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The map label for this gene is xthA [H]

Identifier: 82703533

GI number: 82703533

Start: 2755156

End: 2755989

Strand: Reverse

Name: xthA [H]

Synonym: Nmul_A2418

Alternate gene names: 82703533

Gene position: 2755989-2755156 (Counterclockwise)

Preceding gene: 82703536

Following gene: 82703529

Centisome position: 86.55

GC content: 54.56

Gene sequence:

>834_bases
ATGAAGATAGCCACCTGGAACGTAAATTCTCTTAAAGTTCGCCTTCCGCAGGTTCTGGAATGGCTGACATTGAATCAGCC
GGACGTGTTGTGTCTCCAGGAGACCAAGCTGCAAGATGAGCATTTCCCCGTAAGCGAGATTGCGGCTGCGGGTTATCAGA
CGATCTATTCCGGCCAGAAAACATACAATGGCGTCGCGCTGCTCAGCAGGCAGCCGGTGAGCGAAGTCTTTACCGGCATG
CCGGGCTTCGAGGATCCGCAAAAACGTCTTCTCGGGGGAGTTCATGACGACGTTTGCGTCGTTTGCATTTATGTTCCCAA
TGGGGAGCATGTTACATCCGAGAAGTATCAGTACAAGTTGAGGTGGCTTGCAGCGTTGCGGGAATGGCTGCGCGAGATGT
TGAGCAGGCACCGGAAGCTGGTGGTGCTGGGAGACTTCAATATCGCCCCGGAAGACTGCGATGTGCACGACAGCAAGTTA
TGGGAAGGAAAAGTCCTGTTCAGCGAGCTGGAGCGCGCGGCCTTTCGTGAAGTGCTCGATCTTGGCTTTATTGACAGCTT
TCGTCTGTTCGATCAGCCTGAGAAATCGTTTACCTGGTGGGATTATCGAATGATGGCTTTTCGCCGCAACATGGGCTTGC
GGATAGACCACATTCTGCTGAGCCGTGAGCTTGCGGGCATGTGCACGGCTTGTGTCATAGATAAGGCTACCCGCAGACTG
GAGAGGCCGTCCGACCATGCGCCCGTGATAGTGGAGCTTGGCGGGAGCGTACAGGAAAAGGAAAGAAGCGCAGGGTCGGA
AGCTTCCATTCGCGTCTCGGCAAACGAGCCATGA

Upstream 100 bases:

>100_bases
GTTGCAATACGTTTTCATGATATCACAAGGCGAATTGATAACTGTTCAGTATTTTGAATAATGGATATACTTCAGCCCAA
GCGGCAGCAGAGCAAAAAAC

Downstream 100 bases:

>100_bases
GAAGCGCCTATTACAGCTCTCCGCGGATCATTCATCTTCGAATGAGAAGGGGAAGGCTGACTGGTAAGGCAGTGTCAGGC
AGCTACGCGCGCTAGCGAAT

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MKIATWNVNSLKVRLPQVLEWLTLNQPDVLCLQETKLQDEHFPVSEIAAAGYQTIYSGQKTYNGVALLSRQPVSEVFTGM
PGFEDPQKRLLGGVHDDVCVVCIYVPNGEHVTSEKYQYKLRWLAALREWLREMLSRHRKLVVLGDFNIAPEDCDVHDSKL
WEGKVLFSELERAAFREVLDLGFIDSFRLFDQPEKSFTWWDYRMMAFRRNMGLRIDHILLSRELAGMCTACVIDKATRRL
ERPSDHAPVIVELGGSVQEKERSAGSEASIRVSANEP

Sequences:

>Translated_277_residues
MKIATWNVNSLKVRLPQVLEWLTLNQPDVLCLQETKLQDEHFPVSEIAAAGYQTIYSGQKTYNGVALLSRQPVSEVFTGM
PGFEDPQKRLLGGVHDDVCVVCIYVPNGEHVTSEKYQYKLRWLAALREWLREMLSRHRKLVVLGDFNIAPEDCDVHDSKL
WEGKVLFSELERAAFREVLDLGFIDSFRLFDQPEKSFTWWDYRMMAFRRNMGLRIDHILLSRELAGMCTACVIDKATRRL
ERPSDHAPVIVELGGSVQEKERSAGSEASIRVSANEP
>Mature_277_residues
MKIATWNVNSLKVRLPQVLEWLTLNQPDVLCLQETKLQDEHFPVSEIAAAGYQTIYSGQKTYNGVALLSRQPVSEVFTGM
PGFEDPQKRLLGGVHDDVCVVCIYVPNGEHVTSEKYQYKLRWLAALREWLREMLSRHRKLVVLGDFNIAPEDCDVHDSKL
WEGKVLFSELERAAFREVLDLGFIDSFRLFDQPEKSFTWWDYRMMAFRRNMGLRIDHILLSRELAGMCTACVIDKATRRL
ERPSDHAPVIVELGGSVQEKERSAGSEASIRVSANEP

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=262, Percent_Identity=33.969465648855, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI18375503, Length=262, Percent_Identity=33.969465648855, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI18375501, Length=262, Percent_Identity=33.969465648855, Blast_Score=129, Evalue=3e-30,
Organism=Escherichia coli, GI1788046, Length=262, Percent_Identity=37.4045801526718, Blast_Score=165, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI71989536, Length=262, Percent_Identity=29.0076335877863, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI221330655, Length=257, Percent_Identity=28.7937743190661, Blast_Score=101, Evalue=4e-22,
Organism=Drosophila melanogaster, GI17136678, Length=260, Percent_Identity=28.8461538461538, Blast_Score=101, Evalue=6e-22,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 31728; Mature: 31728

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIATWNVNSLKVRLPQVLEWLTLNQPDVLCLQETKLQDEHFPVSEIAAAGYQTIYSGQK
CEEEEECCCHHEEEHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCC
TYNGVALLSRQPVSEVFTGMPGFEDPQKRLLGGVHDDVCVVCIYVPNGEHVTSEKYQYKL
CCCCEEEECCCCHHHHHCCCCCCCCHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHH
RWLAALREWLREMLSRHRKLVVLGDFNIAPEDCDVHDSKLWEGKVLFSELERAAFREVLD
HHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHH
LGFIDSFRLFDQPEKSFTWWDYRMMAFRRNMGLRIDHILLSRELAGMCTACVIDKATRRL
HCCCCCHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ERPSDHAPVIVELGGSVQEKERSAGSEASIRVSANEP
CCCCCCCCEEEEECCCHHHHHHCCCCCCEEEEECCCC
>Mature Secondary Structure
MKIATWNVNSLKVRLPQVLEWLTLNQPDVLCLQETKLQDEHFPVSEIAAAGYQTIYSGQK
CEEEEECCCHHEEEHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCC
TYNGVALLSRQPVSEVFTGMPGFEDPQKRLLGGVHDDVCVVCIYVPNGEHVTSEKYQYKL
CCCCEEEECCCCHHHHHCCCCCCCCHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHH
RWLAALREWLREMLSRHRKLVVLGDFNIAPEDCDVHDSKLWEGKVLFSELERAAFREVLD
HHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHH
LGFIDSFRLFDQPEKSFTWWDYRMMAFRRNMGLRIDHILLSRELAGMCTACVIDKATRRL
HCCCCCHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ERPSDHAPVIVELGGSVQEKERSAGSEASIRVSANEP
CCCCCCCCEEEEECCCHHHHHHCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]