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The map label for this gene is rfbX [H]

Identifier: 82749845

GI number: 82749845

Start: 89948

End: 91378

Strand: Direct

Name: rfbX [H]

Synonym: SAB0071

Alternate gene names: 82749845

Gene position: 89948-91378 (Clockwise)

Preceding gene: 82749844

Following gene: 82749846

Centisome position: 3.28

GC content: 31.73

Gene sequence:

>1431_bases
ATGAAGAGTGATTCACTAAAAGAAAATATTATTTATCAAGGGCTATACCAATTGATTAGAACGATGACACCACTGATTAC
AATACCCATTATTTCACGTGCATTTGGTCCCAGTGGTGTGGGTATTGTTTCATTTTCTTTCAATATCGTGCAATACTTTT
TGATGATTGCAAGTGTTGGTGTGCAGTTATATTTTAATAGAGTTATCGCCAAGTCCGTTAACGACAAACGGCAATTGTCT
CAGCAGTTTTGGGATATCTTTGTTAGTAAATTATTATTGTCGCTAACAGTTTTTGCGATTTATATCGCTGTGATTACAAT
CTTTATAGATGATTACTATCTTATTTTCCTACTACAAGGTATCTATATTATAGGTGCAGCACTCGATATTTCATGGTTTT
ATGCTGGAACTGAAAAGTTTAAAATACCTAGCCTTAGTAATATTGTTGCGTCTGGTATTGTATTAAGTGTAGTTGTTATC
TTTGTCAAAGATCAATCAGATTTATCATTGTATGTATTTACTATTGCTATTGTGACGGTATTAAACCAATTACCTTTGTT
TATCTATTTAAAACGATACATTAGCTTTGTTTCGGTTAATTGGATACACGTCTGGCAATTGTTTCGTTCGTCATTAGCAT
ACTTATTACCAAATGGACAGCTCAACTTATATACTAGTATTTCTTGCGTTGTTCTTGGTTTAGTAGGCACATACCAACAA
GTTGGTACCTTTTCTAACGCATTTAATATTTTAACGGTCGCAATCATAATGATTAATACATTTGATCTTGTAATGATTCC
GCGTATTACCAAAATGTCTATCCAGAAATCACATAGTTTAACTAAAACGTTAGCTAATAATATGAATATTCAATTGATAT
TAACAGTACCTATGGTCTTTGGTTTAATTGCAATTATGCCATCATTTTATTTATGGTTCTTTGGTGAGGAATTCGCATCA
ACTGTCCCATTGATGACCATTTTAGCGATACTTGTATTAATCATTCCTTTAAATATGTTGATAAGCAGGCAATATTTATT
AATAGTGAATAAAATAAGATTATATAATGCGTCAATTACTATTGGTGCGGTAATGAATTTAGTATTATGTCTTGTTTTGA
TATATTTTTATGGAATTTACGGTGCTGCTATTGCGCGTTTAATTACAGAGTTTTTCTTGCTCATTTGGCGATTTATTGAT
ATTACTAAAATCAATGTGAAGTTGAATATTGTAAGTACGATTCAATGTGTCATTGCTGCCGTTATGATGTTTATTGTGCT
TGGTGTGGTCAATCATTATTTGCCCCCTACAATGTACGCTACGCTGCTATTAATTGCGATTGGTATAGTAGTTTATCTTT
TATTAATGATGACTATGAAAAATCAATACGTATGGCAAATATTGAGGCATCTTCGACATAAAACAATTTAA

Upstream 100 bases:

>100_bases
TACTTTTTAACAGTATCGTTTAATAACTCAAGATATGTCGCTTTTATTTTAGGAATTATCGTCTTTATTGTTCAATATGA
AAAGATGGAAAGGGATCGTA

Downstream 100 bases:

>100_bases
GTATCGGTAACGCTATACTTTAGAAATTTAAGATGAAGAAGAAAAGGCAATTTCTTATTGAAAAATGGAAGTTGTCTTTT
TTAATTCTTTTTAAAAGCGG

Product: lipopolysaccharide flipase protein

Products: lipopolysaccharide [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MKSDSLKENIIYQGLYQLIRTMTPLITIPIISRAFGPSGVGIVSFSFNIVQYFLMIASVGVQLYFNRVIAKSVNDKRQLS
QQFWDIFVSKLLLSLTVFAIYIAVITIFIDDYYLIFLLQGIYIIGAALDISWFYAGTEKFKIPSLSNIVASGIVLSVVVI
FVKDQSDLSLYVFTIAIVTVLNQLPLFIYLKRYISFVSVNWIHVWQLFRSSLAYLLPNGQLNLYTSISCVVLGLVGTYQQ
VGTFSNAFNILTVAIIMINTFDLVMIPRITKMSIQKSHSLTKTLANNMNIQLILTVPMVFGLIAIMPSFYLWFFGEEFAS
TVPLMTILAILVLIIPLNMLISRQYLLIVNKIRLYNASITIGAVMNLVLCLVLIYFYGIYGAAIARLITEFFLLIWRFID
ITKINVKLNIVSTIQCVIAAVMMFIVLGVVNHYLPPTMYATLLLIAIGIVVYLLLMMTMKNQYVWQILRHLRHKTI

Sequences:

>Translated_476_residues
MKSDSLKENIIYQGLYQLIRTMTPLITIPIISRAFGPSGVGIVSFSFNIVQYFLMIASVGVQLYFNRVIAKSVNDKRQLS
QQFWDIFVSKLLLSLTVFAIYIAVITIFIDDYYLIFLLQGIYIIGAALDISWFYAGTEKFKIPSLSNIVASGIVLSVVVI
FVKDQSDLSLYVFTIAIVTVLNQLPLFIYLKRYISFVSVNWIHVWQLFRSSLAYLLPNGQLNLYTSISCVVLGLVGTYQQ
VGTFSNAFNILTVAIIMINTFDLVMIPRITKMSIQKSHSLTKTLANNMNIQLILTVPMVFGLIAIMPSFYLWFFGEEFAS
TVPLMTILAILVLIIPLNMLISRQYLLIVNKIRLYNASITIGAVMNLVLCLVLIYFYGIYGAAIARLITEFFLLIWRFID
ITKINVKLNIVSTIQCVIAAVMMFIVLGVVNHYLPPTMYATLLLIAIGIVVYLLLMMTMKNQYVWQILRHLRHKTI
>Mature_476_residues
MKSDSLKENIIYQGLYQLIRTMTPLITIPIISRAFGPSGVGIVSFSFNIVQYFLMIASVGVQLYFNRVIAKSVNDKRQLS
QQFWDIFVSKLLLSLTVFAIYIAVITIFIDDYYLIFLLQGIYIIGAALDISWFYAGTEKFKIPSLSNIVASGIVLSVVVI
FVKDQSDLSLYVFTIAIVTVLNQLPLFIYLKRYISFVSVNWIHVWQLFRSSLAYLLPNGQLNLYTSISCVVLGLVGTYQQ
VGTFSNAFNILTVAIIMINTFDLVMIPRITKMSIQKSHSLTKTLANNMNIQLILTVPMVFGLIAIMPSFYLWFFGEEFAS
TVPLMTILAILVLIIPLNMLISRQYLLIVNKIRLYNASITIGAVMNLVLCLVLIYFYGIYGAAIARLITEFFLLIWRFID
ITKINVKLNIVSTIQCVIAAVMMFIVLGVVNHYLPPTMYATLLLIAIGIVVYLLLMMTMKNQYVWQILRHLRHKTI

Specific function: May be involved in the translocation process of the nascent O-polysaccharide molecules and/or its ligation to lipid A core units [H]

COG id: COG2244

COG function: function code R; Membrane protein involved in the export of O-antigen and teichoic acid

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788349, Length=273, Percent_Identity=23.8095238095238, Blast_Score=83, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002797 [H]

Pfam domain/function: PF01943 Polysacc_synt [H]

EC number: NA

Molecular weight: Translated: 54058; Mature: 54058

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSDSLKENIIYQGLYQLIRTMTPLITIPIISRAFGPSGVGIVSFSFNIVQYFLMIASVG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHH
VQLYFNRVIAKSVNDKRQLSQQFWDIFVSKLLLSLTVFAIYIAVITIFIDDYYLIFLLQG
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IYIIGAALDISWFYAGTEKFKIPSLSNIVASGIVLSVVVIFVKDQSDLSLYVFTIAIVTV
HHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHH
LNQLPLFIYLKRYISFVSVNWIHVWQLFRSSLAYLLPNGQLNLYTSISCVVLGLVGTYQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHH
VGTFSNAFNILTVAIIMINTFDLVMIPRITKMSIQKSHSLTKTLANNMNIQLILTVPMVF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHH
GLIAIMPSFYLWFFGEEFASTVPLMTILAILVLIIPLNMLISRQYLLIVNKIRLYNASIT
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEECCCH
IGAVMNLVLCLVLIYFYGIYGAAIARLITEFFLLIWRFIDITKINVKLNIVSTIQCVIAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEHHHHHHHHHHH
VMMFIVLGVVNHYLPPTMYATLLLIAIGIVVYLLLMMTMKNQYVWQILRHLRHKTI
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKSDSLKENIIYQGLYQLIRTMTPLITIPIISRAFGPSGVGIVSFSFNIVQYFLMIASVG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHH
VQLYFNRVIAKSVNDKRQLSQQFWDIFVSKLLLSLTVFAIYIAVITIFIDDYYLIFLLQG
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IYIIGAALDISWFYAGTEKFKIPSLSNIVASGIVLSVVVIFVKDQSDLSLYVFTIAIVTV
HHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHH
LNQLPLFIYLKRYISFVSVNWIHVWQLFRSSLAYLLPNGQLNLYTSISCVVLGLVGTYQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHH
VGTFSNAFNILTVAIIMINTFDLVMIPRITKMSIQKSHSLTKTLANNMNIQLILTVPMVF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHH
GLIAIMPSFYLWFFGEEFASTVPLMTILAILVLIIPLNMLISRQYLLIVNKIRLYNASIT
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEECCCH
IGAVMNLVLCLVLIYFYGIYGAAIARLITEFFLLIWRFIDITKINVKLNIVSTIQCVIAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEHHHHHHHHHHH
VMMFIVLGVVNHYLPPTMYATLLLIAIGIVVYLLLMMTMKNQYVWQILRHLRHKTI
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lipopolysaccharide [Cytoplasm] [C]

Specific reaction: lipopolysaccharide [Cytoplasm] = lipopolysaccharide [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7517390; 7517391; 9097040; 9278503 [H]