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The map label for this gene is pgk

Identifier: 82750481

GI number: 82750481

Start: 801747

End: 802937

Strand: Direct

Name: pgk

Synonym: SAB0729

Alternate gene names: 82750481

Gene position: 801747-802937 (Clockwise)

Preceding gene: 82750480

Following gene: 82750482

Centisome position: 29.23

GC content: 34.34

Gene sequence:

>1191_bases
ATGGCTAAAAAAATTGTTTCTGATTTAGATCTTAAAGGTAAAACAGTCATAGTACGTGCTGATTTTAACGTACCTTTAAA
AGACGGTGAAATTACTAATGACAACCGTATCGTTCAAGCTTTACCTACAATTCAATACATCATCGAACAAGGTGGTAAAA
TCGTACTATTTTCACATTTAGGTAAAGTAAAAGAAGAAAGTGATAAAGCAAAATTAACTTTACGTCCAGTTGCTGAAGAC
TTATCTAAGAAATTAGATAAAGAAGTTGTTTTCGTACCGGAAACACGCGGCGAAAAACTTGAAGCAGCTATTAAAGATCT
TAAAGAAGGCGACGTATTATTAGTTGAAAATACACGTTATGAAGATTTAGACGGTAAAAAAGAATCTAAAAATGATCCAG
AATTAGGTAAATACTGGGCATCTTTAGGTGATGTGTTTGTAAATGATGCTTTTGGTACTGCGCATCGTGAGCATGCATCT
AATGTTGGTATTTCTACACATTTAGAAACTGCAGCTGGATTCTTAATGGATAAAGAAATTAAGTTTATCGGTGGCGTAGT
TAACGATCCACATAAACCAGTTGTTGCTATTTTAGGTGGAGCAAAAGTATCTGACAAAATTAATGTCATCAAAAACTTAG
TTAACATTGCCGATAAAATTATCATCGGCGGAGGTATGGCTTATACTTTCTTAAAAGCTCAAGGTAAAGAAATTGGTATT
TCATTATTAGAAGAAGATAAAATCGACTTCGCAAAAGATTTATTAGAAAAACATGGTGATAAAATTGTATTACCAGTAGA
CACTAAAGTTGCTAAAGAATTTTCTAATGATGCCAAAATTACTGTAGTACCATCTGATTCAATTCCAGCAGACCAAGAAG
GTATGGATATTGGACCAAACACTGTAAAATTATTTGCAGATGAATTAGAAGGTGCGCACACTGTTGTATGGAATGGACCT
ATGGGTGTATTCGAGTTCAGTAACTTTGCACAAGGTACAATTGGTGTATGTAAAGCAATTGCAAACCTTAAAGATGCAAT
TACGATTATCGGTGGCGGTGATTCAGCTGCAGCAGCAATCTCTTTAGGTTTTGAAAATGATTTCACTCATATTTCAACTG
GTGGCGGAGCGTCATTAGAGTACCTAGAAGGTAAAGAATTGCCTGGTATCAAAGCAATCAATAATAAATAA

Upstream 100 bases:

>100_bases
AACTATTTAGCATTAATTAAAGCTTATGAGTAAGCGGGGAGCACAAACGCTTCTCCGCTTATTTTTATATAAAATTTCCT
AATTACAAGGAGGAAACACC

Downstream 100 bases:

>100_bases
TAAAGTGATAGTTTAAAGTGATGTGGCATGTTTGTTTAACATTGTTACGGGAAAACAGTCACAAGATGACATCGTGTTTC
ATCACTTTTTAAAAATATTT

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKKIVSDLDLKGKTVIVRADFNVPLKDGEITNDNRIVQALPTIQYIIEQGGKIVLFSHLGKVKEESDKAKLTLRPVAED
LSKKLDKEVVFVPETRGEKLEAAIKDLKEGDVLLVENTRYEDLDGKKESKNDPELGKYWASLGDVFVNDAFGTAHREHAS
NVGISTHLETAAGFLMDKEIKFIGGVVNDPHKPVVAILGGAKVSDKINVIKNLVNIADKIIIGGGMAYTFLKAQGKEIGI
SLLEEDKIDFAKDLLEKHGDKIVLPVDTKVAKEFSNDAKITVVPSDSIPADQEGMDIGPNTVKLFADELEGAHTVVWNGP
MGVFEFSNFAQGTIGVCKAIANLKDAITIIGGGDSAAAAISLGFENDFTHISTGGGASLEYLEGKELPGIKAINNK

Sequences:

>Translated_396_residues
MAKKIVSDLDLKGKTVIVRADFNVPLKDGEITNDNRIVQALPTIQYIIEQGGKIVLFSHLGKVKEESDKAKLTLRPVAED
LSKKLDKEVVFVPETRGEKLEAAIKDLKEGDVLLVENTRYEDLDGKKESKNDPELGKYWASLGDVFVNDAFGTAHREHAS
NVGISTHLETAAGFLMDKEIKFIGGVVNDPHKPVVAILGGAKVSDKINVIKNLVNIADKIIIGGGMAYTFLKAQGKEIGI
SLLEEDKIDFAKDLLEKHGDKIVLPVDTKVAKEFSNDAKITVVPSDSIPADQEGMDIGPNTVKLFADELEGAHTVVWNGP
MGVFEFSNFAQGTIGVCKAIANLKDAITIIGGGDSAAAAISLGFENDFTHISTGGGASLEYLEGKELPGIKAINNK
>Mature_395_residues
AKKIVSDLDLKGKTVIVRADFNVPLKDGEITNDNRIVQALPTIQYIIEQGGKIVLFSHLGKVKEESDKAKLTLRPVAEDL
SKKLDKEVVFVPETRGEKLEAAIKDLKEGDVLLVENTRYEDLDGKKESKNDPELGKYWASLGDVFVNDAFGTAHREHASN
VGISTHLETAAGFLMDKEIKFIGGVVNDPHKPVVAILGGAKVSDKINVIKNLVNIADKIIIGGGMAYTFLKAQGKEIGIS
LLEEDKIDFAKDLLEKHGDKIVLPVDTKVAKEFSNDAKITVVPSDSIPADQEGMDIGPNTVKLFADELEGAHTVVWNGPM
GVFEFSNFAQGTIGVCKAIANLKDAITIIGGGDSAAAAISLGFENDFTHISTGGGASLEYLEGKELPGIKAINNK

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=415, Percent_Identity=43.6144578313253, Blast_Score=320, Evalue=2e-87,
Organism=Homo sapiens, GI31543397, Length=415, Percent_Identity=42.8915662650602, Blast_Score=310, Evalue=1e-84,
Organism=Escherichia coli, GI1789294, Length=392, Percent_Identity=43.8775510204082, Blast_Score=303, Evalue=1e-83,
Organism=Caenorhabditis elegans, GI17508823, Length=413, Percent_Identity=46.9733656174334, Blast_Score=344, Evalue=4e-95,
Organism=Saccharomyces cerevisiae, GI6319857, Length=413, Percent_Identity=46.0048426150121, Blast_Score=325, Evalue=9e-90,
Organism=Drosophila melanogaster, GI24581203, Length=410, Percent_Identity=43.1707317073171, Blast_Score=304, Evalue=6e-83,
Organism=Drosophila melanogaster, GI17136394, Length=409, Percent_Identity=43.2762836185819, Blast_Score=301, Evalue=5e-82,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 42602; Mature: 42471

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKIVSDLDLKGKTVIVRADFNVPLKDGEITNDNRIVQALPTIQYIIEQGGKIVLFSHL
CCCHHHHHCCCCCCEEEEEECCCCCCCCCEECCCCHHHHHHHHHHHHHHCCCCEEEEECC
GKVKEESDKAKLTLRPVAEDLSKKLDKEVVFVPETRGEKLEAAIKDLKEGDVLLVENTRY
CCHHCCCCCCEEEEHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCEEEEECCCC
EDLDGKKESKNDPELGKYWASLGDVFVNDAFGTAHREHASNVGISTHLETAAGFLMDKEI
CCCCCCCCCCCCCHHHHHHHHHHHHHEECCCCCHHHHHHCCCCCEEHHHHHHHHHHCCCH
KFIGGVVNDPHKPVVAILGGAKVSDKINVIKNLVNIADKIIIGGGMAYTFLKAQGKEIGI
HEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHEEECCCCEEEEEECCCCEECE
SLLEEDKIDFAKDLLEKHGDKIVLPVDTKVAKEFSNDAKITVVPSDSIPADQEGMDIGPN
EEECCCCHHHHHHHHHHCCCEEEEECCHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCC
TVKLFADELEGAHTVVWNGPMGVFEFSNFAQGTIGVCKAIANLKDAITIIGGGDSAAAAI
HHEEEHHHCCCCEEEEECCCCCEEECCCCCCCHHHHHHHHHCCCEEEEEEECCCCCEEEE
SLGFENDFTHISTGGGASLEYLEGKELPGIKAINNK
EECCCCCCCEEECCCCCEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
AKKIVSDLDLKGKTVIVRADFNVPLKDGEITNDNRIVQALPTIQYIIEQGGKIVLFSHL
CCHHHHHCCCCCCEEEEEECCCCCCCCCEECCCCHHHHHHHHHHHHHHCCCCEEEEECC
GKVKEESDKAKLTLRPVAEDLSKKLDKEVVFVPETRGEKLEAAIKDLKEGDVLLVENTRY
CCHHCCCCCCEEEEHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCEEEEECCCC
EDLDGKKESKNDPELGKYWASLGDVFVNDAFGTAHREHASNVGISTHLETAAGFLMDKEI
CCCCCCCCCCCCCHHHHHHHHHHHHHEECCCCCHHHHHHCCCCCEEHHHHHHHHHHCCCH
KFIGGVVNDPHKPVVAILGGAKVSDKINVIKNLVNIADKIIIGGGMAYTFLKAQGKEIGI
HEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHEEECCCCEEEEEECCCCEECE
SLLEEDKIDFAKDLLEKHGDKIVLPVDTKVAKEFSNDAKITVVPSDSIPADQEGMDIGPN
EEECCCCHHHHHHHHHHCCCEEEEECCHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCC
TVKLFADELEGAHTVVWNGPMGVFEFSNFAQGTIGVCKAIANLKDAITIIGGGDSAAAAI
HHEEEHHHCCCCEEEEECCCCCEEECCCCCCCHHHHHHHHHCCCEEEEEEECCCCCEEEE
SLGFENDFTHISTGGGASLEYLEGKELPGIKAINNK
EECCCCCCCEEECCCCCEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA