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The map label for this gene is yfhA [H]

Identifier: 82702290

GI number: 82702290

Start: 1338898

End: 1340259

Strand: Direct

Name: yfhA [H]

Synonym: Nmul_A1161

Alternate gene names: 82702290

Gene position: 1338898-1340259 (Clockwise)

Preceding gene: 82702289

Following gene: 82702291

Centisome position: 42.05

GC content: 55.29

Gene sequence:

>1362_bases
ATGTCCGATGCAAAACCCAGAATTCTGATCGTTGACGACGACACCGATCTGCTTGAGCTGCTGACCATGCGCCTTACAGC
GGCGGGATACAAAGTGGACGCCGTTCCAAGCGCAGAGGCGGCGCTGAACTATCTGGACGTGTCCCGTCCGCAACTGGTCA
TCAGCGATATGCAGATGAGCGGCATGGACGGCATGGCATTATTTGAACAAATCCATCGCTCCTTGCCGACCTTGCCGGTG
ATCATACTCACGGCGCACGGAACGATCCCCGATGCCGTCACGGCTGTCCAGCGAGGGGTATTCGGCTATCTTGCCAAACC
TTTTGACAGCAAGACGTTGCTGGCAAATATCAGTCAGGCGCTCAAGTTCGTTCCGGGCGCCGTATCGCAGAAGGAACTGT
CGCAGGCACCGTGGCGCAAGAATATCATCACTCAGAGCGCCGTCATGGAGGATCTTCTTACCCGGGCGGGACTGGTTGCG
GAAGGTGACGCCAGCGTGCTGATCTATGGAGAAAGCGGAGTGGGCAAGGAGCTGTTCGCGCATGCCATACATGATGCATC
CAAACGATCCGATCATCCGTTCGTGGCGATCAATTGCGCCGCCATTCCGGAACAGTTGCTGGAATCCGAGCTGTTCGGGC
ATGTCAAAGGGGCATTTACCGGGGCTGTCAGGGATCACAAGGGTTTATTCCAGCTGGCCGAGGGGGGCACTCTCTTTCTC
GACGAAATCGGCGATATGCCGGTTTTGCTTCAGGTCAAGCTGTTGCGTGTTCTCCAGGAAAGGCAGGTTCGACCGGTTGG
GTCGGCACAATCGATTCCGGTGGATGTGCGTATTATTTCGGCTACTCACCGCGATCTGAAAACGGAAATCGCTGCTGGAA
CATTTCGGGAAGACCTGTATTACAGACTGGACGTGGTGTCCCTTACCATCCCGGTTCTGGCGCAAAGGCGCGAAGATATT
CCTTTACTGGTAAATTACTTCCTCAGCACATTTTCAGAGAAATACCAGAAAAATATCAATGGCTTCTCTCCCGAGGCCAT
GGAAACACTGGTTGCCGCGTCCTGGCCCGGCAATGTGCGCCAACTCATGAACGTCGTCGAGAGATGCGTTGCATTGAGTA
CTGCGCCCCTTATTTCGCCCGTGCTGGTCTACGATGCGATGCACCGGGAAGAAGAGCAACTCGTGTCTTTCGAGGATGCC
CGCAGAAGTTTTGAACGGGATTATCTGGTGCGCGTACTTAAAATCACCGGAGGAAACGTCACCCAGGCAGCCCGCCTCGC
CAAGCGCAACCGCACGGAGTTCTACAAGCTGCTGCAGCGATACCAGCTCGATCCATCTGTCTTCAAGCAGGCGCAGGTTT
GA

Upstream 100 bases:

>100_bases
AGCAGAAGCGTGTAGAAACCCTGCAGAGTCAGATCGATGCCATAAAAAGCATGGAAAAGAATCTGCTTAAAGAATTGGAA
TTGTAAAGAAACCTAAAATC

Downstream 100 bases:

>100_bases
GAAAAGGAAGGACGGGGCTGAGCGGCACAGGTTCTGCTAGAGTATCCTCCTTTTTTCCTTCCTGGCCGGATCAGGTCTCG
CCGGCGATGGGTCTGCGCTC

Product: two component sigma-54 specific Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MSDAKPRILIVDDDTDLLELLTMRLTAAGYKVDAVPSAEAALNYLDVSRPQLVISDMQMSGMDGMALFEQIHRSLPTLPV
IILTAHGTIPDAVTAVQRGVFGYLAKPFDSKTLLANISQALKFVPGAVSQKELSQAPWRKNIITQSAVMEDLLTRAGLVA
EGDASVLIYGESGVGKELFAHAIHDASKRSDHPFVAINCAAIPEQLLESELFGHVKGAFTGAVRDHKGLFQLAEGGTLFL
DEIGDMPVLLQVKLLRVLQERQVRPVGSAQSIPVDVRIISATHRDLKTEIAAGTFREDLYYRLDVVSLTIPVLAQRREDI
PLLVNYFLSTFSEKYQKNINGFSPEAMETLVAASWPGNVRQLMNVVERCVALSTAPLISPVLVYDAMHREEEQLVSFEDA
RRSFERDYLVRVLKITGGNVTQAARLAKRNRTEFYKLLQRYQLDPSVFKQAQV

Sequences:

>Translated_453_residues
MSDAKPRILIVDDDTDLLELLTMRLTAAGYKVDAVPSAEAALNYLDVSRPQLVISDMQMSGMDGMALFEQIHRSLPTLPV
IILTAHGTIPDAVTAVQRGVFGYLAKPFDSKTLLANISQALKFVPGAVSQKELSQAPWRKNIITQSAVMEDLLTRAGLVA
EGDASVLIYGESGVGKELFAHAIHDASKRSDHPFVAINCAAIPEQLLESELFGHVKGAFTGAVRDHKGLFQLAEGGTLFL
DEIGDMPVLLQVKLLRVLQERQVRPVGSAQSIPVDVRIISATHRDLKTEIAAGTFREDLYYRLDVVSLTIPVLAQRREDI
PLLVNYFLSTFSEKYQKNINGFSPEAMETLVAASWPGNVRQLMNVVERCVALSTAPLISPVLVYDAMHREEEQLVSFEDA
RRSFERDYLVRVLKITGGNVTQAARLAKRNRTEFYKLLQRYQLDPSVFKQAQV
>Mature_452_residues
SDAKPRILIVDDDTDLLELLTMRLTAAGYKVDAVPSAEAALNYLDVSRPQLVISDMQMSGMDGMALFEQIHRSLPTLPVI
ILTAHGTIPDAVTAVQRGVFGYLAKPFDSKTLLANISQALKFVPGAVSQKELSQAPWRKNIITQSAVMEDLLTRAGLVAE
GDASVLIYGESGVGKELFAHAIHDASKRSDHPFVAINCAAIPEQLLESELFGHVKGAFTGAVRDHKGLFQLAEGGTLFLD
EIGDMPVLLQVKLLRVLQERQVRPVGSAQSIPVDVRIISATHRDLKTEIAAGTFREDLYYRLDVVSLTIPVLAQRREDIP
LLVNYFLSTFSEKYQKNINGFSPEAMETLVAASWPGNVRQLMNVVERCVALSTAPLISPVLVYDAMHREEEQLVSFEDAR
RSFERDYLVRVLKITGGNVTQAARLAKRNRTEFYKLLQRYQLDPSVFKQAQV

Specific function: Probable member of a two-component regulatory system yfhA/yfhK [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788905, Length=450, Percent_Identity=57.5555555555556, Blast_Score=503, Evalue=1e-144,
Organism=Escherichia coli, GI1790437, Length=435, Percent_Identity=44.8275862068966, Blast_Score=355, Evalue=2e-99,
Organism=Escherichia coli, GI1788550, Length=454, Percent_Identity=41.4096916299559, Blast_Score=345, Evalue=5e-96,
Organism=Escherichia coli, GI1790299, Length=476, Percent_Identity=38.655462184874, Blast_Score=309, Evalue=2e-85,
Organism=Escherichia coli, GI87082117, Length=315, Percent_Identity=46.6666666666667, Blast_Score=268, Evalue=8e-73,
Organism=Escherichia coli, GI1789233, Length=235, Percent_Identity=51.9148936170213, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1789087, Length=315, Percent_Identity=44.4444444444444, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI87082152, Length=323, Percent_Identity=38.6996904024768, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI1786524, Length=328, Percent_Identity=40.8536585365854, Blast_Score=211, Evalue=6e-56,
Organism=Escherichia coli, GI87081872, Length=236, Percent_Identity=43.6440677966102, Blast_Score=197, Evalue=8e-52,
Organism=Escherichia coli, GI1787583, Length=295, Percent_Identity=37.2881355932203, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1789828, Length=235, Percent_Identity=37.0212765957447, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI87081858, Length=294, Percent_Identity=32.312925170068, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1789809, Length=188, Percent_Identity=29.7872340425532, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1790552, Length=116, Percent_Identity=37.0689655172414, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1786599, Length=127, Percent_Identity=29.9212598425197, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1788394, Length=135, Percent_Identity=28.8888888888889, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI87082012, Length=116, Percent_Identity=33.6206896551724, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787375, Length=124, Percent_Identity=29.0322580645161, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50160; Mature: 50028

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDAKPRILIVDDDTDLLELLTMRLTAAGYKVDAVPSAEAALNYLDVSRPQLVISDMQMS
CCCCCCEEEEEECCHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHCCCCCCEEEECHHHC
GMDGMALFEQIHRSLPTLPVIILTAHGTIPDAVTAVQRGVFGYLAKPFDSKTLLANISQA
CCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LKFVPGAVSQKELSQAPWRKNIITQSAVMEDLLTRAGLVAEGDASVLIYGESGVGKELFA
HHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCHHHHH
HAIHDASKRSDHPFVAINCAAIPEQLLESELFGHVKGAFTGAVRDHKGLFQLAEGGTLFL
HHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEE
DEIGDMPVLLQVKLLRVLQERQVRPVGSAQSIPVDVRIISATHRDLKTEIAAGTFREDLY
HHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHH
YRLDVVSLTIPVLAQRREDIPLLVNYFLSTFSEKYQKNINGFSPEAMETLVAASWPGNVR
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHH
QLMNVVERCVALSTAPLISPVLVYDAMHREEEQLVSFEDARRSFERDYLVRVLKITGGNV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCH
TQAARLAKRNRTEFYKLLQRYQLDPSVFKQAQV
HHHHHHHHHCHHHHHHHHHHHCCCHHHHHHCCC
>Mature Secondary Structure 
SDAKPRILIVDDDTDLLELLTMRLTAAGYKVDAVPSAEAALNYLDVSRPQLVISDMQMS
CCCCCEEEEEECCHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHCCCCCCEEEECHHHC
GMDGMALFEQIHRSLPTLPVIILTAHGTIPDAVTAVQRGVFGYLAKPFDSKTLLANISQA
CCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LKFVPGAVSQKELSQAPWRKNIITQSAVMEDLLTRAGLVAEGDASVLIYGESGVGKELFA
HHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCHHHHH
HAIHDASKRSDHPFVAINCAAIPEQLLESELFGHVKGAFTGAVRDHKGLFQLAEGGTLFL
HHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEE
DEIGDMPVLLQVKLLRVLQERQVRPVGSAQSIPVDVRIISATHRDLKTEIAAGTFREDLY
HHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHH
YRLDVVSLTIPVLAQRREDIPLLVNYFLSTFSEKYQKNINGFSPEAMETLVAASWPGNVR
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHH
QLMNVVERCVALSTAPLISPVLVYDAMHREEEQLVSFEDARRSFERDYLVRVLKITGGNV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCH
TQAARLAKRNRTEFYKLLQRYQLDPSVFKQAQV
HHHHHHHHHCHHHHHHHHHHHCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]