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The map label for this gene is rpoA

Identifier: 82701925

GI number: 82701925

Start: 908158

End: 909162

Strand: Direct

Name: rpoA

Synonym: Nmul_A0792

Alternate gene names: 82701925

Gene position: 908158-909162 (Clockwise)

Preceding gene: 82701924

Following gene: 82701926

Centisome position: 28.52

GC content: 53.23

Gene sequence:

>1005_bases
ATGCAAAGTAACACCTTTCTTACGCCGCGTATTATCGATGTGCAGAATATATCGCCGCTCCATGCAAGAATCACGATGGA
GCCGTTCGAGCGCGGCTATGGCCATACGCTGGGCAACGCGTTACGGCGTATTCTGCTTTCATCGATGCCGGGATTTGCGC
CAACGGAAGTTCAGATCAGTGGTGTCGTTCACGAATACTCCGCCCTGGACGGCGTGCAGGAAGACGTGGTTGATATCCTG
CTCAATCTCAAAGGTATTGTCCTGAAATTGCACAATCGTACAGAGGCACTTCTGACCCTGAGCAAAAAGGGAGAAGGAAC
GGTTACTGCGGGCGATATCGAGGGGGGGCATGATGTCGAGATCATTAATCCCGACCATGTCGTTGCCCACCTTACGTCCG
GCGGCAAGCTGGATATGCAGATCAAGGTGGAAATGGGACGGGGCTACGTGCCGGGAACTGTCCGCCAGTTGGCGCACGAA
AGCCGTGCTATCGGCAGCATTCTGCTGGATGCATCTTTCAGTCCTGTGCGGCGGGTAAGTTATGCAGTGGAAAGCGCCCG
GGTTGAACAACGCACCGATCTGGATAAACTGGTCATGGACATCGAGACCAATGGTGTCGTTGACCCCGAGGAAGCAATCC
GGTATGCGGCAAAAGTCCTGGTCGAGCAACTCTCCGTATTTGCGGACCTCAAAGGCACGCCGCTGCCCGTGGAACAACCG
AAATTGCCCCAGATTGATCCAGTGCTGCTTCGGCCGGTGGATGATCTTGAACTCACCGTACGTTCCGCGAATTGTCTCAA
AGCGGAAAATATATATTATATTGGCGACCTGATCCAGCGGACGGAAACCGAGCTACTGAAAACCCCTAATCTGGGGCGGA
AATCCTTGAATGAAATCAAGGAAGTGCTTGCTTCTCGCGGGTTGACGCTGGGCATGAAACTGGAGAGCTGGCCACCTTCC
AATCTTGATAAGGGTAAAAAGGATACAAGTCATGCGGCACCATAA

Upstream 100 bases:

>100_bases
ACCCGAGAGATCCGATTTACCGTCAAGCATCAATGAATCTCTCGTAATTGAATTGTATTCCAAGTAATGAGTCGCCACGT
TTCCGCAAAAGGATCAGGCT

Downstream 100 bases:

>100_bases
TGGACTAAGGAAGCTTAATCGCACGAGCAGTCACCGCTCGGCCATGTTACGGAACATGACCAACTCCCTGTTGCGGCACG
AGGTTATAAAAACGACTTTG

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MQSNTFLTPRIIDVQNISPLHARITMEPFERGYGHTLGNALRRILLSSMPGFAPTEVQISGVVHEYSALDGVQEDVVDIL
LNLKGIVLKLHNRTEALLTLSKKGEGTVTAGDIEGGHDVEIINPDHVVAHLTSGGKLDMQIKVEMGRGYVPGTVRQLAHE
SRAIGSILLDASFSPVRRVSYAVESARVEQRTDLDKLVMDIETNGVVDPEEAIRYAAKVLVEQLSVFADLKGTPLPVEQP
KLPQIDPVLLRPVDDLELTVRSANCLKAENIYYIGDLIQRTETELLKTPNLGRKSLNEIKEVLASRGLTLGMKLESWPPS
NLDKGKKDTSHAAP

Sequences:

>Translated_334_residues
MQSNTFLTPRIIDVQNISPLHARITMEPFERGYGHTLGNALRRILLSSMPGFAPTEVQISGVVHEYSALDGVQEDVVDIL
LNLKGIVLKLHNRTEALLTLSKKGEGTVTAGDIEGGHDVEIINPDHVVAHLTSGGKLDMQIKVEMGRGYVPGTVRQLAHE
SRAIGSILLDASFSPVRRVSYAVESARVEQRTDLDKLVMDIETNGVVDPEEAIRYAAKVLVEQLSVFADLKGTPLPVEQP
KLPQIDPVLLRPVDDLELTVRSANCLKAENIYYIGDLIQRTETELLKTPNLGRKSLNEIKEVLASRGLTLGMKLESWPPS
NLDKGKKDTSHAAP
>Mature_334_residues
MQSNTFLTPRIIDVQNISPLHARITMEPFERGYGHTLGNALRRILLSSMPGFAPTEVQISGVVHEYSALDGVQEDVVDIL
LNLKGIVLKLHNRTEALLTLSKKGEGTVTAGDIEGGHDVEIINPDHVVAHLTSGGKLDMQIKVEMGRGYVPGTVRQLAHE
SRAIGSILLDASFSPVRRVSYAVESARVEQRTDLDKLVMDIETNGVVDPEEAIRYAAKVLVEQLSVFADLKGTPLPVEQP
KLPQIDPVLLRPVDDLELTVRSANCLKAENIYYIGDLIQRTETELLKTPNLGRKSLNEIKEVLASRGLTLGMKLESWPPS
NLDKGKKDTSHAAP

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=319, Percent_Identity=62.6959247648903, Blast_Score=420, Evalue=1e-119,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_NITMU (Q2YAX2)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_411491.1
- HSSP:   P20429
- ProteinModelPortal:   Q2YAX2
- STRING:   Q2YAX2
- GeneID:   3785245
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0792
- eggNOG:   COG0202
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- PhylomeDB:   Q2YAX2
- ProtClustDB:   PRK05182
- BioCyc:   NMUL323848:NMUL_A0792-MONOMER
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 36640; Mature: 36640

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSNTFLTPRIIDVQNISPLHARITMEPFERGYGHTLGNALRRILLSSMPGFAPTEVQIS
CCCCCCCCCEEEECCCCCCCEEEEEECHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEEE
GVVHEYSALDGVQEDVVDILLNLKGIVLKLHNRTEALLTLSKKGEGTVTAGDIEGGHDVE
HHHHHHHHHCCHHHHHHHHHHCCCEEEEEECCCCEEEEEEECCCCCCEEECCCCCCCEEE
IINPDHVVAHLTSGGKLDMQIKVEMGRGYVPGTVRQLAHESRAIGSILLDASFSPVRRVS
EECCCEEEEEECCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHEECCCCCHHHHHH
YAVESARVEQRTDLDKLVMDIETNGVVDPEEAIRYAAKVLVEQLSVFADLKGTPLPVEQP
HHHHHHHHHHHCCHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
KLPQIDPVLLRPVDDLELTVRSANCLKAENIYYIGDLIQRTETELLKTPNLGRKSLNEIK
CCCCCCCEEECCCCCCEEEEECCCCEECCCEEEHHHHHHHHHHHHHCCCCCCHHHHHHHH
EVLASRGLTLGMKLESWPPSNLDKGKKDTSHAAP
HHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQSNTFLTPRIIDVQNISPLHARITMEPFERGYGHTLGNALRRILLSSMPGFAPTEVQIS
CCCCCCCCCEEEECCCCCCCEEEEEECHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEEE
GVVHEYSALDGVQEDVVDILLNLKGIVLKLHNRTEALLTLSKKGEGTVTAGDIEGGHDVE
HHHHHHHHHCCHHHHHHHHHHCCCEEEEEECCCCEEEEEEECCCCCCEEECCCCCCCEEE
IINPDHVVAHLTSGGKLDMQIKVEMGRGYVPGTVRQLAHESRAIGSILLDASFSPVRRVS
EECCCEEEEEECCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHEECCCCCHHHHHH
YAVESARVEQRTDLDKLVMDIETNGVVDPEEAIRYAAKVLVEQLSVFADLKGTPLPVEQP
HHHHHHHHHHHCCHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
KLPQIDPVLLRPVDDLELTVRSANCLKAENIYYIGDLIQRTETELLKTPNLGRKSLNEIK
CCCCCCCEEECCCCCCEEEEECCCCEECCCEEEHHHHHHHHHHHHHCCCCCCHHHHHHHH
EVLASRGLTLGMKLESWPPSNLDKGKKDTSHAAP
HHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA