Click here to switch to the map view.

The map label for this gene is mccB [H]

Identifier: 82750168

GI number: 82750168

Start: 447594

End: 448736

Strand: Direct

Name: mccB [H]

Synonym: SAB0410

Alternate gene names: 82750168

Gene position: 447594-448736 (Clockwise)

Preceding gene: 82750167

Following gene: 82750169

Centisome position: 16.32

GC content: 35.7

Gene sequence:

>1143_bases
ATGAATAAGAAAACTAAATTAATTCACGGTGGACACACAACGGACGACTATACAGGTGCAGTTACAACACCAATTTATCA
AACAAGTACATATTTACAAGATGATATTGGTGATTTACGTCAAGGATATGAATATTCTCGTACTGCGAATCCAACAAGAA
GTTCTGTAGAAAGCGTTATTGCGGCATTAGAAAATGGCAAACATGGCTTTGCATTTAGTTCAGGTGTTGCAGCAATCAGT
GCAGTTGTTATGCTGTTGGACAAAGGTGATCATATTATTTTAAATTCAGATGTATACGGCGGTACTTATCGCGCATTGAC
AAAAGTATTTACACGATTTGGCATTGAAGTGGATTTTGTAGATACAACGCATACAGATTCAATTGTACAAGCGATACGCC
CAACAACAAAAATGTTGTTTATTGAAACACCTTCTAATCCATTATTACGTGTTACTGACATTAAAAAGTCTGCTGAAATT
GCGAAAGAACACGGTTTGATTTCAGTCGTTGATAACACATTTATGACACCTTATTATCAGAATCCATTAGATTTAGGCAT
CGATATTGTCTTACATTCTGCAACGAAATATTTAGGTGGACATAGTGATGTCGTTGCTGGTTTAGTTGCAACATCGGATG
ACAAGCTTGCAGAACGTTTAGCATTTATTTCAAATTCAACAGGTGGCATTTTAGGACCTCAAGATAGCTATTTACTTGTG
AGGGGTATTAAAACATTAGGTTTACGTATGGAACAAATTAATCGCAGTGTTATTGAAATTATTAAAATGTTACAAGCACA
TCCAGCTGTGCAACAAGTGTTCCATCCAAGTATTGAAAGTCATTTAAATCATGATGTCCATATGGCTCAAGCGGATGGCC
ATACAGGTGTGATTGCATTTGAAGTAAAAGATACAGAAAGTGCCAAACAATTGATTAAAGCAACATCGTATTACACATTA
GCTGAAAGTTTAGGTGCAGTAGAAAGTTTAATTTCAGTACCTGCACTGATGACACATGCATCCATTCCAGCAGATATTCG
AGCTAAAGAAGGTATTACAGACGGACTTGTAAGAATTTCTGTAGGTATTGAAGATACTGAAGATTTAGTAGATGATTTAA
AACAAGCACTAGATACGTTATAA

Upstream 100 bases:

>100_bases
TTAAAAGCGCAATTATCTGAAGGTACGATTGTTGTCGTATTTCCAGATGGTAGCGATCGATATATGTCTAAGGAAATATT
TAATTTTGAGGAGAATGATT

Downstream 100 bases:

>100_bases
ATAATAGCAGCACTGGCATATATTTTGAGTTCAACGTTTGTTGTTCTTGAATATGCTAGTGCTTTTTTAGTGTGATGCTA
ATATTTCGTAATTGCGTTGT

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: Gamma-cystathionase; Homocysteine gamma-lyase [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MNKKTKLIHGGHTTDDYTGAVTTPIYQTSTYLQDDIGDLRQGYEYSRTANPTRSSVESVIAALENGKHGFAFSSGVAAIS
AVVMLLDKGDHIILNSDVYGGTYRALTKVFTRFGIEVDFVDTTHTDSIVQAIRPTTKMLFIETPSNPLLRVTDIKKSAEI
AKEHGLISVVDNTFMTPYYQNPLDLGIDIVLHSATKYLGGHSDVVAGLVATSDDKLAERLAFISNSTGGILGPQDSYLLV
RGIKTLGLRMEQINRSVIEIIKMLQAHPAVQQVFHPSIESHLNHDVHMAQADGHTGVIAFEVKDTESAKQLIKATSYYTL
AESLGAVESLISVPALMTHASIPADIRAKEGITDGLVRISVGIEDTEDLVDDLKQALDTL

Sequences:

>Translated_380_residues
MNKKTKLIHGGHTTDDYTGAVTTPIYQTSTYLQDDIGDLRQGYEYSRTANPTRSSVESVIAALENGKHGFAFSSGVAAIS
AVVMLLDKGDHIILNSDVYGGTYRALTKVFTRFGIEVDFVDTTHTDSIVQAIRPTTKMLFIETPSNPLLRVTDIKKSAEI
AKEHGLISVVDNTFMTPYYQNPLDLGIDIVLHSATKYLGGHSDVVAGLVATSDDKLAERLAFISNSTGGILGPQDSYLLV
RGIKTLGLRMEQINRSVIEIIKMLQAHPAVQQVFHPSIESHLNHDVHMAQADGHTGVIAFEVKDTESAKQLIKATSYYTL
AESLGAVESLISVPALMTHASIPADIRAKEGITDGLVRISVGIEDTEDLVDDLKQALDTL
>Mature_380_residues
MNKKTKLIHGGHTTDDYTGAVTTPIYQTSTYLQDDIGDLRQGYEYSRTANPTRSSVESVIAALENGKHGFAFSSGVAAIS
AVVMLLDKGDHIILNSDVYGGTYRALTKVFTRFGIEVDFVDTTHTDSIVQAIRPTTKMLFIETPSNPLLRVTDIKKSAEI
AKEHGLISVVDNTFMTPYYQNPLDLGIDIVLHSATKYLGGHSDVVAGLVATSDDKLAERLAFISNSTGGILGPQDSYLLV
RGIKTLGLRMEQINRSVIEIIKMLQAHPAVQQVFHPSIESHLNHDVHMAQADGHTGVIAFEVKDTESAKQLIKATSYYTL
AESLGAVESLISVPALMTHASIPADIRAKEGITDGLVRISVGIEDTEDLVDDLKQALDTL

Specific function: Catalyzes the conversion of cystathionine to cysteine, and homocysteine to sulfide [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=376, Percent_Identity=45.7446808510638, Blast_Score=317, Evalue=1e-86,
Organism=Homo sapiens, GI299473759, Length=376, Percent_Identity=41.2234042553192, Blast_Score=268, Evalue=5e-72,
Organism=Homo sapiens, GI25453487, Length=375, Percent_Identity=39.2, Blast_Score=243, Evalue=2e-64,
Organism=Escherichia coli, GI1790375, Length=377, Percent_Identity=42.4403183023873, Blast_Score=297, Evalue=8e-82,
Organism=Escherichia coli, GI1789383, Length=387, Percent_Identity=27.1317829457364, Blast_Score=160, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17560056, Length=378, Percent_Identity=45.7671957671958, Blast_Score=333, Evalue=7e-92,
Organism=Caenorhabditis elegans, GI17538055, Length=378, Percent_Identity=46.031746031746, Blast_Score=325, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI71980708, Length=372, Percent_Identity=38.4408602150538, Blast_Score=257, Evalue=7e-69,
Organism=Saccharomyces cerevisiae, GI6319307, Length=382, Percent_Identity=46.8586387434555, Blast_Score=327, Evalue=2e-90,
Organism=Saccharomyces cerevisiae, GI6321254, Length=407, Percent_Identity=33.9066339066339, Blast_Score=202, Evalue=6e-53,
Organism=Saccharomyces cerevisiae, GI6323333, Length=429, Percent_Identity=30.7692307692308, Blast_Score=172, Evalue=6e-44,
Organism=Saccharomyces cerevisiae, GI6321170, Length=297, Percent_Identity=30.6397306397306, Blast_Score=170, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6321904, Length=394, Percent_Identity=28.9340101522843, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6322590, Length=278, Percent_Identity=26.6187050359712, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6323557, Length=347, Percent_Identity=26.2247838616715, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6322970, Length=304, Percent_Identity=23.6842105263158, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19922554, Length=380, Percent_Identity=47.3684210526316, Blast_Score=365, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.1; =4.4.1.2 [H]

Molecular weight: Translated: 41250; Mature: 41250

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKTKLIHGGHTTDDYTGAVTTPIYQTSTYLQDDIGDLRQGYEYSRTANPTRSSVESVI
CCCCCEEEECCCCCCCCCCCCCCCEECCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
AALENGKHGFAFSSGVAAISAVVMLLDKGDHIILNSDVYGGTYRALTKVFTRFGIEVDFV
HHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCEEEEE
DTTHTDSIVQAIRPTTKMLFIETPSNPLLRVTDIKKSAEIAKEHGLISVVDNTFMTPYYQ
CCCCHHHHHHHHCCCEEEEEEECCCCCCEEEECCHHHHHHHHHCCEEEEECCCCCCCCCC
NPLDLGIDIVLHSATKYLGGHSDVVAGLVATSDDKLAERLAFISNSTGGILGPQDSYLLV
CCHHCCHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCEEHH
RGIKTLGLRMEQINRSVIEIIKMLQAHPAVQQVFHPSIESHLNHDVHMAQADGHTGVIAF
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCHHHHCCCCEEEEECCCCCEEEEE
EVKDTESAKQLIKATSYYTLAESLGAVESLISVPALMTHASIPADIRAKEGITDGLVRIS
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCEEEEE
VGIEDTEDLVDDLKQALDTL
ECCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKKTKLIHGGHTTDDYTGAVTTPIYQTSTYLQDDIGDLRQGYEYSRTANPTRSSVESVI
CCCCCEEEECCCCCCCCCCCCCCCEECCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
AALENGKHGFAFSSGVAAISAVVMLLDKGDHIILNSDVYGGTYRALTKVFTRFGIEVDFV
HHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCEEEEE
DTTHTDSIVQAIRPTTKMLFIETPSNPLLRVTDIKKSAEIAKEHGLISVVDNTFMTPYYQ
CCCCHHHHHHHHCCCEEEEEEECCCCCCEEEECCHHHHHHHHHCCEEEEECCCCCCCCCC
NPLDLGIDIVLHSATKYLGGHSDVVAGLVATSDDKLAERLAFISNSTGGILGPQDSYLLV
CCHHCCHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCEEHH
RGIKTLGLRMEQINRSVIEIIKMLQAHPAVQQVFHPSIESHLNHDVHMAQADGHTGVIAF
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCHHHHCCCCEEEEECCCCCEEEEE
EVKDTESAKQLIKATSYYTLAESLGAVESLISVPALMTHASIPADIRAKEGITDGLVRIS
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCEEEEE
VGIEDTEDLVDDLKQALDTL
ECCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9308178; 9384377 [H]