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The map label for this gene is gspE [C]

Identifier: 82703471

GI number: 82703471

Start: 2680898

End: 2682256

Strand: Reverse

Name: gspE [C]

Synonym: Nmul_A2354

Alternate gene names: 82703471

Gene position: 2682256-2680898 (Counterclockwise)

Preceding gene: 82703472

Following gene: 82703470

Centisome position: 84.24

GC content: 61.66

Gene sequence:

>1359_bases
ATGTCGATACGAGAACGTCTCCAGAGCGTGAAGGAAACCGGGTTGGCTGAGCATCATCCCGCGCCGGTCACGGCCCTGCA
CGATATCCCCGCCTATGAGGAATTGAAGGCCCGCGTCCACCAGAAGCTGCTCGATCGGGTGGATCTGGCAGTCATGGAAA
GCCTGCCGGCTGAACGCCTGCTGATGGAGATCAGGAACCTGGTGGAGCGGTTGCTGGTCGAGGAATCCGTCCCCATCAAC
GAGGCGGAGCGGCAGGGCATCGTCCGCGACATCCAGAACGAAGTGCTCGGCCTGGGTCCGCTCGAGCCCTTGCTGGCAGA
CCCGACCATTTCCGACATCCTGGTGAATACCCATCGGCAGGTCTATGTCGAGCGGCGGGGACGTCTGGAACTGACCGACA
CCCACTTTGCCAACGAAAAGCACTTGCGAAAGATCATTGACAGGATCGTGTCGCGTGTCGGGCGGCGCGTGGATGAGTCC
AGTCCCATGGTCGATGCGCGTCTGCCCGACGGTTCACGCGTCAATGCCATCATTCCGCCCCTGGCGATCGATGGCTCGCT
GCTTTCGATCCGGCGTTTTTCCGTCAAGCCGCTCAAGATGAACGATCTCATGGCGTACAAGTCGCTGACCCCGGAAATGG
GCGAGATCATCAGCGGACTGGTCAAGGGCAAGTGCAGCATACTCATTTCCGGCGGCACCGGCAGCGGCAAGACCACGCTG
CTCAATATCATGTCCGGTTTCATTCCGTCCTCCGAACGGATCGTGACCATCGAGGACGCGGCCGAGCTGCAGCTGCAGCA
GCCCCACGTGGTGCGGCTGGAGACGCGCCCGCCCAACGTCGAGGGCAAGGGGGAAATCTCGCAACGGGCGCTGGTGAAGA
ACAGCCTGCGCATGCGCCCCGACCGGGTGATCATAGGGGAAGTGCGCGGAGCCGAGGCGCTGGATATGCTGCAGGCCATG
AACACCGGTCACGAAGGTTCCATGGCCACGATCCATGCGAATACGCCGAGGGATGCCCTGGGCAGGGTTGAAAACATGGT
GAACATGGCCGGGTTGAACCTGCCCATCAAGGCGGTCCGCCACCAGATCAGTTCGGCCATCTGGGTGGTGATCCAGGTCT
TGCGCCTGACTGACGGCAAACGCAAGGTGACGAGCATCCAGGAAATCACCGGCATGGAGGGGGACATTATCACGATGCAG
GAAATCTATGCTTTCGAGCAGACGGGTATCGCGGCGGACGGAACCGTGCAGGGCCATTTCCGCGCCACCGGCATCCGCCC
CAAGTTCGCCGAGCGACTGCGTGTGCATGGGATACCGCTGCGCGAGGAGCTGTTCGATCCCTCGCGCCGGTATACATAG

Upstream 100 bases:

>100_bases
CAGGAGATTGCGCATGAACTATGCCGGGGCGCCGGCCAGGGAAACCTGCTCAGGAAATTGTTTGCATTCTAGATAACCCC
GATTTTATGGACTGACAGTC

Downstream 100 bases:

>100_bases
GGGGCGAGGAGACGGGAATGGACATTCTTTACTATCTGTTTCTCATATTCTGCTTCCTTGCGGTCGTGCTGCTGCTGGAA
GGAGGCTACCTGGCCTGGCA

Product: type II secretion system protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MSIRERLQSVKETGLAEHHPAPVTALHDIPAYEELKARVHQKLLDRVDLAVMESLPAERLLMEIRNLVERLLVEESVPIN
EAERQGIVRDIQNEVLGLGPLEPLLADPTISDILVNTHRQVYVERRGRLELTDTHFANEKHLRKIIDRIVSRVGRRVDES
SPMVDARLPDGSRVNAIIPPLAIDGSLLSIRRFSVKPLKMNDLMAYKSLTPEMGEIISGLVKGKCSILISGGTGSGKTTL
LNIMSGFIPSSERIVTIEDAAELQLQQPHVVRLETRPPNVEGKGEISQRALVKNSLRMRPDRVIIGEVRGAEALDMLQAM
NTGHEGSMATIHANTPRDALGRVENMVNMAGLNLPIKAVRHQISSAIWVVIQVLRLTDGKRKVTSIQEITGMEGDIITMQ
EIYAFEQTGIAADGTVQGHFRATGIRPKFAERLRVHGIPLREELFDPSRRYT

Sequences:

>Translated_452_residues
MSIRERLQSVKETGLAEHHPAPVTALHDIPAYEELKARVHQKLLDRVDLAVMESLPAERLLMEIRNLVERLLVEESVPIN
EAERQGIVRDIQNEVLGLGPLEPLLADPTISDILVNTHRQVYVERRGRLELTDTHFANEKHLRKIIDRIVSRVGRRVDES
SPMVDARLPDGSRVNAIIPPLAIDGSLLSIRRFSVKPLKMNDLMAYKSLTPEMGEIISGLVKGKCSILISGGTGSGKTTL
LNIMSGFIPSSERIVTIEDAAELQLQQPHVVRLETRPPNVEGKGEISQRALVKNSLRMRPDRVIIGEVRGAEALDMLQAM
NTGHEGSMATIHANTPRDALGRVENMVNMAGLNLPIKAVRHQISSAIWVVIQVLRLTDGKRKVTSIQEITGMEGDIITMQ
EIYAFEQTGIAADGTVQGHFRATGIRPKFAERLRVHGIPLREELFDPSRRYT
>Mature_451_residues
SIRERLQSVKETGLAEHHPAPVTALHDIPAYEELKARVHQKLLDRVDLAVMESLPAERLLMEIRNLVERLLVEESVPINE
AERQGIVRDIQNEVLGLGPLEPLLADPTISDILVNTHRQVYVERRGRLELTDTHFANEKHLRKIIDRIVSRVGRRVDESS
PMVDARLPDGSRVNAIIPPLAIDGSLLSIRRFSVKPLKMNDLMAYKSLTPEMGEIISGLVKGKCSILISGGTGSGKTTLL
NIMSGFIPSSERIVTIEDAAELQLQQPHVVRLETRPPNVEGKGEISQRALVKNSLRMRPDRVIIGEVRGAEALDMLQAMN
TGHEGSMATIHANTPRDALGRVENMVNMAGLNLPIKAVRHQISSAIWVVIQVLRLTDGKRKVTSIQEITGMEGDIITMQE
IYAFEQTGIAADGTVQGHFRATGIRPKFAERLRVHGIPLREELFDPSRRYT

Specific function: Involved In A General Secretion Pathway (Gsp) For The Export Of Proteins (By Similarity). [C]

COG id: COG4962

COG function: function code U; Flp pilus assembly protein, ATPase CpaF

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022399
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 50310; Mature: 50179

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: PS00227 TUBULIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIRERLQSVKETGLAEHHPAPVTALHDIPAYEELKARVHQKLLDRVDLAVMESLPAERL
CCHHHHHHHHHHCCCCCCCCCCEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LMEIRNLVERLLVEESVPINEAERQGIVRDIQNEVLGLGPLEPLLADPTISDILVNTHRQ
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHH
VYVERRGRLELTDTHFANEKHLRKIIDRIVSRVGRRVDESSPMVDARLPDGSRVNAIIPP
EEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEECCC
LAIDGSLLSIRRFSVKPLKMNDLMAYKSLTPEMGEIISGLVKGKCSILISGGTGSGKTTL
EEECCCEEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHH
LNIMSGFIPSSERIVTIEDAAELQLQQPHVVRLETRPPNVEGKGEISQRALVKNSLRMRP
HHHHHHCCCCCCCEEEEECHHHHEECCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCC
DRVIIGEVRGAEALDMLQAMNTGHEGSMATIHANTPRDALGRVENMVNMAGLNLPIKAVR
CEEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHH
HQISSAIWVVIQVLRLTDGKRKVTSIQEITGMEGDIITMQEIYAFEQTGIAADGTVQGHF
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEHHHHHHHHHCCCCCCCEEECCE
RATGIRPKFAERLRVHGIPLREELFDPSRRYT
EECCCCHHHHHHHHHCCCCHHHHHCCCCCCCC
>Mature Secondary Structure 
SIRERLQSVKETGLAEHHPAPVTALHDIPAYEELKARVHQKLLDRVDLAVMESLPAERL
CHHHHHHHHHHCCCCCCCCCCEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LMEIRNLVERLLVEESVPINEAERQGIVRDIQNEVLGLGPLEPLLADPTISDILVNTHRQ
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHH
VYVERRGRLELTDTHFANEKHLRKIIDRIVSRVGRRVDESSPMVDARLPDGSRVNAIIPP
EEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEECCC
LAIDGSLLSIRRFSVKPLKMNDLMAYKSLTPEMGEIISGLVKGKCSILISGGTGSGKTTL
EEECCCEEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHH
LNIMSGFIPSSERIVTIEDAAELQLQQPHVVRLETRPPNVEGKGEISQRALVKNSLRMRP
HHHHHHCCCCCCCEEEEECHHHHEECCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCC
DRVIIGEVRGAEALDMLQAMNTGHEGSMATIHANTPRDALGRVENMVNMAGLNLPIKAVR
CEEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHH
HQISSAIWVVIQVLRLTDGKRKVTSIQEITGMEGDIITMQEIYAFEQTGIAADGTVQGHF
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEHHHHHHHHHCCCCCCCEEECCE
RATGIRPKFAERLRVHGIPLREELFDPSRRYT
EECCCCHHHHHHHHHCCCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036; 9634230 [H]