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The map label for this gene is ppnK

Identifier: 82703537

GI number: 82703537

Start: 2759769

End: 2760653

Strand: Reverse

Name: ppnK

Synonym: Nmul_A2422

Alternate gene names: 82703537

Gene position: 2760653-2759769 (Counterclockwise)

Preceding gene: 82703540

Following gene: 82703536

Centisome position: 86.7

GC content: 56.72

Gene sequence:

>885_bases
ATGAGTTCTTCCTTCCAGACAATAGCACTGATAGGCAAGCACAAGAACCCCGGCATCGTAACACCGTTGTCGAGTTTGGC
GCGCTATCTGCAAAGCCGGAACCTGACCGTGCTGCTCGACAACCTCACCGCTGCAAGCATGGATGAGGACAGCTATCCAG
CTGTAGCCATGGAGGAGATTGGCAGCCGCGCCGACCTCGCAATCGTCCTGGGCGGGGACGGTACCATGCTCAATATCGCG
CGGAAGCTTGCGCCCTTCAATGTGCCGCTGGTCGGCATCAATCAGGGACGCCTCGGCTTTCTTACAGACCTGTCCATTGT
TACCATGCAGCAAACGCTTGGTGCCATACTCGAAGGTCGATATATTACGGAACAGCGCATGCTGCTCTACGCGGAGGTGG
CGCGCAGCAATGTCACCACCTTCGGTGGTCTGGCGCTGAACGACGTGGCTGTCAACCGGGGAATCGGCGGCAACATGATC
GAATTTGAAGTGCGCATCAATGATGAATATGTTTGTTTGCTGCGTTCCGACGGCCTGATTGTTGCCACGCCAACGGGCTC
GACGGCGTATGCGTTGTCGGCAGGCGGTCCCATACTCCATCCGAGCCTCGATCTGGTTGCGCTGGTGCCGGTCAGTCCGC
ATACATTGAGTAATCGTCCGATCGTTGTCGGGCCGGATGCGGCGGTGGAAATCCTGATGCAGCGTACAGCGGTTGCACGT
GTTCATTTCGACAGCCATTCTCATTTCGACCTGGAGGAAAACGACAAGGTAATGGTTCGGCGCTCGCCTCATAGGGTAAC
GTTGCTGCATCCTTCCGACCATAGTTACTACCGCATGTTGCGGGAGAAACTGGGTTGGAGCGGTCTCCCCAGAAATCCGA
CCTGA

Upstream 100 bases:

>100_bases
GAACCCTTTGTCACCTTCCTGCCGATATGGTTTAATCTCGGCAGGAGAAGAGCCATTTCGCGGCTCGGAGATAGCAGAAG
CAGCTAAGGTATTCAATAGA

Downstream 100 bases:

>100_bases
ACGCTGTGGAGTTTCTATGTTGAAGTTTCTTGGTATTCGCGATTTTGTCATCGTTGATAGGATAGACCTGGAATTTGCTC
CCGGTTTTACCGTCCTTACC

Product: NAD(+)/NADH kinase family protein

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MSSSFQTIALIGKHKNPGIVTPLSSLARYLQSRNLTVLLDNLTAASMDEDSYPAVAMEEIGSRADLAIVLGGDGTMLNIA
RKLAPFNVPLVGINQGRLGFLTDLSIVTMQQTLGAILEGRYITEQRMLLYAEVARSNVTTFGGLALNDVAVNRGIGGNMI
EFEVRINDEYVCLLRSDGLIVATPTGSTAYALSAGGPILHPSLDLVALVPVSPHTLSNRPIVVGPDAAVEILMQRTAVAR
VHFDSHSHFDLEENDKVMVRRSPHRVTLLHPSDHSYYRMLREKLGWSGLPRNPT

Sequences:

>Translated_294_residues
MSSSFQTIALIGKHKNPGIVTPLSSLARYLQSRNLTVLLDNLTAASMDEDSYPAVAMEEIGSRADLAIVLGGDGTMLNIA
RKLAPFNVPLVGINQGRLGFLTDLSIVTMQQTLGAILEGRYITEQRMLLYAEVARSNVTTFGGLALNDVAVNRGIGGNMI
EFEVRINDEYVCLLRSDGLIVATPTGSTAYALSAGGPILHPSLDLVALVPVSPHTLSNRPIVVGPDAAVEILMQRTAVAR
VHFDSHSHFDLEENDKVMVRRSPHRVTLLHPSDHSYYRMLREKLGWSGLPRNPT
>Mature_293_residues
SSSFQTIALIGKHKNPGIVTPLSSLARYLQSRNLTVLLDNLTAASMDEDSYPAVAMEEIGSRADLAIVLGGDGTMLNIAR
KLAPFNVPLVGINQGRLGFLTDLSIVTMQQTLGAILEGRYITEQRMLLYAEVARSNVTTFGGLALNDVAVNRGIGGNMIE
FEVRINDEYVCLLRSDGLIVATPTGSTAYALSAGGPILHPSLDLVALVPVSPHTLSNRPIVVGPDAAVEILMQRTAVARV
HFDSHSHFDLEENDKVMVRRSPHRVTLLHPSDHSYYRMLREKLGWSGLPRNPT

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Homo sapiens, GI55743112, Length=328, Percent_Identity=25.609756097561, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI1788968, Length=288, Percent_Identity=42.0138888888889, Blast_Score=240, Evalue=8e-65,
Organism=Saccharomyces cerevisiae, GI6320794, Length=244, Percent_Identity=34.8360655737705, Blast_Score=135, Evalue=8e-33,
Organism=Saccharomyces cerevisiae, GI6322509, Length=241, Percent_Identity=29.8755186721992, Blast_Score=116, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6325068, Length=211, Percent_Identity=28.9099526066351, Blast_Score=99, Evalue=8e-22,
Organism=Drosophila melanogaster, GI28573828, Length=318, Percent_Identity=27.3584905660377, Blast_Score=94, Evalue=8e-20,
Organism=Drosophila melanogaster, GI28573826, Length=317, Percent_Identity=27.1293375394322, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI28573830, Length=316, Percent_Identity=26.8987341772152, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI28573832, Length=317, Percent_Identity=27.1293375394322, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI161077047, Length=317, Percent_Identity=27.1293375394322, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI20129957, Length=277, Percent_Identity=28.5198555956679, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281363321, Length=277, Percent_Identity=28.5198555956679, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24653422, Length=277, Percent_Identity=28.5198555956679, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI281363323, Length=277, Percent_Identity=28.5198555956679, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24653424, Length=277, Percent_Identity=28.5198555956679, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK_NITMU (Q2Y6B0)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_413103.1
- ProteinModelPortal:   Q2Y6B0
- SMR:   Q2Y6B0
- STRING:   Q2Y6B0
- GeneID:   3785514
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A2422
- eggNOG:   COG0061
- HOGENOM:   HBG713904
- OMA:   FESFMID
- PhylomeDB:   Q2Y6B0
- ProtClustDB:   PRK02155
- BioCyc:   NMUL323848:NMUL_A2422-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 31950; Mature: 31819

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSFQTIALIGKHKNPGIVTPLSSLARYLQSRNLTVLLDNLTAASMDEDSYPAVAMEEI
CCCCCEEEEEEECCCCCCEECCHHHHHHHHHCCCEEEEEECCEECCCCCCCCCCHHHHHC
GSRADLAIVLGGDGTMLNIARKLAPFNVPLVGINQGRLGFLTDLSIVTMQQTLGAILEGR
CCCCCEEEEECCCCCEEHHHHHCCCCCCEEEEECCCCEEEEECHHHHHHHHHHHHHHCCC
YITEQRMLLYAEVARSNVTTFGGLALNDVAVNRGIGGNMIEFEVRINDEYVCLLRSDGLI
EECHHHHHHEEEHHHCCCCEECCEEEEEEEEECCCCCCEEEEEEEECCCEEEEEECCCEE
VATPTGSTAYALSAGGPILHPSLDLVALVPVSPHTLSNRPIVVGPDAAVEILMQRTAVAR
EECCCCCCEEEEECCCCEECCCCCEEEEEECCCCCCCCCCEEECCHHHHHHHHHHHHEEE
VHFDSHSHFDLEENDKVMVRRSPHRVTLLHPSDHSYYRMLREKLGWSGLPRNPT
EEECCCCCCCCCCCCEEEEEECCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SSSFQTIALIGKHKNPGIVTPLSSLARYLQSRNLTVLLDNLTAASMDEDSYPAVAMEEI
CCCCEEEEEEECCCCCCEECCHHHHHHHHHCCCEEEEEECCEECCCCCCCCCCHHHHHC
GSRADLAIVLGGDGTMLNIARKLAPFNVPLVGINQGRLGFLTDLSIVTMQQTLGAILEGR
CCCCCEEEEECCCCCEEHHHHHCCCCCCEEEEECCCCEEEEECHHHHHHHHHHHHHHCCC
YITEQRMLLYAEVARSNVTTFGGLALNDVAVNRGIGGNMIEFEVRINDEYVCLLRSDGLI
EECHHHHHHEEEHHHCCCCEECCEEEEEEEEECCCCCCEEEEEEEECCCEEEEEECCCEE
VATPTGSTAYALSAGGPILHPSLDLVALVPVSPHTLSNRPIVVGPDAAVEILMQRTAVAR
EECCCCCCEEEEECCCCEECCCCCEEEEEECCCCCCCCCCEEECCHHHHHHHHHHHHEEE
VHFDSHSHFDLEENDKVMVRRSPHRVTLLHPSDHSYYRMLREKLGWSGLPRNPT
EEECCCCCCCCCCCCEEEEEECCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA