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The map label for this gene is ilvE [H]

Identifier: 82702189

GI number: 82702189

Start: 1225735

End: 1226652

Strand: Direct

Name: ilvE [H]

Synonym: Nmul_A1060

Alternate gene names: 82702189

Gene position: 1225735-1226652 (Clockwise)

Preceding gene: 82702188

Following gene: 82702190

Centisome position: 38.49

GC content: 52.61

Gene sequence:

>918_bases
ATGTCAATGGCTGACCGCGACGGTGTGATCTGGAGTGATGGAAAGATGATTCCGTGGCGTGAGGCTACCACACATGTACT
CACCCACACCCTGCATTATGGGATGGGGGTATTCGAAGGATTGCGTGCCTACGAAACCCCCCGCGGATCTGCAATTTTCC
GTCTGAAAGAACACACCGATCGTTTGTTCAATTCCGCGCACATCTTCATGATGAAGATGCCCTATGACAAGGCGACGCTG
ATACAGGCGCAGTGCGATGTCGTAAGGCAGAACGATCTGAAGTCGTGTTATATCCGTCCCATCGTGTTTTATGGTTCCGA
AGCCATGGGCATTTCAGCTAAAACGCTTTCGGTGCACGTGGCTATTGCAGCTTGGGCGTGGGGCACGTATCTTGGTCCTG
ATGGCCTCGAAAAAGGCATCCGTGTCAAGACTTCGTCATTTACGCGGCATCATGTGAATATCAATATGTGCCGTGCCAAG
TCGGTCACGACCTATGCAAATTCCATCCTCGCGCATCAGGAGGTAGCGCATGATGGCTATGATGAGGCGCTGCTTCTCGA
TGTGGACGGCTATGTTGCTGAAGGGGCTGGTGAAAACATATTCATCGTGAAGCAGGGCAAATTGTATACGCCTGACTTGA
CTTCCTGTCTGGAAGGCATTACGCGCGCATCTCTCATAGAGCTTGCGGAAGAAATCGGAATCCCGGTTATCGAGAAGCGC
ATCACCCGCGATGAAGTCTATTGCGCGGATGAAGCCTTTTTCACCGGCACCGCAGCCGAGGTAACACCAATCAGGGAACT
GGATAACCGCACGATCGGCAGCGGCAGGCGTGGTCCTATTACAGAAAAGCTCCAGGCCCTCTTTTTTGAATGTGCCAGAG
GCAACGGCAAACATGCCGAGTGGCTCACCCATGTCTGA

Upstream 100 bases:

>100_bases
AAACAGTCTGCTTTCCTGTACCCCTTCTCTCGAAAGGGAATCCCGAGGATCAGGAAATGCGTTAGAATATACGAACTTAA
ATTCTCTCCGGAGTTTGTGA

Downstream 100 bases:

>100_bases
TAAAACGTTATATCCTCACCGATACATTTAAGCGATACGTCCAGGAAAATCTGATGCAGAAGCTTGCCTCCAATACCCAG
CGCGAGATCGAGGTGACACC

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MSMADRDGVIWSDGKMIPWREATTHVLTHTLHYGMGVFEGLRAYETPRGSAIFRLKEHTDRLFNSAHIFMMKMPYDKATL
IQAQCDVVRQNDLKSCYIRPIVFYGSEAMGISAKTLSVHVAIAAWAWGTYLGPDGLEKGIRVKTSSFTRHHVNINMCRAK
SVTTYANSILAHQEVAHDGYDEALLLDVDGYVAEGAGENIFIVKQGKLYTPDLTSCLEGITRASLIELAEEIGIPVIEKR
ITRDEVYCADEAFFTGTAAEVTPIRELDNRTIGSGRRGPITEKLQALFFECARGNGKHAEWLTHV

Sequences:

>Translated_305_residues
MSMADRDGVIWSDGKMIPWREATTHVLTHTLHYGMGVFEGLRAYETPRGSAIFRLKEHTDRLFNSAHIFMMKMPYDKATL
IQAQCDVVRQNDLKSCYIRPIVFYGSEAMGISAKTLSVHVAIAAWAWGTYLGPDGLEKGIRVKTSSFTRHHVNINMCRAK
SVTTYANSILAHQEVAHDGYDEALLLDVDGYVAEGAGENIFIVKQGKLYTPDLTSCLEGITRASLIELAEEIGIPVIEKR
ITRDEVYCADEAFFTGTAAEVTPIRELDNRTIGSGRRGPITEKLQALFFECARGNGKHAEWLTHV
>Mature_304_residues
SMADRDGVIWSDGKMIPWREATTHVLTHTLHYGMGVFEGLRAYETPRGSAIFRLKEHTDRLFNSAHIFMMKMPYDKATLI
QAQCDVVRQNDLKSCYIRPIVFYGSEAMGISAKTLSVHVAIAAWAWGTYLGPDGLEKGIRVKTSSFTRHHVNINMCRAKS
VTTYANSILAHQEVAHDGYDEALLLDVDGYVAEGAGENIFIVKQGKLYTPDLTSCLEGITRASLIELAEEIGIPVIEKRI
TRDEVYCADEAFFTGTAAEVTPIRELDNRTIGSGRRGPITEKLQALFFECARGNGKHAEWLTHV

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=299, Percent_Identity=44.4816053511706, Blast_Score=265, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI17565728, Length=285, Percent_Identity=29.4736842105263, Blast_Score=87, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6322002, Length=285, Percent_Identity=26.3157894736842, Blast_Score=72, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6322608, Length=327, Percent_Identity=25.6880733944954, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 34016; Mature: 33885

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMADRDGVIWSDGKMIPWREATTHVLTHTLHYGMGVFEGLRAYETPRGSAIFRLKEHTD
CCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHH
RLFNSAHIFMMKMPYDKATLIQAQCDVVRQNDLKSCYIRPIVFYGSEAMGISAKTLSVHV
HHHCCEEEEEEECCCCCCEEEHHHHHHHHHCCCCCEEEEEEEEECCCCCCCEEEEEEEEE
AIAAWAWGTYLGPDGLEKGIRVKTSSFTRHHVNINMCRAKSVTTYANSILAHQEVAHDGY
EEEEECCCCCCCCHHHCCCCEEEECCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHCCCC
DEALLLDVDGYVAEGAGENIFIVKQGKLYTPDLTSCLEGITRASLIELAEEIGIPVIEKR
CCEEEEEECCEEECCCCCEEEEEECCCEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ITRDEVYCADEAFFTGTAAEVTPIRELDNRTIGSGRRGPITEKLQALFFECARGNGKHAE
CCCCCEEECCCCEECCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCH
WLTHV
HHCCC
>Mature Secondary Structure 
SMADRDGVIWSDGKMIPWREATTHVLTHTLHYGMGVFEGLRAYETPRGSAIFRLKEHTD
CCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHH
RLFNSAHIFMMKMPYDKATLIQAQCDVVRQNDLKSCYIRPIVFYGSEAMGISAKTLSVHV
HHHCCEEEEEEECCCCCCEEEHHHHHHHHHCCCCCEEEEEEEEECCCCCCCEEEEEEEEE
AIAAWAWGTYLGPDGLEKGIRVKTSSFTRHHVNINMCRAKSVTTYANSILAHQEVAHDGY
EEEEECCCCCCCCHHHCCCCEEEECCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHCCCC
DEALLLDVDGYVAEGAGENIFIVKQGKLYTPDLTSCLEGITRASLIELAEEIGIPVIEKR
CCEEEEEECCEEECCCCCEEEEEECCCEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ITRDEVYCADEAFFTGTAAEVTPIRELDNRTIGSGRRGPITEKLQALFFECARGNGKHAE
CCCCCEEECCCCEECCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCH
WLTHV
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]