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The map label for this gene is yafV [C]

Identifier: 82702185

GI number: 82702185

Start: 1216157

End: 1217017

Strand: Reverse

Name: yafV [C]

Synonym: Nmul_A1056

Alternate gene names: 82702185

Gene position: 1217017-1216157 (Counterclockwise)

Preceding gene: 82702186

Following gene: 82702184

Centisome position: 38.22

GC content: 58.19

Gene sequence:

>861_bases
ATGCAAGACTCCCTAGCCTGTAACTCACTGCCTCCTGAAACAGTTCCCACGGGCCCGGTTCGTGTTGCGGCCATTCAGAT
GGCGGCAGGCCCGAATATCTACGCCAATCTGGAAGAAGCCGGACGGCTTCTGGATATTGCTGCATCACGCGGCGCGAAGC
TGGCGGCGCTCCCCGAGTATTTCTGCCTGATGGGAATGGAGGATGCCGATAGAGTAGCGGCCCGTGAGCAGGATAATCAG
GGGCCGATACAGGAATTCCTGGGCAATACTGCCAAGCGTCTCGGCATATGGCTGGTAGGCGGTTCAGTACCCCTGGTTTC
TTCCCGGCCTGACAAGGTGCGAAACAGTTGCCTGGTATACAACGACAACGGTGAGCAGGTCGCACGGTACGACAAAATAC
ATTTGTTCGGCCTGGAGCTGGGAACTGAACGCTATGCCGAAGAAGAAACCATCGAGGCAGGCCAGGGTGTCGTTGCCCTG
GAATCCCCCTTTGGCCGCATCGGACTTTCCATCTGCTACGATATCCGCTTTCCCGAGCTTTACCGTTCCATGGGGCAGGT
AGACATTATCTTCGCCCCGGCGGCTTTCACCGCCACGACGGGCAAGGCGCATTGGGAAACCTTGATTCGTGCCCGGGCCA
TCGAAAATCTGGCATACGTCGTTGCGCCTGCTCAGGGTGGATACCATATCAATGGGCGGGAAACCCATGGGGACAGCATG
ATAGTCGATCCCTGGGGCGTGGTGCTGGACCGCCTCCCGCGCGGACCCGGAGTTGTCATCGCGGATATTGATCCTGAATA
TCAGGCGAGCTTGCGCAGCAGCCTGCCCGCCTTGAATCACCGCACGCTGCGTTACTTTTGA

Upstream 100 bases:

>100_bases
CTGTGGCCAATGTCGGATTCGGTATCAACCACAATACTTTATAAAGACAATCTTTTAAGTTACCCTGTATTCTCCGCTCC
CCGAAAAATACACCTCAAAT

Downstream 100 bases:

>100_bases
ATTCCGTTGAACCTTGTTCTCCTTCTCCCCCACTAACTACCATGATTACAGAACAGACTATCCAGGTTAACGATCTTTTC
GCGACGGCCAACAATTGTCT

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: carboxylate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MQDSLACNSLPPETVPTGPVRVAAIQMAAGPNIYANLEEAGRLLDIAASRGAKLAALPEYFCLMGMEDADRVAAREQDNQ
GPIQEFLGNTAKRLGIWLVGGSVPLVSSRPDKVRNSCLVYNDNGEQVARYDKIHLFGLELGTERYAEEETIEAGQGVVAL
ESPFGRIGLSICYDIRFPELYRSMGQVDIIFAPAAFTATTGKAHWETLIRARAIENLAYVVAPAQGGYHINGRETHGDSM
IVDPWGVVLDRLPRGPGVVIADIDPEYQASLRSSLPALNHRTLRYF

Sequences:

>Translated_286_residues
MQDSLACNSLPPETVPTGPVRVAAIQMAAGPNIYANLEEAGRLLDIAASRGAKLAALPEYFCLMGMEDADRVAAREQDNQ
GPIQEFLGNTAKRLGIWLVGGSVPLVSSRPDKVRNSCLVYNDNGEQVARYDKIHLFGLELGTERYAEEETIEAGQGVVAL
ESPFGRIGLSICYDIRFPELYRSMGQVDIIFAPAAFTATTGKAHWETLIRARAIENLAYVVAPAQGGYHINGRETHGDSM
IVDPWGVVLDRLPRGPGVVIADIDPEYQASLRSSLPALNHRTLRYF
>Mature_286_residues
MQDSLACNSLPPETVPTGPVRVAAIQMAAGPNIYANLEEAGRLLDIAASRGAKLAALPEYFCLMGMEDADRVAAREQDNQ
GPIQEFLGNTAKRLGIWLVGGSVPLVSSRPDKVRNSCLVYNDNGEQVARYDKIHLFGLELGTERYAEEETIEAGQGVVAL
ESPFGRIGLSICYDIRFPELYRSMGQVDIIFAPAAFTATTGKAHWETLIRARAIENLAYVVAPAQGGYHINGRETHGDSM
IVDPWGVVLDRLPRGPGVVIADIDPEYQASLRSSLPALNHRTLRYF

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI5031947, Length=267, Percent_Identity=37.8277153558052, Blast_Score=170, Evalue=1e-42,
Organism=Homo sapiens, GI297632348, Length=267, Percent_Identity=37.8277153558052, Blast_Score=170, Evalue=1e-42,
Organism=Homo sapiens, GI297632350, Length=267, Percent_Identity=37.8277153558052, Blast_Score=170, Evalue=1e-42,
Organism=Homo sapiens, GI9910460, Length=264, Percent_Identity=32.9545454545455, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI297632346, Length=192, Percent_Identity=32.2916666666667, Blast_Score=93, Evalue=2e-19,
Organism=Escherichia coli, GI1786412, Length=217, Percent_Identity=32.7188940092166, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17556280, Length=280, Percent_Identity=37.8571428571429, Blast_Score=172, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17533173, Length=276, Percent_Identity=26.4492753623188, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323383, Length=276, Percent_Identity=31.8840579710145, Blast_Score=153, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6322335, Length=302, Percent_Identity=31.1258278145695, Blast_Score=137, Evalue=2e-33,
Organism=Drosophila melanogaster, GI17933642, Length=266, Percent_Identity=36.8421052631579, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI21355835, Length=272, Percent_Identity=32.7205882352941, Blast_Score=139, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.5.1

Molecular weight: Translated: 31184; Mature: 31184

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDSLACNSLPPETVPTGPVRVAAIQMAAGPNIYANLEEAGRLLDIAASRGAKLAALPEY
CCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEEEHHHHCCEEEEHHCCCCEEEECHHH
FCLMGMEDADRVAAREQDNQGPIQEFLGNTAKRLGIWLVGGSVPLVSSRPDKVRNSCLVY
HHHCCCCCHHHHHHHCCCCCCHHHHHHCCHHHHEEEEEECCCCCCCCCCCHHHHCCEEEE
NDNGEQVARYDKIHLFGLELGTERYAEEETIEAGQGVVALESPFGRIGLSICYDIRFPEL
CCCCCEEHEECEEEEEEEECCCHHHCCHHHHHCCCCEEEECCCCCCCCEEEEEECCCHHH
YRSMGQVDIIFAPAAFTATTGKAHWETLIRARAIENLAYVVAPAQGGYHINGRETHGDSM
HHHCCCEEEEEECCEEEECCCHHHHHHHHHHHHHHCEEEEEEECCCCEEECCCCCCCCCE
IVDPWGVVLDRLPRGPGVVIADIDPEYQASLRSSLPALNHRTLRYF
EECHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCCCCEEECC
>Mature Secondary Structure
MQDSLACNSLPPETVPTGPVRVAAIQMAAGPNIYANLEEAGRLLDIAASRGAKLAALPEY
CCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEEEHHHHCCEEEEHHCCCCEEEECHHH
FCLMGMEDADRVAAREQDNQGPIQEFLGNTAKRLGIWLVGGSVPLVSSRPDKVRNSCLVY
HHHCCCCCHHHHHHHCCCCCCHHHHHHCCHHHHEEEEEECCCCCCCCCCCHHHHCCEEEE
NDNGEQVARYDKIHLFGLELGTERYAEEETIEAGQGVVALESPFGRIGLSICYDIRFPEL
CCCCCEEHEECEEEEEEEECCCHHHCCHHHHHCCCCEEEECCCCCCCCEEEEEECCCHHH
YRSMGQVDIIFAPAAFTATTGKAHWETLIRARAIENLAYVVAPAQGGYHINGRETHGDSM
HHHCCCEEEEEECCEEEECCCHHHHHHHHHHHHHHCEEEEEEECCCCEEECCCCCCCCCE
IVDPWGVVLDRLPRGPGVVIADIDPEYQASLRSSLPALNHRTLRYF
EECHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nitrile; H2O

Specific reaction: A nitrile + H2O = a carboxylate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]