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The map label for this gene is lplA [H]

Identifier: 82750039

GI number: 82750039

Start: 328813

End: 329835

Strand: Direct

Name: lplA [H]

Synonym: SAB0277

Alternate gene names: 82750039

Gene position: 328813-329835 (Clockwise)

Preceding gene: 82750038

Following gene: 82750045

Centisome position: 11.99

GC content: 34.02

Gene sequence:

>1023_bases
ATGTACTTAATAGAACCGATTAGAAATGGAGAATATATTACTGATGGTGCGATTGCACTCGCTATGCAAGTTTATGTTAA
CCAGAATATCTTTTTAGATGAAGATATTTTATTTCCTTATTATTGTGATCCAAAAGTGGAAATTGGACGTTTTCAAAATA
CTGCTATAGAAGTGAATCAAGATTATATAGATAAACACAGTATTCAAGTAGTTCGCCGAGATACTGGTGGTGGCGCTGTG
TATGTTGATAAAGGTGCCGTTAATATGTGTTGTATTTTACAACAAGACACTTCAATTTATGGTGATTTTCAACGATTTTA
TCAACCAGCTATAAAGGCGTTGCATACATTAGGTGCAACAGATGTGGTACAAAGCGGTAGAAATGATTTAACCTTGAATG
GTAAAAAAGTGTCAGGCGCCGCAATGACATTAATGAACAATCGTATTTATGGCGGTTATTCGCTATTACTTGATGTTAAT
TATGAAGCAATGGATAAAGTGTTAAAGCCTAATCGCAAAAAGATTGCATCGAAAGGGATTAAATCTGTGCGTGCACGTGT
TGGTCATCTTAGAGAAGCACTGGATGAAAAGTATCGGGATATAACCATTGAAGAATTTAAAAATTTAATGGTGACGCAGA
TTTTGGGAATCGATGACATTAAAGAGGCGAAACGATACGAATTATCTGATGCAGATTGGGAATCGATTGAAGAATTGGCG
AGTAAAAAGTATAAAAATTGGGATTGGAACTATGGGAAGTCACCTAAATATGAATATAATCGAAGTGAAAGATTATCATC
AGGTACTGTAGATATTACCATTTCTGTTGAACAAAATCGTATTGCAGATTGTAGAATTTTTGGTGATTTCTTTGGACAAG
GTGATATAAAAGATGTGGAACAAGCATTACAAGGAACAAAAATGACAAGAGAAGATTTAACGCATCAGTTAAAGCAATTA
GACATCGTTTATTATTTTGGTAATGTTACGGTTGAATCATTGGTTGAGATGATTTTAAGTTAA

Upstream 100 bases:

>100_bases
GAACGTACCATACATTTGACTGATGATATCTCAACATTGATTACAGCAGCACTCCGGAACGACTGCACAACGAAAAATAA
CAACATTGGAGAGACAGAAG

Downstream 100 bases:

>100_bases
TTTTGTTTAGGCGTTAATGTCGCACCCTCGTTAAACATTATTTAATATAATACAAAAACACCGTCCATAAGTAAGTGGCT
TTCCTCTTGATAAAAGGGGC

Product: lipoate-protein ligase

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MYLIEPIRNGEYITDGAIALAMQVYVNQNIFLDEDILFPYYCDPKVEIGRFQNTAIEVNQDYIDKHSIQVVRRDTGGGAV
YVDKGAVNMCCILQQDTSIYGDFQRFYQPAIKALHTLGATDVVQSGRNDLTLNGKKVSGAAMTLMNNRIYGGYSLLLDVN
YEAMDKVLKPNRKKIASKGIKSVRARVGHLREALDEKYRDITIEEFKNLMVTQILGIDDIKEAKRYELSDADWESIEELA
SKKYKNWDWNYGKSPKYEYNRSERLSSGTVDITISVEQNRIADCRIFGDFFGQGDIKDVEQALQGTKMTREDLTHQLKQL
DIVYYFGNVTVESLVEMILS

Sequences:

>Translated_340_residues
MYLIEPIRNGEYITDGAIALAMQVYVNQNIFLDEDILFPYYCDPKVEIGRFQNTAIEVNQDYIDKHSIQVVRRDTGGGAV
YVDKGAVNMCCILQQDTSIYGDFQRFYQPAIKALHTLGATDVVQSGRNDLTLNGKKVSGAAMTLMNNRIYGGYSLLLDVN
YEAMDKVLKPNRKKIASKGIKSVRARVGHLREALDEKYRDITIEEFKNLMVTQILGIDDIKEAKRYELSDADWESIEELA
SKKYKNWDWNYGKSPKYEYNRSERLSSGTVDITISVEQNRIADCRIFGDFFGQGDIKDVEQALQGTKMTREDLTHQLKQL
DIVYYFGNVTVESLVEMILS
>Mature_340_residues
MYLIEPIRNGEYITDGAIALAMQVYVNQNIFLDEDILFPYYCDPKVEIGRFQNTAIEVNQDYIDKHSIQVVRRDTGGGAV
YVDKGAVNMCCILQQDTSIYGDFQRFYQPAIKALHTLGATDVVQSGRNDLTLNGKKVSGAAMTLMNNRIYGGYSLLLDVN
YEAMDKVLKPNRKKIASKGIKSVRARVGHLREALDEKYRDITIEEFKNLMVTQILGIDDIKEAKRYELSDADWESIEELA
SKKYKNWDWNYGKSPKYEYNRSERLSSGTVDITISVEQNRIADCRIFGDFFGQGDIKDVEQALQGTKMTREDLTHQLKQL
DIVYYFGNVTVESLVEMILS

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Homo sapiens, GI7706252, Length=306, Percent_Identity=27.1241830065359, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI21729884, Length=306, Percent_Identity=27.1241830065359, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI21729882, Length=306, Percent_Identity=27.1241830065359, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI21729880, Length=306, Percent_Identity=27.1241830065359, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI21729878, Length=306, Percent_Identity=27.1241830065359, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1790846, Length=314, Percent_Identity=27.3885350318471, Blast_Score=113, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71981909, Length=257, Percent_Identity=31.5175097276265, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI28573464, Length=327, Percent_Identity=26.9113149847095, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 38825; Mature: 38825

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLIEPIRNGEYITDGAIALAMQVYVNQNIFLDEDILFPYYCDPKVEIGRFQNTAIEVNQ
CEEECCCCCCCEEECHHHHHHHHHHHCCCEEECCCCCCEEECCCCCEECCCCCCEEEECH
DYIDKHSIQVVRRDTGGGAVYVDKGAVNMCCILQQDTSIYGDFQRFYQPAIKALHTLGAT
HHHCCCCEEEEEEECCCCEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCHH
DVVQSGRNDLTLNGKKVSGAAMTLMNNRIYGGYSLLLDVNYEAMDKVLKPNRKKIASKGI
HHHHCCCCCEEECCCEECCHHHHHHCCCEECCEEEEEECCHHHHHHHHCCCHHHHHHHHH
KSVRARVGHLREALDEKYRDITIEEFKNLMVTQILGIDDIKEAKRYELSDADWESIEELA
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHH
SKKYKNWDWNYGKSPKYEYNRSERLSSGTVDITISVEQNRIADCRIFGDFFGQGDIKDVE
HHCCCCCCCCCCCCCCCCCCHHHHCCCCCEEEEEEECCCCCCEEEEEEHHCCCCCHHHHH
QALQGTKMTREDLTHQLKQLDIVYYFGNVTVESLVEMILS
HHHHCCCCCHHHHHHHHHHEEEEEEECCCCHHHHHHHHCC
>Mature Secondary Structure
MYLIEPIRNGEYITDGAIALAMQVYVNQNIFLDEDILFPYYCDPKVEIGRFQNTAIEVNQ
CEEECCCCCCCEEECHHHHHHHHHHHCCCEEECCCCCCEEECCCCCEECCCCCCEEEECH
DYIDKHSIQVVRRDTGGGAVYVDKGAVNMCCILQQDTSIYGDFQRFYQPAIKALHTLGAT
HHHCCCCEEEEEEECCCCEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCHH
DVVQSGRNDLTLNGKKVSGAAMTLMNNRIYGGYSLLLDVNYEAMDKVLKPNRKKIASKGI
HHHHCCCCCEEECCCEECCHHHHHHCCCEECCEEEEEECCHHHHHHHHCCCHHHHHHHHH
KSVRARVGHLREALDEKYRDITIEEFKNLMVTQILGIDDIKEAKRYELSDADWESIEELA
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHH
SKKYKNWDWNYGKSPKYEYNRSERLSSGTVDITISVEQNRIADCRIFGDFFGQGDIKDVE
HHCCCCCCCCCCCCCCCCCCHHHHCCCCCEEEEEEECCCCCCEEEEEEHHCCCCCHHHHH
QALQGTKMTREDLTHQLKQLDIVYYFGNVTVESLVEMILS
HHHHCCCCCHHHHHHHHHHEEEEEEECCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]