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The map label for this gene is yfcG [H]

Identifier: 82702020

GI number: 82702020

Start: 1012367

End: 1013092

Strand: Reverse

Name: yfcG [H]

Synonym: Nmul_A0891

Alternate gene names: 82702020

Gene position: 1013092-1012367 (Counterclockwise)

Preceding gene: 82702022

Following gene: 82702018

Centisome position: 31.82

GC content: 55.37

Gene sequence:

>726_bases
ATGATCGAACTGCATTCCAGTCCAACCCCGAACGGCCAGAAAGTGATGATCATGCTGGAAGAAATCGGAATCGAATGGAA
ACACATCGACGTCGATATCAATGTGGGGGAGCAATTCCAGCCGGAGCATCTGAAACTCAATCCCAATAACAAGATTCCGG
CGATCGTGGATATGGAGGGTCCGGGGGGTAGCCCCTACACAATGATGGAATCGGGTGCGATTCTCCTTTATCTCGCGGAG
AAAACAGGCAGATTCCTGCCTGTGGAGGCGCATAAGCGCTATGATACGCTGCAATGGCTGTTCTTCCAGGTGGCGCATAT
CGGCCCCATGTTCGGGCAGGCGAACCATTTCAATCGGCAGGCCAGAGACAACCAATACGCGCGGGACAGGTACAACAATG
AGTCGCTGCGCCTCTATCGAGTGCTGGAAAACCGGCTGGGCGCATCCGCGTGGCTCGGGTGCGATGAATATTCAATCGCC
GATATCGCCACCTACCCTTGGTGTAAAGGGCATGCGGAGCGCGGCGTCGGTGGAGAGGCCTTCCCGAGCTTCATGCGCTG
GTTTACTGCAATGGAGGCGCGTCCCGCAGTGCAGCGCAATAATGAAATGGCAGCCGGAATTCGGGCCAGAATGGAAAAGG
CGGCAAAGGATAAGCCTGTTATCAATATGTATGACACTAGGGATAATGCCGAGCGGCTTGCCCGGGCGACGTCGCACAAG
CCATGA

Upstream 100 bases:

>100_bases
ATGAGGCAACAAGACAAACCAGACAATGGAGGGACCATGCTCTGCCATCCACGAGTAATTGACGGAAAGAACCAATCATG
CAAGTGACCCAGGAGGAATC

Downstream 100 bases:

>100_bases
GAAAGGCAGGAGAAAGGCACGAGAAATTTATCGGGCGCTGCGCAATTTGCGGCCTGACGCTTGCCCCTTTCAGAGCAAGC
AGCTCAAACAGGCCAAGCGA

Product: glutathione S-transferase-like protein

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MIELHSSPTPNGQKVMIMLEEIGIEWKHIDVDINVGEQFQPEHLKLNPNNKIPAIVDMEGPGGSPYTMMESGAILLYLAE
KTGRFLPVEAHKRYDTLQWLFFQVAHIGPMFGQANHFNRQARDNQYARDRYNNESLRLYRVLENRLGASAWLGCDEYSIA
DIATYPWCKGHAERGVGGEAFPSFMRWFTAMEARPAVQRNNEMAAGIRARMEKAAKDKPVINMYDTRDNAERLARATSHK
P

Sequences:

>Translated_241_residues
MIELHSSPTPNGQKVMIMLEEIGIEWKHIDVDINVGEQFQPEHLKLNPNNKIPAIVDMEGPGGSPYTMMESGAILLYLAE
KTGRFLPVEAHKRYDTLQWLFFQVAHIGPMFGQANHFNRQARDNQYARDRYNNESLRLYRVLENRLGASAWLGCDEYSIA
DIATYPWCKGHAERGVGGEAFPSFMRWFTAMEARPAVQRNNEMAAGIRARMEKAAKDKPVINMYDTRDNAERLARATSHK
P
>Mature_241_residues
MIELHSSPTPNGQKVMIMLEEIGIEWKHIDVDINVGEQFQPEHLKLNPNNKIPAIVDMEGPGGSPYTMMESGAILLYLAE
KTGRFLPVEAHKRYDTLQWLFFQVAHIGPMFGQANHFNRQARDNQYARDRYNNESLRLYRVLENRLGASAWLGCDEYSIA
DIATYPWCKGHAERGVGGEAFPSFMRWFTAMEARPAVQRNNEMAAGIRARMEKAAKDKPVINMYDTRDNAERLARATSHK
P

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=199, Percent_Identity=49.748743718593, Blast_Score=195, Evalue=2e-51,
Organism=Escherichia coli, GI87082195, Length=212, Percent_Identity=38.6792452830189, Blast_Score=127, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6324100, Length=229, Percent_Identity=34.061135371179, Blast_Score=99, Evalue=5e-22,
Organism=Drosophila melanogaster, GI28571670, Length=196, Percent_Identity=26.0204081632653, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17864596, Length=216, Percent_Identity=26.8518518518519, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 27511; Mature: 27511

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIELHSSPTPNGQKVMIMLEEIGIEWKHIDVDINVGEQFQPEHLKLNPNNKIPAIVDMEG
CEECCCCCCCCCCEEEEEEHHHCCEEEEEEEEECCCCCCCCCEEEECCCCCCCEEEEECC
PGGSPYTMMESGAILLYLAEKTGRFLPVEAHKRYDTLQWLFFQVAHIGPMFGQANHFNRQ
CCCCCCEEECCCEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHCHHCCCCCCCCCH
ARDNQYARDRYNNESLRLYRVLENRLGASAWLGCDEYSIADIATYPWCKGHAERGVGGEA
HHCCHHHHHHCCCCHHHHHHHHHHHCCCCEECCCCCCCHHHHCCCCCCCCCCCCCCCHHH
FPSFMRWFTAMEARPAVQRNNEMAAGIRARMEKAAKDKPVINMYDTRDNAERLARATSHK
HHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCC
P
C
>Mature Secondary Structure
MIELHSSPTPNGQKVMIMLEEIGIEWKHIDVDINVGEQFQPEHLKLNPNNKIPAIVDMEG
CEECCCCCCCCCCEEEEEEHHHCCEEEEEEEEECCCCCCCCCEEEECCCCCCCEEEEECC
PGGSPYTMMESGAILLYLAEKTGRFLPVEAHKRYDTLQWLFFQVAHIGPMFGQANHFNRQ
CCCCCCEEECCCEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHCHHCCCCCCCCCH
ARDNQYARDRYNNESLRLYRVLENRLGASAWLGCDEYSIADIATYPWCKGHAERGVGGEA
HHCCHHHHHHCCCCHHHHHHHHHHHCCCCEECCCCCCCHHHHCCCCCCCCCCCCCCCHHH
FPSFMRWFTAMEARPAVQRNNEMAAGIRARMEKAAKDKPVINMYDTRDNAERLARATSHK
HHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCC
P
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]