHHHHHHHCCCCCEEEECCHHHC
PSRKHLKVQKYYTPLEFGKLRKVAMDKGFKHCQAGPLVRSSYHADEQVNEAAKEKQRQGE
CHHHHEEHHHHCCCHHHHHHHHHHHHHCHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHCC
AQLNS
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA