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The map label for this gene is gatA [H]

Identifier: 82702073

GI number: 82702073

Start: 1094498

End: 1096384

Strand: Direct

Name: gatA [H]

Synonym: Nmul_A0944

Alternate gene names: 82702073

Gene position: 1094498-1096384 (Clockwise)

Preceding gene: 82702072

Following gene: 82702076

Centisome position: 34.37

GC content: 57.23

Gene sequence:

>1887_bases
ATGAACAAGCTCGATCTCCTGGGCGATATCCCGTCCCTGCTGCAACGTTATGTCACTGGGGAACTGACTCCTACCGGGAT
GGTAGAGGCGGTGCATGCGCTGATCCATGACGATCCCGACAACGTGTGGATACACAAGCTGCCGCTGGAATCTCTGCGGC
AATATGCCCGGAAAGTGGAAGAAAGAGGCCTGGCTGCACTGCCTTTATATGGTGTTCCCTTCGCTATCAAGGATAATATC
GATCTCGCGGGCGTTCCAACTACTGCGGCTTGCCCGGCGTTTGCCTACACACCGCCACGCAGCGCAACGGTGGTGCAGAG
GCTGATCGACGCAGGCGCGATTCCCATCGGCAAGACCAATCTCGATCAGTTTGCCACCGGGTTGAATGGTACCCGCTCTC
CCTATGGCGTCTGCCGCAACGCCTTCAATCCCGAATACATCTCCGGCGGATCAAGCTCGGGTTCAGCAGTGGCTGTAGCG
AAAGGCTGGGCATGTTTCAGCCTGGGAACCGATACCGCAGGCTCCGGCAGAGTACCCGCTGCCTTCAATAACCTTATCGG
GCATAAACCCACCCGGGGTTGGCTTTCCACGCGCGGTGTCGTCCCCGCCTGCCGCTCGCTGGATTGCGTGTCCATCTTCG
CCTTTACTGCTGCTGATGCGGAGCGAGTCCTCGAAGCAGCCGCAAGCTATGATGAGGAAGACATCTATTCCCGCCAGAGA
GGGAGCAGCGATGATCCTGCCTTGCAAAACCTTAGACAGCGTTTCCGTTCTGGCATACCGCCCCGGCAGCAATTGCAATT
CTTTGGCAACTTGGAGAGTGCCAGGTTATTCGAGGAAGCAGTAACGCAACTGCAAGCATTGGGCGGTGAGGTCATCGAGA
TCGATTTTTCGCCCTTTCTGGAAGCAGCGCACCTGCTTTATGGGGGGCCATGGGTAGCCGAGCGTTATGCCGCCATTCAG
CAATTTTTTGATCTGCATAGCGATGAGGTAATTTCGCCGGTACAAGAGATCATTGCGGGCGCACACCGATTCTCGGCTGC
AGATGCCTACAACGGCTTGTATCGCTTGCGTACGCTGAAACGTCAGGCTGACCGTATCTGGTCCGAAGTGGATTGCCTGT
TGACGCCTACCGCTGGGACCATTTACCGCATAGAGGAAATGCTGGCAGACCCGATTCGGCTGAACACGAATCTGGGTTAC
TACACCAATTACATAAATCTGCTCGACTATTCAGCAGTGGCAGTGCCCGCCGGATTTCAGAAGGATGGATTGCCGTTTGG
CGTAACCCTGGTGGCTCCAGCTCATCAGGACGGCTCGCTGTTGCATCTTGCCGCACGTCTGCAACAGGTGTACGCGCTAC
CGCTGGGGGCTACCGATACTCCACTGGAAAAAGAAGTTTTGACAGAAAAGGGATCTTTTCAACGACCGCCACCGCCGGAG
CAACCAGCCCTCGTGCGTGTGGCGGTCTGCGGCGCGCATTTGTCCGGTCTGCCATTGAATCCTCAACTGACCAGCCGCAA
CGGACGCCTGGTAGCAAGTACCACAACCTCCCCCGACTACAAATTCTACGCGCTTCCCGGAATACCGCCGCTGCGTCCCG
GATTGGTGCGCGTGGATAAGAACGAGCGAGGAGCCGCGATTGAAGTCGAGGTGTGGGAACTGCCAGCAAGCGAATTCGGC
AGCTTTGTGGCGGGCATACCTGCTCCGCTGGGAATCGGCACCATTACGCTCGCGGGGGGCGAATCCGTGCAGGGTTTCTT
GTGCGAGGGCTACGCGGTAGGGGATGCAAGGGATATCAGTCAATTCGGAGGATGGCGCCGCTATATGCAATCAACGGCTG
ATCCCATTGCAAGCGAAGAGGTGGTGCGCGAGGTGGATCCGGGATAA

Upstream 100 bases:

>100_bases
TGGAGACACCGGTCAGCGGTAGGGTACGACAGCTATTCTGCAAGGAGGGGAGCCATATATCCGCCGGGCAGATGTTGCTT
ATCGTTCAGGAGGAATGAAT

Downstream 100 bases:

>100_bases
GGATGACGTATTCTTCCGCTCCCGGATGGGTTAGGTCCTATAGGTCCTCGCCTCGCCCATTCCTGCTCCGAAGCTGATGT
ACATCGGGAAGGAGCCATAA

Product: allophanate hydrolase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 628; Mature: 628

Protein sequence:

>628_residues
MNKLDLLGDIPSLLQRYVTGELTPTGMVEAVHALIHDDPDNVWIHKLPLESLRQYARKVEERGLAALPLYGVPFAIKDNI
DLAGVPTTAACPAFAYTPPRSATVVQRLIDAGAIPIGKTNLDQFATGLNGTRSPYGVCRNAFNPEYISGGSSSGSAVAVA
KGWACFSLGTDTAGSGRVPAAFNNLIGHKPTRGWLSTRGVVPACRSLDCVSIFAFTAADAERVLEAAASYDEEDIYSRQR
GSSDDPALQNLRQRFRSGIPPRQQLQFFGNLESARLFEEAVTQLQALGGEVIEIDFSPFLEAAHLLYGGPWVAERYAAIQ
QFFDLHSDEVISPVQEIIAGAHRFSAADAYNGLYRLRTLKRQADRIWSEVDCLLTPTAGTIYRIEEMLADPIRLNTNLGY
YTNYINLLDYSAVAVPAGFQKDGLPFGVTLVAPAHQDGSLLHLAARLQQVYALPLGATDTPLEKEVLTEKGSFQRPPPPE
QPALVRVAVCGAHLSGLPLNPQLTSRNGRLVASTTTSPDYKFYALPGIPPLRPGLVRVDKNERGAAIEVEVWELPASEFG
SFVAGIPAPLGIGTITLAGGESVQGFLCEGYAVGDARDISQFGGWRRYMQSTADPIASEEVVREVDPG

Sequences:

>Translated_628_residues
MNKLDLLGDIPSLLQRYVTGELTPTGMVEAVHALIHDDPDNVWIHKLPLESLRQYARKVEERGLAALPLYGVPFAIKDNI
DLAGVPTTAACPAFAYTPPRSATVVQRLIDAGAIPIGKTNLDQFATGLNGTRSPYGVCRNAFNPEYISGGSSSGSAVAVA
KGWACFSLGTDTAGSGRVPAAFNNLIGHKPTRGWLSTRGVVPACRSLDCVSIFAFTAADAERVLEAAASYDEEDIYSRQR
GSSDDPALQNLRQRFRSGIPPRQQLQFFGNLESARLFEEAVTQLQALGGEVIEIDFSPFLEAAHLLYGGPWVAERYAAIQ
QFFDLHSDEVISPVQEIIAGAHRFSAADAYNGLYRLRTLKRQADRIWSEVDCLLTPTAGTIYRIEEMLADPIRLNTNLGY
YTNYINLLDYSAVAVPAGFQKDGLPFGVTLVAPAHQDGSLLHLAARLQQVYALPLGATDTPLEKEVLTEKGSFQRPPPPE
QPALVRVAVCGAHLSGLPLNPQLTSRNGRLVASTTTSPDYKFYALPGIPPLRPGLVRVDKNERGAAIEVEVWELPASEFG
SFVAGIPAPLGIGTITLAGGESVQGFLCEGYAVGDARDISQFGGWRRYMQSTADPIASEEVVREVDPG
>Mature_628_residues
MNKLDLLGDIPSLLQRYVTGELTPTGMVEAVHALIHDDPDNVWIHKLPLESLRQYARKVEERGLAALPLYGVPFAIKDNI
DLAGVPTTAACPAFAYTPPRSATVVQRLIDAGAIPIGKTNLDQFATGLNGTRSPYGVCRNAFNPEYISGGSSSGSAVAVA
KGWACFSLGTDTAGSGRVPAAFNNLIGHKPTRGWLSTRGVVPACRSLDCVSIFAFTAADAERVLEAAASYDEEDIYSRQR
GSSDDPALQNLRQRFRSGIPPRQQLQFFGNLESARLFEEAVTQLQALGGEVIEIDFSPFLEAAHLLYGGPWVAERYAAIQ
QFFDLHSDEVISPVQEIIAGAHRFSAADAYNGLYRLRTLKRQADRIWSEVDCLLTPTAGTIYRIEEMLADPIRLNTNLGY
YTNYINLLDYSAVAVPAGFQKDGLPFGVTLVAPAHQDGSLLHLAARLQQVYALPLGATDTPLEKEVLTEKGSFQRPPPPE
QPALVRVAVCGAHLSGLPLNPQLTSRNGRLVASTTTSPDYKFYALPGIPPLRPGLVRVDKNERGAAIEVEVWELPASEFG
SFVAGIPAPLGIGTITLAGGESVQGFLCEGYAVGDARDISQFGGWRRYMQSTADPIASEEVVREVDPG

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=494, Percent_Identity=26.9230769230769, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI195972892, Length=419, Percent_Identity=24.3436754176611, Blast_Score=89, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17543272, Length=431, Percent_Identity=32.0185614849188, Blast_Score=155, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17537465, Length=484, Percent_Identity=24.7933884297521, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17556264, Length=242, Percent_Identity=28.9256198347107, Blast_Score=78, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17538252, Length=133, Percent_Identity=30.8270676691729, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71990152, Length=125, Percent_Identity=34.4, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=582, Percent_Identity=45.1890034364261, Blast_Score=443, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6323950, Length=170, Percent_Identity=41.1764705882353, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24648113, Length=497, Percent_Identity=28.5714285714286, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24652985, Length=423, Percent_Identity=26.9503546099291, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19922090, Length=423, Percent_Identity=26.9503546099291, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24652981, Length=423, Percent_Identity=26.9503546099291, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24652983, Length=423, Percent_Identity=26.9503546099291, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24644968, Length=413, Percent_Identity=25.181598062954, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI21356731, Length=448, Percent_Identity=24.7767857142857, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI161078093, Length=423, Percent_Identity=23.8770685579196, Blast_Score=67, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 67836; Mature: 67836

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLDLLGDIPSLLQRYVTGELTPTGMVEAVHALIHDDPDNVWIHKLPLESLRQYARKVE
CCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHH
ERGLAALPLYGVPFAIKDNIDLAGVPTTAACPAFAYTPPRSATVVQRLIDAGAIPIGKTN
HCCCEEECCCCCCEEEECCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCC
LDQFATGLNGTRSPYGVCRNAFNPEYISGGSSSGSAVAVAKGWACFSLGTDTAGSGRVPA
HHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCEEEEECCEEEEECCCCCCCCCCCCH
AFNNLIGHKPTRGWLSTRGVVPACRSLDCVSIFAFTAADAERVLEAAASYDEEDIYSRQR
HHHHHHCCCCCCCHHHCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHC
GSSDDPALQNLRQRFRSGIPPRQQLQFFGNLESARLFEEAVTQLQALGGEVIEIDFSPFL
CCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEECCHHHH
EAAHLLYGGPWVAERYAAIQQFFDLHSDEVISPVQEIIAGAHRFSAADAYNGLYRLRTLK
HHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQADRIWSEVDCLLTPTAGTIYRIEEMLADPIRLNTNLGYYTNYINLLDYSAVAVPAGFQ
HHHHHHHHHCCEEECCCCCHHHHHHHHHCCCEEECCCCCHHHHHHHHHHCCCEECCCCCC
KDGLPFGVTLVAPAHQDGSLLHLAARLQQVYALPLGATDTPLEKEVLTEKGSFQRPPPPE
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCC
QPALVRVAVCGAHLSGLPLNPQLTSRNGRLVASTTTSPDYKFYALPGIPPLRPGLVRVDK
CCCEEEEEEECCHHCCCCCCCCEECCCCEEEEEECCCCCEEEEECCCCCCCCCCEEEECC
NERGAAIEVEVWELPASEFGSFVAGIPAPLGIGTITLAGGESVQGFLCEGYAVGDARDIS
CCCCCEEEEEEEECCHHHHHHHHHCCCCCCCCCEEEECCCCCCCCEEECCEECCCHHHHH
QFGGWRRYMQSTADPIASEEVVREVDPG
HHHHHHHHHHHHCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MNKLDLLGDIPSLLQRYVTGELTPTGMVEAVHALIHDDPDNVWIHKLPLESLRQYARKVE
CCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHH
ERGLAALPLYGVPFAIKDNIDLAGVPTTAACPAFAYTPPRSATVVQRLIDAGAIPIGKTN
HCCCEEECCCCCCEEEECCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCC
LDQFATGLNGTRSPYGVCRNAFNPEYISGGSSSGSAVAVAKGWACFSLGTDTAGSGRVPA
HHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCEEEEECCEEEEECCCCCCCCCCCCH
AFNNLIGHKPTRGWLSTRGVVPACRSLDCVSIFAFTAADAERVLEAAASYDEEDIYSRQR
HHHHHHCCCCCCCHHHCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHC
GSSDDPALQNLRQRFRSGIPPRQQLQFFGNLESARLFEEAVTQLQALGGEVIEIDFSPFL
CCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEECCHHHH
EAAHLLYGGPWVAERYAAIQQFFDLHSDEVISPVQEIIAGAHRFSAADAYNGLYRLRTLK
HHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQADRIWSEVDCLLTPTAGTIYRIEEMLADPIRLNTNLGYYTNYINLLDYSAVAVPAGFQ
HHHHHHHHHCCEEECCCCCHHHHHHHHHCCCEEECCCCCHHHHHHHHHHCCCEECCCCCC
KDGLPFGVTLVAPAHQDGSLLHLAARLQQVYALPLGATDTPLEKEVLTEKGSFQRPPPPE
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCC
QPALVRVAVCGAHLSGLPLNPQLTSRNGRLVASTTTSPDYKFYALPGIPPLRPGLVRVDK
CCCEEEEEEECCHHCCCCCCCCEECCCCEEEEEECCCCCEEEEECCCCCCCCCCEEEECC
NERGAAIEVEVWELPASEFGSFVAGIPAPLGIGTITLAGGESVQGFLCEGYAVGDARDIS
CCCCCEEEEEEEECCHHHHHHHHHCCCCCCCCCEEEECCCCCCCCEEECCEECCCHHHHH
QFGGWRRYMQSTADPIASEEVVREVDPG
HHHHHHHHHHHHCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]