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The map label for this gene is ligA

Identifier: 82701780

GI number: 82701780

Start: 745040

End: 747163

Strand: Direct

Name: ligA

Synonym: Nmul_A0647

Alternate gene names: 82701780

Gene position: 745040-747163 (Clockwise)

Preceding gene: 82701779

Following gene: 82701781

Centisome position: 23.4

GC content: 55.37

Gene sequence:

>2124_bases
GTGAGTGAACGCCTGAGCGAGATATCGCGCCACATGCGGGAATTGCGCGATGCAATCGAGCTGCATAATATCCGTTATTA
CGAGCTGGACACTCCGACGATTCCCGATGCGGACTTCGACAAGCTGTTCCGCGAACTGGAGGAGCTCGAGCAACGTCATC
CGGAGCTCATAACGCCAGATTCCCCTACCCAGCGCGTTGGTATTCCTCCCCTCAAAGCGTTTCCCCAGGTCGTTCACCGT
ACGCCGATGCTTTCCCTCAGCAATGCATTCACCGAGGGCGAGGTAGAGGCATTCGACCGGCGTGTGCGCCAGTCCCTAGG
CATCGCGCAGGTCGAGTATGCGGTAGAGCCGAAATTCGATGGTCTGGCCATAAGCCTCTGCTATGAGAACGGCATGCTTG
TCTCGGGAGCCACTCGCGGAGATGGCTACATCGGAGAGGATGTAACCTTGAACCTGAAAACCATTCGCAGCATTCCTCTC
GGCCTGAGGGCAGGAGATGGGAGCGCCGAAGTTCCTGCATTTCTGGAGGTCCGGGGTGAGGTATTAATGTTGAAAGCGGA
TTTCGAGCGATTGAATCGGCAGCAGCGTGAAAAGAACGAAAAGACCTTTATAAATCCGCGCAATGCTGCCGCAGGTTCCT
TGCGGCAACTCGATCCCTGCATAACTGCCAATCGCAACCTGAGGTTCTTCGCATATGGTATCGGTGTCTGCGAAGGGGGA
AATGTTCCTCATGACACACACAGCCACTTGCTCGACTATCTCTCGTCACTGCGTTTTCCGGTAATGCGTGAACGCAGGAC
GGTGACGGGCGTTGCCGGTTTGTTGGAGTATCATCATGAAATAAATGGATTGCGCGAACAATTGTCTTATGACATCGATG
GTACGGTGTACAAGGTCAACGAATTGGAATATCAGGAAAAACTCGGTTTCGTATCCCGTGCCCCGCGATTTGCGCTCGCC
CATAAGTTTCCAGCGCAGGAAGCAACGACCGAGTTGCTTGGAATCGATGTGCAGGTGGGCAGAACGGGGGCGCTGACTCC
GGTGGCGCGCCTCAAGCCTGTATTCGTGGGAGGCGCGACTGTCACCAATGCGACCCTGCATAACGAAGATGAGATCCGGC
GCAAGGACGTCATGATCGGTGACCGGGTAACGGTAAGGCGAGCAGGAGATGTCATTCCGGAAGTGGTCGCAGTTCAGAAG
GAAAGCCGGCCGTCGAACGCGAAACCTTTCGTGATGCCTGAGCACTGCCCTGTTTGCGGGTCCAGGACGATTCGCTTGCC
TGGAGAAACTCTGACCCGCTGCACGGGCGGATTGTTTTGTCCCGCTCAACGGAAACAGGCCATTCTGCATTTTGCCTCGC
GTCGCGCCATGAATATCCACGGACTGGGCGACAAGCTGGTGGATCACCTGGTGGACAATGCCATCATTCGGACTCCCGCA
GACTTGTATAAACTGGGATTGGCAGCGCTCGCCGCGCTGGATCGGATGGGGGAGAAATCGGCGGGAAATATTATCAATGA
AATTGAAAAAAGCAAAGACACGACCCTGGCTCGATTCATCTATTCCCTGGGTATTCGCAACGTGGGCGAGGCTACTGCGC
GAGACTTGGCCCGCTATTTCGGAGGACTCGATCGCCTGATGGATGCAGATGTGGAAACTTTGCAACAGGTATCGGATGTA
GGCCCTGTCGTCGCCCAGAGCATTGCCGATTTTTTTGGTGAAAGGCACAATCGTGAGGTTGTAGAGCAATTGCGCGCGGC
GGGAATCCGCTGGAAAGAAGGTGCGGGAACACAGACAACAGACCATGCAGCAGGTTCTGCACATCCGGGAGCAGCCGCCG
CAAGCAGTAAAATTGCCGGCAGGAGCTTTGTGCTGACTGGAACACTGCCGACCATGAGCCGGGAGGAGGCCAGGGAGAAA
ATTGAAGCGATGGGAGGGAAGGTTGCGGGCAGCGTGTCCAAGAAAACCGATTATGTCGTCGCCGGCGCGGATGCGGGCAG
CAAGTATGACAAAGCGCTTGAATTGGGAGTGGCGCTTCTCGATGAGGCCGAGTTGATAAGATTATTGCAGGCTTCCCAAG
CAGATAACAGTTCGACGCACGTGGATCCTGAACGGATGGTTTAA

Upstream 100 bases:

>100_bases
CCGGTATCCGGAAAATCAAGCAACAATTGAGCAGCATTCAATCCGTGATGATATCGCGCAATATCCCGCCGGGGGGGCAG
ATAGCGTTGAGGCTGTTTGC

Downstream 100 bases:

>100_bases
TGCCGAAAATACGTAAAGCAGTTTTTCCGGTGGCAGGAATGGGCACCCGTTTTCTCCCTGCTACGAAGGCAAATCCCAAG
GAAATGATGCCTGTTGTAGA

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 707; Mature: 706

Protein sequence:

>707_residues
MSERLSEISRHMRELRDAIELHNIRYYELDTPTIPDADFDKLFRELEELEQRHPELITPDSPTQRVGIPPLKAFPQVVHR
TPMLSLSNAFTEGEVEAFDRRVRQSLGIAQVEYAVEPKFDGLAISLCYENGMLVSGATRGDGYIGEDVTLNLKTIRSIPL
GLRAGDGSAEVPAFLEVRGEVLMLKADFERLNRQQREKNEKTFINPRNAAAGSLRQLDPCITANRNLRFFAYGIGVCEGG
NVPHDTHSHLLDYLSSLRFPVMRERRTVTGVAGLLEYHHEINGLREQLSYDIDGTVYKVNELEYQEKLGFVSRAPRFALA
HKFPAQEATTELLGIDVQVGRTGALTPVARLKPVFVGGATVTNATLHNEDEIRRKDVMIGDRVTVRRAGDVIPEVVAVQK
ESRPSNAKPFVMPEHCPVCGSRTIRLPGETLTRCTGGLFCPAQRKQAILHFASRRAMNIHGLGDKLVDHLVDNAIIRTPA
DLYKLGLAALAALDRMGEKSAGNIINEIEKSKDTTLARFIYSLGIRNVGEATARDLARYFGGLDRLMDADVETLQQVSDV
GPVVAQSIADFFGERHNREVVEQLRAAGIRWKEGAGTQTTDHAAGSAHPGAAAASSKIAGRSFVLTGTLPTMSREEAREK
IEAMGGKVAGSVSKKTDYVVAGADAGSKYDKALELGVALLDEAELIRLLQASQADNSSTHVDPERMV

Sequences:

>Translated_707_residues
MSERLSEISRHMRELRDAIELHNIRYYELDTPTIPDADFDKLFRELEELEQRHPELITPDSPTQRVGIPPLKAFPQVVHR
TPMLSLSNAFTEGEVEAFDRRVRQSLGIAQVEYAVEPKFDGLAISLCYENGMLVSGATRGDGYIGEDVTLNLKTIRSIPL
GLRAGDGSAEVPAFLEVRGEVLMLKADFERLNRQQREKNEKTFINPRNAAAGSLRQLDPCITANRNLRFFAYGIGVCEGG
NVPHDTHSHLLDYLSSLRFPVMRERRTVTGVAGLLEYHHEINGLREQLSYDIDGTVYKVNELEYQEKLGFVSRAPRFALA
HKFPAQEATTELLGIDVQVGRTGALTPVARLKPVFVGGATVTNATLHNEDEIRRKDVMIGDRVTVRRAGDVIPEVVAVQK
ESRPSNAKPFVMPEHCPVCGSRTIRLPGETLTRCTGGLFCPAQRKQAILHFASRRAMNIHGLGDKLVDHLVDNAIIRTPA
DLYKLGLAALAALDRMGEKSAGNIINEIEKSKDTTLARFIYSLGIRNVGEATARDLARYFGGLDRLMDADVETLQQVSDV
GPVVAQSIADFFGERHNREVVEQLRAAGIRWKEGAGTQTTDHAAGSAHPGAAAASSKIAGRSFVLTGTLPTMSREEAREK
IEAMGGKVAGSVSKKTDYVVAGADAGSKYDKALELGVALLDEAELIRLLQASQADNSSTHVDPERMV
>Mature_706_residues
SERLSEISRHMRELRDAIELHNIRYYELDTPTIPDADFDKLFRELEELEQRHPELITPDSPTQRVGIPPLKAFPQVVHRT
PMLSLSNAFTEGEVEAFDRRVRQSLGIAQVEYAVEPKFDGLAISLCYENGMLVSGATRGDGYIGEDVTLNLKTIRSIPLG
LRAGDGSAEVPAFLEVRGEVLMLKADFERLNRQQREKNEKTFINPRNAAAGSLRQLDPCITANRNLRFFAYGIGVCEGGN
VPHDTHSHLLDYLSSLRFPVMRERRTVTGVAGLLEYHHEINGLREQLSYDIDGTVYKVNELEYQEKLGFVSRAPRFALAH
KFPAQEATTELLGIDVQVGRTGALTPVARLKPVFVGGATVTNATLHNEDEIRRKDVMIGDRVTVRRAGDVIPEVVAVQKE
SRPSNAKPFVMPEHCPVCGSRTIRLPGETLTRCTGGLFCPAQRKQAILHFASRRAMNIHGLGDKLVDHLVDNAIIRTPAD
LYKLGLAALAALDRMGEKSAGNIINEIEKSKDTTLARFIYSLGIRNVGEATARDLARYFGGLDRLMDADVETLQQVSDVG
PVVAQSIADFFGERHNREVVEQLRAAGIRWKEGAGTQTTDHAAGSAHPGAAAASSKIAGRSFVLTGTLPTMSREEAREKI
EAMGGKVAGSVSKKTDYVVAGADAGSKYDKALELGVALLDEAELIRLLQASQADNSSTHVDPERMV

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Homo sapiens, GI32528306, Length=74, Percent_Identity=50, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1788750, Length=686, Percent_Identity=52.6239067055394, Blast_Score=704, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_NITMU (Q2YBB7)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_411346.1
- ProteinModelPortal:   Q2YBB7
- STRING:   Q2YBB7
- GeneID:   3785420
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0647
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- PhylomeDB:   Q2YBB7
- BioCyc:   NMUL323848:NMUL_A0647-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 77730; Mature: 77599

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 118-118 BINDING 116-116 BINDING 139-139 BINDING 180-180 BINDING 298-298 BINDING 322-322

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERLSEISRHMRELRDAIELHNIRYYELDTPTIPDADFDKLFRELEELEQRHPELITPD
CCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEECCC
SPTQRVGIPPLKAFPQVVHRTPMLSLSNAFTEGEVEAFDRRVRQSLGIAQVEYAVEPKFD
CCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCC
GLAISLCYENGMLVSGATRGDGYIGEDVTLNLKTIRSIPLGLRAGDGSAEVPAFLEVRGE
CEEEEEEECCCEEEECCCCCCCCCCCCCEEEHHHHHCCCCEEECCCCCCCCCHHEEECCC
VLMLKADFERLNRQQREKNEKTFINPRNAAAGSLRQLDPCITANRNLRFFAYGIGVCEGG
EEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCHHHCCCCCEEEEEEEEEEECCC
NVPHDTHSHLLDYLSSLRFPVMRERRTVTGVAGLLEYHHEINGLREQLSYDIDGTVYKVN
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEH
ELEYQEKLGFVSRAPRFALAHKFPAQEATTELLGIDVQVGRTGALTPVARLKPVFVGGAT
HHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHEEEEEECCCCCCCHHHHCCEEEECCCE
VTNATLHNEDEIRRKDVMIGDRVTVRRAGDVIPEVVAVQKESRPSNAKPFVMPEHCPVCG
EECCCCCCHHHHHHHHCEECCCEEEHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCC
SRTIRLPGETLTRCTGGLFCPAQRKQAILHFASRRAMNIHGLGDKLVDHLVDNAIIRTPA
CCEEECCCHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEECCCHHHHHHHHHCCHHHCCCH
DLYKLGLAALAALDRMGEKSAGNIINEIEKSKDTTLARFIYSLGIRNVGEATARDLARYF
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHH
GGLDRLMDADVETLQQVSDVGPVVAQSIADFFGERHNREVVEQLRAAGIRWKEGAGTQTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEECCCCCCCCC
DHAAGSAHPGAAAASSKIAGRSFVLTGTLPTMSREEAREKIEAMGGKVAGSVSKKTDYVV
CCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCHHCCCCCCCCCEEE
AGADAGSKYDKALELGVALLDEAELIRLLQASQADNSSTHVDPERMV
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCC
>Mature Secondary Structure 
SERLSEISRHMRELRDAIELHNIRYYELDTPTIPDADFDKLFRELEELEQRHPELITPD
CHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEECCC
SPTQRVGIPPLKAFPQVVHRTPMLSLSNAFTEGEVEAFDRRVRQSLGIAQVEYAVEPKFD
CCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCC
GLAISLCYENGMLVSGATRGDGYIGEDVTLNLKTIRSIPLGLRAGDGSAEVPAFLEVRGE
CEEEEEEECCCEEEECCCCCCCCCCCCCEEEHHHHHCCCCEEECCCCCCCCCHHEEECCC
VLMLKADFERLNRQQREKNEKTFINPRNAAAGSLRQLDPCITANRNLRFFAYGIGVCEGG
EEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCHHHCCCCCEEEEEEEEEEECCC
NVPHDTHSHLLDYLSSLRFPVMRERRTVTGVAGLLEYHHEINGLREQLSYDIDGTVYKVN
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEH
ELEYQEKLGFVSRAPRFALAHKFPAQEATTELLGIDVQVGRTGALTPVARLKPVFVGGAT
HHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHEEEEEECCCCCCCHHHHCCEEEECCCE
VTNATLHNEDEIRRKDVMIGDRVTVRRAGDVIPEVVAVQKESRPSNAKPFVMPEHCPVCG
EECCCCCCHHHHHHHHCEECCCEEEHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCC
SRTIRLPGETLTRCTGGLFCPAQRKQAILHFASRRAMNIHGLGDKLVDHLVDNAIIRTPA
CCEEECCCHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEECCCHHHHHHHHHCCHHHCCCH
DLYKLGLAALAALDRMGEKSAGNIINEIEKSKDTTLARFIYSLGIRNVGEATARDLARYF
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHH
GGLDRLMDADVETLQQVSDVGPVVAQSIADFFGERHNREVVEQLRAAGIRWKEGAGTQTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEECCCCCCCCC
DHAAGSAHPGAAAASSKIAGRSFVLTGTLPTMSREEAREKIEAMGGKVAGSVSKKTDYVV
CCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCHHCCCCCCCCCEEE
AGADAGSKYDKALELGVALLDEAELIRLLQASQADNSSTHVDPERMV
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA