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The map label for this gene is gltD

Identifier: 82750180

GI number: 82750180

Start: 462177

End: 463640

Strand: Direct

Name: gltD

Synonym: SAB0422

Alternate gene names: 82750180

Gene position: 462177-463640 (Clockwise)

Preceding gene: 82750179

Following gene: 82750181

Centisome position: 16.85

GC content: 38.32

Gene sequence:

>1464_bases
ATGGGCGAATTTAAAGGATTTATGAAGTATGACAAACAGTACTTAGGTGAATTATCACTGGTAGACCGTTTGAAGCATCA
TAAAGCATATCAACAACGATTTACTAAAGAAGATGCCTCTATCCAAGGTGCGCGATGTATGGATTGTGGAACGCCGTTTT
GTCAAACCGGACAACAGTATGGTAGGGAAACAATAGGTTGTCCAATTGGAAACTACATTCCTGAATGGAACGACTTAGTG
TATCATCAAGATTTTAAAACTGCTTATGAACGCTTAAGTGAAACAAATAACTTTCCTGACTTTACAGGGCGTGTATGTCC
TGCACCATGTGAAAGTGCTTGTGTTATGAAGATTAATAGAGAATCGATTGCGATTAAAGGTATTGAACGCACAATTATTG
ATGAAGCTTTTGAAAATGGTTGGGTAGCGCCGAAAGTTCCGAGTCGCCGTAGAGATGAAAAAGTAGCAATCGTTGGAAGC
GGTCCAGCAGGATTAACTGCTGCTGAAGAACTTAATCTACTAGGGTATCAAGTGACTATTTATGAACGTGCTAGAGAATC
AGGCGGTTTATTAATGTATGGTATTCCGAATATGAAACTTGATAAAGATGTGGTTCGACGTCGTATTAAGTTAATGGAAG
AAGCGGGCATTACTTTCATTAATGGTGTTGAAGTAGGCGTTGATATTGATAAAGCAACGTTAGAATCTGAGTATGATGCC
ATTATATTATGTACTGGTGCACAAAAAGGTAGAGATTTACCTTTAGAAGGACGCATGGGTGATGGTATACATTTCGCTAT
GGATTATTTAACTGAACAAACGCAGCTGTTAAATGGAGAAATTGATGATATAACAATAACTGCAAAAGATAAGAATGTCA
TTATCATTGGTGCTGGTGATACAGGGGCAGACTGTGTAGCGACAGCATTAAGAGAAAATTGTAAATCGATTGTTCAATTT
AATAAATATACGAAATTGCCAGAAGCAATTACATTTACAGAAAATGCATCATGGCCTTTAGCAATGCCGGTGTTTAAAAT
GGACTATGCGCACCAAGAGTACGAAGCTAAGTTTGGTAAGGAACCACGTGCATATGGTGTTCAAACGATGCGTTACGATG
TTGACGATAAAGGACACATACGTGGTTTGTATACTCAAATTTTAGAGCAAGGCGAAAATGGTATGGTCATGAAAGAAGGA
CCTGAAAGATTTTGGCCTGCTGACCTTGTATTATTATCAATCGGCTTCGAAGGTACAGAACCAACAGTACCGAATTCTTT
TAACATTAAAACGGATAGAAATCGAATCGTGGCGGATGATACAAACTATCAAACTAATAATGAAAAGGTATTTGCTGCTG
GAGATGCTAGACGTGGTCAAAGTTTAGTTGTATGGGCGATTAAAGAAGGTAGAGGCGTAGCGAAAGCAGTAGATCAGTAT
TTAGCGAGTAAAGTTTGTGTATAA

Upstream 100 bases:

>100_bases
ATGAAGATGAAGCGATGTTAGCTGCATTTTACGATGACAGTAAAACAATCGACGCTAAACATAAACCAGCCGTTGTGTAT
TAAGGAAAGGGGGAGATACG

Downstream 100 bases:

>100_bases
TCTTTGTATGGAAATGGTGGTTACGTTGACGTTGTGTCATGCTGAATCGAGTTTGAAATAATCTAGTATCTATCAATGTC
ACATGTGCCATCTTTGTAAC

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MGEFKGFMKYDKQYLGELSLVDRLKHHKAYQQRFTKEDASIQGARCMDCGTPFCQTGQQYGRETIGCPIGNYIPEWNDLV
YHQDFKTAYERLSETNNFPDFTGRVCPAPCESACVMKINRESIAIKGIERTIIDEAFENGWVAPKVPSRRRDEKVAIVGS
GPAGLTAAEELNLLGYQVTIYERARESGGLLMYGIPNMKLDKDVVRRRIKLMEEAGITFINGVEVGVDIDKATLESEYDA
IILCTGAQKGRDLPLEGRMGDGIHFAMDYLTEQTQLLNGEIDDITITAKDKNVIIIGAGDTGADCVATALRENCKSIVQF
NKYTKLPEAITFTENASWPLAMPVFKMDYAHQEYEAKFGKEPRAYGVQTMRYDVDDKGHIRGLYTQILEQGENGMVMKEG
PERFWPADLVLLSIGFEGTEPTVPNSFNIKTDRNRIVADDTNYQTNNEKVFAAGDARRGQSLVVWAIKEGRGVAKAVDQY
LASKVCV

Sequences:

>Translated_487_residues
MGEFKGFMKYDKQYLGELSLVDRLKHHKAYQQRFTKEDASIQGARCMDCGTPFCQTGQQYGRETIGCPIGNYIPEWNDLV
YHQDFKTAYERLSETNNFPDFTGRVCPAPCESACVMKINRESIAIKGIERTIIDEAFENGWVAPKVPSRRRDEKVAIVGS
GPAGLTAAEELNLLGYQVTIYERARESGGLLMYGIPNMKLDKDVVRRRIKLMEEAGITFINGVEVGVDIDKATLESEYDA
IILCTGAQKGRDLPLEGRMGDGIHFAMDYLTEQTQLLNGEIDDITITAKDKNVIIIGAGDTGADCVATALRENCKSIVQF
NKYTKLPEAITFTENASWPLAMPVFKMDYAHQEYEAKFGKEPRAYGVQTMRYDVDDKGHIRGLYTQILEQGENGMVMKEG
PERFWPADLVLLSIGFEGTEPTVPNSFNIKTDRNRIVADDTNYQTNNEKVFAAGDARRGQSLVVWAIKEGRGVAKAVDQY
LASKVCV
>Mature_486_residues
GEFKGFMKYDKQYLGELSLVDRLKHHKAYQQRFTKEDASIQGARCMDCGTPFCQTGQQYGRETIGCPIGNYIPEWNDLVY
HQDFKTAYERLSETNNFPDFTGRVCPAPCESACVMKINRESIAIKGIERTIIDEAFENGWVAPKVPSRRRDEKVAIVGSG
PAGLTAAEELNLLGYQVTIYERARESGGLLMYGIPNMKLDKDVVRRRIKLMEEAGITFINGVEVGVDIDKATLESEYDAI
ILCTGAQKGRDLPLEGRMGDGIHFAMDYLTEQTQLLNGEIDDITITAKDKNVIIIGAGDTGADCVATALRENCKSIVQFN
KYTKLPEAITFTENASWPLAMPVFKMDYAHQEYEAKFGKEPRAYGVQTMRYDVDDKGHIRGLYTQILEQGENGMVMKEGP
ERFWPADLVLLSIGFEGTEPTVPNSFNIKTDRNRIVADDTNYQTNNEKVFAAGDARRGQSLVVWAIKEGRGVAKAVDQYL
ASKVCV

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=469, Percent_Identity=24.3070362473348, Blast_Score=90, Evalue=4e-18,
Organism=Escherichia coli, GI1788811, Length=468, Percent_Identity=33.5470085470085, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1789606, Length=463, Percent_Identity=33.2613390928726, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI87082180, Length=430, Percent_Identity=33.7209302325581, Blast_Score=191, Evalue=9e-50,
Organism=Escherichia coli, GI1788468, Length=454, Percent_Identity=27.9735682819383, Blast_Score=131, Evalue=8e-32,
Organism=Escherichia coli, GI1789243, Length=464, Percent_Identity=23.9224137931034, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17570289, Length=501, Percent_Identity=41.9161676646707, Blast_Score=383, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71984108, Length=482, Percent_Identity=23.8589211618257, Blast_Score=82, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6320030, Length=485, Percent_Identity=41.4432989690722, Blast_Score=392, Evalue=1e-110,
Organism=Drosophila melanogaster, GI28574881, Length=487, Percent_Identity=47.2279260780287, Blast_Score=447, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24665539, Length=487, Percent_Identity=47.2279260780287, Blast_Score=447, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24665547, Length=487, Percent_Identity=47.2279260780287, Blast_Score=446, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24665543, Length=487, Percent_Identity=47.2279260780287, Blast_Score=446, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24640763, Length=313, Percent_Identity=27.4760383386581, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI18858217, Length=313, Percent_Identity=27.4760383386581, Blast_Score=88, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 54475; Mature: 54343

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEFKGFMKYDKQYLGELSLVDRLKHHKAYQQRFTKEDASIQGARCMDCGTPFCQTGQQY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHCHHHH
GRETIGCPIGNYIPEWNDLVYHQDFKTAYERLSETNNFPDFTGRVCPAPCESACVMKINR
CCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECC
ESIAIKGIERTIIDEAFENGWVAPKVPSRRRDEKVAIVGSGPAGLTAAEELNLLGYQVTI
CEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHEEEEEEEE
YERARESGGLLMYGIPNMKLDKDVVRRRIKLMEEAGITFINGVEVGVDIDKATLESEYDA
EEHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEECEEEEEECCHHHHCCCCCE
IILCTGAQKGRDLPLEGRMGDGIHFAMDYLTEQTQLLNGEIDDITITAKDKNVIIIGAGD
EEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEECCC
TGADCVATALRENCKSIVQFNKYTKLPEAITFTENASWPLAMPVFKMDYAHQEYEAKFGK
CCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEECCHHHHHHHHHHHHHHCCC
EPRAYGVQTMRYDVDDKGHIRGLYTQILEQGENGMVMKEGPERFWPADLVLLSIGFEGTE
CCHHCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEEEEECCCCCC
PTVPNSFNIKTDRNRIVADDTNYQTNNEKVFAAGDARRGQSLVVWAIKEGRGVAKAVDQY
CCCCCCEEEECCCCEEEECCCCCCCCCCEEEEECCCCCCCEEEEEEEECCCCHHHHHHHH
LASKVCV
HHHHCCC
>Mature Secondary Structure 
GEFKGFMKYDKQYLGELSLVDRLKHHKAYQQRFTKEDASIQGARCMDCGTPFCQTGQQY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHCHHHH
GRETIGCPIGNYIPEWNDLVYHQDFKTAYERLSETNNFPDFTGRVCPAPCESACVMKINR
CCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECC
ESIAIKGIERTIIDEAFENGWVAPKVPSRRRDEKVAIVGSGPAGLTAAEELNLLGYQVTI
CEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHEEEEEEEE
YERARESGGLLMYGIPNMKLDKDVVRRRIKLMEEAGITFINGVEVGVDIDKATLESEYDA
EEHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEECEEEEEECCHHHHCCCCCE
IILCTGAQKGRDLPLEGRMGDGIHFAMDYLTEQTQLLNGEIDDITITAKDKNVIIIGAGD
EEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEECCC
TGADCVATALRENCKSIVQFNKYTKLPEAITFTENASWPLAMPVFKMDYAHQEYEAKFGK
CCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEECCHHHHHHHHHHHHHHCCC
EPRAYGVQTMRYDVDDKGHIRGLYTQILEQGENGMVMKEGPERFWPADLVLLSIGFEGTE
CCHHCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEEEEECCCCCC
PTVPNSFNIKTDRNRIVADDTNYQTNNEKVFAAGDARRGQSLVVWAIKEGRGVAKAVDQY
CCCCCCEEEECCCCEEEECCCCCCCCCCEEEEECCCCCCCEEEEEEEECCCCHHHHHHHH
LASKVCV
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]