Click here to switch to the map view.

The map label for this gene is cysB [H]

Identifier: 82702274

GI number: 82702274

Start: 1317373

End: 1318299

Strand: Reverse

Name: cysB [H]

Synonym: Nmul_A1145

Alternate gene names: 82702274

Gene position: 1318299-1317373 (Counterclockwise)

Preceding gene: 82702303

Following gene: 82702273

Centisome position: 41.4

GC content: 53.83

Gene sequence:

>927_bases
ATGAAATTGCAGCAATTACGTTATCTATGCGAAGTCGCCAACCAGGGCTTGAACTTATCCAAGGCGGCGGAAATTCTCCA
TACCTCCCAGCCCGGCATCAGCAAGCAGATCCGCCTGCTGGAAAACGAATTGGGGGTGGATATTTTTGTACGGAACGGCA
AGCGGGTGGTCGAAACCACGCCGCCCGGCCGGGCGATTCTTGAAATTGCCGAAAGAATGTTAAGAGACGCAAAAAACCTG
AAGCAGGTGGGGCAGGAATTTGCCAATGAAGCCAGCGGCTCTCTGACGATCGCGACGACCCATACACAAGCGCGGTATGC
ACTGCCTTCCGCAATTCAGCATTTTACCGCACGCTACCCCAAGGTGCGACTGGTTTTACGCCAGGGCAATCCGACACAGA
TCGCTGAACTGGTCACCTCCGGAGTGGCCGATATAGCGATTGCCACGGAAGCAATCGAACTGTTCAGCGAACTCGTCATG
CTGCCCTGCTACCAGTGGAACCGCTGCGTAATTGTTCCGCCTAGGCATCCGCTGCTCAAACTCAAGGGATTGACACTGGA
AGCAATCGCCGAGCATCCGATCATCACTTATGATTTTGCATTCACCGGCCGCTCGAAGATCAATCAGGCATTCGATGCAA
AAGGCCTGGTCCCCAATGTCGTGCTCACGGCGATTGATGCGGATGTCATTAAAACCTATGTGGAACTGGGACTGGGAATC
GGGATACTGGCGCAGATGGCGTTTGAACCTTCGCGCGACAGGCATTTGCGAGCCATTGACGCCAGCCATCTGTTCGAACC
CAGCACGACACGCATTGGTATCAGCCGCAACAGTTATCTGCGCAAATACGTATATGATTTCATCGAAATGTTTGCCCCCC
ATCTTGACCGGGCAACCGTTGCGGCGGCCATGACCGAACGTCCATAA

Upstream 100 bases:

>100_bases
GCATGATGCATCGAAAACGCGATAATAGCAATGAACATATATATCGTGAAAGAATATTATGTTAGATTAACATAACCAAA
CATTATTTAGATCACGACTC

Downstream 100 bases:

>100_bases
ACGGCAAATGGGCATAGCGGGTATAAGGTACATACGGGTACATAAGGGCACATAGGGTAGCGAACGGAAACAGCGTTTCA
CCCGTCCCCGTTTTACTGTT

Product: transcriptional regulator CysB-like protein

Products: NA

Alternate protein names: Cys regulon transcriptional activator [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MKLQQLRYLCEVANQGLNLSKAAEILHTSQPGISKQIRLLENELGVDIFVRNGKRVVETTPPGRAILEIAERMLRDAKNL
KQVGQEFANEASGSLTIATTHTQARYALPSAIQHFTARYPKVRLVLRQGNPTQIAELVTSGVADIAIATEAIELFSELVM
LPCYQWNRCVIVPPRHPLLKLKGLTLEAIAEHPIITYDFAFTGRSKINQAFDAKGLVPNVVLTAIDADVIKTYVELGLGI
GILAQMAFEPSRDRHLRAIDASHLFEPSTTRIGISRNSYLRKYVYDFIEMFAPHLDRATVAAAMTERP

Sequences:

>Translated_308_residues
MKLQQLRYLCEVANQGLNLSKAAEILHTSQPGISKQIRLLENELGVDIFVRNGKRVVETTPPGRAILEIAERMLRDAKNL
KQVGQEFANEASGSLTIATTHTQARYALPSAIQHFTARYPKVRLVLRQGNPTQIAELVTSGVADIAIATEAIELFSELVM
LPCYQWNRCVIVPPRHPLLKLKGLTLEAIAEHPIITYDFAFTGRSKINQAFDAKGLVPNVVLTAIDADVIKTYVELGLGI
GILAQMAFEPSRDRHLRAIDASHLFEPSTTRIGISRNSYLRKYVYDFIEMFAPHLDRATVAAAMTERP
>Mature_308_residues
MKLQQLRYLCEVANQGLNLSKAAEILHTSQPGISKQIRLLENELGVDIFVRNGKRVVETTPPGRAILEIAERMLRDAKNL
KQVGQEFANEASGSLTIATTHTQARYALPSAIQHFTARYPKVRLVLRQGNPTQIAELVTSGVADIAIATEAIELFSELVM
LPCYQWNRCVIVPPRHPLLKLKGLTLEAIAEHPIITYDFAFTGRSKINQAFDAKGLVPNVVLTAIDADVIKTYVELGLGI
GILAQMAFEPSRDRHLRAIDASHLFEPSTTRIGISRNSYLRKYVYDFIEMFAPHLDRATVAAAMTERP

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=307, Percent_Identity=55.3745928338762, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1788296, Length=290, Percent_Identity=48.2758620689655, Blast_Score=284, Evalue=5e-78,
Organism=Escherichia coli, GI157672245, Length=236, Percent_Identity=30.5084745762712, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1788887, Length=245, Percent_Identity=31.4285714285714, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790399, Length=270, Percent_Identity=28.8888888888889, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI2367136, Length=242, Percent_Identity=25.2066115702479, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788481, Length=249, Percent_Identity=26.9076305220884, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789204, Length=241, Percent_Identity=25.7261410788382, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790208, Length=261, Percent_Identity=26.0536398467433, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1788748, Length=257, Percent_Identity=25.6809338521401, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787879, Length=257, Percent_Identity=27.6264591439689, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1787601, Length=149, Percent_Identity=26.8456375838926, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1787806, Length=173, Percent_Identity=25.4335260115607, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34269; Mature: 34269

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQQLRYLCEVANQGLNLSKAAEILHTSQPGISKQIRLLENELGVDIFVRNGKRVVETT
CCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEEECCCEEEEEC
PPGRAILEIAERMLRDAKNLKQVGQEFANEASGSLTIATTHTQARYALPSAIQHFTARYP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHCCC
KVRLVLRQGNPTQIAELVTSGVADIAIATEAIELFSELVMLPCYQWNRCVIVPPRHPLLK
EEEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEE
LKGLTLEAIAEHPIITYDFAFTGRSKINQAFDAKGLVPNVVLTAIDADVIKTYVELGLGI
ECCCCHHHHHCCCEEEEEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCH
GILAQMAFEPSRDRHLRAIDASHLFEPSTTRIGISRNSYLRKYVYDFIEMFAPHLDRATV
HHHHHHHCCCCCCCCEEEECHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCHHHH
AAAMTERP
HHHHCCCC
>Mature Secondary Structure
MKLQQLRYLCEVANQGLNLSKAAEILHTSQPGISKQIRLLENELGVDIFVRNGKRVVETT
CCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEEECCCEEEEEC
PPGRAILEIAERMLRDAKNLKQVGQEFANEASGSLTIATTHTQARYALPSAIQHFTARYP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHCCC
KVRLVLRQGNPTQIAELVTSGVADIAIATEAIELFSELVMLPCYQWNRCVIVPPRHPLLK
EEEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEE
LKGLTLEAIAEHPIITYDFAFTGRSKINQAFDAKGLVPNVVLTAIDADVIKTYVELGLGI
ECCCCHHHHHCCCEEEEEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCH
GILAQMAFEPSRDRHLRAIDASHLFEPSTTRIGISRNSYLRKYVYDFIEMFAPHLDRATV
HHHHHHHCCCCCCCCEEEECHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCHHHH
AAAMTERP
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]