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The map label for this gene is metE [H]

Identifier: 82703747

GI number: 82703747

Start: 3018450

End: 3020840

Strand: Reverse

Name: metE [H]

Synonym: Nmul_A2634

Alternate gene names: 82703747

Gene position: 3020840-3018450 (Counterclockwise)

Preceding gene: 82703749

Following gene: 82703743

Centisome position: 94.87

GC content: 57.09

Gene sequence:

>2391_bases
ATGGCACTGACACATAACCTGGGATTTCCGCGTATTGGCGCGCAACGGGAACTGAAGCGCGCGCTTGAGGCATACTGGCG
CGGCGATATCGGTGATGAGCAACTCAAAACCACGGCAGCGGAATTGAGGAAGCGGCACTGGCTTTTGCAGCGCGAGCAGG
GTATTGATCTCATTCCCGCAGGCGATTTCGCCCTTTATGACCAGATGCTGAACATGACCGCACTGCTGAGTGCAATACCT
GCACGCTTCGGAGCAGACAAGGGTAAAACAGAGCTGGACTTGTACTTCGCCATGGCGCGTGGCACTGTCGATCAGCCTGC
GATGGAGATGACCAAATGGTTCGACACCAACTATCACTATATCGTCCCGGAATTCGATGCCCACACCTGCTTTCGTATAG
GTTCCGAGCAGCTCTTCAGGGAGGTTGCCGAAGTGCAGGCGCTCGGCATCAAGCCTAAAGCCGTCTTGATCGGGCCGTTG
ACCTACCTGTATCTTGGAAAAGAGGTCACCCGGCAGAATGAGCAGGAATTCAGCCGCCTGGATTTGTTACCCCAACTGTT
ACCGGTATATCGCGATATTCTCGTCCGGCTTGCGTCGCTAGGCGTGGAATGGGTACAGATCGACGAACCTGTGCTGGCCC
TCGACCTGGACGAGGACTGGCTGCGGGGTTTGGATCAGGCTTATGCCGGTTTGCGGGAACCGGCGGATGGACAGGCCCAC
GGCCCCTCGGCACTTGCCCGGCTCCCTAAAATTCTTTTAGCGACTTATTTCGATGGCGTGAACAATCATGCCGAGCGATT
GAAGAAGCTACCCGTGGAAGGCCTCCATATCGATTTGTGCCGGGCGCCTGGTCAACTCGGTATTTTTCTGGAGGGCTACC
CGGCCGGAAGAGTGCTGTCCCTGGGCATTATCGATGGGCGTAACATCTGGCGGGCAGACCTTGCTCCCACCTTGGCGGTT
TTGTCGCATGCCCAGGCCCTCCTCGGGGAAAGACTGTGGATTGCGCCGAGTTGCTCGCTTCTGCATTGTCCGGTGGATCT
GGAACTGGAAAGCAGTCTGGAGCCTGAAATAAGGAACTGGCTGGCTTTCTCGGTGCAGAAATTAAAAGAAGTCTCCATCC
TGGGGCAAGGGCTGAATCAGGGGGAACATTCTATACGCCAGCAATTGGATGCTTCCACCGCAGCGAGGCAGGCTCGGCGG
GAGTCATCGCGCATCCACAACCCGGTGGTGCAGGACCGTCTGAATCGCCTGACTGAACAGGATGGCCGGCGCAAGAGTCC
GTTCAAGGTCAGGCAGAAACTGCAACGGGAGCGGCTACAGCTTCCGCTTTTGCCTACTACAACCATCGGGTCATTTCCTC
AGACAGCGGAGATCCGCAAAGCGCGCGCGGCTTTCAGGAAAGGAGAGCTCGGCAATTTGCAATATCTTGAAGCGATGCGG
GACGAGATACGAGTGGCGATCAGAAAGCAGGAAGAAATCGGGCTCGATGTACTGGTGCATGGCGAGGCCGAGCGCAACGA
TATGGTCGAGTATTTTGGCGAACAGCTATGGGGTTATGTCTTCACGGAGAATGGATGGGTGCAAAGCTACGGCTCGCGCT
GCGTGAAACCGCCGATTCTCTACGGGGATATTTACCGTCCCGAGCCCATGACGGCTGACTGGATTCGATATGCGCAAAGC
CAGACGGGAAAGCCGGTCAAGGGGATGCTGACCGGTCCCGTTACAATGTTGATGTGGTCGTTCGTGCGTGATGACCAGCC
GCGCTCCGTCACCGCGCTGCAGCTCGCCCTTGCCATTCGGGACGAGGTTGTGGATCTGGAAGAGGCGGGCATTGCGATTA
TCCAGATCGACGAGCCCGCTTTCCGCGAGGGCCTGCCCTTGAAAAAGCGAGATTGGAATGCATACCTGGAATGGGCGGTG
CATGCCTTCCGTGTCGCCAGCTCGGGCGTAAGGGATGACAGCCAGATACACACCCATATGTGCTACTCGGAATTCAATGA
CATCCTTCCCGCCATTGCCGATATGGATGCAGATGTAATCACGATCGAAACGTCACGTTCACGCATGGAGCTGCTGGACG
GATTCGGCCATTTCAAATATCCCAACGAGATCGGGCCGGGCGTCTATGACATCCACTCCCCGCGGATTCCCCGTTCGCGG
GAGATGCTGGAGTTGCTGGAGAAAGCCTGCGGGGTGATCGACCCTGACCAATTATGGGTCAACCCGGATTGCGGGCTGAA
GACGCGGGGATGGGGGGAAGTCATGACTGCCTTGAAGCAAATGGTGGAAGCCGCACACCTGTTACGGGCCCGTCTCGGGT
ATCGGGAAGACGAGTCGCAGCTTGAGGCACCTGAATCCGATAAGGAACCCGCTCGAGTCAGCGCGGGATAA

Upstream 100 bases:

>100_bases
CAACATTTCATTTGATTCATAACCAAGTCTGCCACAAAATCGTCAGTCACTCCACAGAGCTGCAATTGAAGTGGGACCAA
ACGATAGAGGAGAATGACCA

Downstream 100 bases:

>100_bases
TCCGGTCTCCGGCAGAAGGTTTTCCGGGGGCTTTTCCGGGTGTCTCCGGTTGCGGGGATGTGTGCAGCACCTTTATCAGG
TTTGCTTCGAGCTGGATCAC

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]

Number of amino acids: Translated: 796; Mature: 795

Protein sequence:

>796_residues
MALTHNLGFPRIGAQRELKRALEAYWRGDIGDEQLKTTAAELRKRHWLLQREQGIDLIPAGDFALYDQMLNMTALLSAIP
ARFGADKGKTELDLYFAMARGTVDQPAMEMTKWFDTNYHYIVPEFDAHTCFRIGSEQLFREVAEVQALGIKPKAVLIGPL
TYLYLGKEVTRQNEQEFSRLDLLPQLLPVYRDILVRLASLGVEWVQIDEPVLALDLDEDWLRGLDQAYAGLREPADGQAH
GPSALARLPKILLATYFDGVNNHAERLKKLPVEGLHIDLCRAPGQLGIFLEGYPAGRVLSLGIIDGRNIWRADLAPTLAV
LSHAQALLGERLWIAPSCSLLHCPVDLELESSLEPEIRNWLAFSVQKLKEVSILGQGLNQGEHSIRQQLDASTAARQARR
ESSRIHNPVVQDRLNRLTEQDGRRKSPFKVRQKLQRERLQLPLLPTTTIGSFPQTAEIRKARAAFRKGELGNLQYLEAMR
DEIRVAIRKQEEIGLDVLVHGEAERNDMVEYFGEQLWGYVFTENGWVQSYGSRCVKPPILYGDIYRPEPMTADWIRYAQS
QTGKPVKGMLTGPVTMLMWSFVRDDQPRSVTALQLALAIRDEVVDLEEAGIAIIQIDEPAFREGLPLKKRDWNAYLEWAV
HAFRVASSGVRDDSQIHTHMCYSEFNDILPAIADMDADVITIETSRSRMELLDGFGHFKYPNEIGPGVYDIHSPRIPRSR
EMLELLEKACGVIDPDQLWVNPDCGLKTRGWGEVMTALKQMVEAAHLLRARLGYREDESQLEAPESDKEPARVSAG

Sequences:

>Translated_796_residues
MALTHNLGFPRIGAQRELKRALEAYWRGDIGDEQLKTTAAELRKRHWLLQREQGIDLIPAGDFALYDQMLNMTALLSAIP
ARFGADKGKTELDLYFAMARGTVDQPAMEMTKWFDTNYHYIVPEFDAHTCFRIGSEQLFREVAEVQALGIKPKAVLIGPL
TYLYLGKEVTRQNEQEFSRLDLLPQLLPVYRDILVRLASLGVEWVQIDEPVLALDLDEDWLRGLDQAYAGLREPADGQAH
GPSALARLPKILLATYFDGVNNHAERLKKLPVEGLHIDLCRAPGQLGIFLEGYPAGRVLSLGIIDGRNIWRADLAPTLAV
LSHAQALLGERLWIAPSCSLLHCPVDLELESSLEPEIRNWLAFSVQKLKEVSILGQGLNQGEHSIRQQLDASTAARQARR
ESSRIHNPVVQDRLNRLTEQDGRRKSPFKVRQKLQRERLQLPLLPTTTIGSFPQTAEIRKARAAFRKGELGNLQYLEAMR
DEIRVAIRKQEEIGLDVLVHGEAERNDMVEYFGEQLWGYVFTENGWVQSYGSRCVKPPILYGDIYRPEPMTADWIRYAQS
QTGKPVKGMLTGPVTMLMWSFVRDDQPRSVTALQLALAIRDEVVDLEEAGIAIIQIDEPAFREGLPLKKRDWNAYLEWAV
HAFRVASSGVRDDSQIHTHMCYSEFNDILPAIADMDADVITIETSRSRMELLDGFGHFKYPNEIGPGVYDIHSPRIPRSR
EMLELLEKACGVIDPDQLWVNPDCGLKTRGWGEVMTALKQMVEAAHLLRARLGYREDESQLEAPESDKEPARVSAG
>Mature_795_residues
ALTHNLGFPRIGAQRELKRALEAYWRGDIGDEQLKTTAAELRKRHWLLQREQGIDLIPAGDFALYDQMLNMTALLSAIPA
RFGADKGKTELDLYFAMARGTVDQPAMEMTKWFDTNYHYIVPEFDAHTCFRIGSEQLFREVAEVQALGIKPKAVLIGPLT
YLYLGKEVTRQNEQEFSRLDLLPQLLPVYRDILVRLASLGVEWVQIDEPVLALDLDEDWLRGLDQAYAGLREPADGQAHG
PSALARLPKILLATYFDGVNNHAERLKKLPVEGLHIDLCRAPGQLGIFLEGYPAGRVLSLGIIDGRNIWRADLAPTLAVL
SHAQALLGERLWIAPSCSLLHCPVDLELESSLEPEIRNWLAFSVQKLKEVSILGQGLNQGEHSIRQQLDASTAARQARRE
SSRIHNPVVQDRLNRLTEQDGRRKSPFKVRQKLQRERLQLPLLPTTTIGSFPQTAEIRKARAAFRKGELGNLQYLEAMRD
EIRVAIRKQEEIGLDVLVHGEAERNDMVEYFGEQLWGYVFTENGWVQSYGSRCVKPPILYGDIYRPEPMTADWIRYAQSQ
TGKPVKGMLTGPVTMLMWSFVRDDQPRSVTALQLALAIRDEVVDLEEAGIAIIQIDEPAFREGLPLKKRDWNAYLEWAVH
AFRVASSGVRDDSQIHTHMCYSEFNDILPAIADMDADVITIETSRSRMELLDGFGHFKYPNEIGPGVYDIHSPRIPRSRE
MLELLEKACGVIDPDQLWVNPDCGLKTRGWGEVMTALKQMVEAAHLLRARLGYREDESQLEAPESDKEPARVSAG

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]

Homologues:

Organism=Escherichia coli, GI2367304, Length=772, Percent_Identity=53.7564766839378, Blast_Score=821, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=783, Percent_Identity=46.7432950191571, Blast_Score=679, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629 [H]

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]

EC number: =2.1.1.14 [H]

Molecular weight: Translated: 89999; Mature: 89868

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALTHNLGFPRIGAQRELKRALEAYWRGDIGDEQLKTTAAELRKRHWLLQREQGIDLIPA
CCEECCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECC
GDFALYDQMLNMTALLSAIPARFGADKGKTELDLYFAMARGTVDQPAMEMTKWFDTNYHY
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEECCCCCCCHHHHHHHHHCCCCEE
IVPEFDAHTCFRIGSEQLFREVAEVQALGIKPKAVLIGPLTYLYLGKEVTRQNEQEFSRL
EECCCCCHHHHHCCHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHCHHHHHCCHHHHHHH
DLLPQLLPVYRDILVRLASLGVEWVQIDEPVLALDLDEDWLRGLDQAYAGLREPADGQAH
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCC
GPSALARLPKILLATYFDGVNNHAERLKKLPVEGLHIDLCRAPGQLGIFLEGYPAGRVLS
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCEEE
LGIIDGRNIWRADLAPTLAVLSHAQALLGERLWIAPSCSLLHCPVDLELESSLEPEIRNW
EEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEECCCCCCCCCCCCHHHHHH
LAFSVQKLKEVSILGQGLNQGEHSIRQQLDASTAARQARRESSRIHNPVVQDRLNRLTEQ
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
DGRRKSPFKVRQKLQRERLQLPLLPTTTIGSFPQTAEIRKARAAFRKGELGNLQYLEAMR
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHH
DEIRVAIRKQEEIGLDVLVHGEAERNDMVEYFGEQLWGYVFTENGWVQSYGSRCVKPPIL
HHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCEEEEECCCHHHHHCCCCCCCCEE
YGDIYRPEPMTADWIRYAQSQTGKPVKGMLTGPVTMLMWSFVRDDQPRSVTALQLALAIR
ECCCCCCCCCCHHHHHHHHHCCCCCCCCHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
DEVVDLEEAGIAIIQIDEPAFREGLPLKKRDWNAYLEWAVHAFRVASSGVRDDSQIHTHM
HHHHHHHHCCEEEEEECCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
CYSEFNDILPAIADMDADVITIETSRSRMELLDGFGHFKYPNEIGPGVYDIHSPRIPRSR
HHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCHH
EMLELLEKACGVIDPDQLWVNPDCGLKTRGWGEVMTALKQMVEAAHLLRARLGYREDESQ
HHHHHHHHHCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
LEAPESDKEPARVSAG
CCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ALTHNLGFPRIGAQRELKRALEAYWRGDIGDEQLKTTAAELRKRHWLLQREQGIDLIPA
CEECCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECC
GDFALYDQMLNMTALLSAIPARFGADKGKTELDLYFAMARGTVDQPAMEMTKWFDTNYHY
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEECCCCCCCHHHHHHHHHCCCCEE
IVPEFDAHTCFRIGSEQLFREVAEVQALGIKPKAVLIGPLTYLYLGKEVTRQNEQEFSRL
EECCCCCHHHHHCCHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHCHHHHHCCHHHHHHH
DLLPQLLPVYRDILVRLASLGVEWVQIDEPVLALDLDEDWLRGLDQAYAGLREPADGQAH
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCC
GPSALARLPKILLATYFDGVNNHAERLKKLPVEGLHIDLCRAPGQLGIFLEGYPAGRVLS
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCEEE
LGIIDGRNIWRADLAPTLAVLSHAQALLGERLWIAPSCSLLHCPVDLELESSLEPEIRNW
EEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEECCCCCCCCCCCCHHHHHH
LAFSVQKLKEVSILGQGLNQGEHSIRQQLDASTAARQARRESSRIHNPVVQDRLNRLTEQ
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
DGRRKSPFKVRQKLQRERLQLPLLPTTTIGSFPQTAEIRKARAAFRKGELGNLQYLEAMR
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHH
DEIRVAIRKQEEIGLDVLVHGEAERNDMVEYFGEQLWGYVFTENGWVQSYGSRCVKPPIL
HHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCEEEEECCCHHHHHCCCCCCCCEE
YGDIYRPEPMTADWIRYAQSQTGKPVKGMLTGPVTMLMWSFVRDDQPRSVTALQLALAIR
ECCCCCCCCCCHHHHHHHHHCCCCCCCCHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
DEVVDLEEAGIAIIQIDEPAFREGLPLKKRDWNAYLEWAVHAFRVASSGVRDDSQIHTHM
HHHHHHHHCCEEEEEECCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
CYSEFNDILPAIADMDADVITIETSRSRMELLDGFGHFKYPNEIGPGVYDIHSPRIPRSR
HHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCHH
EMLELLEKACGVIDPDQLWVNPDCGLKTRGWGEVMTALKQMVEAAHLLRARLGYREDESQ
HHHHHHHHHCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
LEAPESDKEPARVSAG
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]