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The map label for this gene is trpA

Identifier: 82703032

GI number: 82703032

Start: 2201415

End: 2202254

Strand: Reverse

Name: trpA

Synonym: Nmul_A1911

Alternate gene names: 82703032

Gene position: 2202254-2201415 (Counterclockwise)

Preceding gene: 82703033

Following gene: 82703031

Centisome position: 69.16

GC content: 54.64

Gene sequence:

>840_bases
ATGTCCCGTATTTCCACCCTGTTTGGCAAACTGCTCCAGAATCGGAGAAAGGCGCTCATTCCTTTTATTACTGCTGGCGA
TCCCGAACCCGGAATGATGGTTCCACTGATGCATGAACTGGTGCAGGCTGGCGCCGATGTCATCGAACTGGGTGTTCCAT
TTTCTGATCCGATGGCGGATGGACCAACGATTCAGCGCTCTTCCGAGCGTGCGCTGAAACATCGCGTTTCGTTGCAGGAC
GTATTGGCAATGGTGGGAGAATTCAGGGAAAGTGATTCCAGCACGCCGGTTGTGCTGATGGGCTACGCCAATCCGGTCGA
AGCGATGGGCTACGATGCTTTCACGGCAAGATCAAAGGCGTGCGGAGTGGATGGCGTGCTGATTGTGGATTATCCTCCGG
AAGAATCGGTGAAATGGGTGGAATACCTGAAACGTCAGAATATCGCTCCCATTTTCCTGCTTTCTCCCACTACGCCGCAG
CAGCGCGTCGAGCGGGTGGCCTCCCTGGCGGAAGGATACGTGTACTACGTTTCGCTGAAAGGCGTTACCGGTTCGCTTCA
CCTCGATTTGCATGACGTGGCCGAGAAACTCGACGGACTGCGTTCCCGCATTTCCATTCCTATCGGAGTGGGCTTTGGCA
TACGTGACGGCGCAACCGCCCGGGCAGTGGCGGAGCTTGCCGATGCAGTGGTGGTGGGAAGCCGCATCATCGAAGAAATT
GAAAGGTCGCCCAAGGCGGAGGTGTTAACAAATGTCCATCGTCTGGTAAAAAGTCTGCGAATTGCAATAGACGCAGCAAG
TCCTGCACCATCAGCCGCCGTTAAACTTACACACTTATGA

Upstream 100 bases:

>100_bases
GACAAGTTGCTGCTGGTGAACCTCTCGGGGCGTGGGGACAAGGATATGCCCACCGTGGCGCGCGCTTCGCATATCACCTT
CTAACCTGCAAAGTCCATTC

Downstream 100 bases:

>100_bases
GCTGGTTTCAGAAACTTCTTCCGCCCAAGATCAAGCGCAAGGAAAGCGGTGAAAAGAAAGCGGTGCCTGAGGGACTGTGG
AGCAAATGCGCCTCCTGCGA

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MSRISTLFGKLLQNRRKALIPFITAGDPEPGMMVPLMHELVQAGADVIELGVPFSDPMADGPTIQRSSERALKHRVSLQD
VLAMVGEFRESDSSTPVVLMGYANPVEAMGYDAFTARSKACGVDGVLIVDYPPEESVKWVEYLKRQNIAPIFLLSPTTPQ
QRVERVASLAEGYVYYVSLKGVTGSLHLDLHDVAEKLDGLRSRISIPIGVGFGIRDGATARAVAELADAVVVGSRIIEEI
ERSPKAEVLTNVHRLVKSLRIAIDAASPAPSAAVKLTHL

Sequences:

>Translated_279_residues
MSRISTLFGKLLQNRRKALIPFITAGDPEPGMMVPLMHELVQAGADVIELGVPFSDPMADGPTIQRSSERALKHRVSLQD
VLAMVGEFRESDSSTPVVLMGYANPVEAMGYDAFTARSKACGVDGVLIVDYPPEESVKWVEYLKRQNIAPIFLLSPTTPQ
QRVERVASLAEGYVYYVSLKGVTGSLHLDLHDVAEKLDGLRSRISIPIGVGFGIRDGATARAVAELADAVVVGSRIIEEI
ERSPKAEVLTNVHRLVKSLRIAIDAASPAPSAAVKLTHL
>Mature_278_residues
SRISTLFGKLLQNRRKALIPFITAGDPEPGMMVPLMHELVQAGADVIELGVPFSDPMADGPTIQRSSERALKHRVSLQDV
LAMVGEFRESDSSTPVVLMGYANPVEAMGYDAFTARSKACGVDGVLIVDYPPEESVKWVEYLKRQNIAPIFLLSPTTPQQ
RVERVASLAEGYVYYVSLKGVTGSLHLDLHDVAEKLDGLRSRISIPIGVGFGIRDGATARAVAELADAVVVGSRIIEEIE
RSPKAEVLTNVHRLVKSLRIAIDAASPAPSAAVKLTHL

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=217, Percent_Identity=38.2488479262673, Blast_Score=152, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6321412, Length=250, Percent_Identity=37.2, Blast_Score=170, Evalue=2e-43,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_NITMU (Q2Y7R5)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_412598.1
- HSSP:   P00929
- ProteinModelPortal:   Q2Y7R5
- SMR:   Q2Y7R5
- STRING:   Q2Y7R5
- GeneID:   3784149
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A1911
- eggNOG:   COG0159
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- PhylomeDB:   Q2Y7R5
- ProtClustDB:   PRK13111
- BioCyc:   NMUL323848:NMUL_A1911-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 30178; Mature: 30047

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 49-49 ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRISTLFGKLLQNRRKALIPFITAGDPEPGMMVPLMHELVQAGADVIELGVPFSDPMAD
CCHHHHHHHHHHHHCHHHCCEEEECCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCC
GPTIQRSSERALKHRVSLQDVLAMVGEFRESDSSTPVVLMGYANPVEAMGYDAFTARSKA
CCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHCCCHHHHCCCC
CGVDGVLIVDYPPEESVKWVEYLKRQNIAPIFLLSPTTPQQRVERVASLAEGYVYYVSLK
CCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCEEEEEEEE
GVTGSLHLDLHDVAEKLDGLRSRISIPIGVGFGIRDGATARAVAELADAVVVGSRIIEEI
CCCEEEEECHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ERSPKAEVLTNVHRLVKSLRIAIDAASPAPSAAVKLTHL
HCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEEECC
>Mature Secondary Structure 
SRISTLFGKLLQNRRKALIPFITAGDPEPGMMVPLMHELVQAGADVIELGVPFSDPMAD
CHHHHHHHHHHHHCHHHCCEEEECCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCC
GPTIQRSSERALKHRVSLQDVLAMVGEFRESDSSTPVVLMGYANPVEAMGYDAFTARSKA
CCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHCCCHHHHCCCC
CGVDGVLIVDYPPEESVKWVEYLKRQNIAPIFLLSPTTPQQRVERVASLAEGYVYYVSLK
CCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCEEEEEEEE
GVTGSLHLDLHDVAEKLDGLRSRISIPIGVGFGIRDGATARAVAELADAVVVGSRIIEEI
CCCEEEEECHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ERSPKAEVLTNVHRLVKSLRIAIDAASPAPSAAVKLTHL
HCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA