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The map label for this gene is prfC [H]

Identifier: 82703447

GI number: 82703447

Start: 2651060

End: 2652667

Strand: Reverse

Name: prfC [H]

Synonym: Nmul_A2330

Alternate gene names: 82703447

Gene position: 2652667-2651060 (Counterclockwise)

Preceding gene: 82703448

Following gene: 82703443

Centisome position: 83.31

GC content: 56.84

Gene sequence:

>1608_bases
ATGGTTGGTCAGGAAGTGGCGCGCAGGCGCACGTTTGCCATAATTTCTCACCCGGATGCCGGTAAAACGACACTCACGGA
AAAATTACTGTTATTTGCCGGCGCCATCCATATCGCCGGCAGCGTCAAGGCGCGGAAGGCGAGCCGCCACGCGACCTCCG
ACTGGATGGAGATCGAGAAGCAACGGGGCATTTCGGTTGCCAGCTCGGTCATGCAGATGGAGTATGGGGATTGTGTCATC
AATCTGCTCGATACGCCGGGTCATCAGGATTTCTCGGAGGATACTTACCGGGTGCTGACCGCAGTGGATGCCGCCCTCAT
GGTGATCGATGCCGGCAACGGCGTGGAAGCCCAGACGTTGCGCTTGCTGGAAGTGTGCCGCGCGCGTAACACCCCCATTA
TCACTTTTGTAAACAAGATGGACCGGGAAGTGCGCGAGCCACTCGATCTCATCGATGAGATCGAGCGAACACTGCGCATG
GCAGCGGTACCGTTTACGTGGCCCATGGGGATGGGGCGCGGTTTTCAAGGCGTCTTCGATCTGCGGCACAATCACATGCG
TGTTTTTCATCCCGGTTCTGACAGGCTGGACGATGAAGATGAGATCATCCAAGGCCTGGATAACCCCCTGTTGGCAGAAC
GTTTTGGAGACCAGGCCGCGAATCTGCAGCAGGAAATCGAGCTGATAAGAGGCGCCTCACCGGAATTCGACCAGGAAGCG
TTTCTCGCAGGCCGCCAGACTCCGGTTTTTTTCGGTTCGGCCATTAATAACTTCGGCATCCGTGAAGTGCTGGATGCCCT
GGTAGGTCTTGCCCCCCCCCCTGGTTCACGCAGCGCATTGCAGCGTCATGTCCAGCCGCATGAACTCCGGTTTACGGGGG
TAGTGTTCAAGATTCAGGCCAACATGAATCCAGCCCATCGGGATCGTATCGCCTTCGTTCGCATCTGTTCCGGCCGGTTC
GAACGCGGCATGAACCTGAGAATTGCGCGTAACGACAAGGATATTCGTACCAACGGGGTCGTATCTTTTCTCTCCCAACG
GCGCGACCTGCTCGACGTTGCCTATCCCGGAGATATCATTGGCATTCCCAATCATGGGACACTGCAACTCGGGGATACGT
TGACAGAAGGAGAGCGTTTGCAGTTTACCGGTCTGCCATTTTTCGCGCCGGAAATCTTTCAGACCGTGGAAATCGCCGAT
CCCATGCGCAGCAAGCAGCTCAAACTGGGGCTGGCGCAATTGGGCGAAGAGGGTGCAATCCAGGTTTTCAGGCCTCACCT
GGGCAGCGCGCTGCTGCTCGGCGCCGTTGGAGCTTTACAATTCGACGTTGTCGCACATCGCCTCAAGTATGAATACAGCG
TGGATGCACGTCTTGCTCCCGCGAAATACCAGCTGGCGCGATGGATTACCGCTGAGGATGCACGCGAACTCAAACGTTTC
ATCGACACCAACGCCCACCGCGTCGCTTATGATGCCGTGGGCGCACCGACATTCCTGGCCTCCTTTGGCGCGGAACTCAG
CGTGGCCGAAGAAAACTGGAAACACATTCGTTTCCACAAAATGCGCGAGCACGCCGGGCTTGTGTTTCAGGAGTCTGTCG
ATGTTTGA

Upstream 100 bases:

>100_bases
GCACATTCAGTTCAACGCATACGGCACACTGCTATCTTTATCCTCAAGACGAGGAGCAGTCAGACCGTAAAACACAGGCA
TAAGAGAAGGAATACCCCGA

Downstream 100 bases:

>100_bases
CAGCCCCGAAGCGGACCTCAGCGGATTTCCGCCAGCAGTCTACCCATCATCTGCTCCGCTTGGCTGAGGTATCCCCCGCC
GAACAGGTTGAGATGGTTCA

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 535; Mature: 535

Protein sequence:

>535_residues
MVGQEVARRRTFAIISHPDAGKTTLTEKLLLFAGAIHIAGSVKARKASRHATSDWMEIEKQRGISVASSVMQMEYGDCVI
NLLDTPGHQDFSEDTYRVLTAVDAALMVIDAGNGVEAQTLRLLEVCRARNTPIITFVNKMDREVREPLDLIDEIERTLRM
AAVPFTWPMGMGRGFQGVFDLRHNHMRVFHPGSDRLDDEDEIIQGLDNPLLAERFGDQAANLQQEIELIRGASPEFDQEA
FLAGRQTPVFFGSAINNFGIREVLDALVGLAPPPGSRSALQRHVQPHELRFTGVVFKIQANMNPAHRDRIAFVRICSGRF
ERGMNLRIARNDKDIRTNGVVSFLSQRRDLLDVAYPGDIIGIPNHGTLQLGDTLTEGERLQFTGLPFFAPEIFQTVEIAD
PMRSKQLKLGLAQLGEEGAIQVFRPHLGSALLLGAVGALQFDVVAHRLKYEYSVDARLAPAKYQLARWITAEDARELKRF
IDTNAHRVAYDAVGAPTFLASFGAELSVAEENWKHIRFHKMREHAGLVFQESVDV

Sequences:

>Translated_535_residues
MVGQEVARRRTFAIISHPDAGKTTLTEKLLLFAGAIHIAGSVKARKASRHATSDWMEIEKQRGISVASSVMQMEYGDCVI
NLLDTPGHQDFSEDTYRVLTAVDAALMVIDAGNGVEAQTLRLLEVCRARNTPIITFVNKMDREVREPLDLIDEIERTLRM
AAVPFTWPMGMGRGFQGVFDLRHNHMRVFHPGSDRLDDEDEIIQGLDNPLLAERFGDQAANLQQEIELIRGASPEFDQEA
FLAGRQTPVFFGSAINNFGIREVLDALVGLAPPPGSRSALQRHVQPHELRFTGVVFKIQANMNPAHRDRIAFVRICSGRF
ERGMNLRIARNDKDIRTNGVVSFLSQRRDLLDVAYPGDIIGIPNHGTLQLGDTLTEGERLQFTGLPFFAPEIFQTVEIAD
PMRSKQLKLGLAQLGEEGAIQVFRPHLGSALLLGAVGALQFDVVAHRLKYEYSVDARLAPAKYQLARWITAEDARELKRF
IDTNAHRVAYDAVGAPTFLASFGAELSVAEENWKHIRFHKMREHAGLVFQESVDV
>Mature_535_residues
MVGQEVARRRTFAIISHPDAGKTTLTEKLLLFAGAIHIAGSVKARKASRHATSDWMEIEKQRGISVASSVMQMEYGDCVI
NLLDTPGHQDFSEDTYRVLTAVDAALMVIDAGNGVEAQTLRLLEVCRARNTPIITFVNKMDREVREPLDLIDEIERTLRM
AAVPFTWPMGMGRGFQGVFDLRHNHMRVFHPGSDRLDDEDEIIQGLDNPLLAERFGDQAANLQQEIELIRGASPEFDQEA
FLAGRQTPVFFGSAINNFGIREVLDALVGLAPPPGSRSALQRHVQPHELRFTGVVFKIQANMNPAHRDRIAFVRICSGRF
ERGMNLRIARNDKDIRTNGVVSFLSQRRDLLDVAYPGDIIGIPNHGTLQLGDTLTEGERLQFTGLPFFAPEIFQTVEIAD
PMRSKQLKLGLAQLGEEGAIQVFRPHLGSALLLGAVGALQFDVVAHRLKYEYSVDARLAPAKYQLARWITAEDARELKRF
IDTNAHRVAYDAVGAPTFLASFGAELSVAEENWKHIRFHKMREHAGLVFQESVDV

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=508, Percent_Identity=26.3779527559055, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI19923640, Length=512, Percent_Identity=27.1484375, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI25306283, Length=456, Percent_Identity=27.6315789473684, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI25306287, Length=299, Percent_Identity=31.1036789297659, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI157426893, Length=154, Percent_Identity=31.8181818181818, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI94966754, Length=147, Percent_Identity=32.6530612244898, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI4503483, Length=152, Percent_Identity=32.2368421052632, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI310132016, Length=121, Percent_Identity=34.7107438016529, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI310110807, Length=121, Percent_Identity=34.7107438016529, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI310123363, Length=121, Percent_Identity=34.7107438016529, Blast_Score=70, Evalue=6e-12,
Organism=Escherichia coli, GI1790835, Length=522, Percent_Identity=52.6819923371648, Blast_Score=586, Evalue=1e-168,
Organism=Escherichia coli, GI1789738, Length=502, Percent_Identity=28.0876494023904, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1788922, Length=177, Percent_Identity=33.3333333333333, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI48994988, Length=184, Percent_Identity=30.9782608695652, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17533571, Length=495, Percent_Identity=27.0707070707071, Blast_Score=167, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17556745, Length=190, Percent_Identity=36.8421052631579, Blast_Score=128, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI17557151, Length=132, Percent_Identity=34.0909090909091, Blast_Score=89, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17506493, Length=165, Percent_Identity=32.1212121212121, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=33.0827067669173, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=33.0827067669173, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=483, Percent_Identity=26.9151138716356, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6322359, Length=530, Percent_Identity=23.3962264150943, Blast_Score=117, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6323320, Length=151, Percent_Identity=29.1390728476821, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324707, Length=158, Percent_Identity=31.6455696202532, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320593, Length=158, Percent_Identity=31.6455696202532, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24582462, Length=486, Percent_Identity=27.3662551440329, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI221458488, Length=506, Percent_Identity=26.4822134387352, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI78706572, Length=159, Percent_Identity=35.2201257861635, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24585711, Length=157, Percent_Identity=34.3949044585987, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24585713, Length=157, Percent_Identity=34.3949044585987, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24585709, Length=157, Percent_Identity=34.3949044585987, Blast_Score=79, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59707; Mature: 59707

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGQEVARRRTFAIISHPDAGKTTLTEKLLLFAGAIHIAGSVKARKASRHATSDWMEIEK
CCCHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHH
QRGISVASSVMQMEYGDCVINLLDTPGHQDFSEDTYRVLTAVDAALMVIDAGNGVEAQTL
HCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHEEEEEEECCCCCHHHHH
RLLEVCRARNTPIITFVNKMDREVREPLDLIDEIERTLRMAAVPFTWPMGMGRGFQGVFD
HHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
LRHNHMRVFHPGSDRLDDEDEIIQGLDNPLLAERFGDQAANLQQEIELIRGASPEFDQEA
HCCCCEEEECCCCCCCCCHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHH
FLAGRQTPVFFGSAINNFGIREVLDALVGLAPPPGSRSALQRHVQPHELRFTGVVFKIQA
HHCCCCCCEEECCHHCCCCHHHHHHHHHCCCCCCCCHHHHHHCCCCCEEEEEEEEEEEEE
NMNPAHRDRIAFVRICSGRFERGMNLRIARNDKDIRTNGVVSFLSQRRDLLDVAYPGDII
CCCHHHHHHHHHEEEHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHEEECCCCCEE
GIPNHGTLQLGDTLTEGERLQFTGLPFFAPEIFQTVEIADPMRSKQLKLGLAQLGEEGAI
ECCCCCCEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCE
QVFRPHLGSALLLGAVGALQFDVVAHRLKYEYSVDARLAPAKYQLARWITAEDARELKRF
EEECHHCCHHHHHHHHHHHHHHHHHHHHHCEECCCCCCCCHHHHHHHHHCHHHHHHHHHH
IDTNAHRVAYDAVGAPTFLASFGAELSVAEENWKHIRFHKMREHAGLVFQESVDV
HCCCCCEEEECCCCCHHHHHHCCCCEEECHHCCHHHHHHHHHHHCCEEEECCCCC
>Mature Secondary Structure
MVGQEVARRRTFAIISHPDAGKTTLTEKLLLFAGAIHIAGSVKARKASRHATSDWMEIEK
CCCHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHH
QRGISVASSVMQMEYGDCVINLLDTPGHQDFSEDTYRVLTAVDAALMVIDAGNGVEAQTL
HCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHEEEEEEECCCCCHHHHH
RLLEVCRARNTPIITFVNKMDREVREPLDLIDEIERTLRMAAVPFTWPMGMGRGFQGVFD
HHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
LRHNHMRVFHPGSDRLDDEDEIIQGLDNPLLAERFGDQAANLQQEIELIRGASPEFDQEA
HCCCCEEEECCCCCCCCCHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHH
FLAGRQTPVFFGSAINNFGIREVLDALVGLAPPPGSRSALQRHVQPHELRFTGVVFKIQA
HHCCCCCCEEECCHHCCCCHHHHHHHHHCCCCCCCCHHHHHHCCCCCEEEEEEEEEEEEE
NMNPAHRDRIAFVRICSGRFERGMNLRIARNDKDIRTNGVVSFLSQRRDLLDVAYPGDII
CCCHHHHHHHHHEEEHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHEEECCCCCEE
GIPNHGTLQLGDTLTEGERLQFTGLPFFAPEIFQTVEIADPMRSKQLKLGLAQLGEEGAI
ECCCCCCEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCE
QVFRPHLGSALLLGAVGALQFDVVAHRLKYEYSVDARLAPAKYQLARWITAEDARELKRF
EEECHHCCHHHHHHHHHHHHHHHHHHHHHCEECCCCCCCCHHHHHHHHHCHHHHHHHHHH
IDTNAHRVAYDAVGAPTFLASFGAELSVAEENWKHIRFHKMREHAGLVFQESVDV
HCCCCCEEEECCCCCHHHHHHCCCCEEECHHCCHHHHHHHHHHHCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA