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The map label for this gene is dadA [C]

Identifier: 82703836

GI number: 82703836

Start: 3122591

End: 3123727

Strand: Reverse

Name: dadA [C]

Synonym: Nmul_A2723

Alternate gene names: 82703836

Gene position: 3123727-3122591 (Counterclockwise)

Preceding gene: 82703837

Following gene: 82703835

Centisome position: 98.1

GC content: 58.84

Gene sequence:

>1137_bases
TTGAGCAGCGATGTGATGGTGATGGGTGGCGGCATCGTCGGGCTTGCCACCGCGTGGGAATTATTGCGTTCGGGCGCGAC
AGTTTCCGTACTTGAGCGCAATCTTTGCGGGCAGGAAGCCTCCTGGGCGGGCGCCGGCATACTCTCGCCGTTGCTGCCAT
GGGACTATCCGGAAGCGGTTACACAGCTTACACAGTTTAGTGCTCATCTGTTTCCTCGATTTGTTGATGCGTTGCAGGCG
GAAACGGGCGTCGATCCGGAATATCAGGCAAGCGGCATGCTGGTGCTGCCCGATTCTTCCTCCCAGGCCTATGTCGAACC
GGGGACGTTTGATATGTGCAGCCGGATGCTTCTGGCCAAGCACTGGTGCGCGCGTCACGCCATACCCGTGCAGGAGGTTC
AGTCCAGCCAAATAGCCCCTTCGCTCTCCCGTGACACGGCTGCGCTCTGGCTGCCCGAAGTATGCCAGGTAAGAAATCCA
CGTTTGCTGCAGGCGTTGATAAGGGCGGTGAAGATGCTTGGCGGTAAAGTCCTCGAGCATGCCGAGGTTGCCGGGTGGAA
AATCGAACGGGGATACGTCCAATCCATCCATACCGTGGCGGGCGAGCGTCATTCCGCAGGTCAATATGTTGTTGCCGCGG
GGGCATGGAGCAGGGGGCTTCTGGCTGAGCATGCGGCAGCGCTGGAAGCTTGGCCGGTCCGCGGCCAGATTCTGTTGTTC
AAGGCCCAGCCAGGATTGCTCGAGCCCATGGTGTTCGATGAGCGGGACAATTTTTATCTCATCCCCCGCCGGGATGGACA
CATACTTGCAGGCAGCACCCTGGAGGAAGCAGGTTTCGACAAGAGTACAACGCCCGAAGCGCGAGAAACATTGCTTGCAA
GGGCGCAAGCCCTGATTCCTGTTCTTGCTGAAGAGATGATTGCCGCGCATTGGGCAGGCCTGCGGCCCGCTTCACCTCAT
AACATACCTGTGATTTCCATGCATCCGGCGATCTCCAACCTGTATCTCAACAGCGGTCATTATCGCTACGGTGTCACCAT
GGCCCCCGGCAGTGCGCAGCTTATCGCCAACATGATTCTGGGCAAGCTCCAGCCGCTCGATGTGACGCCCTACCAGTGGC
CGCATAGGGCGATTTGA

Upstream 100 bases:

>100_bases
CTTGTAGAACTCGTCGGTTTTGTAAACAGCTGCTCGAATTGCGTTTCATCACAGAACCGCTTTTGTTATTCTGTGCGGAG
GCTTGTTGAAGGAATAATTT

Downstream 100 bases:

>100_bases
ACCGGTAAGTTTGAAACCAGTAAGTCCGATTATCGACCAGGGAGGGCCGCTTGCGGAACGCCTCCTTGTCGGCTTTATGC
TGGAGACGGGGGGTGATAAC

Product: FAD dependent oxidoreductase

Products: FADH2; ammonia; Pyruvate [C]

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MSSDVMVMGGGIVGLATAWELLRSGATVSVLERNLCGQEASWAGAGILSPLLPWDYPEAVTQLTQFSAHLFPRFVDALQA
ETGVDPEYQASGMLVLPDSSSQAYVEPGTFDMCSRMLLAKHWCARHAIPVQEVQSSQIAPSLSRDTAALWLPEVCQVRNP
RLLQALIRAVKMLGGKVLEHAEVAGWKIERGYVQSIHTVAGERHSAGQYVVAAGAWSRGLLAEHAAALEAWPVRGQILLF
KAQPGLLEPMVFDERDNFYLIPRRDGHILAGSTLEEAGFDKSTTPEARETLLARAQALIPVLAEEMIAAHWAGLRPASPH
NIPVISMHPAISNLYLNSGHYRYGVTMAPGSAQLIANMILGKLQPLDVTPYQWPHRAI

Sequences:

>Translated_378_residues
MSSDVMVMGGGIVGLATAWELLRSGATVSVLERNLCGQEASWAGAGILSPLLPWDYPEAVTQLTQFSAHLFPRFVDALQA
ETGVDPEYQASGMLVLPDSSSQAYVEPGTFDMCSRMLLAKHWCARHAIPVQEVQSSQIAPSLSRDTAALWLPEVCQVRNP
RLLQALIRAVKMLGGKVLEHAEVAGWKIERGYVQSIHTVAGERHSAGQYVVAAGAWSRGLLAEHAAALEAWPVRGQILLF
KAQPGLLEPMVFDERDNFYLIPRRDGHILAGSTLEEAGFDKSTTPEARETLLARAQALIPVLAEEMIAAHWAGLRPASPH
NIPVISMHPAISNLYLNSGHYRYGVTMAPGSAQLIANMILGKLQPLDVTPYQWPHRAI
>Mature_377_residues
SSDVMVMGGGIVGLATAWELLRSGATVSVLERNLCGQEASWAGAGILSPLLPWDYPEAVTQLTQFSAHLFPRFVDALQAE
TGVDPEYQASGMLVLPDSSSQAYVEPGTFDMCSRMLLAKHWCARHAIPVQEVQSSQIAPSLSRDTAALWLPEVCQVRNPR
LLQALIRAVKMLGGKVLEHAEVAGWKIERGYVQSIHTVAGERHSAGQYVVAAGAWSRGLLAEHAAALEAWPVRGQILLFK
AQPGLLEPMVFDERDNFYLIPRRDGHILAGSTLEEAGFDKSTTPEARETLLARAQALIPVLAEEMIAAHWAGLRPASPHN
IPVISMHPAISNLYLNSGHYRYGVTMAPGSAQLIANMILGKLQPLDVTPYQWPHRAI

Specific function: Oxidative Deamination Of D-Amino Acids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=417, Percent_Identity=26.8585131894484, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI28571104, Length=393, Percent_Identity=26.4631043256997, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076
- InterPro:   IPR012727 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.4.99.1 [C]

Molecular weight: Translated: 41158; Mature: 41027

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSDVMVMGGGIVGLATAWELLRSGATVSVLERNLCGQEASWAGAGILSPLLPWDYPEAV
CCCCEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHCCCCCCHHHH
TQLTQFSAHLFPRFVDALQAETGVDPEYQASGMLVLPDSSSQAYVEPGTFDMCSRMLLAK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCEECCCCHHHHHHHHHHH
HWCARHAIPVQEVQSSQIAPSLSRDTAALWLPEVCQVRNPRLLQALIRAVKMLGGKVLEH
HHHHHCCCCHHHHHHHHCCCCCCCCHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
AEVAGWKIERGYVQSIHTVAGERHSAGQYVVAAGAWSRGLLAEHAAALEAWPVRGQILLF
HHHCCEEECHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEE
KAQPGLLEPMVFDERDNFYLIPRRDGHILAGSTLEEAGFDKSTTPEARETLLARAQALIP
ECCCCCCCCEEECCCCCEEEEECCCCCEEECCCHHHCCCCCCCCHHHHHHHHHHHHHHHH
VLAEEMIAAHWAGLRPASPHNIPVISMHPAISNLYLNSGHYRYGVTMAPGSAQLIANMIL
HHHHHHHHHHHCCCCCCCCCCCCEEEECCCHHHEEECCCCEEEEEEECCCHHHHHHHHHH
GKLQPLDVTPYQWPHRAI
HCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SSDVMVMGGGIVGLATAWELLRSGATVSVLERNLCGQEASWAGAGILSPLLPWDYPEAV
CCCEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHCCCCCCHHHH
TQLTQFSAHLFPRFVDALQAETGVDPEYQASGMLVLPDSSSQAYVEPGTFDMCSRMLLAK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCEECCCCHHHHHHHHHHH
HWCARHAIPVQEVQSSQIAPSLSRDTAALWLPEVCQVRNPRLLQALIRAVKMLGGKVLEH
HHHHHCCCCHHHHHHHHCCCCCCCCHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
AEVAGWKIERGYVQSIHTVAGERHSAGQYVVAAGAWSRGLLAEHAAALEAWPVRGQILLF
HHHCCEEECHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEE
KAQPGLLEPMVFDERDNFYLIPRRDGHILAGSTLEEAGFDKSTTPEARETLLARAQALIP
ECCCCCCCCEEECCCCCEEEEECCCCCEEECCCHHHCCCCCCCCHHHHHHHHHHHHHHHH
VLAEEMIAAHWAGLRPASPHNIPVISMHPAISNLYLNSGHYRYGVTMAPGSAQLIANMIL
HHHHHHHHHHHCCCCCCCCCCCCEEEECCCHHHEEECCCCEEEEEEECCCHHHHHHHHHH
GKLQPLDVTPYQWPHRAI
HCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {D-proline}} 0.13 {K3Fe(CN)6}} 0.63 {dichloroindophenol}} 0.01 {dichloroindophenol}} 11 {D-methionine}} 6 {D-phenylalanine}} 12 {D-alpha-aminobutyrate}} 0.15 {cytochrome} 19 {D-serine}} 29 {D-alanine}} 6.4 {D-alanine}} 5.3 {D

Substrates: D-Alanine; FAD; H2O [C]

Specific reaction: D-Alanine + FAD + H2O --> FADH2 + ammonia + Pyruvate [C]

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8682785; 10984043; 8097014 [H]