Click here to switch to the map view.

The map label for this gene is flgJ [H]

Identifier: 82702458

GI number: 82702458

Start: 1517889

End: 1518941

Strand: Direct

Name: flgJ [H]

Synonym: Nmul_A1329

Alternate gene names: 82702458

Gene position: 1517889-1518941 (Clockwise)

Preceding gene: 82702457

Following gene: 82702459

Centisome position: 47.67

GC content: 57.45

Gene sequence:

>1053_bases
ATGGTTGCTCCGGTTGATCTATCCACGCGCTTTGCCCTTGACGTCCAGGCGGTAAACGGGTTACGCGTGGAATCAAAAAA
AACCGGCGAAGCGGGTCTTGAAGCCGCAGCCAGGCAGTTCGAAGCGCTCTTTCTGAACATGCTGCTGAAAAGCATGCGCG
ATGCCACTCCTAAAGACGATCTGATGGAGAGCGAACAGAGCCGACTGTATCTCTCGATGCTGGATCAACAGTTTTCCCAG
ACCATGGCTGCGCGCGGCATTGGCCTTGCCGAGATCATGGTGCGTCAATTGCGCCATGCTGCGCCGCCTGATCCGACAGA
GAAAGCGGGGATTCCGCAAAACCCCAATAGCCATGCCTTGGATCAATCAATGCCACGCTCTTCAGCGGAGGCAGGGAAGC
ATCCGGGTTTGCGGGGTTATGCACCGACAGTAGAAATGCAGGAACGACTGCAGGAAATAGAGGGACAAAAGGAACTCGCA
AAACCCGGACAGATCGGGCAGCGAGGTCAGTCAGGGTGGTCTGGAGCGGGACAGTCAGCTCCATCCAGGTTGGAACAGGA
GGGGCCACAGGCGAGCGAAATACCCGCGCATGTGTCCGAGTTCAAGGCAAGGGTAGGAGCATATGCCCAGGAAGTGAGCC
AGGCAACGGGTATTCCGGTCCAGTTCATGCTCGGGCAGGCAGCACTGGAAAGCGGATGGGGGAAACGCGAGTTGCGTGCT
ACCGATGGAACCCACAGCCACAACCTCTTTGGCATAAAAGCGGGGACCGGCTGGAAGGGGCCCGTGGTGGAAGCGGTCAC
GACAGAATACATCAATGGCATTGCACATAAGAGGGTGGAGAAATTTCGCGCTTATCCATCCTACGCCGAAGCTTTCCGCG
ACTACGCCACCCTTTTGAGCACCAATCCACGCTACACGGAAGCACTCAGGCAAGCAGTGCAGAAGTTCGACGCCGAAGGA
TTCGCCCATGCCCTGCAGCGGGCGGGCTATGCTACTGACCCGGCCTATGGAGACAAGTTAGGCCGGATCATCAGGACCAC
TCCCTGGTCCTGA

Upstream 100 bases:

>100_bases
AGGCATTGAACGCAATCGGCGCGACGCCGCAGGATCTACTGGCAATTTTACAGGCGATGAAGTCCGCCGGTGCGCTGCGG
GCTGAGCTGGAAATCATCTG

Downstream 100 bases:

>100_bases
GCCTCCATAAGGGGTAAGAACCGGCGGCAAATCTTTCGGAGGATTTCTCCGTCCCCCTAAATATTTTCAACCCGATGCCG
TCAATATATGCACGACGACA

Product: mannosyl-glycoprotein endo-beta-N-acetylglucosamidase

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MVAPVDLSTRFALDVQAVNGLRVESKKTGEAGLEAAARQFEALFLNMLLKSMRDATPKDDLMESEQSRLYLSMLDQQFSQ
TMAARGIGLAEIMVRQLRHAAPPDPTEKAGIPQNPNSHALDQSMPRSSAEAGKHPGLRGYAPTVEMQERLQEIEGQKELA
KPGQIGQRGQSGWSGAGQSAPSRLEQEGPQASEIPAHVSEFKARVGAYAQEVSQATGIPVQFMLGQAALESGWGKRELRA
TDGTHSHNLFGIKAGTGWKGPVVEAVTTEYINGIAHKRVEKFRAYPSYAEAFRDYATLLSTNPRYTEALRQAVQKFDAEG
FAHALQRAGYATDPAYGDKLGRIIRTTPWS

Sequences:

>Translated_350_residues
MVAPVDLSTRFALDVQAVNGLRVESKKTGEAGLEAAARQFEALFLNMLLKSMRDATPKDDLMESEQSRLYLSMLDQQFSQ
TMAARGIGLAEIMVRQLRHAAPPDPTEKAGIPQNPNSHALDQSMPRSSAEAGKHPGLRGYAPTVEMQERLQEIEGQKELA
KPGQIGQRGQSGWSGAGQSAPSRLEQEGPQASEIPAHVSEFKARVGAYAQEVSQATGIPVQFMLGQAALESGWGKRELRA
TDGTHSHNLFGIKAGTGWKGPVVEAVTTEYINGIAHKRVEKFRAYPSYAEAFRDYATLLSTNPRYTEALRQAVQKFDAEG
FAHALQRAGYATDPAYGDKLGRIIRTTPWS
>Mature_350_residues
MVAPVDLSTRFALDVQAVNGLRVESKKTGEAGLEAAARQFEALFLNMLLKSMRDATPKDDLMESEQSRLYLSMLDQQFSQ
TMAARGIGLAEIMVRQLRHAAPPDPTEKAGIPQNPNSHALDQSMPRSSAEAGKHPGLRGYAPTVEMQERLQEIEGQKELA
KPGQIGQRGQSGWSGAGQSAPSRLEQEGPQASEIPAHVSEFKARVGAYAQEVSQATGIPVQFMLGQAALESGWGKRELRA
TDGTHSHNLFGIKAGTGWKGPVVEAVTTEYINGIAHKRVEKFRAYPSYAEAFRDYATLLSTNPRYTEALRQAVQKFDAEG
FAHALQRAGYATDPAYGDKLGRIIRTTPWS

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=346, Percent_Identity=38.150289017341, Blast_Score=207, Evalue=6e-55,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR000423
- InterPro:   IPR019301
- InterPro:   IPR013338
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.-

Molecular weight: Translated: 38179; Mature: 38179

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAPVDLSTRFALDVQAVNGLRVESKKTGEAGLEAAARQFEALFLNMLLKSMRDATPKDD
CCCCCCCCCHHEEEHHHHCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LMESEQSRLYLSMLDQQFSQTMAARGIGLAEIMVRQLRHAAPPDPTEKAGIPQNPNSHAL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHH
DQSMPRSSAEAGKHPGLRGYAPTVEMQERLQEIEGQKELAKPGQIGQRGQSGWSGAGQSA
HHHCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHCHHHHCCCCCCCCCCCCCCCCCCCCC
PSRLEQEGPQASEIPAHVSEFKARVGAYAQEVSQATGIPVQFMLGQAALESGWGKRELRA
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHCC
TDGTHSHNLFGIKAGTGWKGPVVEAVTTEYINGIAHKRVEKFRAYPSYAEAFRDYATLLS
CCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
TNPRYTEALRQAVQKFDAEGFAHALQRAGYATDPAYGDKLGRIIRTTPWS
CCCHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVAPVDLSTRFALDVQAVNGLRVESKKTGEAGLEAAARQFEALFLNMLLKSMRDATPKDD
CCCCCCCCCHHEEEHHHHCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LMESEQSRLYLSMLDQQFSQTMAARGIGLAEIMVRQLRHAAPPDPTEKAGIPQNPNSHAL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHH
DQSMPRSSAEAGKHPGLRGYAPTVEMQERLQEIEGQKELAKPGQIGQRGQSGWSGAGQSA
HHHCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHCHHHHCCCCCCCCCCCCCCCCCCCCC
PSRLEQEGPQASEIPAHVSEFKARVGAYAQEVSQATGIPVQFMLGQAALESGWGKRELRA
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHCC
TDGTHSHNLFGIKAGTGWKGPVVEAVTTEYINGIAHKRVEKFRAYPSYAEAFRDYATLLS
CCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
TNPRYTEALRQAVQKFDAEGFAHALQRAGYATDPAYGDKLGRIIRTTPWS
CCCHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]