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The map label for this gene is munIM [H]

Identifier: 82703721

GI number: 82703721

Start: 2994041

End: 2994721

Strand: Reverse

Name: munIM [H]

Synonym: Nmul_A2608

Alternate gene names: 82703721

Gene position: 2994721-2994041 (Counterclockwise)

Preceding gene: 82703722

Following gene: 82703720

Centisome position: 94.05

GC content: 52.86

Gene sequence:

>681_bases
ATGAATCAAAAAACACCTAACCCAGCCGCAGAGTTGCTGGAACATCTTGCTGACAAGCGTTTCAGCACGATCCTCGCCGA
TCCCCCCTGGCAGTTCCAGAACCGCACCGGCAAGATGGCGCCGGAGCACAAACGGCTGAATCGTTACGACACTATGGAGC
TTGATGAAATCCTGAGCTTACCCGTGCGTGCGCTCGCCGAAGATACCGCACATCTTTACCTGTGGGTGCCGAATGCTCTT
CTACCGGAAGGGCTAAAGGTCATGGAAGCCTGGGGTTTCAAATACAAGAGCAATCTAGTCTGGCACAAGATCCGCAAGGA
TGGCGGACCAGACGGCCGCGGCGTTGGTTTCTACTTTCGCAATGTTACCGAACTCGTGCTATTCGGAGTGCGGGGTAAGA
GTGCTCGTACTTTGGCGCCGGGACGTCGCCAGGTTAATTTTCTGGCTACCCAGAAACGTGAACATTCGCGCAAGCCAGAC
GAGATGTATGAAATTATCGAGTCCTGTAGTCCTGGTCCTTACCTGGAATTGTTTGCTCGTGGCACACGACCGAATTGGCT
GGCGTGGGGTAATGAAGCGGATAACGACTACTACCCGAAATGGCGAACTTACGGAAATCATTCACAAGCCGAATTGATTT
TCTTTGAGCAAGCCAGGCAGCGATACCTGAACCGGAAGTGA

Upstream 100 bases:

>100_bases
ATGGGCAAGCTGTTGTACCGCCTGAATGCTGGCAGACATGGAGGCTGTCCAGTATTAGCGCTAGGTATTACACCAAATCT
CATTACGGATTGGGCAGAAT

Downstream 100 bases:

>100_bases
TTTTCGCTCGCTAGTTGAATCTGAGCAGAATTATCGGCTTTTTACCGAGCCGAATGCTCCGCCTGGAGTTCTGCTGCCAG
AGTTGACTAATCCAGAATAA

Product: MT-A70

Products: NA

Alternate protein names: Adenine-specific methyltransferase MunI; M.MunI [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MNQKTPNPAAELLEHLADKRFSTILADPPWQFQNRTGKMAPEHKRLNRYDTMELDEILSLPVRALAEDTAHLYLWVPNAL
LPEGLKVMEAWGFKYKSNLVWHKIRKDGGPDGRGVGFYFRNVTELVLFGVRGKSARTLAPGRRQVNFLATQKREHSRKPD
EMYEIIESCSPGPYLELFARGTRPNWLAWGNEADNDYYPKWRTYGNHSQAELIFFEQARQRYLNRK

Sequences:

>Translated_226_residues
MNQKTPNPAAELLEHLADKRFSTILADPPWQFQNRTGKMAPEHKRLNRYDTMELDEILSLPVRALAEDTAHLYLWVPNAL
LPEGLKVMEAWGFKYKSNLVWHKIRKDGGPDGRGVGFYFRNVTELVLFGVRGKSARTLAPGRRQVNFLATQKREHSRKPD
EMYEIIESCSPGPYLELFARGTRPNWLAWGNEADNDYYPKWRTYGNHSQAELIFFEQARQRYLNRK
>Mature_226_residues
MNQKTPNPAAELLEHLADKRFSTILADPPWQFQNRTGKMAPEHKRLNRYDTMELDEILSLPVRALAEDTAHLYLWVPNAL
LPEGLKVMEAWGFKYKSNLVWHKIRKDGGPDGRGVGFYFRNVTELVLFGVRGKSARTLAPGRRQVNFLATQKREHSRKPD
EMYEIIESCSPGPYLELFARGTRPNWLAWGNEADNDYYPKWRTYGNHSQAELIFFEQARQRYLNRK

Specific function: This methylase recognizes the double-stranded sequence CAATTG, causes specific methylation on A-3 on both strands, and protects the DNA from cleavage by the MunI endonuclease [H]

COG id: COG4725

COG function: function code TK; Transcriptional activator, adenine-specific DNA methyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MT-A70-like family [H]

Homologues:

Organism=Homo sapiens, GI21361827, Length=182, Percent_Identity=30.2197802197802, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6321246, Length=178, Percent_Identity=29.7752808988764, Blast_Score=71, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21355141, Length=179, Percent_Identity=32.4022346368715, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR007757 [H]

Pfam domain/function: PF05063 MT-A70 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 26366; Mature: 26366

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: PS00092 N6_MTASE ; PS51143 MT_A70

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQKTPNPAAELLEHLADKRFSTILADPPWQFQNRTGKMAPEHKRLNRYDTMELDEILSL
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCHHHHCCCCCCCCHHHHHHH
PVRALAEDTAHLYLWVPNALLPEGLKVMEAWGFKYKSNLVWHKIRKDGGPDGRGVGFYFR
HHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCHHHCCHHHHHHHHCCCCCCCCHHHHHH
NVTELVLFGVRGKSARTLAPGRRQVNFLATQKREHSRKPDEMYEIIESCSPGPYLELFAR
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHEEEC
GTRPNWLAWGNEADNDYYPKWRTYGNHSQAELIFFEQARQRYLNRK
CCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNQKTPNPAAELLEHLADKRFSTILADPPWQFQNRTGKMAPEHKRLNRYDTMELDEILSL
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCHHHHCCCCCCCCHHHHHHH
PVRALAEDTAHLYLWVPNALLPEGLKVMEAWGFKYKSNLVWHKIRKDGGPDGRGVGFYFR
HHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCHHHCCHHHHHHHHCCCCCCCCHHHHHH
NVTELVLFGVRGKSARTLAPGRRQVNFLATQKREHSRKPDEMYEIIESCSPGPYLELFAR
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHEEEC
GTRPNWLAWGNEADNDYYPKWRTYGNHSQAELIFFEQARQRYLNRK
CCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8181741 [H]