Click here to switch to the map view.

The map label for this gene is gcvH

Identifier: 82750516

GI number: 82750516

Start: 834886

End: 835266

Strand: Direct

Name: gcvH

Synonym: SAB0764

Alternate gene names: 82750516

Gene position: 834886-835266 (Clockwise)

Preceding gene: 82750515

Following gene: 82750517

Centisome position: 30.44

GC content: 33.6

Gene sequence:

>381_bases
TTGGCAGTACCAAATGAATTGAAATATTCAAATGAGCATGAATGGGTTAAAGTTGAAGGTAATGTAGCAACAATTGGAAT
CACAGAATACGCACAAAGCGAGTTAGGTGATATTGTTTTTGTTGAATTACCAGAAACAGATGATGAAATTAATGAAGGGG
ATACGTTTGGTAGCGTAGAATCAGTTAAAACTGTATCAGAATTATATGCACCAATCTCTGGTAAAGTAGTTGAAGTCAAC
GAAGAACTAGAAGATAGTCCCGAATTTGTAAATGAATCTCCATACGAAAAAGCATGGATGGTAAAAGTAGAAATTAGTGA
TGAAAGTCAGCTTGAAGCTTTATTAACAGCTGAAAAATATTCAGAAATGATTGGTGAATAA

Upstream 100 bases:

>100_bases
AAATAAGCTATACAATTGTTAATTTTTATAAATATAGTTGAATAGCATCTAGCCTTATGGCATCATTAATGATGTAAAGA
TTAATTAGGAGGGGATTCTC

Downstream 100 bases:

>100_bases
TCACCGTGTAACTCCTTAATCTAAGATTGAGGAGTTTATTTTTAGTCTGAGTAAGTAAATAATATGAAAGAAAATTTATT
AGGTGCTATTATTTGGAGTA

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 126; Mature: 125

Protein sequence:

>126_residues
MAVPNELKYSNEHEWVKVEGNVATIGITEYAQSELGDIVFVELPETDDEINEGDTFGSVESVKTVSELYAPISGKVVEVN
EELEDSPEFVNESPYEKAWMVKVEISDESQLEALLTAEKYSEMIGE

Sequences:

>Translated_126_residues
MAVPNELKYSNEHEWVKVEGNVATIGITEYAQSELGDIVFVELPETDDEINEGDTFGSVESVKTVSELYAPISGKVVEVN
EELEDSPEFVNESPYEKAWMVKVEISDESQLEALLTAEKYSEMIGE
>Mature_125_residues
AVPNELKYSNEHEWVKVEGNVATIGITEYAQSELGDIVFVELPETDDEINEGDTFGSVESVKTVSELYAPISGKVVEVNE
ELEDSPEFVNESPYEKAWMVKVEISDESQLEALLTAEKYSEMIGE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=117, Percent_Identity=45.2991452991453, Blast_Score=123, Evalue=4e-29,
Organism=Homo sapiens, GI89057342, Length=117, Percent_Identity=44.4444444444444, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1789271, Length=123, Percent_Identity=52.0325203252033, Blast_Score=133, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17551294, Length=118, Percent_Identity=50, Blast_Score=131, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17507493, Length=115, Percent_Identity=47.8260869565217, Blast_Score=125, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6319272, Length=125, Percent_Identity=41.6, Blast_Score=109, Evalue=1e-25,
Organism=Drosophila melanogaster, GI17865652, Length=123, Percent_Identity=47.9674796747967, Blast_Score=130, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 14067; Mature: 13935

Theoretical pI: Translated: 3.63; Mature: 3.63

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVPNELKYSNEHEWVKVEGNVATIGITEYAQSELGDIVFVELPETDDEINEGDTFGSVE
CCCCCCCCCCCCCCEEEEECCEEEEEEHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHH
SVKTVSELYAPISGKVVEVNEELEDSPEFVNESPYEKAWMVKVEISDESQLEALLTAEKY
HHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHH
SEMIGE
HHHHCC
>Mature Secondary Structure 
AVPNELKYSNEHEWVKVEGNVATIGITEYAQSELGDIVFVELPETDDEINEGDTFGSVE
CCCCCCCCCCCCCEEEEECCEEEEEEHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHH
SVKTVSELYAPISGKVVEVNEELEDSPEFVNESPYEKAWMVKVEISDESQLEALLTAEKY
HHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHH
SEMIGE
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA