Click here to switch to the map view.

The map label for this gene is ilvI [H]

Identifier: 82701608

GI number: 82701608

Start: 529376

End: 531133

Strand: Reverse

Name: ilvI [H]

Synonym: Nmul_A0474

Alternate gene names: 82701608

Gene position: 531133-529376 (Counterclockwise)

Preceding gene: 82701609

Following gene: 82701607

Centisome position: 16.68

GC content: 56.03

Gene sequence:

>1758_bases
ATGTTTAATCCAACATATATTGCCGATATTCAACATCTTGAAACGCAGGAAAGCATGAGCGCGGATTTGACGGGCGCCGA
AATTACGGTGCGTTGCTTACAAGAGGAAGGGGTAGAACATATTTTCGGCTATCCCGGGGGCGCTGTGCTGTTCCTGTATG
ACGAATTGTTCAAGCAGGATAAGGTCAGGCATATCCTGGTGCGGCACGAACAGGCCGCGGTACATGCCGCGGACGGCTAT
GCCCGCTCCAGCAACAAGGTAGGGGTGGCACTGGTCACTTCCGGGCCGGGTGTGACCAACGCCGTGACCGGCATCGCCAC
TGCCTATATGGATTCGATTCCCCTCGTCATCATCAGCGGGCAGGTCCCCACCCATGCCATCGGCTTGGACGCGTTCCAGG
AAGTCGATACCGTAGGCATCACGCGTCCCTGCGTGAAGCATAATTTTCTGGTGAAGGATATCGCAGAGCTTGCCGTCACC
ATCAAGAAAGCGTTCTATATCGCTTCGACAGGGCGTCCCGGTCCGGTGCTGGTCGACATCCCGAAGGACGTGAGTCAGCA
GAAAACAAAATTCGTTTATCCCGAGCGTGTCACAATGCGCTCCTATAATCCGAACATCCGGGGGCACGCCGGCCAGATCA
AAAAAGCCGTTCAACTGATCCTGGAAGCCAGGCGTCCGATGATCTACACCGGCGGGGGAGTCATCCTCAGCGACGCCGCT
ACCCGGTTGACGGAACTCGTCCGCCTGCTGCGCTTTCCCTGCACCAACACGTTGATGGGCCTGGGGGGTTATCCGGCAAC
GGACCCCCAGTTTGTGGGCATGCTGGGCATGCATGGCACTTACGAGGCCAACATGGCGATGCAGCATTGCGACGTGCTGG
TGGCTGTAGGTGCCCGTTTCGATGATCGTGTCATCGGCAATCCGAAGCATTTCTATAATCCGGACCGGAAGATCATTCAC
ATCGATATCGATCCTTCCTCCATTTCGAAGCGTGTCAAGGTGGACGTTCCTATCGTCGGCAATGTCCCCGATGTGCTGGA
TGAACTCATAAAACTGCTTGAACTGCGCAAGGAAAAACCCGATCAGACCGCTCTTGACGCCTGGTGGAGCCAGATCGATT
CATGGCGGGAGCGCGACTGTCTCAGGTATGACCGCACAAGCGCGATCATCAAGCCGCAGAGGGTGGTGGAAACGCTCTAC
AAGGTAACGAATGGCGATGCCTTCATTACCTCGGATGTCGGTCAGCACCAGATGTGGGCAGCGCAATTTTACAAATTCGA
CCTGCCGCGGCGATGGATCAATTCCGGAGGGCTTGGAACGATGGGTTTCGGCCTGCCTTCGGCGATGGGTGTCCAGATGG
CTAATCCAGGTGCGAACGTGGCCTGCATTACCGGCGAAGCCAGCATCCAGATGTGCATCCAGGAGCTGTCGACCTGCAAG
CAATATCACCTTCCGCTCAAGATCATCAACCTGAATAACCGCTATATGGGAATGGTGCGGCAGTGGCAGGAATTCTTCCA
TGGCAACCGCTATGCGGAGTCCTACATGGACGCGCTGCCCGATTTCGTGAAGCTGGCGGAGAGTTATGGCCATGTCGGCA
TGCGGATCGAACAGCCCGGCGATGTCGAAGCCGCCTTGCAGGAAGCATTCAAGCTCAAGGACAGGCTCGTGTTCATGGAT
TTCGTCACCGACCAGACCGAAAACGTATTCCCGATGGTGCCGGGCGGCAAGGGTCTGTCTGAAATGATTCTGGTATAA

Upstream 100 bases:

>100_bases
GATAAGGTTTTAGCGCATTTGAAATTGCCCCGTATACCTGTCTACCTGTCGATTCCATACTCGAACTGTTGACCAAAAGT
CACCTAACCGTTACTGTTTA

Downstream 100 bases:

>100_bases
AAACAAATATGCGGCATATCATTTCACTTTTGATGGAAAACGAAGCAGGTGCCCTGTCTCGCGTCGCCGGCCTGTTTTCC
TCACGTGGCTACAATATCGA

Product: acetolactate synthase, large subunit, biosynthetic type

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 585; Mature: 585

Protein sequence:

>585_residues
MFNPTYIADIQHLETQESMSADLTGAEITVRCLQEEGVEHIFGYPGGAVLFLYDELFKQDKVRHILVRHEQAAVHAADGY
ARSSNKVGVALVTSGPGVTNAVTGIATAYMDSIPLVIISGQVPTHAIGLDAFQEVDTVGITRPCVKHNFLVKDIAELAVT
IKKAFYIASTGRPGPVLVDIPKDVSQQKTKFVYPERVTMRSYNPNIRGHAGQIKKAVQLILEARRPMIYTGGGVILSDAA
TRLTELVRLLRFPCTNTLMGLGGYPATDPQFVGMLGMHGTYEANMAMQHCDVLVAVGARFDDRVIGNPKHFYNPDRKIIH
IDIDPSSISKRVKVDVPIVGNVPDVLDELIKLLELRKEKPDQTALDAWWSQIDSWRERDCLRYDRTSAIIKPQRVVETLY
KVTNGDAFITSDVGQHQMWAAQFYKFDLPRRWINSGGLGTMGFGLPSAMGVQMANPGANVACITGEASIQMCIQELSTCK
QYHLPLKIINLNNRYMGMVRQWQEFFHGNRYAESYMDALPDFVKLAESYGHVGMRIEQPGDVEAALQEAFKLKDRLVFMD
FVTDQTENVFPMVPGGKGLSEMILV

Sequences:

>Translated_585_residues
MFNPTYIADIQHLETQESMSADLTGAEITVRCLQEEGVEHIFGYPGGAVLFLYDELFKQDKVRHILVRHEQAAVHAADGY
ARSSNKVGVALVTSGPGVTNAVTGIATAYMDSIPLVIISGQVPTHAIGLDAFQEVDTVGITRPCVKHNFLVKDIAELAVT
IKKAFYIASTGRPGPVLVDIPKDVSQQKTKFVYPERVTMRSYNPNIRGHAGQIKKAVQLILEARRPMIYTGGGVILSDAA
TRLTELVRLLRFPCTNTLMGLGGYPATDPQFVGMLGMHGTYEANMAMQHCDVLVAVGARFDDRVIGNPKHFYNPDRKIIH
IDIDPSSISKRVKVDVPIVGNVPDVLDELIKLLELRKEKPDQTALDAWWSQIDSWRERDCLRYDRTSAIIKPQRVVETLY
KVTNGDAFITSDVGQHQMWAAQFYKFDLPRRWINSGGLGTMGFGLPSAMGVQMANPGANVACITGEASIQMCIQELSTCK
QYHLPLKIINLNNRYMGMVRQWQEFFHGNRYAESYMDALPDFVKLAESYGHVGMRIEQPGDVEAALQEAFKLKDRLVFMD
FVTDQTENVFPMVPGGKGLSEMILV
>Mature_585_residues
MFNPTYIADIQHLETQESMSADLTGAEITVRCLQEEGVEHIFGYPGGAVLFLYDELFKQDKVRHILVRHEQAAVHAADGY
ARSSNKVGVALVTSGPGVTNAVTGIATAYMDSIPLVIISGQVPTHAIGLDAFQEVDTVGITRPCVKHNFLVKDIAELAVT
IKKAFYIASTGRPGPVLVDIPKDVSQQKTKFVYPERVTMRSYNPNIRGHAGQIKKAVQLILEARRPMIYTGGGVILSDAA
TRLTELVRLLRFPCTNTLMGLGGYPATDPQFVGMLGMHGTYEANMAMQHCDVLVAVGARFDDRVIGNPKHFYNPDRKIIH
IDIDPSSISKRVKVDVPIVGNVPDVLDELIKLLELRKEKPDQTALDAWWSQIDSWRERDCLRYDRTSAIIKPQRVVETLY
KVTNGDAFITSDVGQHQMWAAQFYKFDLPRRWINSGGLGTMGFGLPSAMGVQMANPGANVACITGEASIQMCIQELSTCK
QYHLPLKIINLNNRYMGMVRQWQEFFHGNRYAESYMDALPDFVKLAESYGHVGMRIEQPGDVEAALQEAFKLKDRLVFMD
FVTDQTENVFPMVPGGKGLSEMILV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=551, Percent_Identity=27.2232304900181, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI21361361, Length=555, Percent_Identity=24.6846846846847, Blast_Score=143, Evalue=4e-34,
Organism=Escherichia coli, GI87081685, Length=566, Percent_Identity=56.8904593639576, Blast_Score=685, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=564, Percent_Identity=42.0212765957447, Blast_Score=423, Evalue=1e-119,
Organism=Escherichia coli, GI1786717, Length=546, Percent_Identity=33.6996336996337, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI1787096, Length=552, Percent_Identity=29.1666666666667, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1788716, Length=479, Percent_Identity=25.0521920668058, Blast_Score=147, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17531299, Length=565, Percent_Identity=25.8407079646018, Blast_Score=158, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI17531301, Length=565, Percent_Identity=25.8407079646018, Blast_Score=158, Evalue=9e-39,
Organism=Caenorhabditis elegans, GI17542570, Length=575, Percent_Identity=24.8695652173913, Blast_Score=141, Evalue=9e-34,
Organism=Saccharomyces cerevisiae, GI6323755, Length=582, Percent_Identity=42.9553264604811, Blast_Score=457, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6320816, Length=482, Percent_Identity=25.3112033195021, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6323163, Length=506, Percent_Identity=22.7272727272727, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6320123, Length=539, Percent_Identity=21.5213358070501, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6321524, Length=506, Percent_Identity=22.9249011857708, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323073, Length=502, Percent_Identity=21.5139442231076, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19922626, Length=575, Percent_Identity=25.9130434782609, Blast_Score=173, Evalue=4e-43,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 65042; Mature: 65042

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNPTYIADIQHLETQESMSADLTGAEITVRCLQEEGVEHIFGYPGGAVLFLYDELFKQD
CCCCCEEHHHHHHHHHHHHCCCCCCCHHHEEEHHHCCCHHCCCCCCCEEEHHHHHHHHHH
KVRHILVRHEQAAVHAADGYARSSNKVGVALVTSGPGVTNAVTGIATAYMDSIPLVIISG
HHHHHHHCCHHHHEEECCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEC
QVPTHAIGLDAFQEVDTVGITRPCVKHNFLVKDIAELAVTIKKAFYIASTGRPGPVLVDI
CCCCCEECCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEEC
PKDVSQQKTKFVYPERVTMRSYNPNIRGHAGQIKKAVQLILEARRPMIYTGGGVILSDAA
CCCCCHHHHEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCEEEHHHH
TRLTELVRLLRFPCTNTLMGLGGYPATDPQFVGMLGMHGTYEANMAMQHCDVLVAVGARF
HHHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHEECCCCCCHHHHHHHHHHEEEEECCCC
DDRVIGNPKHFYNPDRKIIHIDIDPSSISKRVKVDVPIVGNVPDVLDELIKLLELRKEKP
CCCCCCCCHHHCCCCCEEEEEEECHHHHCCEEEEECEEECCCHHHHHHHHHHHHHHHCCC
DQTALDAWWSQIDSWRERDCLRYDRTSAIIKPQRVVETLYKVTNGDAFITSDVGQHQMWA
CHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHH
AQFYKFDLPRRWINSGGLGTMGFGLPSAMGVQMANPGANVACITGEASIQMCIQELSTCK
HHHHHCCCCHHHHCCCCCCCCCCCCCCCCCEEECCCCCCEEEEECCHHHHHHHHHHHHHH
QYHLPLKIINLNNRYMGMVRQWQEFFHGNRYAESYMDALPDFVKLAESYGHVGMRIEQPG
HHCCCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCC
DVEAALQEAFKLKDRLVFMDFVTDQTENVFPMVPGGKGLSEMILV
CHHHHHHHHHHHHHHEEEEEECCCCCCCEEEECCCCCCHHHHHCC
>Mature Secondary Structure
MFNPTYIADIQHLETQESMSADLTGAEITVRCLQEEGVEHIFGYPGGAVLFLYDELFKQD
CCCCCEEHHHHHHHHHHHHCCCCCCCHHHEEEHHHCCCHHCCCCCCCEEEHHHHHHHHHH
KVRHILVRHEQAAVHAADGYARSSNKVGVALVTSGPGVTNAVTGIATAYMDSIPLVIISG
HHHHHHHCCHHHHEEECCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEC
QVPTHAIGLDAFQEVDTVGITRPCVKHNFLVKDIAELAVTIKKAFYIASTGRPGPVLVDI
CCCCCEECCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEEC
PKDVSQQKTKFVYPERVTMRSYNPNIRGHAGQIKKAVQLILEARRPMIYTGGGVILSDAA
CCCCCHHHHEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCEEEHHHH
TRLTELVRLLRFPCTNTLMGLGGYPATDPQFVGMLGMHGTYEANMAMQHCDVLVAVGARF
HHHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHEECCCCCCHHHHHHHHHHEEEEECCCC
DDRVIGNPKHFYNPDRKIIHIDIDPSSISKRVKVDVPIVGNVPDVLDELIKLLELRKEKP
CCCCCCCCHHHCCCCCEEEEEEECHHHHCCEEEEECEEECCCHHHHHHHHHHHHHHHCCC
DQTALDAWWSQIDSWRERDCLRYDRTSAIIKPQRVVETLYKVTNGDAFITSDVGQHQMWA
CHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHH
AQFYKFDLPRRWINSGGLGTMGFGLPSAMGVQMANPGANVACITGEASIQMCIQELSTCK
HHHHHCCCCHHHHCCCCCCCCCCCCCCCCCEEECCCCCCEEEEECCHHHHHHHHHHHHHH
QYHLPLKIINLNNRYMGMVRQWQEFFHGNRYAESYMDALPDFVKLAESYGHVGMRIEQPG
HHCCCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCC
DVEAALQEAFKLKDRLVFMDFVTDQTENVFPMVPGGKGLSEMILV
CHHHHHHHHHHHHHHEEEEEECCCCCCCEEEECCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]