Click here to switch to the map view.

The map label for this gene is eno

Identifier: 82702357

GI number: 82702357

Start: 1411940

End: 1413223

Strand: Direct

Name: eno

Synonym: Nmul_A1228

Alternate gene names: 82702357

Gene position: 1411940-1413223 (Clockwise)

Preceding gene: 82702356

Following gene: 82702358

Centisome position: 44.34

GC content: 54.44

Gene sequence:

>1284_bases
ATGAGTGCAATTGTAGATGTCATCGCCCGCGAAATTCTTGACTCGCGCGGAAACCCAACCGTGGAAGCGGATGTTTTGCT
TGAATCCGGAGTATTGGGCCGGGCGGCTGTTCCTTCCGGGGCATCGGTTGGCACCCGGGAGGCTGTCGAATTGCGCGATG
AGGACGCTCAGCGCTATTTCGGTAAAGGCGTACTGAAGGCGGTGGAAAATGTGAACACCGAAATTTCAGAAGCAATCATG
GGACTGGATGCAATGGACCAGGCATTTATCGACCGCACACTGATCGATCTCGATGGCAGCGGCAATAAATCAAGGCTCGG
CGCCAACGCGATTCTTTCCGTATCGCTTGCGGTGGCCAAGGCGGCGGCGGAAGAATGCGGACTCCCGCTGTACCGGTATG
TCGGGGGAGCGGGATCGATGTCCATGCCTGTTCCCATGATGAACGTCATCAATGGCGGGGCCCATGCCAATAACAATATT
GACATGCAGGAGTTCGTAATCATTCCTCTGGGCGCCCAGAGTTTTCGCGAAGCTCTGCGTTGCGGCGCGGAAGTATTTCA
TACCTTGAAAGGCTTGCTCGACAAGAGAGGCATGCATACCGCGGTGGGTGACGAAGGCGGCTTTGCACCCGATCTGCCCA
ATAATGAGGCAGCATTGCAACTGATCGTGGAAGCGATAGAGAACGCGGGCTATCTTCCGGGACCGGATGTCGCCATTGGG
CTGGATTGCGCTGCTTCGGAGTTTTACCGTGACGGCAAATACCATCTGGAGTCGGATGGCCTGGCGCTCGATTCGACCCA
GTTTGCCGATTATCTTGCCACCTGGATCGACAAATATCCCATTATCAGCATTGAGGACGGGATGAGCGAACATGACTGGG
ATGGCTGGAAACTGCTCACCAGCAGACTTGGGAAATCCGTGCAACTGGTGGGCGACGATATTTTTGTTACAAACGCGAGC
ATTCTGAAAGAAGGGGTCTCCCAGGGCATCGCAAATTCCATATTGATCAAGCTCAATCAGATCGGGACGCTTACCGAAGC
GCTTTATGCGATTGAAAGTGCAAAGCGTGCCGGCTATACAGCAGTGGTCTCGCACCGTTCGGGTGAAACCGAAGATACCA
CGATCGCGGACATCGCAGTGGCAAGCAATGCATTGCAGATCAAGACCGGTTCGCTTTCCCGTTCCGACCGGCTTGCAAAA
TACAATCAACTCCTGCGCATCGAGGAAGATCTGGGCGACACCGCCAGTTATCCCGGTCGTGGTGCCTTCTACCAGTTGAA
ATAA

Upstream 100 bases:

>100_bases
CGTTTCAGCCTCTAGTCCTCAGGCCGATTCTCCGGTAGTCCTCGGAGAGTGGCAGAATCGTTTTCAATAACTTGCAGATA
AATGATCAGGGAAAAATAGC

Downstream 100 bases:

>100_bases
GCCTTTTCAAATCCGAGCGGACAGGTCAGGAATTCCTCATGGAAGGAATCCTGGGAGAAGCCGAAGTGAAAGTATTGACT
CTTATACTCGTGGCATTAAT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MSAIVDVIAREILDSRGNPTVEADVLLESGVLGRAAVPSGASVGTREAVELRDEDAQRYFGKGVLKAVENVNTEISEAIM
GLDAMDQAFIDRTLIDLDGSGNKSRLGANAILSVSLAVAKAAAEECGLPLYRYVGGAGSMSMPVPMMNVINGGAHANNNI
DMQEFVIIPLGAQSFREALRCGAEVFHTLKGLLDKRGMHTAVGDEGGFAPDLPNNEAALQLIVEAIENAGYLPGPDVAIG
LDCAASEFYRDGKYHLESDGLALDSTQFADYLATWIDKYPIISIEDGMSEHDWDGWKLLTSRLGKSVQLVGDDIFVTNAS
ILKEGVSQGIANSILIKLNQIGTLTEALYAIESAKRAGYTAVVSHRSGETEDTTIADIAVASNALQIKTGSLSRSDRLAK
YNQLLRIEEDLGDTASYPGRGAFYQLK

Sequences:

>Translated_427_residues
MSAIVDVIAREILDSRGNPTVEADVLLESGVLGRAAVPSGASVGTREAVELRDEDAQRYFGKGVLKAVENVNTEISEAIM
GLDAMDQAFIDRTLIDLDGSGNKSRLGANAILSVSLAVAKAAAEECGLPLYRYVGGAGSMSMPVPMMNVINGGAHANNNI
DMQEFVIIPLGAQSFREALRCGAEVFHTLKGLLDKRGMHTAVGDEGGFAPDLPNNEAALQLIVEAIENAGYLPGPDVAIG
LDCAASEFYRDGKYHLESDGLALDSTQFADYLATWIDKYPIISIEDGMSEHDWDGWKLLTSRLGKSVQLVGDDIFVTNAS
ILKEGVSQGIANSILIKLNQIGTLTEALYAIESAKRAGYTAVVSHRSGETEDTTIADIAVASNALQIKTGSLSRSDRLAK
YNQLLRIEEDLGDTASYPGRGAFYQLK
>Mature_426_residues
SAIVDVIAREILDSRGNPTVEADVLLESGVLGRAAVPSGASVGTREAVELRDEDAQRYFGKGVLKAVENVNTEISEAIMG
LDAMDQAFIDRTLIDLDGSGNKSRLGANAILSVSLAVAKAAAEECGLPLYRYVGGAGSMSMPVPMMNVINGGAHANNNID
MQEFVIIPLGAQSFREALRCGAEVFHTLKGLLDKRGMHTAVGDEGGFAPDLPNNEAALQLIVEAIENAGYLPGPDVAIGL
DCAASEFYRDGKYHLESDGLALDSTQFADYLATWIDKYPIISIEDGMSEHDWDGWKLLTSRLGKSVQLVGDDIFVTNASI
LKEGVSQGIANSILIKLNQIGTLTEALYAIESAKRAGYTAVVSHRSGETEDTTIADIAVASNALQIKTGSLSRSDRLAKY
NQLLRIEEDLGDTASYPGRGAFYQLK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=430, Percent_Identity=52.093023255814, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI301897477, Length=431, Percent_Identity=52.6682134570766, Blast_Score=415, Evalue=1e-116,
Organism=Homo sapiens, GI301897469, Length=431, Percent_Identity=52.6682134570766, Blast_Score=415, Evalue=1e-116,
Organism=Homo sapiens, GI4503571, Length=430, Percent_Identity=51.1627906976744, Blast_Score=413, Evalue=1e-115,
Organism=Homo sapiens, GI301897479, Length=429, Percent_Identity=48.018648018648, Blast_Score=356, Evalue=3e-98,
Organism=Homo sapiens, GI169201331, Length=336, Percent_Identity=26.1904761904762, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI169201757, Length=336, Percent_Identity=26.1904761904762, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI239744207, Length=336, Percent_Identity=26.1904761904762, Blast_Score=102, Evalue=7e-22,
Organism=Escherichia coli, GI1789141, Length=425, Percent_Identity=62.3529411764706, Blast_Score=526, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI71995829, Length=430, Percent_Identity=51.8604651162791, Blast_Score=412, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17536383, Length=430, Percent_Identity=51.8604651162791, Blast_Score=412, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI32563855, Length=190, Percent_Identity=46.8421052631579, Blast_Score=174, Evalue=6e-44,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=50.1154734411085, Blast_Score=384, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=50.1154734411085, Blast_Score=384, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=49.8845265588915, Blast_Score=383, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6321693, Length=432, Percent_Identity=48.8425925925926, Blast_Score=382, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6321968, Length=432, Percent_Identity=49.3055555555556, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580918, Length=430, Percent_Identity=50.4651162790698, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580916, Length=430, Percent_Identity=50.4651162790698, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580920, Length=430, Percent_Identity=50.4651162790698, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580914, Length=430, Percent_Identity=50.4651162790698, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI281360527, Length=427, Percent_Identity=50.5854800936768, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI17137654, Length=427, Percent_Identity=50.5854800936768, Blast_Score=379, Evalue=1e-105,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_NITMU (Q2Y9P0)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_411923.1
- HSSP:   P0A6P9
- ProteinModelPortal:   Q2Y9P0
- SMR:   Q2Y9P0
- STRING:   Q2Y9P0
- GeneID:   3785567
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A1228
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- PhylomeDB:   Q2Y9P0
- ProtClustDB:   PRK00077
- BioCyc:   NMUL323848:NMUL_A1228-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 45548; Mature: 45417

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 337-337 BINDING 155-155 BINDING 164-164 BINDING 285-285 BINDING 312-312 BINDING 337-337 BINDING 388-388

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIVDVIAREILDSRGNPTVEADVLLESGVLGRAAVPSGASVGTREAVELRDEDAQRYF
CHHHHHHHHHHHHHCCCCCCEEEHHEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
GKGVLKAVENVNTEISEAIMGLDAMDQAFIDRTLIDLDGSGNKSRLGANAILSVSLAVAK
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHH
AAAEECGLPLYRYVGGAGSMSMPVPMMNVINGGAHANNNIDMQEFVIIPLGAQSFREALR
HHHHHHCCCHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHEEEEECCHHHHHHHHH
CGAEVFHTLKGLLDKRGMHTAVGDEGGFAPDLPNNEAALQLIVEAIENAGYLPGPDVAIG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
LDCAASEFYRDGKYHLESDGLALDSTQFADYLATWIDKYPIISIEDGMSEHDWDGWKLLT
CHHHHHHHHCCCCEEECCCCCEECHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHH
SRLGKSVQLVGDDIFVTNASILKEGVSQGIANSILIKLNQIGTLTEALYAIESAKRAGYT
HHCCCEEEEECCEEEEECHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHCCCE
AVVSHRSGETEDTTIADIAVASNALQIKTGSLSRSDRLAKYNQLLRIEEDLGDTASYPGR
EEEECCCCCCCCCCHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
GAFYQLK
CCEEECC
>Mature Secondary Structure 
SAIVDVIAREILDSRGNPTVEADVLLESGVLGRAAVPSGASVGTREAVELRDEDAQRYF
HHHHHHHHHHHHHCCCCCCEEEHHEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
GKGVLKAVENVNTEISEAIMGLDAMDQAFIDRTLIDLDGSGNKSRLGANAILSVSLAVAK
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHH
AAAEECGLPLYRYVGGAGSMSMPVPMMNVINGGAHANNNIDMQEFVIIPLGAQSFREALR
HHHHHHCCCHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHEEEEECCHHHHHHHHH
CGAEVFHTLKGLLDKRGMHTAVGDEGGFAPDLPNNEAALQLIVEAIENAGYLPGPDVAIG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
LDCAASEFYRDGKYHLESDGLALDSTQFADYLATWIDKYPIISIEDGMSEHDWDGWKLLT
CHHHHHHHHCCCCEEECCCCCEECHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHH
SRLGKSVQLVGDDIFVTNASILKEGVSQGIANSILIKLNQIGTLTEALYAIESAKRAGYT
HHCCCEEEEECCEEEEECHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHCCCE
AVVSHRSGETEDTTIADIAVASNALQIKTGSLSRSDRLAKYNQLLRIEEDLGDTASYPGR
EEEECCCCCCCCCCHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
GAFYQLK
CCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA