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The map label for this gene is nuoB [H]

Identifier: 82702221

GI number: 82702221

Start: 1258283

End: 1258759

Strand: Direct

Name: nuoB [H]

Synonym: Nmul_A1092

Alternate gene names: 82702221

Gene position: 1258283-1258759 (Clockwise)

Preceding gene: 82702220

Following gene: 82702222

Centisome position: 39.52

GC content: 55.77

Gene sequence:

>477_bases
ATGAGTACGAACGGAGTAATGGAAAAAGGCTTTGTCACTACCAGCCTTGACTCGGTCATCAACTGGGGGCGTACCGGCTC
GATGTGGCCGATGACTTTTGGCCTGGCATGCTGCGCCGTCGAAATGATGCAGGCAGGCGCATCCCGTTACGATCTCGACC
GCTTTGGAATTGTGTTTCGTCCCAGCCCGCGTCAGTCGGACGTCATGATTGTTGCCGGAACCCTTTGTAATAAAATGGCG
CCTGCCCTCCGCAAGGTGTATGACCAGATGGCGGAGCCGCGCTGGGTGATATCCATGGGCTCCTGCGCCAATGGCGGCGG
TTATTATCATTACTCCTACTCGGTGGTGCGTGGATGTGATCGTATCGTCCCGGTCGATATTTATGTTCCCGGTTGCCCGC
CTACCGCAGAAGCCCTGCTGTACGGCATTATCCAGCTCCAGAACAAGATCCATCGCACCAATACCATCGCTCGTTAG

Upstream 100 bases:

>100_bases
GGTCTGTTCGGTTTTATTGCAATGATGGTTTTTCTCGGCATCCTTGTCGTCGGCTTCATCTACGAGTGGACGAAGGGCGC
TCTGGAGTGGGATTGAGGCC

Downstream 100 bases:

>100_bases
GCTCATCTATGTCGTCCACTCGTCTGGAAACGCTCGCCCTTTGCCTTCAGAATGTGTTGGCGGATAAACTTGCCAACGTC
GATCAGCAACTGGACCAGCT

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MSTNGVMEKGFVTTSLDSVINWGRTGSMWPMTFGLACCAVEMMQAGASRYDLDRFGIVFRPSPRQSDVMIVAGTLCNKMA
PALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGIIQLQNKIHRTNTIAR

Sequences:

>Translated_158_residues
MSTNGVMEKGFVTTSLDSVINWGRTGSMWPMTFGLACCAVEMMQAGASRYDLDRFGIVFRPSPRQSDVMIVAGTLCNKMA
PALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGIIQLQNKIHRTNTIAR
>Mature_157_residues
STNGVMEKGFVTTSLDSVINWGRTGSMWPMTFGLACCAVEMMQAGASRYDLDRFGIVFRPSPRQSDVMIVAGTLCNKMAP
ALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGIIQLQNKIHRTNTIAR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=146, Percent_Identity=79.4520547945205, Blast_Score=257, Evalue=3e-69,
Organism=Escherichia coli, GI1788624, Length=145, Percent_Identity=51.7241379310345, Blast_Score=166, Evalue=6e-43,
Organism=Escherichia coli, GI1789074, Length=119, Percent_Identity=42.8571428571429, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788834, Length=126, Percent_Identity=38.8888888888889, Blast_Score=93, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17509685, Length=149, Percent_Identity=69.1275167785235, Blast_Score=238, Evalue=1e-63,
Organism=Drosophila melanogaster, GI18859983, Length=141, Percent_Identity=80.1418439716312, Blast_Score=258, Evalue=8e-70,
Organism=Drosophila melanogaster, GI24642371, Length=141, Percent_Identity=80.1418439716312, Blast_Score=258, Evalue=8e-70,
Organism=Drosophila melanogaster, GI24651058, Length=146, Percent_Identity=77.3972602739726, Blast_Score=253, Evalue=4e-68,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 17429; Mature: 17298

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
6.3 %Met     (Translated Protein)
10.1 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
9.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTNGVMEKGFVTTSLDSVINWGRTGSMWPMTFGLACCAVEMMQAGASRYDLDRFGIVFR
CCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEC
PSPRQSDVMIVAGTLCNKMAPALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCD
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEHHHHHCCC
RIVPVDIYVPGCPPTAEALLYGIIQLQNKIHRTNTIAR
CEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
STNGVMEKGFVTTSLDSVINWGRTGSMWPMTFGLACCAVEMMQAGASRYDLDRFGIVFR
CCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEC
PSPRQSDVMIVAGTLCNKMAPALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCD
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEHHHHHCCC
RIVPVDIYVPGCPPTAEALLYGIIQLQNKIHRTNTIAR
CEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA