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The map label for this gene is lexA2 [H]

Identifier: 82703709

GI number: 82703709

Start: 2981274

End: 2981906

Strand: Direct

Name: lexA2 [H]

Synonym: Nmul_A2596

Alternate gene names: 82703709

Gene position: 2981274-2981906 (Clockwise)

Preceding gene: 82703708

Following gene: 82703710

Centisome position: 93.63

GC content: 52.92

Gene sequence:

>633_bases
ATGCAACCGCTCGACATTTCCTCTTACCTTGGCAGGCTTCAGGATTACTATGCCAGGGAAAAAATCATTCCCTCCACCAC
ACAGTTATCCACGCTATGGAATGTGAAAGCGCGCTCCTGGACGCATCAGATCGTTCAGCGTTTAAAAGAAGAAAATTTTC
TTGAAAATGCACCCGGCAGCCGTCTTCGCCCCGGATTCCGCTTTTTCGAACGGACGGTGGGGCCCGGGGTTCGGGCAGGA
AGACCCCAGCAGACTGCCGATGTTCAGCCTGAACTGTTGCGCATAGACGATTACCTGATCGAAAAACCTTCTCAGACCAT
TCTGTTTCCGGTCAAAGGCGATTCCATGATTGATCTGGGAATACTTGAGGGCGACATCGTGATCATTGAACGCAGCAGTT
CGGCGTCGCCTGGCGAAGTGGTACTGGCGATTGTGGATAGCGAATTCACGCTCAAGGTCCTCGCCAGGGATAAGGAGGGC
TACTACCTGGAAGCACGCAATGAAGCACGAGGCAAGGAATATCCGCCTATCCGGCCTCAGCAGAAACTGGAAATCTACGG
ATTTTATGTAGGCCTGTTCCGGAAAATCCAGGTTCCAGGACCCCGCCTCCATCGCTCTGGTTTGGACCGCTGA

Upstream 100 bases:

>100_bases
CGAACGCTACCGCTGGAAAGCGTACGAGTATGAATCGGCTTGAATAAAGAACATTTGTTCTCTATAATAAGAGCAACTGT
TCTCAGTTTTACCCTTGGTC

Downstream 100 bases:

>100_bases
ATCAATGGGGCGCACCTTTCCATGCCCGTTTCTCCATCTCCAGGGTCCCCGGTGCCCTTATCGCTTTCGCAAATCGAAGC
CCGCTTCAGGAACAGGATAT

Product: putative prophage repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MQPLDISSYLGRLQDYYAREKIIPSTTQLSTLWNVKARSWTHQIVQRLKEENFLENAPGSRLRPGFRFFERTVGPGVRAG
RPQQTADVQPELLRIDDYLIEKPSQTILFPVKGDSMIDLGILEGDIVIIERSSSASPGEVVLAIVDSEFTLKVLARDKEG
YYLEARNEARGKEYPPIRPQQKLEIYGFYVGLFRKIQVPGPRLHRSGLDR

Sequences:

>Translated_210_residues
MQPLDISSYLGRLQDYYAREKIIPSTTQLSTLWNVKARSWTHQIVQRLKEENFLENAPGSRLRPGFRFFERTVGPGVRAG
RPQQTADVQPELLRIDDYLIEKPSQTILFPVKGDSMIDLGILEGDIVIIERSSSASPGEVVLAIVDSEFTLKVLARDKEG
YYLEARNEARGKEYPPIRPQQKLEIYGFYVGLFRKIQVPGPRLHRSGLDR
>Mature_210_residues
MQPLDISSYLGRLQDYYAREKIIPSTTQLSTLWNVKARSWTHQIVQRLKEENFLENAPGSRLRPGFRFFERTVGPGVRAG
RPQQTADVQPELLRIDDYLIEKPSQTILFPVKGDSMIDLGILEGDIVIIERSSSASPGEVVLAIVDSEFTLKVLARDKEG
YYLEARNEARGKEYPPIRPQQKLEIYGFYVGLFRKIQVPGPRLHRSGLDR

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family [H]

Homologues:

Organism=Escherichia coli, GI1787431, Length=123, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24 [H]

EC number: =3.4.21.88 [H]

Molecular weight: Translated: 24002; Mature: 24002

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPLDISSYLGRLQDYYAREKIIPSTTQLSTLWNVKARSWTHQIVQRLKEENFLENAPGS
CCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCC
RLRPGFRFFERTVGPGVRAGRPQQTADVQPELLRIDDYLIEKPSQTILFPVKGDSMIDLG
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHEEHHHHHHCCCCCEEEEEECCCCEEEEE
ILEGDIVIIERSSSASPGEVVLAIVDSEFTLKVLARDKEGYYLEARNEARGKEYPPIRPQ
EECCCEEEEECCCCCCCCEEEEEEECCCEEEEEEEECCCCEEEEECCCCCCCCCCCCCCC
QKLEIYGFYVGLFRKIQVPGPRLHRSGLDR
CCEEEEHEEEHHHHEECCCCCHHHHCCCCC
>Mature Secondary Structure
MQPLDISSYLGRLQDYYAREKIIPSTTQLSTLWNVKARSWTHQIVQRLKEENFLENAPGS
CCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCC
RLRPGFRFFERTVGPGVRAGRPQQTADVQPELLRIDDYLIEKPSQTILFPVKGDSMIDLG
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHEEHHHHHHCCCCCEEEEEECCCCEEEEE
ILEGDIVIIERSSSASPGEVVLAIVDSEFTLKVLARDKEGYYLEARNEARGKEYPPIRPQ
EECCCEEEEECCCCCCCCEEEEEEECCCEEEEEEEECCCCEEEEECCCCCCCCCCCCCCC
QKLEIYGFYVGLFRKIQVPGPRLHRSGLDR
CCEEEEHEEEHHHHEECCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]