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The map label for this gene is glgX [H]

Identifier: 82702530

GI number: 82702530

Start: 1597609

End: 1599714

Strand: Direct

Name: glgX [H]

Synonym: Nmul_A1401

Alternate gene names: 82702530

Gene position: 1597609-1599714 (Clockwise)

Preceding gene: 82702529

Following gene: 82702538

Centisome position: 50.17

GC content: 55.18

Gene sequence:

>2106_bases
ATGAAAATCTGGCCAGGCACTCCCTACCCCCTTGGTGCGACATACGATGGAGCGGGAACCAATTTCTCCCTGTTTTCCGA
GGCGGCGGAACGCGTCGAACTATGCCTGTTCGATGAAGCCGGTCGTGAAACCAGGGTGAATTTGCCCGAGGTGACAGGAT
ACTGCTGGCACGGCTACCTGCCGGGCGTAGAGCCTGGACAGCGTTACGGTTTTCGGGTGCATGGGCCGTGGTCCCCGGAG
CAAGGTAATCGCTGTAATTCCGCGAAGCTGCTGCTCGATCCGTATGCAAAAGGGATCGATGGCGACATTACCTGGGATGA
AGCTGTATTCCCCTATCTTTTCGATGACCGCGATGCACGGAATGACAAGGATAGCGCGCCTTTCATGCCCCGCAGCATCG
TTCATCAGCCGTTTTTCGATTGGTCCGGCGATCGTCAACTCCAGCGCCCCTGGCACGAAACAGTCATTTATGAGTTGCAT
GTCAAGGGCTTCACCGCACAGCATCCCGACATACCCCCTGAACTGCGGGGCACTTATGCGGGGCTCGCACACTCTGCCTC
TATCGATTATCTGAAACAGCTTGGGGTCACGGCTGTCGAGCTGTTGCCAGTGCACTACTTTGTGCAGGACAAACATCTCC
TGGATCAGGGTTTGCGCAACTACTGGGGTTATAACTCCATTGCCTATTTTGCTCCCCATAGCGCCTACGCCGCGGACAAG
CGTCCGGGAGCCGCAAGCGCCGAATTCAAGCAAATGGTCAAGGCCCTGCATCAAGCCGACATAGAAGTTATCCTCGATGT
GGTGTACAACCATACTGCGGAAGGCAACGATCTCGGGCCGGTGTTATGCCTGAAGGGAATCGACAACGCCTATTATTACC
AGCTCATGGAAGATCGGCGCTACTACAGGGATTACACAGGCACAGGCAACTGCCTCAATATGAGGCAACCGCATGTGCTG
CAACTGATCATGGATTCGCTTCGCTATTGGGTTCTGGAAATGCACGTCGATGGCTTCCGCTTCGATCTCGCATCCGCGCT
CGCACGCGAACTCCATGAAGTGCAAATGCTGAATGCATTTTTCAACATTATCCAGCAGGATCCTGTTACAAATCAGGTCA
AGCTGATCGCGGAACCCTGGGACCTGGGGGAAGGCGGTTACCAAGTGGGGAAATTCCCGTCAGGCTGGTCTGAATGGAAC
GGCAAGTACCGGGACTGTATCCGCAATTTCTGGCGCACCCAGGAACCTACTTTAAGTGAATTCGCTTATCGCTTTACCGG
AAGCTCAGACCTTTACGAGGGGAATTCGCGTCATCCTTTTGCAAGCATCAATTTTGTCACCTCGCACGACGGTTTCACAT
TGCGGGATCTTGTCTCCTATAACGAAAAACACAACCTGGCCAATGGCGAGGACAACAGGGATGGCACTGACGACAACCGT
TCCTGGAACTGCGGTGTCGAAGGGCCTACGGATGACATGGAAGTGCTTACGCTTCGCGCCCGGCAGCAGCGCAATTTTCT
GGCTACCCTGGTATTGTCTCAGGGCGTGCCCATGTTGCTGGCAGGGGATGAATTGGGTCGCACCCAGCAGGGAAACAATA
ATGCGTACTGCCAGGACAACGAAATTTCCTGGGTGGATTGGGGGAAGGTGGATACCGGTTTACAGGAATTCACCAGACGC
CTTGCTCGCTTCCGCCGTGATCACCCGGTCTTCCGCCGACGCCGGTGGTTTCAGGGGCAACCCATTCACGGTGGGGGCCA
GGACGATATTGCGTGGTTCAATTACATGGGAGAACAGGCGAGCGAGGAATTATGGGGGAATGGGGGAATTCAAAGCCTGG
GAATTTTTCTCAACGGAGACAGTTTCCCGAATCCCAATGCTCGAGGGGAGCCGGTAAAGGATGACAGCTTCTACCTGATT
TTCAACGCACATTTCGAGCCGATCGATTTCGTCCTGCCCCCCAACCACTGGGGGCTACGCTGGTTGAGAATACTCGACAC
CAACGAGGGCTGGGTAGAGAATGCAGCGGATCAGCCCGGGCTCGAAGCCGGATCCGCTTTGTCAGTCGCCGCCCGGTCAC
TGGTGCTTTTGCAGCGGCAGGCATGA

Upstream 100 bases:

>100_bases
TAAGCGAATTTTCATTTGCCATCTCTCTGTTGGCTGGTCCGAAAATAATATAGCACTGTAAGTTATAGCAGCATTCCGCA
ACTTTGACGCAGGTACCCGA

Downstream 100 bases:

>100_bases
AACCCTCTCATCAGGATGAGCCTCTGCAAATATCCCCGCTTCATCCCGTTGTCTGCTCGAAAGGGGATAGCAACGCATAA
GGAAACGTCCCGAAAAGGTG

Product: glycogen debranching protein GlgX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 701; Mature: 701

Protein sequence:

>701_residues
MKIWPGTPYPLGATYDGAGTNFSLFSEAAERVELCLFDEAGRETRVNLPEVTGYCWHGYLPGVEPGQRYGFRVHGPWSPE
QGNRCNSAKLLLDPYAKGIDGDITWDEAVFPYLFDDRDARNDKDSAPFMPRSIVHQPFFDWSGDRQLQRPWHETVIYELH
VKGFTAQHPDIPPELRGTYAGLAHSASIDYLKQLGVTAVELLPVHYFVQDKHLLDQGLRNYWGYNSIAYFAPHSAYAADK
RPGAASAEFKQMVKALHQADIEVILDVVYNHTAEGNDLGPVLCLKGIDNAYYYQLMEDRRYYRDYTGTGNCLNMRQPHVL
QLIMDSLRYWVLEMHVDGFRFDLASALARELHEVQMLNAFFNIIQQDPVTNQVKLIAEPWDLGEGGYQVGKFPSGWSEWN
GKYRDCIRNFWRTQEPTLSEFAYRFTGSSDLYEGNSRHPFASINFVTSHDGFTLRDLVSYNEKHNLANGEDNRDGTDDNR
SWNCGVEGPTDDMEVLTLRARQQRNFLATLVLSQGVPMLLAGDELGRTQQGNNNAYCQDNEISWVDWGKVDTGLQEFTRR
LARFRRDHPVFRRRRWFQGQPIHGGGQDDIAWFNYMGEQASEELWGNGGIQSLGIFLNGDSFPNPNARGEPVKDDSFYLI
FNAHFEPIDFVLPPNHWGLRWLRILDTNEGWVENAADQPGLEAGSALSVAARSLVLLQRQA

Sequences:

>Translated_701_residues
MKIWPGTPYPLGATYDGAGTNFSLFSEAAERVELCLFDEAGRETRVNLPEVTGYCWHGYLPGVEPGQRYGFRVHGPWSPE
QGNRCNSAKLLLDPYAKGIDGDITWDEAVFPYLFDDRDARNDKDSAPFMPRSIVHQPFFDWSGDRQLQRPWHETVIYELH
VKGFTAQHPDIPPELRGTYAGLAHSASIDYLKQLGVTAVELLPVHYFVQDKHLLDQGLRNYWGYNSIAYFAPHSAYAADK
RPGAASAEFKQMVKALHQADIEVILDVVYNHTAEGNDLGPVLCLKGIDNAYYYQLMEDRRYYRDYTGTGNCLNMRQPHVL
QLIMDSLRYWVLEMHVDGFRFDLASALARELHEVQMLNAFFNIIQQDPVTNQVKLIAEPWDLGEGGYQVGKFPSGWSEWN
GKYRDCIRNFWRTQEPTLSEFAYRFTGSSDLYEGNSRHPFASINFVTSHDGFTLRDLVSYNEKHNLANGEDNRDGTDDNR
SWNCGVEGPTDDMEVLTLRARQQRNFLATLVLSQGVPMLLAGDELGRTQQGNNNAYCQDNEISWVDWGKVDTGLQEFTRR
LARFRRDHPVFRRRRWFQGQPIHGGGQDDIAWFNYMGEQASEELWGNGGIQSLGIFLNGDSFPNPNARGEPVKDDSFYLI
FNAHFEPIDFVLPPNHWGLRWLRILDTNEGWVENAADQPGLEAGSALSVAARSLVLLQRQA
>Mature_701_residues
MKIWPGTPYPLGATYDGAGTNFSLFSEAAERVELCLFDEAGRETRVNLPEVTGYCWHGYLPGVEPGQRYGFRVHGPWSPE
QGNRCNSAKLLLDPYAKGIDGDITWDEAVFPYLFDDRDARNDKDSAPFMPRSIVHQPFFDWSGDRQLQRPWHETVIYELH
VKGFTAQHPDIPPELRGTYAGLAHSASIDYLKQLGVTAVELLPVHYFVQDKHLLDQGLRNYWGYNSIAYFAPHSAYAADK
RPGAASAEFKQMVKALHQADIEVILDVVYNHTAEGNDLGPVLCLKGIDNAYYYQLMEDRRYYRDYTGTGNCLNMRQPHVL
QLIMDSLRYWVLEMHVDGFRFDLASALARELHEVQMLNAFFNIIQQDPVTNQVKLIAEPWDLGEGGYQVGKFPSGWSEWN
GKYRDCIRNFWRTQEPTLSEFAYRFTGSSDLYEGNSRHPFASINFVTSHDGFTLRDLVSYNEKHNLANGEDNRDGTDDNR
SWNCGVEGPTDDMEVLTLRARQQRNFLATLVLSQGVPMLLAGDELGRTQQGNNNAYCQDNEISWVDWGKVDTGLQEFTRR
LARFRRDHPVFRRRRWFQGQPIHGGGQDDIAWFNYMGEQASEELWGNGGIQSLGIFLNGDSFPNPNARGEPVKDDSFYLI
FNAHFEPIDFVLPPNHWGLRWLRILDTNEGWVENAADQPGLEAGSALSVAARSLVLLQRQA

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=652, Percent_Identity=48.3128834355828, Blast_Score=594, Evalue=1e-171,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 79924; Mature: 79924

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIWPGTPYPLGATYDGAGTNFSLFSEAAERVELCLFDEAGRETRVNLPEVTGYCWHGYL
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCEEEECCC
PGVEPGQRYGFRVHGPWSPEQGNRCNSAKLLLDPYAKGIDGDITWDEAVFPYLFDDRDAR
CCCCCCCCCCEEEECCCCCCCCCCCCCCEEEECHHHCCCCCCCCCCCCCCCEEECCCCCC
NDKDSAPFMPRSIVHQPFFDWSGDRQLQRPWHETVIYELHVKGFTAQHPDIPPELRGTYA
CCCCCCCCCCHHHHCCCCCCCCCCCHHHCCHHHEEEEEEEECCEECCCCCCCHHHHHHHH
GLAHSASIDYLKQLGVTAVELLPVHYFVQDKHLLDQGLRNYWGYNSIAYFAPHSAYAADK
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCC
RPGAASAEFKQMVKALHQADIEVILDVVYNHTAEGNDLGPVLCLKGIDNAYYYQLMEDRR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHH
YYRDYTGTGNCLNMRQPHVLQLIMDSLRYWVLEMHVDGFRFDLASALARELHEVQMLNAF
HHHCCCCCCCEECCCCHHHHHHHHHHHHHEEHEEECCCHHHHHHHHHHHHHHHHHHHHHH
FNIIQQDPVTNQVKLIAEPWDLGEGGYQVGKFPSGWSEWNGKYRDCIRNFWRTQEPTLSE
HHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHCCCCCCHHH
FAYRFTGSSDLYEGNSRHPFASINFVTSHDGFTLRDLVSYNEKHNLANGEDNRDGTDDNR
HHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHCHHCCCCCCCCCCCCCCCCC
SWNCGVEGPTDDMEVLTLRARQQRNFLATLVLSQGVPMLLAGDELGRTQQGNNNAYCQDN
CEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHCCCCCCCCCCCEECC
EISWVDWGKVDTGLQEFTRRLARFRRDHPVFRRRRWFQGQPIHGGGQDDIAWFNYMGEQA
CCCEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
SEELWGNGGIQSLGIFLNGDSFPNPNARGEPVKDDSFYLIFNAHFEPIDFVLPPNHWGLR
HHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCEE
WLRILDTNEGWVENAADQPGLEAGSALSVAARSLVLLQRQA
EEEEEECCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKIWPGTPYPLGATYDGAGTNFSLFSEAAERVELCLFDEAGRETRVNLPEVTGYCWHGYL
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCEEEECCC
PGVEPGQRYGFRVHGPWSPEQGNRCNSAKLLLDPYAKGIDGDITWDEAVFPYLFDDRDAR
CCCCCCCCCCEEEECCCCCCCCCCCCCCEEEECHHHCCCCCCCCCCCCCCCEEECCCCCC
NDKDSAPFMPRSIVHQPFFDWSGDRQLQRPWHETVIYELHVKGFTAQHPDIPPELRGTYA
CCCCCCCCCCHHHHCCCCCCCCCCCHHHCCHHHEEEEEEEECCEECCCCCCCHHHHHHHH
GLAHSASIDYLKQLGVTAVELLPVHYFVQDKHLLDQGLRNYWGYNSIAYFAPHSAYAADK
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCC
RPGAASAEFKQMVKALHQADIEVILDVVYNHTAEGNDLGPVLCLKGIDNAYYYQLMEDRR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHH
YYRDYTGTGNCLNMRQPHVLQLIMDSLRYWVLEMHVDGFRFDLASALARELHEVQMLNAF
HHHCCCCCCCEECCCCHHHHHHHHHHHHHEEHEEECCCHHHHHHHHHHHHHHHHHHHHHH
FNIIQQDPVTNQVKLIAEPWDLGEGGYQVGKFPSGWSEWNGKYRDCIRNFWRTQEPTLSE
HHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHCCCCCCHHH
FAYRFTGSSDLYEGNSRHPFASINFVTSHDGFTLRDLVSYNEKHNLANGEDNRDGTDDNR
HHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHCHHCCCCCCCCCCCCCCCCC
SWNCGVEGPTDDMEVLTLRARQQRNFLATLVLSQGVPMLLAGDELGRTQQGNNNAYCQDN
CEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHCCCCCCCCCCCEECC
EISWVDWGKVDTGLQEFTRRLARFRRDHPVFRRRRWFQGQPIHGGGQDDIAWFNYMGEQA
CCCEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
SEELWGNGGIQSLGIFLNGDSFPNPNARGEPVKDDSFYLIFNAHFEPIDFVLPPNHWGLR
HHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCEE
WLRILDTNEGWVENAADQPGLEAGSALSVAARSLVLLQRQA
EEEEEECCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]