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The map label for this gene is pheS

Identifier: 82701625

GI number: 82701625

Start: 549472

End: 550506

Strand: Direct

Name: pheS

Synonym: Nmul_A0491

Alternate gene names: 82701625

Gene position: 549472-550506 (Clockwise)

Preceding gene: 82701624

Following gene: 82701626

Centisome position: 17.26

GC content: 52.27

Gene sequence:

>1035_bases
ATGACTCATCTGGATCATCTTCTTACTGAAGCAATTGAGCTGTTCAATAGAACAGAGGACCTGGCCGAACTGGAGCAGGT
CAAGGCGCGATATCTGGGCCGGCATGGGCAACTGACCGAGTTGCTCAAGGGATTGGGCAAGATTCCCCCGGAAGAGCGTC
CCGCAGCGGGAAGCCGTATCAATCAGGCGAAGGAATCGCTGGAGGCAGCGCTGAGCCGTCGCCGGGAGGCGATACAGGAA
AAGAAGCTGGAAGAGCAGTTAATAGAGGAAAAGCTGGATGTCACTTTGTCGGGGCGCGGAACGGGAATGGGGGGACTCCA
CCCTATCACGCTCGCGCTCGAGCGAATTCAGTCGCTGTTTCATTCGGTCGGATTCACGGTGGCTTCCGGGCCCGAGATCG
AAACCGATTTTTATAATTTCACCGCGCTGAATATCCCGGAAAGCCATCCCGCTCGCGCCATGCACGATACTTTCTATGTC
GATGACGAAAACGGCGGCGCAAGCGAGCACCTGTTGCGAACTCATACGTCACCCGTTCAAATACGATACATGGAAAGTAA
TCCACCTCCCCTGAAGGTGATTGCGCCAGGGAGGGTTTACCGTTGCGATTCCGATCTGACGCATACTCCGATGTTTCATC
AGGTGGAAGGTTTATGGATCGATGAAAGTGCCAACTTCTCGGCTTTGAAAGGCATTCTCTCTGATTTCATGCAGCATTTT
TTCGAGCGCGACGATCTGCCGGTGCGTTTTCGCCCTTCTTTTTTCCCTTTTACCGAGCCTTCAGCCGAAATGGATATCGG
TTGTGTAATGTGCAAAACCGGCTGTCGCGTATGCAGTTACACCGGGTGGCTGGAAGTGCTGGGTTGTGGAATGGTACATC
CCAATGTCTTCAAGCATGTCAACATAGACAGTCAAAAGTACGTTGGCTTTGCTTTTGGTTTAGGCGTCGAGCGTCTGGCG
ATGCTGAGATATGGGGTAAACGACTTGCGACTGTTCTTCGAAAATGATTTGCGGTTCCTGAAACAGTTCAACTGA

Upstream 100 bases:

>100_bases
GCGCGTTTCCGGGATTAAAAGACTGGGAGGAGGCTAAAGGCAAAAACGCCTGGCCTCCTCTTTTCATATGCGGAATCCAA
TTGATCAAACTGTTATTTGA

Downstream 100 bases:

>100_bases
AACCACGGATCAGGAATATAAAAAGAAAAAATATAAAAAATCCCACGAAAAATAATGCAGGTGACATGCAACGGGATTCG
GGGCCCTTAAGGGTGACTGC

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MTHLDHLLTEAIELFNRTEDLAELEQVKARYLGRHGQLTELLKGLGKIPPEERPAAGSRINQAKESLEAALSRRREAIQE
KKLEEQLIEEKLDVTLSGRGTGMGGLHPITLALERIQSLFHSVGFTVASGPEIETDFYNFTALNIPESHPARAMHDTFYV
DDENGGASEHLLRTHTSPVQIRYMESNPPPLKVIAPGRVYRCDSDLTHTPMFHQVEGLWIDESANFSALKGILSDFMQHF
FERDDLPVRFRPSFFPFTEPSAEMDIGCVMCKTGCRVCSYTGWLEVLGCGMVHPNVFKHVNIDSQKYVGFAFGLGVERLA
MLRYGVNDLRLFFENDLRFLKQFN

Sequences:

>Translated_344_residues
MTHLDHLLTEAIELFNRTEDLAELEQVKARYLGRHGQLTELLKGLGKIPPEERPAAGSRINQAKESLEAALSRRREAIQE
KKLEEQLIEEKLDVTLSGRGTGMGGLHPITLALERIQSLFHSVGFTVASGPEIETDFYNFTALNIPESHPARAMHDTFYV
DDENGGASEHLLRTHTSPVQIRYMESNPPPLKVIAPGRVYRCDSDLTHTPMFHQVEGLWIDESANFSALKGILSDFMQHF
FERDDLPVRFRPSFFPFTEPSAEMDIGCVMCKTGCRVCSYTGWLEVLGCGMVHPNVFKHVNIDSQKYVGFAFGLGVERLA
MLRYGVNDLRLFFENDLRFLKQFN
>Mature_343_residues
THLDHLLTEAIELFNRTEDLAELEQVKARYLGRHGQLTELLKGLGKIPPEERPAAGSRINQAKESLEAALSRRREAIQEK
KLEEQLIEEKLDVTLSGRGTGMGGLHPITLALERIQSLFHSVGFTVASGPEIETDFYNFTALNIPESHPARAMHDTFYVD
DENGGASEHLLRTHTSPVQIRYMESNPPPLKVIAPGRVYRCDSDLTHTPMFHQVEGLWIDESANFSALKGILSDFMQHFF
ERDDLPVRFRPSFFPFTEPSAEMDIGCVMCKTGCRVCSYTGWLEVLGCGMVHPNVFKHVNIDSQKYVGFAFGLGVERLAM
LRYGVNDLRLFFENDLRFLKQFN

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5729820, Length=295, Percent_Identity=33.2203389830508, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI4758340, Length=271, Percent_Identity=30.6273062730627, Blast_Score=112, Evalue=4e-25,
Organism=Escherichia coli, GI1788007, Length=343, Percent_Identity=56.268221574344, Blast_Score=389, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI32566635, Length=290, Percent_Identity=33.1034482758621, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17508957, Length=269, Percent_Identity=30.1115241635688, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI32563657, Length=269, Percent_Identity=30.1115241635688, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6321087, Length=273, Percent_Identity=30.4029304029304, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6325304, Length=101, Percent_Identity=34.6534653465347, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI18858079, Length=268, Percent_Identity=28.3582089552239, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI17137424, Length=295, Percent_Identity=29.1525423728814, Blast_Score=99, Evalue=5e-21,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_NITMU (Q2YBS2)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_411191.1
- ProteinModelPortal:   Q2YBS2
- SMR:   Q2YBS2
- STRING:   Q2YBS2
- GeneID:   3784853
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0491
- eggNOG:   COG0016
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- PhylomeDB:   Q2YBS2
- ProtClustDB:   PRK00488
- BioCyc:   NMUL323848:NMUL_A0491-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 38974; Mature: 38843

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHLDHLLTEAIELFNRTEDLAELEQVKARYLGRHGQLTELLKGLGKIPPEERPAAGSRI
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCHHH
NQAKESLEAALSRRREAIQEKKLEEQLIEEKLDVTLSGRGTGMGGLHPITLALERIQSLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCCHHHHHHHHHHHHHH
HSVGFTVASGPEIETDFYNFTALNIPESHPARAMHDTFYVDDENGGASEHLLRTHTSPVQ
HHHCCEECCCCCCCCCCEEEEEECCCCCCCCHHHHCEEEEECCCCCHHHHHHHCCCCCEE
IRYMESNPPPLKVIAPGRVYRCDSDLTHTPMFHQVEGLWIDESANFSALKGILSDFMQHF
EEEECCCCCCEEEECCCEEEECCCCCCCCCCHHHHCCEEEECCCCHHHHHHHHHHHHHHH
FERDDLPVRFRPSFFPFTEPSAEMDIGCVMCKTGCRVCSYTGWLEVLGCGMVHPNVFKHV
HCCCCCCEEECCCCCCCCCCCCCCCHHEEEECCCCCHHHHCCHHHHHHCCCCCCCHHHCC
NIDSQKYVGFAFGLGVERLAMLRYGVNDLRLFFENDLRFLKQFN
CCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
THLDHLLTEAIELFNRTEDLAELEQVKARYLGRHGQLTELLKGLGKIPPEERPAAGSRI
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCHHH
NQAKESLEAALSRRREAIQEKKLEEQLIEEKLDVTLSGRGTGMGGLHPITLALERIQSLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCCHHHHHHHHHHHHHH
HSVGFTVASGPEIETDFYNFTALNIPESHPARAMHDTFYVDDENGGASEHLLRTHTSPVQ
HHHCCEECCCCCCCCCCEEEEEECCCCCCCCHHHHCEEEEECCCCCHHHHHHHCCCCCEE
IRYMESNPPPLKVIAPGRVYRCDSDLTHTPMFHQVEGLWIDESANFSALKGILSDFMQHF
EEEECCCCCCEEEECCCEEEECCCCCCCCCCHHHHCCEEEECCCCHHHHHHHHHHHHHHH
FERDDLPVRFRPSFFPFTEPSAEMDIGCVMCKTGCRVCSYTGWLEVLGCGMVHPNVFKHV
HCCCCCCEEECCCCCCCCCCCCCCCHHEEEECCCCCHHHHCCHHHHHHCCCCCCCHHHCC
NIDSQKYVGFAFGLGVERLAMLRYGVNDLRLFFENDLRFLKQFN
CCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA