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The map label for this gene is cysS

Identifier: 82703002

GI number: 82703002

Start: 2166601

End: 2167995

Strand: Reverse

Name: cysS

Synonym: Nmul_A1881

Alternate gene names: 82703002

Gene position: 2167995-2166601 (Counterclockwise)

Preceding gene: 82703009

Following gene: 82703001

Centisome position: 68.09

GC content: 54.27

Gene sequence:

>1395_bases
ATGCTTAAAATTCATAATTCTCTTGCCAGAGAAAAACAGGAGTTTATCCCGCTAACGCCGGGCAAGGTCAAAGTATATGT
ATGCGGAATGACCGTCTACGATTACTGCCATCTCGGCCATGCGCGCGTGATGGTGGTATTCGATATGGTGCAGCGCTGGT
TTCGCGCCAACCGTTTTGATGTGACATATGTACGTAATATTACCGATATTGATGACAAAATTATCAGGCGGGCGCAGGAA
AATAAGGAATCCATTGAAGCGCTGACGGCGCGTTTTATCCAGGCGATGGAAGAGGATGCCGCAGCGCTTGGGGTGGAGCC
GCCTTCATGGGAACCGCGTGCCACGCGCCATGTGGAAGACATGATTGCCATGATCCAGGTTCTGGTGGATAAAAAGCTGG
CTTATCCCGCTTCCAACGGCGACGTATATTATGCGGTTCACCGGTTTCCCGGTTATGGAAAGCTTTCCGGGAAATCCCTT
GACGAGTTGCGGGCAGGGGAACGGGTAATTGTCGATCCGCATAAAAACGATCCGCTGGATTTTGTGCTGTGGAAAGCGGC
AAAGCCCGGCGAGCCCCAGTGGGATTCCCCGTGGGGACCGGGGCGGCCCGGCTGGCATATCGAGTGCTCGGCCATGGGCG
AGCACTATCTGGGGGAGCACTTCGACATCCATGGCGGTGGGCAGGACTTACAGTTTCCCCATCATGAAAATGAAATTGCA
CAAAGCGAGGGGGCGCACGGGCATCCTTTTGTCAACTACTGGATGCACAATGGTTTCGTGCGAGTCAATGACGAGAAGAT
GTCCAAGTCGCTCGGCAATTTTTTTACCGTCCGCGAAATCCTTGCCCACTTTCAGCCGGAGGTGGTACGTTTTTTTATCC
TGCGCGCGCATTACCGCAGCCCGCTTAACTATGCAGACATGCAACTGGAAGACGCCGGGCGAGCTCTCGATCGACTGTAT
ACCGCGCTCAAGGGAATCGAAGTCCACGACGAAGTCGAGATAGACTGGGATGACCCCTATGCATCGCGTTTCCGGGAAGC
GATGAACGATGACTTCAACACCCCGGAGGCTCTTGCCGTGCTTTTCGACCTCGCTACCGAAGTCAATAAAACCCGACTAC
CCAGGGATATTGCCTTGATGAAAGCGCTGGGCGGGGTGCTGGGATTGCTCCAGCAGGACCCCTGGAAATATTTCCAGCAG
GCGGATTCCGAAGATCCGGCATTTGCGCCCGAAAAGGTGGAGCAATTGATTCAACAGCGGCTGGTTGCACGCGCCAACAA
GGATTATCGCGAGGCCGATCGCATTCGCAAGGAATTGATGGAGGCCAACATCATTCTGGAAGACGGACCTCATGGCACTA
CCTGGCGGAGGGCAACGGGTCTATCCACTCTCTGA

Upstream 100 bases:

>100_bases
CAGCGTTTTCATTGTGCTATACTTCTCCGTCCTGTAAGTAGTGCCCTCAGCGTCCGCAAGATTCTACCAAGAAGCCTGTT
CAATTCAAAATCTTCCCCAG

Downstream 100 bases:

>100_bases
GTGCGTCATGGACTGACGGGATGGGAGTCATGGCTCGCCTTATTTGCCGGGCCAAGTCCTCCACATCGGCTATCCTTTCG
ATATAAGTATAAATATGAAG

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MLKIHNSLAREKQEFIPLTPGKVKVYVCGMTVYDYCHLGHARVMVVFDMVQRWFRANRFDVTYVRNITDIDDKIIRRAQE
NKESIEALTARFIQAMEEDAAALGVEPPSWEPRATRHVEDMIAMIQVLVDKKLAYPASNGDVYYAVHRFPGYGKLSGKSL
DELRAGERVIVDPHKNDPLDFVLWKAAKPGEPQWDSPWGPGRPGWHIECSAMGEHYLGEHFDIHGGGQDLQFPHHENEIA
QSEGAHGHPFVNYWMHNGFVRVNDEKMSKSLGNFFTVREILAHFQPEVVRFFILRAHYRSPLNYADMQLEDAGRALDRLY
TALKGIEVHDEVEIDWDDPYASRFREAMNDDFNTPEALAVLFDLATEVNKTRLPRDIALMKALGGVLGLLQQDPWKYFQQ
ADSEDPAFAPEKVEQLIQQRLVARANKDYREADRIRKELMEANIILEDGPHGTTWRRATGLSTL

Sequences:

>Translated_464_residues
MLKIHNSLAREKQEFIPLTPGKVKVYVCGMTVYDYCHLGHARVMVVFDMVQRWFRANRFDVTYVRNITDIDDKIIRRAQE
NKESIEALTARFIQAMEEDAAALGVEPPSWEPRATRHVEDMIAMIQVLVDKKLAYPASNGDVYYAVHRFPGYGKLSGKSL
DELRAGERVIVDPHKNDPLDFVLWKAAKPGEPQWDSPWGPGRPGWHIECSAMGEHYLGEHFDIHGGGQDLQFPHHENEIA
QSEGAHGHPFVNYWMHNGFVRVNDEKMSKSLGNFFTVREILAHFQPEVVRFFILRAHYRSPLNYADMQLEDAGRALDRLY
TALKGIEVHDEVEIDWDDPYASRFREAMNDDFNTPEALAVLFDLATEVNKTRLPRDIALMKALGGVLGLLQQDPWKYFQQ
ADSEDPAFAPEKVEQLIQQRLVARANKDYREADRIRKELMEANIILEDGPHGTTWRRATGLSTL
>Mature_464_residues
MLKIHNSLAREKQEFIPLTPGKVKVYVCGMTVYDYCHLGHARVMVVFDMVQRWFRANRFDVTYVRNITDIDDKIIRRAQE
NKESIEALTARFIQAMEEDAAALGVEPPSWEPRATRHVEDMIAMIQVLVDKKLAYPASNGDVYYAVHRFPGYGKLSGKSL
DELRAGERVIVDPHKNDPLDFVLWKAAKPGEPQWDSPWGPGRPGWHIECSAMGEHYLGEHFDIHGGGQDLQFPHHENEIA
QSEGAHGHPFVNYWMHNGFVRVNDEKMSKSLGNFFTVREILAHFQPEVVRFFILRAHYRSPLNYADMQLEDAGRALDRLY
TALKGIEVHDEVEIDWDDPYASRFREAMNDDFNTPEALAVLFDLATEVNKTRLPRDIALMKALGGVLGLLQQDPWKYFQQ
ADSEDPAFAPEKVEQLIQQRLVARANKDYREADRIRKELMEANIILEDGPHGTTWRRATGLSTL

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=384, Percent_Identity=39.0625, Blast_Score=252, Evalue=5e-67,
Organism=Homo sapiens, GI10835051, Length=228, Percent_Identity=41.6666666666667, Blast_Score=176, Evalue=5e-44,
Organism=Homo sapiens, GI21269877, Length=228, Percent_Identity=41.6666666666667, Blast_Score=176, Evalue=6e-44,
Organism=Homo sapiens, GI62240992, Length=228, Percent_Identity=41.6666666666667, Blast_Score=176, Evalue=6e-44,
Organism=Homo sapiens, GI303304987, Length=228, Percent_Identity=41.6666666666667, Blast_Score=175, Evalue=7e-44,
Organism=Escherichia coli, GI1786737, Length=464, Percent_Identity=54.3103448275862, Blast_Score=488, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI17509797, Length=233, Percent_Identity=39.0557939914163, Blast_Score=169, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17509795, Length=233, Percent_Identity=39.0557939914163, Blast_Score=169, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6324082, Length=328, Percent_Identity=33.2317073170732, Blast_Score=192, Evalue=9e-50,
Organism=Drosophila melanogaster, GI19922218, Length=387, Percent_Identity=37.4677002583979, Blast_Score=242, Evalue=4e-64,
Organism=Drosophila melanogaster, GI19922364, Length=363, Percent_Identity=33.8842975206612, Blast_Score=178, Evalue=8e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_NITMU (Q2Y7U5)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_412568.1
- ProteinModelPortal:   Q2Y7U5
- SMR:   Q2Y7U5
- STRING:   Q2Y7U5
- GeneID:   3786531
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A1881
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- PhylomeDB:   Q2Y7U5
- ProtClustDB:   PRK00260
- BioCyc:   NMUL323848:NMUL_A1881-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 53253; Mature: 53253

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 269-269

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKIHNSLAREKQEFIPLTPGKVKVYVCGMTVYDYCHLGHARVMVVFDMVQRWFRANRFD
CCCCCHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCC
VTYVRNITDIDDKIIRRAQENKESIEALTARFIQAMEEDAAALGVEPPSWEPRATRHVED
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
MIAMIQVLVDKKLAYPASNGDVYYAVHRFPGYGKLSGKSLDELRAGERVIVDPHKNDPLD
HHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHCCCEEEECCCCCCCCE
FVLWKAAKPGEPQWDSPWGPGRPGWHIECSAMGEHYLGEHFDIHGGGQDLQFPHHENEIA
EEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEECCCCCCCCCCCCCCHHH
QSEGAHGHPFVNYWMHNGFVRVNDEKMSKSLGNFFTVREILAHFQPEVVRFFILRAHYRS
HCCCCCCCCHHHHEEECCEEEECHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCC
PLNYADMQLEDAGRALDRLYTALKGIEVHDEVEIDWDDPYASRFREAMNDDFNTPEALAV
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHCCCCCCHHHHHH
LFDLATEVNKTRLPRDIALMKALGGVLGLLQQDPWKYFQQADSEDPAFAPEKVEQLIQQR
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHHHH
LVARANKDYREADRIRKELMEANIILEDGPHGTTWRRATGLSTL
HHHHCCCCHHHHHHHHHHHHHCCEEEECCCCCCCCHHCCCCCCC
>Mature Secondary Structure
MLKIHNSLAREKQEFIPLTPGKVKVYVCGMTVYDYCHLGHARVMVVFDMVQRWFRANRFD
CCCCCHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCC
VTYVRNITDIDDKIIRRAQENKESIEALTARFIQAMEEDAAALGVEPPSWEPRATRHVED
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
MIAMIQVLVDKKLAYPASNGDVYYAVHRFPGYGKLSGKSLDELRAGERVIVDPHKNDPLD
HHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHCCCEEEECCCCCCCCE
FVLWKAAKPGEPQWDSPWGPGRPGWHIECSAMGEHYLGEHFDIHGGGQDLQFPHHENEIA
EEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEECCCCCCCCCCCCCCHHH
QSEGAHGHPFVNYWMHNGFVRVNDEKMSKSLGNFFTVREILAHFQPEVVRFFILRAHYRS
HCCCCCCCCHHHHEEECCEEEECHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCC
PLNYADMQLEDAGRALDRLYTALKGIEVHDEVEIDWDDPYASRFREAMNDDFNTPEALAV
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHCCCCCCHHHHHH
LFDLATEVNKTRLPRDIALMKALGGVLGLLQQDPWKYFQQADSEDPAFAPEKVEQLIQQR
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHHHH
LVARANKDYREADRIRKELMEANIILEDGPHGTTWRRATGLSTL
HHHHCCCCHHHHHHHHHHHHHCCEEEECCCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA