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The map label for this gene is ribF [H]

Identifier: 82703766

GI number: 82703766

Start: 3043677

End: 3044684

Strand: Direct

Name: ribF [H]

Synonym: Nmul_A2653

Alternate gene names: 82703766

Gene position: 3043677-3044684 (Clockwise)

Preceding gene: 82703765

Following gene: 82703767

Centisome position: 95.59

GC content: 57.94

Gene sequence:

>1008_bases
ATGCGCATTTCCCACGGCACTCCCGTTCAGGCTGATGCGCCGGTTGCCCTCACCATCGGCAATTTCGATGGGGTGCATCT
GGGGCACCAGGCGATGCTTGCGCGCCTGAAAAAGGCAGCTGACCGGCTTGGTGTCGAATCCTGTGTCATGATTTTCGAAC
CGCATCCGCGTGAATTTTTCGCACCGGATAAGGCGCCCACGCGGCTCACCAGCCTGCGGGAGAAGCTGGAGTTGCTGGCG
GCAGCCGGAGTGGAGCGGGTGCAGATATGCCGCTTCGATTTCGATTTTGCCAGAATTCCGGCGGAGGATTTTATTGTCCG
CATCCTTCAGCATGGTCTGGCTGCGCGCTGGATCCTGGTGGGAGACGACTTTCGCTTCGGTGCACGCCGTGCCGGTGATT
ATGAGATGCTCAAGGCATTTTCGGCAGAGTGCGGTTTCGAGGTGGAGGACATGCCGGGCTTTACCGTAAACGGCCTGCGG
GTTTCAAGCACGGCGGTTCGCGAAGCATTGGCGGCCGGCGATCTCGACCTGGTCAAACGCCTGCTGGGCCGTTTCTACAG
CATCAGCGGGCGCGTGGTGGATGGCGACAAGCTGGGCAAGAAGATCGGGTTTCCTACCGCGAACATCCAGCTCAAGCATA
ATCGCCCGCCGCTGGCGGGAATCTTTGCCGTTGAAGTCGAAATGGAAGCTTCGGGTAACGAGACGCCGCCGTCATTGGAA
GTGCCTTCATTGGATACACTGCGGGGGGTTGCAAGCCTGGGGGTGCGTCCTACCGTGCATGAGCATGGCAAGCCGGTGCT
GGAGGTTCACCTGTTCGATTTCGACCAGGAAATTTATGGCCGCCATTTGCGGGTGCATTTTCTTCACAAGCTGCGTGATG
AGGAAAAATATTCCGGCCTCGAGGCGCTGACCAGACAGATTGGCCGGGATGTGGACAATGCAAAGAATTACTTCTCCTCG
TTATCCGCAGCGCCCGCTCTTTCTGAAAAGAAGCTTGTGCAAGGATAA

Upstream 100 bases:

>100_bases
AATTTCTTGCTCCCACACATCGGAGCATTAACCGCCTGAAAATCAGCTGCCTGAAACTCGTCCAAACAGGCTACAATTCA
GCATTTTAGCAGCAATAGGC

Downstream 100 bases:

>100_bases
GGCTGCCATTGCTGCCATTCGCTACTTTTTCGATATTTCATGACCGACTATAAGAAAACGCTGAACCTGCCCGATACGCC
TTTTCCGATGCGCGGCGATC

Product: riboflavin biosynthesis protein RibF

Products: NA

Alternate protein names: Riboflavin kinase; Flavokinase; FMN adenylyltransferase; FAD pyrophosphorylase; FAD synthase [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MRISHGTPVQADAPVALTIGNFDGVHLGHQAMLARLKKAADRLGVESCVMIFEPHPREFFAPDKAPTRLTSLREKLELLA
AAGVERVQICRFDFDFARIPAEDFIVRILQHGLAARWILVGDDFRFGARRAGDYEMLKAFSAECGFEVEDMPGFTVNGLR
VSSTAVREALAAGDLDLVKRLLGRFYSISGRVVDGDKLGKKIGFPTANIQLKHNRPPLAGIFAVEVEMEASGNETPPSLE
VPSLDTLRGVASLGVRPTVHEHGKPVLEVHLFDFDQEIYGRHLRVHFLHKLRDEEKYSGLEALTRQIGRDVDNAKNYFSS
LSAAPALSEKKLVQG

Sequences:

>Translated_335_residues
MRISHGTPVQADAPVALTIGNFDGVHLGHQAMLARLKKAADRLGVESCVMIFEPHPREFFAPDKAPTRLTSLREKLELLA
AAGVERVQICRFDFDFARIPAEDFIVRILQHGLAARWILVGDDFRFGARRAGDYEMLKAFSAECGFEVEDMPGFTVNGLR
VSSTAVREALAAGDLDLVKRLLGRFYSISGRVVDGDKLGKKIGFPTANIQLKHNRPPLAGIFAVEVEMEASGNETPPSLE
VPSLDTLRGVASLGVRPTVHEHGKPVLEVHLFDFDQEIYGRHLRVHFLHKLRDEEKYSGLEALTRQIGRDVDNAKNYFSS
LSAAPALSEKKLVQG
>Mature_335_residues
MRISHGTPVQADAPVALTIGNFDGVHLGHQAMLARLKKAADRLGVESCVMIFEPHPREFFAPDKAPTRLTSLREKLELLA
AAGVERVQICRFDFDFARIPAEDFIVRILQHGLAARWILVGDDFRFGARRAGDYEMLKAFSAECGFEVEDMPGFTVNGLR
VSSTAVREALAAGDLDLVKRLLGRFYSISGRVVDGDKLGKKIGFPTANIQLKHNRPPLAGIFAVEVEMEASGNETPPSLE
VPSLDTLRGVASLGVRPTVHEHGKPVLEVHLFDFDQEIYGRHLRVHFLHKLRDEEKYSGLEALTRQIGRDVDNAKNYFSS
LSAAPALSEKKLVQG

Specific function: Unknown

COG id: COG0196

COG function: function code H; FAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribF family [H]

Homologues:

Organism=Escherichia coli, GI1786208, Length=321, Percent_Identity=45.1713395638629, Blast_Score=256, Evalue=2e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004821
- InterPro:   IPR015864
- InterPro:   IPR015865
- InterPro:   IPR002606
- InterPro:   IPR014729 [H]

Pfam domain/function: PF06574 FAD_syn; PF01687 Flavokinase [H]

EC number: =2.7.1.26; =2.7.7.2 [H]

Molecular weight: Translated: 37019; Mature: 37019

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRISHGTPVQADAPVALTIGNFDGVHLGHQAMLARLKKAADRLGVESCVMIFEPHPREFF
CCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHEEEEECCCCCCCC
APDKAPTRLTSLREKLELLAAAGVERVQICRFDFDFARIPAEDFIVRILQHGLAARWILV
CCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHCCHHHHHHHHHHHCCCEEEEEE
GDDFRFGARRAGDYEMLKAFSAECGFEVEDMPGFTVNGLRVSSTAVREALAAGDLDLVKR
CCCCCCCCCCCCCHHHHHHHHHHCCCEECCCCCCEECCEEECHHHHHHHHHCCCHHHHHH
LLGRFYSISGRVVDGDKLGKKIGFPTANIQLKHNRPPLAGIFAVEVEMEASGNETPPSLE
HHHHHHHCCCEEECHHHHHHHHCCCCCEEEEECCCCCEEEEEEEEEEEECCCCCCCCCCC
VPSLDTLRGVASLGVRPTVHEHGKPVLEVHLFDFDQEIYGRHLRVHFLHKLRDEEKYSGL
CCCHHHHHHHHHCCCCCCHHHCCCCEEEEEEECCCHHHHCCHHHHHHHHHHHCHHHHHHH
EALTRQIGRDVDNAKNYFSSLSAAPALSEKKLVQG
HHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHCCC
>Mature Secondary Structure
MRISHGTPVQADAPVALTIGNFDGVHLGHQAMLARLKKAADRLGVESCVMIFEPHPREFF
CCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHEEEEECCCCCCCC
APDKAPTRLTSLREKLELLAAAGVERVQICRFDFDFARIPAEDFIVRILQHGLAARWILV
CCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHCCHHHHHHHHHHHCCCEEEEEE
GDDFRFGARRAGDYEMLKAFSAECGFEVEDMPGFTVNGLRVSSTAVREALAAGDLDLVKR
CCCCCCCCCCCCCHHHHHHHHHHCCCEECCCCCCEECCEEECHHHHHHHHHCCCHHHHHH
LLGRFYSISGRVVDGDKLGKKIGFPTANIQLKHNRPPLAGIFAVEVEMEASGNETPPSLE
HHHHHHHCCCEEECHHHHHHHHCCCCCEEEEECCCCCEEEEEEEEEEEECCCCCCCCCCC
VPSLDTLRGVASLGVRPTVHEHGKPVLEVHLFDFDQEIYGRHLRVHFLHKLRDEEKYSGL
CCCHHHHHHHHHCCCCCCHHHCCCCEEEEEEECCCHHHHCCHHHHHHHHHHHCHHHHHHH
EALTRQIGRDVDNAKNYFSSLSAAPALSEKKLVQG
HHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]