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The map label for this gene is pnp

Identifier: 82703668

GI number: 82703668

Start: 2921307

End: 2923412

Strand: Reverse

Name: pnp

Synonym: Nmul_A2553

Alternate gene names: 82703668

Gene position: 2923412-2921307 (Counterclockwise)

Preceding gene: 82703669

Following gene: 82703666

Centisome position: 91.81

GC content: 56.7

Gene sequence:

>2106_bases
TTGAAAATCAAGAAAAGCGTTACGTACGGGTCTCATCAACTAACATTCGAAACCGGAGAAATAGCAAGACAAGCCCATGC
CGCCATTATGGTCAGCATGGGCGATACCGTCGTCCTGGTTACCGTGGTAGGTGCAAAAAGCGCCAAGCAGGGGCAGGATT
TCTTTCCCCTGACGGTGGACTATCAGGAAAGGAGCTACGCGGCTGGCCGGATACCCGGTAGTTTTTTCAAACGTGAAGGC
CGTCCCTCCGAAAAGGAAATTCTTACCTCCCGTCTGATCGATCGCCCGATCCGTCCCCTTTTTCCTGAAAATTTCTACAA
CGAAGTGCAGGTGGTAGCCACCGTCCTGTCTTCCGATAACGAAGTAGATGCCGATATTCCCGCCATGCTGGGCGCATCTG
CCGCGCTGGTGCTCTCGGGCATTCCGTTCAATGGGCCTATCGGCGCCGCGCGGATTGGCTACATCAATGGCGAATATGTA
TTGAATCCCGATACCAGCGCGCTGAAGCAGACACAGCTGAATCTGGTCGTGGCCGGCACGCGGCAGGCTGTGCTGATGGT
GGAATCCGATGCGATGGAGCTGTCGGAGGATGTCATGCTGGGGGCCATCGTTTATGGTCATCAGCAAATGCAGGTTGCCA
TCGATGCGATCAATGAACTGGCGGATGAGGCGGGTGTGACCGCATGGGATTGGGAGCCGCCTGCAAGAGACCCTGCGCTG
GCCGTTAAAATTGCCGAACTGGCGGAGAATGATTTGCGTGCCGCTTTCCGCCAGAAGCAGAAGCAGGTCAGGTCCGAGAC
CATAGACAACATCTGGACAAGAGTCTTTACCGAACTCGGCGTCGAGGACGGGGAGGGTCCTGACGCCCAGGCGGTGAAGG
AGGCCTGTTTTGCGCTGGAATCGAGAATCGTGCGCTCGCAGATTCTGGACGGCGAACCGCGCATCGATGGACGCGATACC
CGCACCGTCCGGCCAATAACGATCCGGACCGGGCTCCTGCCGCGGACCCACGGTTCAGCATTATTTACCCGCGGGGAAAC
TCAGGCGCTGGTAGTTGCAACGCTGGGAACCGGTCGCGATGAGCAGAAAATCGATGCTCTCCAGGGAGATTACTCCGAGC
GTTTCATGCTTCACTACAACATGCCTCCCTATGCGACGGGAGAGACGGGACGAGTTGGAACCCCCAAGCGTCGTGAAATC
GGCCACGGACGGCTGGCAAAGCGAGCCCTGGTTGCAGTTCTGCCTTCTCCGGAAGAGTTTGGTTATTCGCTTCGCGTGGT
TTCTGAAATTACCGAGTCCAATGGCTCCAGTTCCATGGCGTCCGTTTGCGGCGGCTGCCTGGCATTGATGGATGCCGGTG
TACCCCTGAAGGGGCACGTGGCGGGTATTGCCATGGGGCTCATCAAGGAAGGCAACCGGTTTGCCGTGCTCACCGATATT
CTGGGCGACGAAGATCACCTGGGCGACATGGATTTCAAGGTGGCGGGTACCGAAAATGGCATTACCGCCCTGCAGATGGA
TATCAAGATTCAAGGGATCACCAAGGAGATCCTGCATGCTGCCCTGGTACAGGCAAGGGAAGGACGTATGCATATTCTCG
CCATCATGAGGCAGGTGTTGCCGGCCCGGCGCGAGGATATCTCGGAACATGCCCCCCGTATCATCAAGATCAGAATCAAT
CCCGAGAAAATACGTGACGTCATAGGGAAAGGGGGCGCAGTGATCCGTGCGCTTACCGAAGAGACAGGCACCACCATTGA
TATTACGGACGATGGCACTGTCATGATTGCATGTGTCAACGCGGAGGGCGGTGAGCTGGCGAAAAAACGTATCGAGGACA
TCACGGCTGAAGTTGAAGTGGGACGGGTATATGACGGAACGGTACTGAAGCTTCTGGATTTTGGCGCCATCGTCAGCGTT
CTGCCCGGCAAGGACGGCCTGTTGCATATCTCGCAAATCGCCAATGAGCGCGTCAACAATGTCGGCGATCACCTCAAGGA
AGGGCAGGTAGTTCGGGTGAAGGTCCTGGAAGCTGATGACAAGGGAAGGTTGCGTCTCAGCATGAAAGCGGTTGCCACCG
ACGTGGCGGACAACGCCGCCACCTGA

Upstream 100 bases:

>100_bases
TCCTTGAACATCGGAATGGCTTCCGCTTGTATCGGTTGCGCGACCGCGGCTATGGTGCTTGGAGCGCAACCGGAATGATT
TAATCAAAAAGGGCAGTTAA

Downstream 100 bases:

>100_bases
TGGCGGAACAGAGAGAAGTATTTTCCACAAAGAGCAGTGCCGCCATTTTTTTCTGTGCGGTTCCTCGCCCTCTTATCCCG
GCCTTTTCTCCTGTATCTTC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 701; Mature: 701

Protein sequence:

>701_residues
MKIKKSVTYGSHQLTFETGEIARQAHAAIMVSMGDTVVLVTVVGAKSAKQGQDFFPLTVDYQERSYAAGRIPGSFFKREG
RPSEKEILTSRLIDRPIRPLFPENFYNEVQVVATVLSSDNEVDADIPAMLGASAALVLSGIPFNGPIGAARIGYINGEYV
LNPDTSALKQTQLNLVVAGTRQAVLMVESDAMELSEDVMLGAIVYGHQQMQVAIDAINELADEAGVTAWDWEPPARDPAL
AVKIAELAENDLRAAFRQKQKQVRSETIDNIWTRVFTELGVEDGEGPDAQAVKEACFALESRIVRSQILDGEPRIDGRDT
RTVRPITIRTGLLPRTHGSALFTRGETQALVVATLGTGRDEQKIDALQGDYSERFMLHYNMPPYATGETGRVGTPKRREI
GHGRLAKRALVAVLPSPEEFGYSLRVVSEITESNGSSSMASVCGGCLALMDAGVPLKGHVAGIAMGLIKEGNRFAVLTDI
LGDEDHLGDMDFKVAGTENGITALQMDIKIQGITKEILHAALVQAREGRMHILAIMRQVLPARREDISEHAPRIIKIRIN
PEKIRDVIGKGGAVIRALTEETGTTIDITDDGTVMIACVNAEGGELAKKRIEDITAEVEVGRVYDGTVLKLLDFGAIVSV
LPGKDGLLHISQIANERVNNVGDHLKEGQVVRVKVLEADDKGRLRLSMKAVATDVADNAAT

Sequences:

>Translated_701_residues
MKIKKSVTYGSHQLTFETGEIARQAHAAIMVSMGDTVVLVTVVGAKSAKQGQDFFPLTVDYQERSYAAGRIPGSFFKREG
RPSEKEILTSRLIDRPIRPLFPENFYNEVQVVATVLSSDNEVDADIPAMLGASAALVLSGIPFNGPIGAARIGYINGEYV
LNPDTSALKQTQLNLVVAGTRQAVLMVESDAMELSEDVMLGAIVYGHQQMQVAIDAINELADEAGVTAWDWEPPARDPAL
AVKIAELAENDLRAAFRQKQKQVRSETIDNIWTRVFTELGVEDGEGPDAQAVKEACFALESRIVRSQILDGEPRIDGRDT
RTVRPITIRTGLLPRTHGSALFTRGETQALVVATLGTGRDEQKIDALQGDYSERFMLHYNMPPYATGETGRVGTPKRREI
GHGRLAKRALVAVLPSPEEFGYSLRVVSEITESNGSSSMASVCGGCLALMDAGVPLKGHVAGIAMGLIKEGNRFAVLTDI
LGDEDHLGDMDFKVAGTENGITALQMDIKIQGITKEILHAALVQAREGRMHILAIMRQVLPARREDISEHAPRIIKIRIN
PEKIRDVIGKGGAVIRALTEETGTTIDITDDGTVMIACVNAEGGELAKKRIEDITAEVEVGRVYDGTVLKLLDFGAIVSV
LPGKDGLLHISQIANERVNNVGDHLKEGQVVRVKVLEADDKGRLRLSMKAVATDVADNAAT
>Mature_701_residues
MKIKKSVTYGSHQLTFETGEIARQAHAAIMVSMGDTVVLVTVVGAKSAKQGQDFFPLTVDYQERSYAAGRIPGSFFKREG
RPSEKEILTSRLIDRPIRPLFPENFYNEVQVVATVLSSDNEVDADIPAMLGASAALVLSGIPFNGPIGAARIGYINGEYV
LNPDTSALKQTQLNLVVAGTRQAVLMVESDAMELSEDVMLGAIVYGHQQMQVAIDAINELADEAGVTAWDWEPPARDPAL
AVKIAELAENDLRAAFRQKQKQVRSETIDNIWTRVFTELGVEDGEGPDAQAVKEACFALESRIVRSQILDGEPRIDGRDT
RTVRPITIRTGLLPRTHGSALFTRGETQALVVATLGTGRDEQKIDALQGDYSERFMLHYNMPPYATGETGRVGTPKRREI
GHGRLAKRALVAVLPSPEEFGYSLRVVSEITESNGSSSMASVCGGCLALMDAGVPLKGHVAGIAMGLIKEGNRFAVLTDI
LGDEDHLGDMDFKVAGTENGITALQMDIKIQGITKEILHAALVQAREGRMHILAIMRQVLPARREDISEHAPRIIKIRIN
PEKIRDVIGKGGAVIRALTEETGTTIDITDDGTVMIACVNAEGGELAKKRIEDITAEVEVGRVYDGTVLKLLDFGAIVSV
LPGKDGLLHISQIANERVNNVGDHLKEGQVVRVKVLEADDKGRLRLSMKAVATDVADNAAT

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=714, Percent_Identity=38.7955182072829, Blast_Score=441, Evalue=1e-123,
Organism=Escherichia coli, GI145693187, Length=687, Percent_Identity=64.7743813682678, Blast_Score=894, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=714, Percent_Identity=33.6134453781513, Blast_Score=331, Evalue=8e-91,
Organism=Drosophila melanogaster, GI281362905, Length=714, Percent_Identity=37.3949579831933, Blast_Score=435, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24651641, Length=714, Percent_Identity=37.3949579831933, Blast_Score=435, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24651643, Length=714, Percent_Identity=37.3949579831933, Blast_Score=435, Evalue=1e-122,
Organism=Drosophila melanogaster, GI161079377, Length=662, Percent_Identity=36.8580060422961, Blast_Score=392, Evalue=1e-109,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_NITMU (Q2Y5X9)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_413234.1
- ProteinModelPortal:   Q2Y5X9
- STRING:   Q2Y5X9
- GeneID:   3786279
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A2553
- eggNOG:   COG1185
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- PhylomeDB:   Q2Y5X9
- ProtClustDB:   PRK11824
- BioCyc:   NMUL323848:NMUL_A2553-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 75905; Mature: 75905

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKKSVTYGSHQLTFETGEIARQAHAAIMVSMGDTVVLVTVVGAKSAKQGQDFFPLTVD
CCCCCCCCCCCEEEEEEHHHHHHHHHEEEEEECCCEEEEEEEECCCCCCCCCCCCCEEEE
YQERSYAAGRIPGSFFKREGRPSEKEILTSRLIDRPIRPLFPENFYNEVQVVATVLSSDN
CCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCC
EVDADIPAMLGASAALVLSGIPFNGPIGAARIGYINGEYVLNPDTSALKQTQLNLVVAGT
CCCCCCHHHHCCCCEEEEECCCCCCCCCCEEEEEECCEEEECCCHHHHHHEEEEEEEECC
RQAVLMVESDAMELSEDVMLGAIVYGHQQMQVAIDAINELADEAGVTAWDWEPPARDPAL
CEEEEEEECCHHHHHHHHHEEHHEECCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHH
AVKIAELAENDLRAAFRQKQKQVRSETIDNIWTRVFTELGVEDGEGPDAQAVKEACFALE
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
SRIVRSQILDGEPRIDGRDTRTVRPITIRTGLLPRTHGSALFTRGETQALVVATLGTGRD
HHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCEEEECCCCCEEEEEEECCCCC
EQKIDALQGDYSERFMLHYNMPPYATGETGRVGTPKRREIGHGRLAKRALVAVLPSPEEF
HHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHEEECCCHHHH
GYSLRVVSEITESNGSSSMASVCGGCLALMDAGVPLKGHVAGIAMGLIKEGNRFAVLTDI
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEEEEC
LGDEDHLGDMDFKVAGTENGITALQMDIKIQGITKEILHAALVQAREGRMHILAIMRQVL
CCCCCCCCCCCEEEECCCCCCEEEEEEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHH
PARREDISEHAPRIIKIRINPEKIRDVIGKGGAVIRALTEETGTTIDITDDGTVMIACVN
HHHHHHHHHCCCEEEEEEECHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCEEEEEEEC
AEGGELAKKRIEDITAEVEVGRVYDGTVLKLLDFGAIVSVLPGKDGLLHISQIANERVNN
CCCHHHHHHHHHHHHHEEEECEEECCHHHHHHHHHHHHEECCCCCCCEEHHHHHHHHHHH
VGDHLKEGQVVRVKVLEADDKGRLRLSMKAVATDVADNAAT
HHHHHCCCCEEEEEEEECCCCCCEEEEHHHHHHHHHCCCCC
>Mature Secondary Structure
MKIKKSVTYGSHQLTFETGEIARQAHAAIMVSMGDTVVLVTVVGAKSAKQGQDFFPLTVD
CCCCCCCCCCCEEEEEEHHHHHHHHHEEEEEECCCEEEEEEEECCCCCCCCCCCCCEEEE
YQERSYAAGRIPGSFFKREGRPSEKEILTSRLIDRPIRPLFPENFYNEVQVVATVLSSDN
CCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCC
EVDADIPAMLGASAALVLSGIPFNGPIGAARIGYINGEYVLNPDTSALKQTQLNLVVAGT
CCCCCCHHHHCCCCEEEEECCCCCCCCCCEEEEEECCEEEECCCHHHHHHEEEEEEEECC
RQAVLMVESDAMELSEDVMLGAIVYGHQQMQVAIDAINELADEAGVTAWDWEPPARDPAL
CEEEEEEECCHHHHHHHHHEEHHEECCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHH
AVKIAELAENDLRAAFRQKQKQVRSETIDNIWTRVFTELGVEDGEGPDAQAVKEACFALE
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
SRIVRSQILDGEPRIDGRDTRTVRPITIRTGLLPRTHGSALFTRGETQALVVATLGTGRD
HHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCEEEECCCCCEEEEEEECCCCC
EQKIDALQGDYSERFMLHYNMPPYATGETGRVGTPKRREIGHGRLAKRALVAVLPSPEEF
HHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHEEECCCHHHH
GYSLRVVSEITESNGSSSMASVCGGCLALMDAGVPLKGHVAGIAMGLIKEGNRFAVLTDI
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEEEEC
LGDEDHLGDMDFKVAGTENGITALQMDIKIQGITKEILHAALVQAREGRMHILAIMRQVL
CCCCCCCCCCCEEEECCCCCCEEEEEEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHH
PARREDISEHAPRIIKIRINPEKIRDVIGKGGAVIRALTEETGTTIDITDDGTVMIACVN
HHHHHHHHHCCCEEEEEEECHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCEEEEEEEC
AEGGELAKKRIEDITAEVEVGRVYDGTVLKLLDFGAIVSVLPGKDGLLHISQIANERVNN
CCCHHHHHHHHHHHHHEEEECEEECCHHHHHHHHHHHHEECCCCCCCEEHHHHHHHHHHH
VGDHLKEGQVVRVKVLEADDKGRLRLSMKAVATDVADNAAT
HHHHHCCCCEEEEEEEECCCCCCEEEEHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA