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The map label for this gene is groEL

Identifier: 82703461

GI number: 82703461

Start: 2671276

End: 2672934

Strand: Reverse

Name: groEL

Synonym: Nmul_A2344

Alternate gene names: 82703461

Gene position: 2672934-2671276 (Counterclockwise)

Preceding gene: 82703462

Following gene: 82703460

Centisome position: 83.94

GC content: 57.56

Gene sequence:

>1659_bases
ATGGCAGCGAAAGAGGTTAAATTCAGCGATTCAGCTCGCCACAAGATGGTGAATGGAGTCAACATCCTGGCCGATGCGGT
AAAAGTCACTCTCGGACCCAAGGGGCGTAATGTCGTCCTGGAGCGTTCCTACGGCTCGCCCACCATCACCAAGGATGGAG
TTTCAGTAGCGAAGGAAATCGAACTGAAAGACAAGTTCGAGAACATGGGTGCGCAGATGGTAAAGGAAGTCGCCAGCAAG
ACTTCCGATGTTGCCGGTGACGGCACGACCACGGCTACCGTTCTGGCGCAATCCATTGTCAAGGAAGGCATGAAGTACGT
CGCCGCCGGGATGAATCCCATGGATCTCAAGCGGGGAATCGACAAGGCGGTCGTCGCCACGGTTGAGGAACTGAAGAAAC
TTTCCAAACCCTGCACCACCGGCAAGGAAATCGCGCAGGTCGGCAGCATTTCCGCCAATTCCGATCCCGAAATCGGCAAG
ATCATTGCTGATGCAATGGAAAAAGTTGGCAAGGAAGGTGTCATCACCGTGGAGGACGGTTCCGGGCTGCAAAACGAACT
GGAAGTAGTCGAAGGCATGCAGTTCGATCGTGGCTACCTGTCGCCCTACTTCATTAACAATGCGGACAGGCAGATCGCAC
TGCTGGAAAGCCCGTTCATCCTCTTGCACGACAAGAAAATCTCCAACATCCGTGACTTGCTGCCAGTACTGGAACAGGTG
GCGAAGGCCGGCAAGCCGTTGCTGATCATCGCTGAAGATGTCGATGGTGAAGCGCTCGCCACGCTGGTGGTCAACAACAT
CCGCGGCATTCTCAAGACCTGCGCAGTGAAAGCGCCCGGCTTTGGTGACCGCCGCAAAGCCATGCTCGAGGATATCGCCA
TTCTCACCGGCGGTACGGTGATTGCCGAAGAAGTGGGCCTCTCGCTGGAAAAGGCGACCCTGGCGGAACTGGGGCAAGCC
AAGCGGGTGGAAGTCGGCAAGGAGGAAACTACCATCATCGACGGCGCCGGGGATACCCAGAACATCGAAGGCCGTGTGAA
GCAGATCCGCGCCCAGATCGAAGAAGCGACCAGCGACTACGATAAGGAGAAACTGCAGGAGCGCGTGGCGAAGCTGGCCG
GCGGCGTGGCGTTGATCAAAGTGGGCGCTGCGACAGAAGTGGAGATGAAGGAGAAAAAAGCGCGGGTGGAAGATGCGTTG
CATGCTACCCGGGCTGCGGTGGAAGAAGGTATCGTTCCCGGCGGCGGTGTCGCGCTCCTGCGCACCCGTAGCGCGGTGTC
GAACCTGAAAGGCGACAACCATGACCAGGATGCCGGAATCAAGATCGTTCTGCGTGCCCTGGAAGAGCCCTTGCGTCAGA
TCGTTGCCAACTGCGGTGATGAGCCTTCCGTGGTCATCAACAAGGTTCTGGAAGGAACGGAGAACTTCGGCTATAACGCG
GCCAGCAGTGAATATGGTGACATGGTTCAAATGGGTGTGCTCGACCCCACCAAAGTGACGCGTTATGCCTTGCAGCATGC
TGCTTCTATCGCAGGTCTGATGCTCACCACGGATGCGCTGGTAGCGGAAGTGCCCAAGGAAGAGGGTGCCGGCGGCGGTA
TGGGCGGAGGCATGGGCGGTATGGGTGGCATGGGTGGCATGGGCGGCATGGACATGTAA

Upstream 100 bases:

>100_bases
CTTCTGGTGATGCGCGAAGAAGACATCATGGGCGTTGTTGAAGGTTAATTTTCGCAAGACGTCTTGCACAATATTTTCTG
GAAATTAGGAGAAACGGATT

Downstream 100 bases:

>100_bases
GGTTTGCCGACCGCTGCACCAATCGTGCAATAAGCTGTGCAATAAAAAACCCCGCGGCTGCGGGGTTTTTTATTATCGGA
GGCGGTGCGTCAGCCAGCAT

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein; Protein Cpn60

Number of amino acids: Translated: 552; Mature: 551

Protein sequence:

>552_residues
MAAKEVKFSDSARHKMVNGVNILADAVKVTLGPKGRNVVLERSYGSPTITKDGVSVAKEIELKDKFENMGAQMVKEVASK
TSDVAGDGTTTATVLAQSIVKEGMKYVAAGMNPMDLKRGIDKAVVATVEELKKLSKPCTTGKEIAQVGSISANSDPEIGK
IIADAMEKVGKEGVITVEDGSGLQNELEVVEGMQFDRGYLSPYFINNADRQIALLESPFILLHDKKISNIRDLLPVLEQV
AKAGKPLLIIAEDVDGEALATLVVNNIRGILKTCAVKAPGFGDRRKAMLEDIAILTGGTVIAEEVGLSLEKATLAELGQA
KRVEVGKEETTIIDGAGDTQNIEGRVKQIRAQIEEATSDYDKEKLQERVAKLAGGVALIKVGAATEVEMKEKKARVEDAL
HATRAAVEEGIVPGGGVALLRTRSAVSNLKGDNHDQDAGIKIVLRALEEPLRQIVANCGDEPSVVINKVLEGTENFGYNA
ASSEYGDMVQMGVLDPTKVTRYALQHAASIAGLMLTTDALVAEVPKEEGAGGGMGGGMGGMGGMGGMGGMDM

Sequences:

>Translated_552_residues
MAAKEVKFSDSARHKMVNGVNILADAVKVTLGPKGRNVVLERSYGSPTITKDGVSVAKEIELKDKFENMGAQMVKEVASK
TSDVAGDGTTTATVLAQSIVKEGMKYVAAGMNPMDLKRGIDKAVVATVEELKKLSKPCTTGKEIAQVGSISANSDPEIGK
IIADAMEKVGKEGVITVEDGSGLQNELEVVEGMQFDRGYLSPYFINNADRQIALLESPFILLHDKKISNIRDLLPVLEQV
AKAGKPLLIIAEDVDGEALATLVVNNIRGILKTCAVKAPGFGDRRKAMLEDIAILTGGTVIAEEVGLSLEKATLAELGQA
KRVEVGKEETTIIDGAGDTQNIEGRVKQIRAQIEEATSDYDKEKLQERVAKLAGGVALIKVGAATEVEMKEKKARVEDAL
HATRAAVEEGIVPGGGVALLRTRSAVSNLKGDNHDQDAGIKIVLRALEEPLRQIVANCGDEPSVVINKVLEGTENFGYNA
ASSEYGDMVQMGVLDPTKVTRYALQHAASIAGLMLTTDALVAEVPKEEGAGGGMGGGMGGMGGMGGMGGMDM
>Mature_551_residues
AAKEVKFSDSARHKMVNGVNILADAVKVTLGPKGRNVVLERSYGSPTITKDGVSVAKEIELKDKFENMGAQMVKEVASKT
SDVAGDGTTTATVLAQSIVKEGMKYVAAGMNPMDLKRGIDKAVVATVEELKKLSKPCTTGKEIAQVGSISANSDPEIGKI
IADAMEKVGKEGVITVEDGSGLQNELEVVEGMQFDRGYLSPYFINNADRQIALLESPFILLHDKKISNIRDLLPVLEQVA
KAGKPLLIIAEDVDGEALATLVVNNIRGILKTCAVKAPGFGDRRKAMLEDIAILTGGTVIAEEVGLSLEKATLAELGQAK
RVEVGKEETTIIDGAGDTQNIEGRVKQIRAQIEEATSDYDKEKLQERVAKLAGGVALIKVGAATEVEMKEKKARVEDALH
ATRAAVEEGIVPGGGVALLRTRSAVSNLKGDNHDQDAGIKIVLRALEEPLRQIVANCGDEPSVVINKVLEGTENFGYNAA
SSEYGDMVQMGVLDPTKVTRYALQHAASIAGLMLTTDALVAEVPKEEGAGGGMGGGMGGMGGMGGMGGMDM

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family

Homologues:

Organism=Homo sapiens, GI41399285, Length=527, Percent_Identity=53.7001897533207, Blast_Score=550, Evalue=1e-156,
Organism=Homo sapiens, GI31542947, Length=527, Percent_Identity=53.7001897533207, Blast_Score=550, Evalue=1e-156,
Organism=Homo sapiens, GI24307939, Length=357, Percent_Identity=21.5686274509804, Blast_Score=67, Evalue=6e-11,
Organism=Homo sapiens, GI38455427, Length=134, Percent_Identity=31.3432835820896, Blast_Score=66, Evalue=8e-11,
Organism=Escherichia coli, GI1790586, Length=530, Percent_Identity=76.0377358490566, Blast_Score=813, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17555558, Length=528, Percent_Identity=53.219696969697, Blast_Score=554, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI193210679, Length=217, Percent_Identity=51.6129032258064, Blast_Score=218, Evalue=7e-57,
Organism=Caenorhabditis elegans, GI17564182, Length=118, Percent_Identity=34.7457627118644, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI32566944, Length=118, Percent_Identity=34.7457627118644, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323288, Length=524, Percent_Identity=53.6259541984733, Blast_Score=549, Evalue=1e-157,
Organism=Saccharomyces cerevisiae, GI6322524, Length=558, Percent_Identity=22.4014336917563, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24641193, Length=527, Percent_Identity=51.9924098671727, Blast_Score=547, Evalue=1e-156,
Organism=Drosophila melanogaster, GI24641191, Length=527, Percent_Identity=51.9924098671727, Blast_Score=547, Evalue=1e-156,
Organism=Drosophila melanogaster, GI45550936, Length=529, Percent_Identity=50.6616257088847, Blast_Score=529, Evalue=1e-150,
Organism=Drosophila melanogaster, GI45550132, Length=529, Percent_Identity=50.6616257088847, Blast_Score=529, Evalue=1e-150,
Organism=Drosophila melanogaster, GI45550935, Length=529, Percent_Identity=50.6616257088847, Blast_Score=529, Evalue=1e-150,
Organism=Drosophila melanogaster, GI17864606, Length=553, Percent_Identity=43.5804701627486, Blast_Score=464, Evalue=1e-131,
Organism=Drosophila melanogaster, GI24584129, Length=526, Percent_Identity=37.2623574144487, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI19921262, Length=526, Percent_Identity=37.2623574144487, Blast_Score=337, Evalue=1e-92,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): CH60_NITMU (Q2Y6I6)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_413027.1
- HSSP:   P0A6F5
- ProteinModelPortal:   Q2Y6I6
- SMR:   Q2Y6I6
- STRING:   Q2Y6I6
- GeneID:   3784748
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A2344
- eggNOG:   COG0459
- HOGENOM:   HBG625289
- OMA:   NSDTSIG
- PhylomeDB:   Q2Y6I6
- ProtClustDB:   PRK00013
- BioCyc:   NMUL323848:NMUL_A2344-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00600
- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423
- PANTHER:   PTHR11353
- PRINTS:   PR00298
- TIGRFAMs:   TIGR02348

Pfam domain/function: PF00118 Cpn60_TCP1; SSF48592 GroEL-ATPase

EC number: NA

Molecular weight: Translated: 58195; Mature: 58063

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKEVKFSDSARHKMVNGVNILADAVKVTLGPKGRNVVLERSYGSPTITKDGVSVAKEI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECCCCCCCCCCCHHHHHHH
ELKDKFENMGAQMVKEVASKTSDVAGDGTTTATVLAQSIVKEGMKYVAAGMNPMDLKRGI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCH
DKAVVATVEELKKLSKPCTTGKEIAQVGSISANSDPEIGKIIADAMEKVGKEGVITVEDG
HHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECC
SGLQNELEVVEGMQFDRGYLSPYFINNADRQIALLESPFILLHDKKISNIRDLLPVLEQV
CCCHHHHHHHHCCCCCCCCCCCCEECCCCCEEEEECCCEEEEECCHHHHHHHHHHHHHHH
AKAGKPLLIIAEDVDGEALATLVVNNIRGILKTCAVKAPGFGDRRKAMLEDIAILTGGTV
HHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHH
IAEEVGLSLEKATLAELGQAKRVEVGKEETTIIDGAGDTQNIEGRVKQIRAQIEEATSDY
HHHHHCCCHHHHHHHHHCCCHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCC
DKEKLQERVAKLAGGVALIKVGAATEVEMKEKKARVEDALHATRAAVEEGIVPGGGVALL
CHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
RTRSAVSNLKGDNHDQDAGIKIVLRALEEPLRQIVANCGDEPSVVINKVLEGTENFGYNA
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCC
ASSEYGDMVQMGVLDPTKVTRYALQHAASIAGLMLTTDALVAEVPKEEGAGGGMGGGMGG
CCCHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCC
MGGMGGMGGMDM
CCCCCCCCCCCC
>Mature Secondary Structure 
AAKEVKFSDSARHKMVNGVNILADAVKVTLGPKGRNVVLERSYGSPTITKDGVSVAKEI
CCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECCCCCCCCCCCHHHHHHH
ELKDKFENMGAQMVKEVASKTSDVAGDGTTTATVLAQSIVKEGMKYVAAGMNPMDLKRGI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCH
DKAVVATVEELKKLSKPCTTGKEIAQVGSISANSDPEIGKIIADAMEKVGKEGVITVEDG
HHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECC
SGLQNELEVVEGMQFDRGYLSPYFINNADRQIALLESPFILLHDKKISNIRDLLPVLEQV
CCCHHHHHHHHCCCCCCCCCCCCEECCCCCEEEEECCCEEEEECCHHHHHHHHHHHHHHH
AKAGKPLLIIAEDVDGEALATLVVNNIRGILKTCAVKAPGFGDRRKAMLEDIAILTGGTV
HHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHH
IAEEVGLSLEKATLAELGQAKRVEVGKEETTIIDGAGDTQNIEGRVKQIRAQIEEATSDY
HHHHHCCCHHHHHHHHHCCCHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCC
DKEKLQERVAKLAGGVALIKVGAATEVEMKEKKARVEDALHATRAAVEEGIVPGGGVALL
CHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
RTRSAVSNLKGDNHDQDAGIKIVLRALEEPLRQIVANCGDEPSVVINKVLEGTENFGYNA
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCC
ASSEYGDMVQMGVLDPTKVTRYALQHAASIAGLMLTTDALVAEVPKEEGAGGGMGGGMGG
CCCHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCC
MGGMGGMGGMDM
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA