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The map label for this gene is rlmE

Identifier: 82701616

GI number: 82701616

Start: 539375

End: 539995

Strand: Direct

Name: rlmE

Synonym: Nmul_A0482

Alternate gene names: 82701616

Gene position: 539375-539995 (Clockwise)

Preceding gene: 82701614

Following gene: 82701617

Centisome position: 16.94

GC content: 53.14

Gene sequence:

>621_bases
ATGAAGCGAACCAAAACCAGCAAGGCCTGGATGAAGGAGCATGTGAATGACTTTTTTGTCAGGCAGGCAAAAAAGGAAGG
CTACCGCTCACGCGCGGCCTATAAGCTGATGGAGATCGCCGAACGCGATCACTTGTTCAAGCCGGGCATGACGGTAGTAG
ATCTTGGCGCTGCTCCCGGCGGGTGGTCGCAGGTAGCAGCGGAGAAGCTCAAGGGCAAGGGTAGAGCGGTTGCCTTGGAT
ATCCTGGAAATGGCGCCCATATCCGGCGTAACCTTCATTCAGGGAGATTTCCGCGAAGCGTCTGTCCTGGCCGAATTGAA
GGAACAATTGAAGGATTTGCCCGTTGACCTTGTAATTTGCGATATGTCACCTAATATAACGGGCATACGGGTAATTGATC
AAACGCGGGGCATGCACCTGGCGGAGCTGGCTCTCGAGTTTTGTACCGAGCAGTTGAACTCTGGCGGCAACTTCCTCGTC
AAAGTATTTCAAGGTTCTGGTTTTGACGAGTTTTTTCGCGCGATGCGCGCTACATTCCATCGGGTGGTAACACGTAAACC
CTTGGCATCACGGGGTCGGAGCAGTGAAATCTATTTGCTGGGGCTGGGAAAGCGGGATTAG

Upstream 100 bases:

>100_bases
CAGACGACCGGCTTGAGGGCATGTGCGCGCGCCTTGAGCCCGCGTCTGAAATCTGGAGTAAGTGCTAACATATCTTCTTT
CACGACGATCATTTTACGCG

Downstream 100 bases:

>100_bases
ACATTGCGGGTTCGCATGCCAATACAGTGAATATAGGGCTGGGAGGCTGCAGATCTCCACGCGAACCGGGAGGCTGGTAG
CAGGGATAACGGCTATTCTA

Product: ribosomal RNA methyltransferase RrmJ/FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MKRTKTSKAWMKEHVNDFFVRQAKKEGYRSRAAYKLMEIAERDHLFKPGMTVVDLGAAPGGWSQVAAEKLKGKGRAVALD
ILEMAPISGVTFIQGDFREASVLAELKEQLKDLPVDLVICDMSPNITGIRVIDQTRGMHLAELALEFCTEQLNSGGNFLV
KVFQGSGFDEFFRAMRATFHRVVTRKPLASRGRSSEIYLLGLGKRD

Sequences:

>Translated_206_residues
MKRTKTSKAWMKEHVNDFFVRQAKKEGYRSRAAYKLMEIAERDHLFKPGMTVVDLGAAPGGWSQVAAEKLKGKGRAVALD
ILEMAPISGVTFIQGDFREASVLAELKEQLKDLPVDLVICDMSPNITGIRVIDQTRGMHLAELALEFCTEQLNSGGNFLV
KVFQGSGFDEFFRAMRATFHRVVTRKPLASRGRSSEIYLLGLGKRD
>Mature_206_residues
MKRTKTSKAWMKEHVNDFFVRQAKKEGYRSRAAYKLMEIAERDHLFKPGMTVVDLGAAPGGWSQVAAEKLKGKGRAVALD
ILEMAPISGVTFIQGDFREASVLAELKEQLKDLPVDLVICDMSPNITGIRVIDQTRGMHLAELALEFCTEQLNSGGNFLV
KVFQGSGFDEFFRAMRATFHRVVTRKPLASRGRSSEIYLLGLGKRD

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=209, Percent_Identity=36.8421052631579, Blast_Score=143, Evalue=1e-34,
Organism=Homo sapiens, GI29029591, Length=189, Percent_Identity=37.5661375661376, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI29029589, Length=189, Percent_Identity=37.5661375661376, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI7110661, Length=189, Percent_Identity=37.5661375661376, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI194097365, Length=187, Percent_Identity=34.7593582887701, Blast_Score=118, Evalue=3e-27,
Organism=Escherichia coli, GI1789569, Length=204, Percent_Identity=52.9411764705882, Blast_Score=233, Evalue=7e-63,
Organism=Caenorhabditis elegans, GI17554650, Length=189, Percent_Identity=35.4497354497354, Blast_Score=124, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17553474, Length=196, Percent_Identity=35.2040816326531, Blast_Score=121, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI71987550, Length=184, Percent_Identity=35.8695652173913, Blast_Score=115, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71987561, Length=184, Percent_Identity=35.8695652173913, Blast_Score=115, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17553860, Length=184, Percent_Identity=35.8695652173913, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6319535, Length=197, Percent_Identity=34.5177664974619, Blast_Score=124, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6319796, Length=188, Percent_Identity=30.8510638297872, Blast_Score=103, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321302, Length=110, Percent_Identity=35.4545454545455, Blast_Score=74, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21356387, Length=208, Percent_Identity=33.6538461538462, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24647580, Length=194, Percent_Identity=33.5051546391753, Blast_Score=111, Evalue=3e-25,
Organism=Drosophila melanogaster, GI18859957, Length=187, Percent_Identity=31.0160427807487, Blast_Score=104, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24648639, Length=193, Percent_Identity=32.1243523316062, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_NITMU (Q2YBT1)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_411182.1
- ProteinModelPortal:   Q2YBT1
- SMR:   Q2YBT1
- STRING:   Q2YBT1
- GeneID:   3784899
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0482
- eggNOG:   COG0293
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- PhylomeDB:   Q2YBT1
- BioCyc:   NMUL323848:NMUL_A0482-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 23024; Mature: 23024

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: NA

Important sites: ACT_SITE 161-161 BINDING 60-60 BINDING 62-62 BINDING 80-80 BINDING 96-96 BINDING 121-121

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTKTSKAWMKEHVNDFFVRQAKKEGYRSRAAYKLMEIAERDHLFKPGMTVVDLGAAPG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCC
GWSQVAAEKLKGKGRAVALDILEMAPISGVTFIQGDFREASVLAELKEQLKDLPVDLVIC
CHHHHHHHHHCCCCCEEEEHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEEEE
DMSPNITGIRVIDQTRGMHLAELALEFCTEQLNSGGNFLVKVFQGSGFDEFFRAMRATFH
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHH
RVVTRKPLASRGRSSEIYLLGLGKRD
HHHHCCCHHHCCCCCEEEEEECCCCC
>Mature Secondary Structure
MKRTKTSKAWMKEHVNDFFVRQAKKEGYRSRAAYKLMEIAERDHLFKPGMTVVDLGAAPG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCC
GWSQVAAEKLKGKGRAVALDILEMAPISGVTFIQGDFREASVLAELKEQLKDLPVDLVIC
CHHHHHHHHHCCCCCEEEEHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEEEE
DMSPNITGIRVIDQTRGMHLAELALEFCTEQLNSGGNFLVKVFQGSGFDEFFRAMRATFH
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHH
RVVTRKPLASRGRSSEIYLLGLGKRD
HHHHCCCHHHCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA