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The map label for this gene is asnB [H]

Identifier: 82703634

GI number: 82703634

Start: 2882092

End: 2884035

Strand: Reverse

Name: asnB [H]

Synonym: Nmul_A2519

Alternate gene names: 82703634

Gene position: 2884035-2882092 (Counterclockwise)

Preceding gene: 82703635

Following gene: 82703633

Centisome position: 90.57

GC content: 53.45

Gene sequence:

>1944_bases
ATGTGCGGAATTGTGGGCCTGTTCGATACTCGCGGCAAAAGATCAATCGACCGGCAATTGCTTCTGGGAATAAATCAGGT
TCAGGTACACCGCGGTCCGGATGAAGGAGAAGTTTACACCGAGCCAGGACTGGGTTTCGGTCATCGTCGTCTCTCGATTA
TGGATGTTTCCAGTGGACAGCAACCGCTTTTCAATGAAGATGGTAGTGTGGTGGTGGTTTTCAATGGCGAGATCTATAAC
TTCGAGACGCTTGCCAAAGAACTTTCCAGCCGGGGTCACACCTTCCGGACACATTGTGATACCGAAGTCATTGTTCATGC
CTGGGAAGAGTGGGGTGAACGCTGTGTAAACCGCTTCCGCGGCATGTTCGCATTCGCCTTGTGGGATCGCAACCAGGAGG
TCCTGTTTCTCGCGCGAGACCGGCTGGGCATCAAGCCACTCTACTACAGCCTGCTGGATGATGGGACTTTCGTTTTTGCA
TCAGAGCTCAAGGGAATCCTTGCCCATCCCGGTTTTATCCGGGATATGGACGTTCATGCCATAGAAGATTATTTTGCTTA
TGGATATGTGCCGGAGCCAAAAACCATCTTCAAGCAGGCATATAAGCTTCCCCCGGCGCATACTCTGAAGCTCAGCCGGG
GACGAACCGTACTTCAACCTCAATCCTACTGGGATGTTCCGTTTACGGCGCATTCTCCCCTGAGCCTGGAGGAAGCCCGG
AAAGAGTTGATTGCCCGACTGCGTGAGTCGGTGAAGGTACATTTGATGACCGAAGTGCCCTTGGGCGCTTTTCTGTCCGG
AGGCGTGGATTCGAGCGCGGTGGTGGCAATGATGGCGGGCCTCATGAACGAACCCGTCAATACCTGCTCCATTTCTTTTG
GGGATCCTGCTTTCAACGAATCTCAATATGCGCAAAAGGTGGCGGACCGCTATCATACGCATCATCAGGTAGGCCAGGTT
GACCAGGACGATTTCGACCTCATAGACCAGTTGGCTTCACTGTACGACGAGCCCTTCGCGGATAGTTCGGCCATGCCTAC
CTACCGGGTTTGTCAACTGGCCAGGAAACGGGTCACTGTTGTCTTATCAGGGGATGGGGGGGACGAAAACCTTGGGGGCT
ATCGTCGTTACCGATGGCACCTCTATGAAGAGAGAATGCGATCCATTTTGCCGTTGGGACTTCGCAAACCCTTGTTCGGC
CTGCTCGGGAATCTGTATCCCAAGGCGGATTGGGCGCCGAAAGTATTGCGTGCGAAATCTACCCTTGAAGCTCTCGCGAG
AGATTCTGTGGAAGGCTATTTTCACAGTGTTTCGATCATGAAAGACGGCATGAGGCGCCGGTTGTTCAGCGAGTCCTTCA
GGCGTGAGCTTCAGGGGTATGGGGCAGTAGAGATCCTGCGCAGGCATGCGGATAAATGTCCGGTGACAGATCCTCTTTCT
CGTGTTCAATATCTGGATATGAAAACATACCTGGTGGGTGATATTCTTACCAAGGTAGACCGCGCAAGCATGGCGCATTC
CCTGGAAGTACGCGTGCCTCTCCTGGATCACGAGCTGGTCGAATGGATATCGGGGCTTCCCGCATCCATCAAACTGCGCG
GGCATGAAGGCAAATATGTATTCAAGAAAGCCCTGGAACCCTACCTGTCAGACGACATTCTTTATCGCGACAAGATGGGA
TTCTCGGTTCCGCTCGCAAGCTGGTTTCGCGGCCCCCTGCGTCAGCGGATAAATGATGCACTGCTGGGACCCGTTCTGGT
GGATACGGGATTCTTCAATGGCGCATTTCTGAGGGAAATGCTGGACCAACACCAATCGGGGCGGCGCGACTATAGCGCTT
CCCTGTGGTCGTTGTTGATGTTTGAATCGTTTTTGCGCCACGTGCTTAATGCCGAGCAAGTGCACGCTTCCGGGGAAGGG
CTGAGGCTGGTTCATTTTGCGTGA

Upstream 100 bases:

>100_bases
AGGTCGAGGCAAGGTTCAGTATGGAAGCCATGATGTCCGGATATCTTGAAGTCTATGACCGAGTGTTACGTAGGGTATAA
CGGGCGGAAGGAAAAATAAC

Downstream 100 bases:

>100_bases
GCAGATTGTGGCGGAATCGAGATTCGTCGGCAGATCGGGGCGGAGGGCGAAATCCTGGGGCCCAAGCGTTCCAGGTCTTT
TCACCCGCCGGCTCATCCTG

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 647; Mature: 647

Protein sequence:

>647_residues
MCGIVGLFDTRGKRSIDRQLLLGINQVQVHRGPDEGEVYTEPGLGFGHRRLSIMDVSSGQQPLFNEDGSVVVVFNGEIYN
FETLAKELSSRGHTFRTHCDTEVIVHAWEEWGERCVNRFRGMFAFALWDRNQEVLFLARDRLGIKPLYYSLLDDGTFVFA
SELKGILAHPGFIRDMDVHAIEDYFAYGYVPEPKTIFKQAYKLPPAHTLKLSRGRTVLQPQSYWDVPFTAHSPLSLEEAR
KELIARLRESVKVHLMTEVPLGAFLSGGVDSSAVVAMMAGLMNEPVNTCSISFGDPAFNESQYAQKVADRYHTHHQVGQV
DQDDFDLIDQLASLYDEPFADSSAMPTYRVCQLARKRVTVVLSGDGGDENLGGYRRYRWHLYEERMRSILPLGLRKPLFG
LLGNLYPKADWAPKVLRAKSTLEALARDSVEGYFHSVSIMKDGMRRRLFSESFRRELQGYGAVEILRRHADKCPVTDPLS
RVQYLDMKTYLVGDILTKVDRASMAHSLEVRVPLLDHELVEWISGLPASIKLRGHEGKYVFKKALEPYLSDDILYRDKMG
FSVPLASWFRGPLRQRINDALLGPVLVDTGFFNGAFLREMLDQHQSGRRDYSASLWSLLMFESFLRHVLNAEQVHASGEG
LRLVHFA

Sequences:

>Translated_647_residues
MCGIVGLFDTRGKRSIDRQLLLGINQVQVHRGPDEGEVYTEPGLGFGHRRLSIMDVSSGQQPLFNEDGSVVVVFNGEIYN
FETLAKELSSRGHTFRTHCDTEVIVHAWEEWGERCVNRFRGMFAFALWDRNQEVLFLARDRLGIKPLYYSLLDDGTFVFA
SELKGILAHPGFIRDMDVHAIEDYFAYGYVPEPKTIFKQAYKLPPAHTLKLSRGRTVLQPQSYWDVPFTAHSPLSLEEAR
KELIARLRESVKVHLMTEVPLGAFLSGGVDSSAVVAMMAGLMNEPVNTCSISFGDPAFNESQYAQKVADRYHTHHQVGQV
DQDDFDLIDQLASLYDEPFADSSAMPTYRVCQLARKRVTVVLSGDGGDENLGGYRRYRWHLYEERMRSILPLGLRKPLFG
LLGNLYPKADWAPKVLRAKSTLEALARDSVEGYFHSVSIMKDGMRRRLFSESFRRELQGYGAVEILRRHADKCPVTDPLS
RVQYLDMKTYLVGDILTKVDRASMAHSLEVRVPLLDHELVEWISGLPASIKLRGHEGKYVFKKALEPYLSDDILYRDKMG
FSVPLASWFRGPLRQRINDALLGPVLVDTGFFNGAFLREMLDQHQSGRRDYSASLWSLLMFESFLRHVLNAEQVHASGEG
LRLVHFA
>Mature_647_residues
MCGIVGLFDTRGKRSIDRQLLLGINQVQVHRGPDEGEVYTEPGLGFGHRRLSIMDVSSGQQPLFNEDGSVVVVFNGEIYN
FETLAKELSSRGHTFRTHCDTEVIVHAWEEWGERCVNRFRGMFAFALWDRNQEVLFLARDRLGIKPLYYSLLDDGTFVFA
SELKGILAHPGFIRDMDVHAIEDYFAYGYVPEPKTIFKQAYKLPPAHTLKLSRGRTVLQPQSYWDVPFTAHSPLSLEEAR
KELIARLRESVKVHLMTEVPLGAFLSGGVDSSAVVAMMAGLMNEPVNTCSISFGDPAFNESQYAQKVADRYHTHHQVGQV
DQDDFDLIDQLASLYDEPFADSSAMPTYRVCQLARKRVTVVLSGDGGDENLGGYRRYRWHLYEERMRSILPLGLRKPLFG
LLGNLYPKADWAPKVLRAKSTLEALARDSVEGYFHSVSIMKDGMRRRLFSESFRRELQGYGAVEILRRHADKCPVTDPLS
RVQYLDMKTYLVGDILTKVDRASMAHSLEVRVPLLDHELVEWISGLPASIKLRGHEGKYVFKKALEPYLSDDILYRDKMG
FSVPLASWFRGPLRQRINDALLGPVLVDTGFFNGAFLREMLDQHQSGRRDYSASLWSLLMFESFLRHVLNAEQVHASGEG
LRLVHFA

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=394, Percent_Identity=27.9187817258883, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI168229252, Length=394, Percent_Identity=27.9187817258883, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI168229250, Length=394, Percent_Identity=27.9187817258883, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI296010848, Length=365, Percent_Identity=27.9452054794521, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI1786889, Length=416, Percent_Identity=31.4903846153846, Blast_Score=167, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI71993933, Length=389, Percent_Identity=32.3907455012853, Blast_Score=125, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI25147557, Length=389, Percent_Identity=32.133676092545, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI25147560, Length=289, Percent_Identity=32.1799307958478, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17560178, Length=378, Percent_Identity=27.7777777777778, Blast_Score=106, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6321563, Length=417, Percent_Identity=28.0575539568345, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6325403, Length=418, Percent_Identity=27.0334928229665, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI45553209, Length=398, Percent_Identity=30.6532663316583, Blast_Score=147, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 73506; Mature: 73506

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGLFDTRGKRSIDRQLLLGINQVQVHRGPDEGEVYTEPGLGFGHRRLSIMDVSSGQ
CCCEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCCCEEECCCCCCCCCEEEEEECCCCC
QPLFNEDGSVVVVFNGEIYNFETLAKELSSRGHTFRTHCDTEVIVHAWEEWGERCVNRFR
CCCCCCCCCEEEEECCCEECHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHH
GMFAFALWDRNQEVLFLARDRLGIKPLYYSLLDDGTFVFASELKGILAHPGFIRDMDVHA
HHEEEEEECCCCCEEEEECCCCCCCHHHHHHHCCCCEEEHHHHHHHHCCCCCEECCHHHH
IEDYFAYGYVPEPKTIFKQAYKLPPAHTLKLSRGRTVLQPQSYWDVPFTAHSPLSLEEAR
HHHHHHCCCCCCHHHHHHHHHCCCCCCEEEECCCCEEECCCCCCCCCCCCCCCCCHHHHH
KELIARLRESVKVHLMTEVPLGAFLSGGVDSSAVVAMMAGLMNEPVNTCSISFGDPAFNE
HHHHHHHHHCCEEEEEECCCHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCH
SQYAQKVADRYHTHHQVGQVDQDDFDLIDQLASLYDEPFADSSAMPTYRVCQLARKRVTV
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCEEE
VLSGDGGDENLGGYRRYRWHLYEERMRSILPLGLRKPLFGLLGNLYPKADWAPKVLRAKS
EEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHH
TLEALARDSVEGYFHSVSIMKDGMRRRLFSESFRRELQGYGAVEILRRHADKCPVTDPLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHH
RVQYLDMKTYLVGDILTKVDRASMAHSLEVRVPLLDHELVEWISGLPASIKLRGHEGKYV
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECHHHHHHHHHHCCCCCEEEEECCCCHHH
FKKALEPYLSDDILYRDKMGFSVPLASWFRGPLRQRINDALLGPVLVDTGFFNGAFLREM
HHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHCCCCCCHHHHHHH
LDQHQSGRRDYSASLWSLLMFESFLRHVLNAEQVHASGEGLRLVHFA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCEEEEEEC
>Mature Secondary Structure
MCGIVGLFDTRGKRSIDRQLLLGINQVQVHRGPDEGEVYTEPGLGFGHRRLSIMDVSSGQ
CCCEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCCCEEECCCCCCCCCEEEEEECCCCC
QPLFNEDGSVVVVFNGEIYNFETLAKELSSRGHTFRTHCDTEVIVHAWEEWGERCVNRFR
CCCCCCCCCEEEEECCCEECHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHH
GMFAFALWDRNQEVLFLARDRLGIKPLYYSLLDDGTFVFASELKGILAHPGFIRDMDVHA
HHEEEEEECCCCCEEEEECCCCCCCHHHHHHHCCCCEEEHHHHHHHHCCCCCEECCHHHH
IEDYFAYGYVPEPKTIFKQAYKLPPAHTLKLSRGRTVLQPQSYWDVPFTAHSPLSLEEAR
HHHHHHCCCCCCHHHHHHHHHCCCCCCEEEECCCCEEECCCCCCCCCCCCCCCCCHHHHH
KELIARLRESVKVHLMTEVPLGAFLSGGVDSSAVVAMMAGLMNEPVNTCSISFGDPAFNE
HHHHHHHHHCCEEEEEECCCHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCH
SQYAQKVADRYHTHHQVGQVDQDDFDLIDQLASLYDEPFADSSAMPTYRVCQLARKRVTV
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCEEE
VLSGDGGDENLGGYRRYRWHLYEERMRSILPLGLRKPLFGLLGNLYPKADWAPKVLRAKS
EEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHH
TLEALARDSVEGYFHSVSIMKDGMRRRLFSESFRRELQGYGAVEILRRHADKCPVTDPLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHH
RVQYLDMKTYLVGDILTKVDRASMAHSLEVRVPLLDHELVEWISGLPASIKLRGHEGKYV
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECHHHHHHHHHHCCCCCEEEEECCCCHHH
FKKALEPYLSDDILYRDKMGFSVPLASWFRGPLRQRINDALLGPVLVDTGFFNGAFLREM
HHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHCCCCCCHHHHHHH
LDQHQSGRRDYSASLWSLLMFESFLRHVLNAEQVHASGEGLRLVHFA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]