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The map label for this gene is rpoH [H]

Identifier: 82703854

GI number: 82703854

Start: 3146280

End: 3147125

Strand: Direct

Name: rpoH [H]

Synonym: Nmul_A2741

Alternate gene names: 82703854

Gene position: 3146280-3147125 (Clockwise)

Preceding gene: 82703853

Following gene: 82703869

Centisome position: 98.81

GC content: 57.57

Gene sequence:

>846_bases
ATGACTTTCGCTTTAACCGTACCTTCTGCCGGCAGCCTCGAAAACTACATTCAGTCCGTCAACCAGTTCCGCATCCTTAC
TCAGGAAGAGGAACTGCAACTGGCCCGGTCGCTGCGTGATGAGGGCAGCATAGATGCTGCGCGCCAGCTCGTGCTGTCGC
ACCTGCGCCTGGTCGTCGCCATCGCGCGGGGTTACAAAGGCTATGGCTTGCCTCAGGCTGACCTGATACAGGAAGGAAAT
ATCGGATTGATGAAAGCGGTCAAGCGCTTTGACCCCGAGCGCGGCGTCCGGCTGGTTTCATTTGCAGTACACTGGATCAA
GGCTGAAATCCACGAATTCATACTGCGCAACTGGCGCCTGGTTAAAATTGCCACGACAAAGGCTCAGCGCAAGCTATTTT
TCAATTTACGCAGCTTGAAACAGGCTCTCGATACCATGAGCCAGAAGGAAGTTGCCGAGGTTGCAGAACAGCTGGGTGTA
AAGCCCGAGGAAGTGGTGGAAATGGAAACCCGCTTCAGCGGCCGCGATATTTCACTCGAACCCCTGTCGGAAGACGACGA
TGAGGAAACCTTCAGCCCCATTGCTTACCTGACCGATGGTTCCGAACCCTCCCATGTGCTGGAGGACGAGCAAACCATGC
GTCTGCGGGGTGAAGGACTGGAGCAGGCGCTCGAGAGCCTGGATGCTCGCAGCCGCCACATCATCGAGGCCCGCTGGCTG
CGGGAAAAAGACACTGCGACGCTGCATGAGCTCGCCGATGAGTTGGGAGTATCCGCCGAGCGTGTCCGCCAGATCGAAGC
CAAGGCCATGCAGAAGCTGCGTGCGGTCATGGCGCCTGCCGCCTGA

Upstream 100 bases:

>100_bases
AGGTTAAGGTACTTTACAATGAATAGTGGAGGGAACTTTCAAAAATGTTGACGCTCTTAAAGGGAAACGGTTAACGTTCA
CAGGAAACCAAGGAGGATAA

Downstream 100 bases:

>100_bases
TACCCCGGCGGAGTCTCTGTTCTCGAATCCAGATTGCGCAGCCCTAGTCCCCAAGGGGAGCTCAGTTCTGGTTGTTGCTT
TCCGCCCACTGCTCCGGCGT

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MTFALTVPSAGSLENYIQSVNQFRILTQEEELQLARSLRDEGSIDAARQLVLSHLRLVVAIARGYKGYGLPQADLIQEGN
IGLMKAVKRFDPERGVRLVSFAVHWIKAEIHEFILRNWRLVKIATTKAQRKLFFNLRSLKQALDTMSQKEVAEVAEQLGV
KPEEVVEMETRFSGRDISLEPLSEDDDEETFSPIAYLTDGSEPSHVLEDEQTMRLRGEGLEQALESLDARSRHIIEARWL
REKDTATLHELADELGVSAERVRQIEAKAMQKLRAVMAPAA

Sequences:

>Translated_281_residues
MTFALTVPSAGSLENYIQSVNQFRILTQEEELQLARSLRDEGSIDAARQLVLSHLRLVVAIARGYKGYGLPQADLIQEGN
IGLMKAVKRFDPERGVRLVSFAVHWIKAEIHEFILRNWRLVKIATTKAQRKLFFNLRSLKQALDTMSQKEVAEVAEQLGV
KPEEVVEMETRFSGRDISLEPLSEDDDEETFSPIAYLTDGSEPSHVLEDEQTMRLRGEGLEQALESLDARSRHIIEARWL
REKDTATLHELADELGVSAERVRQIEAKAMQKLRAVMAPAA
>Mature_280_residues
TFALTVPSAGSLENYIQSVNQFRILTQEEELQLARSLRDEGSIDAARQLVLSHLRLVVAIARGYKGYGLPQADLIQEGNI
GLMKAVKRFDPERGVRLVSFAVHWIKAEIHEFILRNWRLVKIATTKAQRKLFFNLRSLKQALDTMSQKEVAEVAEQLGVK
PEEVVEMETRFSGRDISLEPLSEDDDEETFSPIAYLTDGSEPSHVLEDEQTMRLRGEGLEQALESLDARSRHIIEARWLR
EKDTATLHELADELGVSAERVRQIEAKAMQKLRAVMAPAA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=276, Percent_Identity=55.0724637681159, Blast_Score=292, Evalue=1e-80,
Organism=Escherichia coli, GI1789098, Length=267, Percent_Identity=33.7078651685393, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789448, Length=234, Percent_Identity=33.3333333333333, Blast_Score=105, Evalue=4e-24,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 31792; Mature: 31661

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFALTVPSAGSLENYIQSVNQFRILTQEEELQLARSLRDEGSIDAARQLVLSHLRLVVA
CEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
IARGYKGYGLPQADLIQEGNIGLMKAVKRFDPERGVRLVSFAVHWIKAEIHEFILRNWRL
HHHCCCCCCCCHHHHHCCCCHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHHHHHCCEE
VKIATTKAQRKLFFNLRSLKQALDTMSQKEVAEVAEQLGVKPEEVVEMETRFSGRDISLE
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEC
PLSEDDDEETFSPIAYLTDGSEPSHVLEDEQTMRLRGEGLEQALESLDARSRHIIEARWL
CCCCCCCCHHHCCEEEEECCCCCCHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
REKDTATLHELADELGVSAERVRQIEAKAMQKLRAVMAPAA
HCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TFALTVPSAGSLENYIQSVNQFRILTQEEELQLARSLRDEGSIDAARQLVLSHLRLVVA
EEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
IARGYKGYGLPQADLIQEGNIGLMKAVKRFDPERGVRLVSFAVHWIKAEIHEFILRNWRL
HHHCCCCCCCCHHHHHCCCCHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHHHHHCCEE
VKIATTKAQRKLFFNLRSLKQALDTMSQKEVAEVAEQLGVKPEEVVEMETRFSGRDISLE
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEC
PLSEDDDEETFSPIAYLTDGSEPSHVLEDEQTMRLRGEGLEQALESLDARSRHIIEARWL
CCCCCCCCHHHCCEEEEECCCCCCHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
REKDTATLHELADELGVSAERVRQIEAKAMQKLRAVMAPAA
HCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]