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The map label for this gene is dnaB [H]

Identifier: 82703068

GI number: 82703068

Start: 2238686

End: 2240098

Strand: Reverse

Name: dnaB [H]

Synonym: Nmul_A1947

Alternate gene names: 82703068

Gene position: 2240098-2238686 (Counterclockwise)

Preceding gene: 82703069

Following gene: 82703064

Centisome position: 70.35

GC content: 55.27

Gene sequence:

>1413_bases
ATGCCTGTAACGTCACGTAACTCCATGCCTCAATTTGTAACGGCAGCTTCAAACGAGCAATTACTCGAATCATACAAATT
GCCCCCGCATTCGGTTGAAGCAGAGCAATCGGTATTGGGCGGATTGATGCTGGACAATCACGCATGGGATAAGGTAGCGG
ACGTGATCACGGAAGATGACTTCTACCGGCAGGATCACAAGCTCATCTACCGCCATATCTGCAAACTGGTCGAGCACAGC
AAACCGGCCGATGTCATCACGGTAGCGGAATCACTTGAAATATCCGCGGAATTGCAGGGGGCTGGGGGGCTCGCTTACGT
GGGGATCCTCGTACAGAATACGCCTTCGGCTGCAAATATCCGCCGCTATGCGGAAATCGTGCGCGAGCGCGCAATCATGC
GCAAGCTTGCCCAGGTGGGGACCGACATCACGGATTCGGCATACAACCCTGCTGGGCGTTCAGCCGCCACGCTGCTGGAT
GAAGCCGAGGCCAGGGTTTTTGAAATTGCCGAGGCAGGTGCGCGAGGCAAGCAGGGTTTTATCGACATTCAGCCGCTGCT
GAAGGAGGTCGTGGAGCGTATAGAGACGCTCTACAACCAGGATAATCAAAGCGATGTTACCGGAATCGCAACCGGCTTCC
ATGATCTGGATGAAAAGACTTCGGGCTTTCAGCCGGGGGATCTTGTCATTGTTGCGGGTCGGCCATCGATGGGCAAAACC
GCCTTTTCGCTCAATATTGCCGAACATGTTGCACTCACGCTCCAAAAACCGGTAGCGGTATTCAGCATGGAAATGGGAGG
CGCGCAACTGGCGATGCGTATGCTGGGCTCCGTCGGAAAGCTGGATCAGCACAAGGTGCGTACCGGCCGCCTGCTGGACG
AGGATTGGTCCCGCCTGACGCATGCGCTTGGCAAGCTGAACGACGCTCCGCTTTTTATTGACGAAAGTGCCGCTTTGAAT
GCCCTGGAATTAAGGGCGCGCGCGCGCCGGCTGCATCGCCAGCATGGGGAACTGGGCTTGATCGTGGTGGATTATCTCCA
GTTGATGTCCGCCGCCGGCCAGGGCGAGAACCGCGCAACGGAGATTTCCGAAATATCCCGCTCCCTCAAGGCACTGGCAA
AAGAACTGCATGTGCCCGTTGTCGCGTTATCCCAGTTGAATCGAAGTCTTGAGCAGCGTCCGAACAAGCGTCCCGTTATG
TCCGATCTGCGCGAGTCCGGTGCGATCGAACAGGATGCCGATCTGATTCTTTTCATCTATCGGGACGAAGTTTATAACCC
GGAGACACCGGACAAGGGAATCGCCGAAATCATTATAGGAAAGCAGAGGAATGGACCGATCGGCAAGGTCGATCTGACTT
TTCTGGGCGAATATACCCGATTCGAAAGCTACGCCAGAGCAGGTCATTATTGA

Upstream 100 bases:

>100_bases
ATCGGTCTCCTGAAAAGGGGTCTGTCTTTTGATTTTCAAAAGGGGCTGGCAACAGCCCTTTTTGATTTCTGGCTCATTGC
AACAGTGTTAAAATCCAGAC

Downstream 100 bases:

>100_bases
GACTACGGTTCAACTATCTTTCGCTATTGTCAGGCTAAACTTTCGGGAGTGCAGGTCAGGGCCGCCTGACGACCACGACT
TATCCTGCTCGCTTGACCCA

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MPVTSRNSMPQFVTAASNEQLLESYKLPPHSVEAEQSVLGGLMLDNHAWDKVADVITEDDFYRQDHKLIYRHICKLVEHS
KPADVITVAESLEISAELQGAGGLAYVGILVQNTPSAANIRRYAEIVRERAIMRKLAQVGTDITDSAYNPAGRSAATLLD
EAEARVFEIAEAGARGKQGFIDIQPLLKEVVERIETLYNQDNQSDVTGIATGFHDLDEKTSGFQPGDLVIVAGRPSMGKT
AFSLNIAEHVALTLQKPVAVFSMEMGGAQLAMRMLGSVGKLDQHKVRTGRLLDEDWSRLTHALGKLNDAPLFIDESAALN
ALELRARARRLHRQHGELGLIVVDYLQLMSAAGQGENRATEISEISRSLKALAKELHVPVVALSQLNRSLEQRPNKRPVM
SDLRESGAIEQDADLILFIYRDEVYNPETPDKGIAEIIIGKQRNGPIGKVDLTFLGEYTRFESYARAGHY

Sequences:

>Translated_470_residues
MPVTSRNSMPQFVTAASNEQLLESYKLPPHSVEAEQSVLGGLMLDNHAWDKVADVITEDDFYRQDHKLIYRHICKLVEHS
KPADVITVAESLEISAELQGAGGLAYVGILVQNTPSAANIRRYAEIVRERAIMRKLAQVGTDITDSAYNPAGRSAATLLD
EAEARVFEIAEAGARGKQGFIDIQPLLKEVVERIETLYNQDNQSDVTGIATGFHDLDEKTSGFQPGDLVIVAGRPSMGKT
AFSLNIAEHVALTLQKPVAVFSMEMGGAQLAMRMLGSVGKLDQHKVRTGRLLDEDWSRLTHALGKLNDAPLFIDESAALN
ALELRARARRLHRQHGELGLIVVDYLQLMSAAGQGENRATEISEISRSLKALAKELHVPVVALSQLNRSLEQRPNKRPVM
SDLRESGAIEQDADLILFIYRDEVYNPETPDKGIAEIIIGKQRNGPIGKVDLTFLGEYTRFESYARAGHY
>Mature_469_residues
PVTSRNSMPQFVTAASNEQLLESYKLPPHSVEAEQSVLGGLMLDNHAWDKVADVITEDDFYRQDHKLIYRHICKLVEHSK
PADVITVAESLEISAELQGAGGLAYVGILVQNTPSAANIRRYAEIVRERAIMRKLAQVGTDITDSAYNPAGRSAATLLDE
AEARVFEIAEAGARGKQGFIDIQPLLKEVVERIETLYNQDNQSDVTGIATGFHDLDEKTSGFQPGDLVIVAGRPSMGKTA
FSLNIAEHVALTLQKPVAVFSMEMGGAQLAMRMLGSVGKLDQHKVRTGRLLDEDWSRLTHALGKLNDAPLFIDESAALNA
LELRARARRLHRQHGELGLIVVDYLQLMSAAGQGENRATEISEISRSLKALAKELHVPVVALSQLNRSLEQRPNKRPVMS
DLRESGAIEQDADLILFIYRDEVYNPETPDKGIAEIIIGKQRNGPIGKVDLTFLGEYTRFESYARAGHY

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=441, Percent_Identity=56.9160997732426, Blast_Score=513, Evalue=1e-146,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 51828; Mature: 51696

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVTSRNSMPQFVTAASNEQLLESYKLPPHSVEAEQSVLGGLMLDNHAWDKVADVITEDD
CCCCCCCCCCCHHHHCCHHHHHHHHCCCCCCCCHHHHHHCCHHCCCCHHHHHHHHHCCCH
FYRQDHKLIYRHICKLVEHSKPADVITVAESLEISAELQGAGGLAYVGILVQNTPSAANI
HHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHEEEEEECCCCCEEEEEEEEECCCCHHHH
RRYAEIVRERAIMRKLAQVGTDITDSAYNPAGRSAATLLDEAEARVFEIAEAGARGKQGF
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
IDIQPLLKEVVERIETLYNQDNQSDVTGIATGFHDLDEKTSGFQPGDLVIVAGRPSMGKT
EEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCHHHHHHHCCCCCCCCEEEEECCCCCCCC
AFSLNIAEHVALTLQKPVAVFSMEMGGAQLAMRMLGSVGKLDQHKVRTGRLLDEDWSRLT
EEEEEHHHHHHHHHHCCHHHEEECCCHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHH
HALGKLNDAPLFIDESAALNALELRARARRLHRQHGELGLIVVDYLQLMSAAGQGENRAT
HHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHCCCCCCHHH
EISEISRSLKALAKELHVPVVALSQLNRSLEQRPNKRPVMSDLRESGAIEQDADLILFIY
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEE
RDEVYNPETPDKGIAEIIIGKQRNGPIGKVDLTFLGEYTRFESYARAGHY
ECCCCCCCCCCCCHHHHHEECCCCCCCCEEEHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PVTSRNSMPQFVTAASNEQLLESYKLPPHSVEAEQSVLGGLMLDNHAWDKVADVITEDD
CCCCCCCCCCHHHHCCHHHHHHHHCCCCCCCCHHHHHHCCHHCCCCHHHHHHHHHCCCH
FYRQDHKLIYRHICKLVEHSKPADVITVAESLEISAELQGAGGLAYVGILVQNTPSAANI
HHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHEEEEEECCCCCEEEEEEEEECCCCHHHH
RRYAEIVRERAIMRKLAQVGTDITDSAYNPAGRSAATLLDEAEARVFEIAEAGARGKQGF
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
IDIQPLLKEVVERIETLYNQDNQSDVTGIATGFHDLDEKTSGFQPGDLVIVAGRPSMGKT
EEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCHHHHHHHCCCCCCCCEEEEECCCCCCCC
AFSLNIAEHVALTLQKPVAVFSMEMGGAQLAMRMLGSVGKLDQHKVRTGRLLDEDWSRLT
EEEEEHHHHHHHHHHCCHHHEEECCCHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHH
HALGKLNDAPLFIDESAALNALELRARARRLHRQHGELGLIVVDYLQLMSAAGQGENRAT
HHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHCCCCCCHHH
EISEISRSLKALAKELHVPVVALSQLNRSLEQRPNKRPVMSDLRESGAIEQDADLILFIY
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEE
RDEVYNPETPDKGIAEIIIGKQRNGPIGKVDLTFLGEYTRFESYARAGHY
ECCCCCCCCCCCCHHHHHEECCCCCCCCEEEHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]