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The map label for this gene is prpD [H]

Identifier: 82702000

GI number: 82702000

Start: 989317

End: 990768

Strand: Direct

Name: prpD [H]

Synonym: Nmul_A0871

Alternate gene names: 82702000

Gene position: 989317-990768 (Clockwise)

Preceding gene: 82701996

Following gene: 82702003

Centisome position: 31.07

GC content: 57.09

Gene sequence:

>1452_bases
ATGTCATCCGCGTCCTCCCACATTCGTCCCCAGCCTGACCAGGTTCTGGTCGACATTGCAGATTATGTGGTGGGACGGGA
TATTCAAAGCGATCTGGCTTACGCTACCGCCCGTCACTGCCTGATGGATTCCCTGGGGTGCGCCATGGAAGCGCTTGCGT
ATCCGGCCTGCACCAAGCTGCTTGGGCCGCTCGTGCCCGAGGCTGCCGAATCTGAGGGCGGCGCGAGGATTCCGGGTACC
CAGTTTCAGTTCAATCCGGTGGAAGCGGCATTCAATATCGGCACGATGATTCGCTGGCTCGATTTCAACGATACCTGGCT
GGCTGCGGAATGGGGTCATCCATCGGATAACCTGGGGGCCATACTGGCTGTAGCCGACTGGCTTTCGCGCAGTGCCACGA
GCGGGAGGCGGCGGCCGCTTATGCGCGATGTGTTGACCGCGATGATAAAGGCATACGAGATCCAGGGTTGCTTCGCTTTG
GAAAACAGCTTCAACAGGGTGGGCCTGGATCATGTGGTGCTGGTTAAAGTTGCCTCCACAGCCGTCGTAACGCATCTGCT
GGGCGGCAGCCGTAAACAGATCGTCGATGCGCTTTCCCAGGCGTGGGTGGACGGGCAGGCACTTCGCACGTATCGTCATG
CCCCCAACACCGGCTCGCGCAAATCCTGGGCTGCGGGGGATGCCACCAGTCGTGCGGTATGGCTTGCGCTGATCACACTC
AAGGGGGAGCCAGGCTATCCTTCAGCGCTTACCGCGAAAACCTGGGGGTTCTACGATGTGCTGTTCAAGGGAGAACCTTT
CAAATTCCAGAGGCCCATCGAACGATGGCATTCCTATGTAATGGAGAATGTGCTGCTCAAGATTTCGTTCCCCGCCGAAT
TCCACTCCCAGACAGCGGCGGAGTGTGCGATGCAGCTATACCCGCACGTGAAGGACCGGATTGCCGATATCCGGAAAATA
ACGATCCGCACCCACGAGGCCGCGATTCGTATCATCGACAAGAAGGGGCCGCTCAGCAGCCCGGCCGACCGCGACCATTG
CATGCAGTATATCGTGGCAGTGGCCCTTATTTTCGGCAGGCTTACCTCTGCCGATTATGAGGATGGCGTGGCCGCCGACC
CTCGCATAGATGCGTTGCGGGACAAGATAATCTGTATTGAGGATCCGTGCTTCACAAAGGATTATTACGATCCCGAAAAA
CGCTCCATCGCAAACGGTCTCACCCTTGAATTCAGGGACGGCAGTAAACTGGAGGAAGTGGTGGTGGAGTATCCCATCGG
CCACAGGTTGCGTCGAAGCGAAGGCATTCCGCTACTGGAAGAAAAATTTAGAATCAATCTTGGGCGGCGTTTCCCCGCCC
AGCAGTGCGAGGCAATCATGAATGCCTGTCATGATCAAGGCAGGCTGGAAGCAATGCCGGTTCACGAGTTTATCGATCTG
TTTGTGATTTAG

Upstream 100 bases:

>100_bases
GGTGTTTTTGCAGGAAAGACGGTGCCAAGAAGGAAAAACGGCTTCTTCTTTCGCCGCTCAGGCTCTCCTTGTTGGAGTGC
AGGCTCTATAATATGGGCCC

Downstream 100 bases:

>100_bases
GTGAGCTGGTTGCTGCTTCCGGATGTGTAACTTGAGGCGGGGTGAGTTCCCTTTCGGTGAAGTCGTGACCGCTCCCCTGC
CGGGAAGAGTTTGTATCCAG

Product: 2-methylcitrate dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MSSASSHIRPQPDQVLVDIADYVVGRDIQSDLAYATARHCLMDSLGCAMEALAYPACTKLLGPLVPEAAESEGGARIPGT
QFQFNPVEAAFNIGTMIRWLDFNDTWLAAEWGHPSDNLGAILAVADWLSRSATSGRRRPLMRDVLTAMIKAYEIQGCFAL
ENSFNRVGLDHVVLVKVASTAVVTHLLGGSRKQIVDALSQAWVDGQALRTYRHAPNTGSRKSWAAGDATSRAVWLALITL
KGEPGYPSALTAKTWGFYDVLFKGEPFKFQRPIERWHSYVMENVLLKISFPAEFHSQTAAECAMQLYPHVKDRIADIRKI
TIRTHEAAIRIIDKKGPLSSPADRDHCMQYIVAVALIFGRLTSADYEDGVAADPRIDALRDKIICIEDPCFTKDYYDPEK
RSIANGLTLEFRDGSKLEEVVVEYPIGHRLRRSEGIPLLEEKFRINLGRRFPAQQCEAIMNACHDQGRLEAMPVHEFIDL
FVI

Sequences:

>Translated_483_residues
MSSASSHIRPQPDQVLVDIADYVVGRDIQSDLAYATARHCLMDSLGCAMEALAYPACTKLLGPLVPEAAESEGGARIPGT
QFQFNPVEAAFNIGTMIRWLDFNDTWLAAEWGHPSDNLGAILAVADWLSRSATSGRRRPLMRDVLTAMIKAYEIQGCFAL
ENSFNRVGLDHVVLVKVASTAVVTHLLGGSRKQIVDALSQAWVDGQALRTYRHAPNTGSRKSWAAGDATSRAVWLALITL
KGEPGYPSALTAKTWGFYDVLFKGEPFKFQRPIERWHSYVMENVLLKISFPAEFHSQTAAECAMQLYPHVKDRIADIRKI
TIRTHEAAIRIIDKKGPLSSPADRDHCMQYIVAVALIFGRLTSADYEDGVAADPRIDALRDKIICIEDPCFTKDYYDPEK
RSIANGLTLEFRDGSKLEEVVVEYPIGHRLRRSEGIPLLEEKFRINLGRRFPAQQCEAIMNACHDQGRLEAMPVHEFIDL
FVI
>Mature_482_residues
SSASSHIRPQPDQVLVDIADYVVGRDIQSDLAYATARHCLMDSLGCAMEALAYPACTKLLGPLVPEAAESEGGARIPGTQ
FQFNPVEAAFNIGTMIRWLDFNDTWLAAEWGHPSDNLGAILAVADWLSRSATSGRRRPLMRDVLTAMIKAYEIQGCFALE
NSFNRVGLDHVVLVKVASTAVVTHLLGGSRKQIVDALSQAWVDGQALRTYRHAPNTGSRKSWAAGDATSRAVWLALITLK
GEPGYPSALTAKTWGFYDVLFKGEPFKFQRPIERWHSYVMENVLLKISFPAEFHSQTAAECAMQLYPHVKDRIADIRKIT
IRTHEAAIRIIDKKGPLSSPADRDHCMQYIVAVALIFGRLTSADYEDGVAADPRIDALRDKIICIEDPCFTKDYYDPEKR
SIANGLTLEFRDGSKLEEVVVEYPIGHRLRRSEGIPLLEEKFRINLGRRFPAQQCEAIMNACHDQGRLEAMPVHEFIDLF
VI

Specific function: Catalyzes the dehydration of 2-methylcitrate to 2- methyl-cis-aconitate [H]

COG id: COG2079

COG function: function code R; Uncharacterized protein involved in propionate catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prpD family [H]

Homologues:

Organism=Escherichia coli, GI1786528, Length=487, Percent_Identity=68.5831622176591, Blast_Score=696, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6325258, Length=491, Percent_Identity=56.4154786150713, Blast_Score=548, Evalue=1e-157,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012705
- InterPro:   IPR005656 [H]

Pfam domain/function: PF03972 MmgE_PrpD [H]

EC number: =4.2.1.79 [H]

Molecular weight: Translated: 53859; Mature: 53728

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSASSHIRPQPDQVLVDIADYVVGRDIQSDLAYATARHCLMDSLGCAMEALAYPACTKL
CCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGPLVPEAAESEGGARIPGTQFQFNPVEAAFNIGTMIRWLDFNDTWLAAEWGHPSDNLGA
HHHCCCCHHCCCCCCCCCCCCEECCHHHHHHHHCCEEEEEECCCCEEEEECCCCCCCHHH
ILAVADWLSRSATSGRRRPLMRDVLTAMIKAYEIQGCFALENSFNRVGLDHVVLVKVAST
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCEEEHHHHHH
AVVTHLLGGSRKQIVDALSQAWVDGQALRTYRHAPNTGSRKSWAAGDATSRAVWLALITL
HHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEE
KGEPGYPSALTAKTWGFYDVLFKGEPFKFQRPIERWHSYVMENVLLKISFPAEFHSQTAA
ECCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHH
ECAMQLYPHVKDRIADIRKITIRTHEAAIRIIDKKGPLSSPADRDHCMQYIVAVALIFGR
HHHHHHHHHHHHHHHHHHHHEEEECCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
LTSADYEDGVAADPRIDALRDKIICIEDPCFTKDYYDPEKRSIANGLTLEFRDGSKLEEV
HCCCCCCCCCCCCCCHHHHHCCEEEECCCCCCCCCCCCHHHHHHCCCEEEECCCCHHHHH
VVEYPIGHRLRRSEGIPLLEEKFRINLGRRFPAQQCEAIMNACHDQGRLEAMPVHEFIDL
HHHCCCCHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEECHHHHHHH
FVI
HCC
>Mature Secondary Structure 
SSASSHIRPQPDQVLVDIADYVVGRDIQSDLAYATARHCLMDSLGCAMEALAYPACTKL
CCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGPLVPEAAESEGGARIPGTQFQFNPVEAAFNIGTMIRWLDFNDTWLAAEWGHPSDNLGA
HHHCCCCHHCCCCCCCCCCCCEECCHHHHHHHHCCEEEEEECCCCEEEEECCCCCCCHHH
ILAVADWLSRSATSGRRRPLMRDVLTAMIKAYEIQGCFALENSFNRVGLDHVVLVKVAST
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCEEEHHHHHH
AVVTHLLGGSRKQIVDALSQAWVDGQALRTYRHAPNTGSRKSWAAGDATSRAVWLALITL
HHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEE
KGEPGYPSALTAKTWGFYDVLFKGEPFKFQRPIERWHSYVMENVLLKISFPAEFHSQTAA
ECCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHH
ECAMQLYPHVKDRIADIRKITIRTHEAAIRIIDKKGPLSSPADRDHCMQYIVAVALIFGR
HHHHHHHHHHHHHHHHHHHHEEEECCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
LTSADYEDGVAADPRIDALRDKIICIEDPCFTKDYYDPEKRSIANGLTLEFRDGSKLEEV
HCCCCCCCCCCCCCCHHHHHCCEEEECCCCCCCCCCCCHHHHHHCCCEEEECCCCHHHHH
VVEYPIGHRLRRSEGIPLLEEKFRINLGRRFPAQQCEAIMNACHDQGRLEAMPVHEFIDL
HHHCCCCHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEECHHHHHHH
FVI
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]