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The map label for this gene is rlmB [H]

Identifier: 82703073

GI number: 82703073

Start: 2241846

End: 2242595

Strand: Reverse

Name: rlmB [H]

Synonym: Nmul_A1952

Alternate gene names: 82703073

Gene position: 2242595-2241846 (Counterclockwise)

Preceding gene: 82703074

Following gene: 82703072

Centisome position: 70.43

GC content: 59.73

Gene sequence:

>750_bases
ATGTCCGGCTCACGTTTTATATTCGGTTTTCATGCAATTACCAGTCGGTTGCGGCAGAATGCGGCCAGCGTCAGGGAGGT
TTTCCTCGATGCGGAGCGAAGAGATCAACGCGCCCGCGATCTGGCGGCGTTGGCGGAAGCCCAGGACATACGCTTGATTG
CATGCGACAATGCGAGGCTCATGGCTTTGGCGGGCAATGCCCGGCACCAGGGGGTAGTCGCGCGTATCGACTCCGCCCGG
GCGTATGTGGATATCGACGATGTGCTCGACACCCTCGCTGAGCCCGCGCTGCTTCTGGTGCTGGATGGTATCCAGGATCC
TCATAATCTCGGTGCCTGCCTGCGTGTTGCAGATGCTTTCGGTGTTCATGCTGTGATTGCGCCGAAAGACCGCGCGGTGG
GTCTCACCGCTGCCGTGCACAAGGTGGCAAGCGGCGCGGCCGATAACGTTCCCTATATTTCCGTGACGAATCTCGCCCGT
ACCCTGCGCGGATTGAAGCAGCGGGGAGTAACGGTCCTGGGAACGGCTGCCGATGCCGATACCCAGCTTGGCGTCCTCCA
GCTTGCTGGACCCATCGCATGGGTACTGGGTTCGGAAGAAAAAGGAATGCGGCGGCTGACGCGGGAAAACTGCGATCAGC
TTGTTTCGATTCCCATGCTGGGTAGCGTGGAAAGTCTCAACGTTTCCGTGAGCGCGGGTATTTGCCTGTTTGAAACGCAC
CGCCAGAGATATGCCCCCAAGGCCGGCTGA

Upstream 100 bases:

>100_bases
CCCAGGATGCGGGGAAAATAAAAACCGAGGCAGTGAGTTGATTTCCGCGATGAATGTCCAGTCCCCGCTTATCATTTTCT
GCAATTACTCGTTACTATAG

Downstream 100 bases:

>100_bases
CCCCTAGTTCGCGCCCTGCCTCACTTTTTCGCCGCAGCACGCACCGGGAAACAAATATTAACTCTTCTCCTATGACCCCG
ATCAAAATTGATATTTTCCA

Product: RNA methyltransferase TrmH

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MSGSRFIFGFHAITSRLRQNAASVREVFLDAERRDQRARDLAALAEAQDIRLIACDNARLMALAGNARHQGVVARIDSAR
AYVDIDDVLDTLAEPALLLVLDGIQDPHNLGACLRVADAFGVHAVIAPKDRAVGLTAAVHKVASGAADNVPYISVTNLAR
TLRGLKQRGVTVLGTAADADTQLGVLQLAGPIAWVLGSEEKGMRRLTRENCDQLVSIPMLGSVESLNVSVSAGICLFETH
RQRYAPKAG

Sequences:

>Translated_249_residues
MSGSRFIFGFHAITSRLRQNAASVREVFLDAERRDQRARDLAALAEAQDIRLIACDNARLMALAGNARHQGVVARIDSAR
AYVDIDDVLDTLAEPALLLVLDGIQDPHNLGACLRVADAFGVHAVIAPKDRAVGLTAAVHKVASGAADNVPYISVTNLAR
TLRGLKQRGVTVLGTAADADTQLGVLQLAGPIAWVLGSEEKGMRRLTRENCDQLVSIPMLGSVESLNVSVSAGICLFETH
RQRYAPKAG
>Mature_248_residues
SGSRFIFGFHAITSRLRQNAASVREVFLDAERRDQRARDLAALAEAQDIRLIACDNARLMALAGNARHQGVVARIDSARA
YVDIDDVLDTLAEPALLLVLDGIQDPHNLGACLRVADAFGVHAVIAPKDRAVGLTAAVHKVASGAADNVPYISVTNLART
LRGLKQRGVTVLGTAADADTQLGVLQLAGPIAWVLGSEEKGMRRLTRENCDQLVSIPMLGSVESLNVSVSAGICLFETHR
QRYAPKAG

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=258, Percent_Identity=30.6201550387597, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1790623, Length=242, Percent_Identity=49.5867768595041, Blast_Score=229, Evalue=1e-61,
Organism=Escherichia coli, GI1788935, Length=190, Percent_Identity=31.0526315789474, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1790083, Length=154, Percent_Identity=31.8181818181818, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26597; Mature: 26466

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGSRFIFGFHAITSRLRQNAASVREVFLDAERRDQRARDLAALAEAQDIRLIACDNARL
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCEEEEEECCCEE
MALAGNARHQGVVARIDSARAYVDIDDVLDTLAEPALLLVLDGIQDPHNLGACLRVADAF
EEEECCCCCCCEEEEECCCCEEEEHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHH
GVHAVIAPKDRAVGLTAAVHKVASGAADNVPYISVTNLARTLRGLKQRGVTVLGTAADAD
CEEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCEEEEECCCCC
TQLGVLQLAGPIAWVLGSEEKGMRRLTRENCDQLVSIPMLGSVESLNVSVSAGICLFETH
CCCHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCEEEEEHH
RQRYAPKAG
HHHCCCCCC
>Mature Secondary Structure 
SGSRFIFGFHAITSRLRQNAASVREVFLDAERRDQRARDLAALAEAQDIRLIACDNARL
CCCEEEEHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCEEEEEECCCEE
MALAGNARHQGVVARIDSARAYVDIDDVLDTLAEPALLLVLDGIQDPHNLGACLRVADAF
EEEECCCCCCCEEEEECCCCEEEEHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHH
GVHAVIAPKDRAVGLTAAVHKVASGAADNVPYISVTNLARTLRGLKQRGVTVLGTAADAD
CEEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCEEEEECCCCC
TQLGVLQLAGPIAWVLGSEEKGMRRLTRENCDQLVSIPMLGSVESLNVSVSAGICLFETH
CCCHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCEEEEEHH
RQRYAPKAG
HHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]